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stalk_of_fennel
Stranger


Registered: 12/05/09
Posts: 122
Last seen: 9 years, 8 months
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Pressure cook agar in petri dishes
#11592192 - 12/05/09 07:07 PM (14 years, 2 months ago) |
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Ive read a few instructionals claiming that you can pressure cook your agar medium in the petri dish. This seems a lot better (easier and less contamination) then pouring the medium into the petri dishes afterwards. Most of the instructions I've read for making petri dishes have you pour the agar after it's been sterilized...
I'm guessing that pressure cooking your medium in the petri dish doesn't work out or everyone would be doing it.
Anyone have experience with this?
SF
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Shea25
Just some guy



Registered: 01/27/09
Posts: 7,772
Loc: Westcoast Canada
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If there plastic dishes you will end up with a lump of melted plastic. I would stick with disposable dishes and just PC the agar in a bottle with a filter on it. Glass dishes can be a pain
Edited by Shea25 (12/05/09 07:10 PM)
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Lennybernadino
Amazon grower


Registered: 09/16/09
Posts: 770
Loc: Iquitos, Peru
Last seen: 15 days, 10 hours
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Re: Pressure cook agar in petri dishes [Re: Shea25]
#11592222 - 12/05/09 07:11 PM (14 years, 2 months ago) |
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why can glass petri dishes be a pain?
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frankenstoen

Registered: 01/26/08
Posts: 385
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Re: Pressure cook agar in petri dishes [Re: Shea25]
#11592226 - 12/05/09 07:12 PM (14 years, 2 months ago) |
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Shea25
Just some guy



Registered: 01/27/09
Posts: 7,772
Loc: Westcoast Canada
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Cleaning them,Making sure not to break them. Slippery with alcohol soaked latex glove. Having to PC them to sterilize them. The cost of disposable dishes is SUPER cheap
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badman


Registered: 06/14/06
Posts: 4,039
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Re: Pressure cook agar in petri dishes [Re: Shea25]
#11592669 - 12/05/09 08:10 PM (14 years, 2 months ago) |
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glass is only a bitch when you get contams, thankfully thats a rare occurance (for moi) but you can use then 10000s of times. IMO glass beats plastic
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Shea25
Just some guy



Registered: 01/27/09
Posts: 7,772
Loc: Westcoast Canada
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Re: Pressure cook agar in petri dishes [Re: badman]
#11592688 - 12/05/09 08:13 PM (14 years, 2 months ago) |
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Here where I live its like 5 bucks a glass dish I would need so many to do some Isolations where plastic is super cheap
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stalk_of_fennel
Stranger


Registered: 12/05/09
Posts: 122
Last seen: 9 years, 8 months
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Re: Pressure cook agar in petri dishes [Re: Shea25]
#11592729 - 12/05/09 08:18 PM (14 years, 2 months ago) |
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well... I just tried it and i see the problem.
i used glass petri dishes and it boils over. a the agar medium is all over the inside and outside of the petri dishes now. it seems to me that it's a contamination highway.
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noobieshroomie
Back again




Registered: 07/18/08
Posts: 12,769
Loc: Not Too Sure
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personally i dont find it to be that much of a pain the only problem i run into is condensation on the lids done in a GB takes 5 min or less for 20 dishes heres a few i did last night

-noobie-
-------------------- AMU Best Thread Ever CapZilla said: not sure what GE and FAE are but i should probably get some. Citric said: Your signature is wrong on colonization temps! GOOD JUDGMENT COMES FROM EXPERIENCE EXPERIENCE COMES FROM BAD JUDGMENT ROOM TEMP 70-75 IS BEST FOR COLONIZATION Thank you mycochef
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hardcorekid83
hardcorekid



Registered: 07/25/09
Posts: 250
Loc: pittsburgh pa
Last seen: 3 years, 9 months
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Quote:
noobieshroomie said: personally i dont find it to be that much of a pain the only problem i run into is condensation on the lids done in a GB takes 5 min or less for 20 dishes heres a few i did last night

-noobie-
noobie, whats a gb?
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noobieshroomie
Back again




Registered: 07/18/08
Posts: 12,769
Loc: Not Too Sure
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Re: Pressure cook agar in petri dishes [Re: hardcorekid83]
#11593172 - 12/05/09 09:47 PM (14 years, 2 months ago) |
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a glove box
-noobie-
-------------------- AMU Best Thread Ever CapZilla said: not sure what GE and FAE are but i should probably get some. Citric said: Your signature is wrong on colonization temps! GOOD JUDGMENT COMES FROM EXPERIENCE EXPERIENCE COMES FROM BAD JUDGMENT ROOM TEMP 70-75 IS BEST FOR COLONIZATION Thank you mycochef
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Cloneufc
Master Exploder!



Registered: 11/15/07
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All you have to do with glass petri dishes is get automotive wet sand paper and cut it out into circles the size of the dish on the bottom. Then you just glue it to the bottom and you'll never have to worry about a glass petri dish slipping again.
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RogerRabbit
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Registered: 03/26/03
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Loc: Seattle
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Re: Pressure cook agar in petri dishes [Re: Cloneufc]
#11593631 - 12/05/09 11:31 PM (14 years, 2 months ago) |
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The heavy glass lids slip out of your gloved hands when working too.
To the original poster, you don't want to sterilize the agar in glass dishes, as you found out. You also have many more vectors of contamination than you do sterilizing in a whiskey bottle with a tyvek lid, and then pouring under controlled conditions later.
Get a box of 500 pre sterilized petri dishes for the cost of 20 glass ones. RR
-------------------- Download Let's Grow Mushrooms semper in excretia sumus solim profundum variat "I've never had a failed experiment. I've only discovered 10,000 methods which do not work." Thomas Edison
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