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Offlinestalk_of_fennel
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Pressure cook agar in petri dishes
    #11592192 - 12/05/09 07:07 PM (14 years, 2 months ago)

Ive read a few instructionals claiming that you can pressure cook your agar medium in the petri dish.  This seems a lot better (easier and less contamination) then pouring the medium into the petri dishes afterwards.  Most of the instructions I've read for making petri dishes have you pour the agar after it's been sterilized...

I'm guessing that pressure cooking your medium in the petri dish doesn't work out or everyone would be doing it.

Anyone have experience with this? 

SF


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InvisibleShea25
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Re: Pressure cook agar in petri dishes [Re: stalk_of_fennel]
    #11592208 - 12/05/09 07:09 PM (14 years, 2 months ago)

If there plastic dishes you will end up with a lump of melted plastic. I would stick with disposable dishes and just PC the agar in a bottle with a filter on it. Glass dishes can be a pain


Edited by Shea25 (12/05/09 07:10 PM)


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OfflineLennybernadino
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Re: Pressure cook agar in petri dishes [Re: Shea25]
    #11592222 - 12/05/09 07:11 PM (14 years, 2 months ago)

why can glass petri dishes be a pain?


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Re: Pressure cook agar in petri dishes [Re: Shea25]
    #11592226 - 12/05/09 07:12 PM (14 years, 2 months ago)



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InvisibleShea25
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Re: Pressure cook agar in petri dishes [Re: Lennybernadino]
    #11592234 - 12/05/09 07:13 PM (14 years, 2 months ago)

Cleaning them,Making sure not to break them. Slippery with alcohol soaked latex glove. Having to PC them to sterilize them. The cost of disposable dishes is SUPER cheap


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Invisiblebadman
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Re: Pressure cook agar in petri dishes [Re: Shea25]
    #11592669 - 12/05/09 08:10 PM (14 years, 2 months ago)

glass is only a bitch when you get contams, thankfully thats a rare occurance (for moi) but you can use then 10000s of times. IMO glass beats plastic


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InvisibleShea25
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Re: Pressure cook agar in petri dishes [Re: badman]
    #11592688 - 12/05/09 08:13 PM (14 years, 2 months ago)

Here where I live its like 5 bucks a glass dish I would need so many to do some Isolations where plastic is super cheap


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Offlinestalk_of_fennel
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Re: Pressure cook agar in petri dishes [Re: Shea25]
    #11592729 - 12/05/09 08:18 PM (14 years, 2 months ago)

well... I just tried it and i see the problem.

i used glass petri dishes and it boils over.  a the agar medium is all over the inside and outside of the petri dishes now.  it seems to me that it's a contamination highway.


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Invisiblenoobieshroomie
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Re: Pressure cook agar in petri dishes [Re: stalk_of_fennel]
    #11592768 - 12/05/09 08:23 PM (14 years, 2 months ago)

personally i dont find it to be that much of a pain
the only problem i run into is condensation on the lids
done in a GB takes 5 min or less for 20 dishes
heres a few i did last night


    -noobie-


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Offlinehardcorekid83
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Re: Pressure cook agar in petri dishes [Re: noobieshroomie]
    #11593135 - 12/05/09 09:37 PM (14 years, 2 months ago)

Quote:

noobieshroomie said:
personally i dont find it to be that much of a pain
the only problem i run into is condensation on the lids
done in a GB takes 5 min or less for 20 dishes
heres a few i did last night


    -noobie-



noobie, whats a gb?


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Re: Pressure cook agar in petri dishes [Re: hardcorekid83]
    #11593172 - 12/05/09 09:47 PM (14 years, 2 months ago)



a glove box

  -noobie-


--------------------
AMU

Best Thread Ever
CapZilla said:
not sure what GE and FAE are but i should probably get some.

Citric said:
Your signature is wrong on colonization temps!

GOOD JUDGMENT COMES FROM EXPERIENCE
EXPERIENCE COMES FROM BAD JUDGMENT

ROOM TEMP 70-75 IS BEST FOR COLONIZATION
Thank you mycochef


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OfflineCloneufc
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Re: Pressure cook agar in petri dishes [Re: noobieshroomie]
    #11593203 - 12/05/09 09:54 PM (14 years, 2 months ago)

All you have to do with glass petri dishes is get automotive wet sand paper and cut it out into circles the size of the dish on the bottom. Then you just glue it to the bottom and you'll never have to worry about a glass petri dish slipping again.


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OfflineRogerRabbitM
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Re: Pressure cook agar in petri dishes [Re: Cloneufc]
    #11593631 - 12/05/09 11:31 PM (14 years, 2 months ago)

The heavy glass lids slip out of your gloved hands when working too.

To the original poster, you don't want to sterilize the agar in glass dishes, as you found out.  You also have many more vectors of contamination than you do sterilizing in a whiskey bottle with a tyvek lid, and then pouring under controlled conditions later.

Get a box of 500 pre sterilized petri dishes for the cost of 20 glass ones.
RR


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