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Shea25
Just some guy



Registered: 01/27/09
Posts: 7,772
Loc: Westcoast Canada
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Pf tek done right TEK 71
#11585613 - 12/04/09 06:28 PM (14 years, 1 month ago) |
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PF tek Things needed
1/2 pint wide mouth canning jars Pot/Pressure cooker Vermiculite Perlite Brown rice/Brow Rice Flour Tin foil 70% Iso alcohol Plastic Tote 6500K florescent bulb 1/4 inch drill bit/Drill Hammer/Nail Mixing bowl and spoon Gypsum Spore syringe Latex gloves Dust mask Lighter
Preparing the jar
Take the lids and using a nail and hammer or a 1/8 drill bit, drill 4 holes near the rim of the lids nicely spaced out. Depending what way you use you lids i use mine rubber side down just punch the holes so they don’t leave burrs pointing towards you.
The basic recipe for 5 PF jars is this 2 parts vermiculite 1 part water and 1 part BRF (additionally you can add 1 tablespoon of gypsum for 5 jars)
In a bowl add the 2 cups of vermiculite (and gypsum if you use it) now add the 1 cup of water, mix really well. Now add the 1 cup of Brown rice flour (you can make your own by grinding brow rice in a coffee grinder) Mix this really really well. Now load this into the 5 canning jar equally, making sure not to compress the mixture in the jars you want it nice and airy. You will be leaving a 1/2 inch space at the tops of the jars for the dry vermiculite layer.
Take a paper towel and wipe the 1/2inch space you left at the tops of the jars making sure to wipe away any BRF mixture or moisture away. Now top off the jars with dry vermiculite, screw on the lids and cover the tops the lids with tin foil. The tin foil is there to keep any moisture from entering the jars while there sterilizing.









Sterilizing
Now you want to take you PC or (pot with a tight fitting lid) and fill the bottom of it with jar lid rings and tin foil this is to keep the bottom of your jars off the bottom of the PC/Pot so they don’t burn Some PC’s come with trays to keep the jar already off the bottom use that if you can. Once that is done put the jars into the PC/pot and fill it with cold water just almost to the bottom of the jars. Now put on the lid of the PC/Pot and put the heat on High. Once the rocker on top of the PC starts to rock turn the heat right down to just keep it to a slight rock and start your 60 minute count down, If you’re using a pot once it come to a boil turn it down to a slight simmer and start your 90 minute count down. After the 60(PC) or 90(Pot) minutes are up turn off the heat and walk away let them cool down over night or for 8 hours.



Inoculation
Once the jars are cooled down put them in a draft free room along with the 70% Iso,Lighter,spore syringe . Make sure the rooms windows are shut and AC or heater turned off. Wash your hands and arms really good. Now put of the gloves and mask Take the foil lids off the jars you won’t need them anymore. Now rub your hand down with the 70% ISO wait a few seconds for it to evaporate. Now shake the syringe really well, using the lighter heat the needle of the syringe till its red hot now proceed to inoculate the jars using a 1/4 cc of solution per hole inoculating towards the glass. That’s 1 cc per jar. After every jar heat the needle back till its red hot and repeat.



Incubation
Now put the jars away in a closet of shelf at room temp they will start to colonize in a few days. Keep an eye on them every 4 days or so. Once they appear to be 100% colonized let them sit for another week to consolidate.

Birthing and dunk
Once the jars are finished their 1 week consolidation it’s ready to get them out of the jars. Open the jars get rid of the dry vermiculite layer now slap the jars (upside down) onto a wood cutting board or counter top, trying to coax the cakes out of the jars. Once out rinse them off and submerge in cool water for 24 hours making sure they stay submerged. After 24 hours rinse them again and now roll the cake in dry vermiculite making sure to cover the cake well tops and bottoms. I find busting up some of the verm for the dry roll up alittle finer helps.







Fruiting
Build one of these http://www.mushroomvideos.com/Terrarium-Tek
I personally use a PMP
Mist your cakes 3-4 times a day and fan right after, fan 5-6 times a day. Water evaporating off your cakes is a major pinning trigger. Give them a 12/12 light schedule using a 6500K Florescent bulb (daylight or cool blue). Once they have pinned and grown and all picked, dunk them for 24 hours again this time no dry roll in vermiculite and put back in the Shotgun Fruiting Chamber.
Good Luck
Tips
Gypsum really helps the development of the fruit bodies I recommend using it Room temp 70-75 is perfect for incubation and fruiting. There’s no need to keep the dunking cakes in the fridge Mist and Fan Mist and Fan Use short wide mouth 1/2 pint jars Dont cover the lids after inoculation with anything example, Foil/tape Give them love
Results in this Golden Teacher

This was 2 cakes first flush, came just under 19 grams dry. Oak Ridge

Heres another set of cakes


Results may vary
Here is a How to on sterilizing a syringe to make up a spore syringe
How to sterilize a syringe with just a pot water and syringe
Thing needed.
A pot Empty Syringe Water(tap can work)
Fill the pot with cold water. turn the burner on High and wait for the water to come to a full boil. now start a 10 minute countdown and keep the water at a full boil.
After 10 minutes take the empty syringe and suck back the boiling water and let it sit in the syringe for 1 minute then expel the water in a sink(not back in the pot)
Repeat 4 more times on the last time keep the water in the syringe cap it and let it cool down.
Hope this helps.





 x4

Notes: Use a Q-tip and 70% alcohol to clean the inside of the cap I have found even after months and months they will stay sterile No need to store in the fridge Never try to cool them down fast in the fridge
Edited by Shea25 (06/16/10 10:52 PM)
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Shea25
Just some guy



Registered: 01/27/09
Posts: 7,772
Loc: Westcoast Canada
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Re: Pf tek done right [Re: badman] 3
#11585674 - 12/04/09 06:37 PM (14 years, 1 month ago) |
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Using this method I was constantly getting 6-9 gram first flushes. Around 13 grams dry per cake. the one in my avatar came out to 7.6 dry first flush
Edited by Shea25 (12/04/09 06:37 PM)
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Shea25
Just some guy



Registered: 01/27/09
Posts: 7,772
Loc: Westcoast Canada
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It sure does I have done TONNES of PF's and the dry verm barrier has not let me down. You will see. I have told so many people when there asking why are my jars going so slow and it because people cover the holes. Theres just no need to, or whats the point of the dry verm? might as well build self healing lids with a tyvek GE port
Edited by Shea25 (12/04/09 07:24 PM)
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Shea25
Just some guy



Registered: 01/27/09
Posts: 7,772
Loc: Westcoast Canada
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lol I would say start saving that micropore tape for other applications. Its a waste to use it over the innc holes. Its not the cheapest stuff
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Shea25
Just some guy



Registered: 01/27/09
Posts: 7,772
Loc: Westcoast Canada
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The top dry verm layer is a must. I have a 99.9% successful PF rate. Only time I had a contam was when I tried an experimental technique
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Shea25
Just some guy



Registered: 01/27/09
Posts: 7,772
Loc: Westcoast Canada
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Re: Pf tek done right TEK [Re: Shea25] 2
#11586249 - 12/04/09 07:57 PM (14 years, 1 month ago) |
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Here are some Self healing Filtered GE port PF tek lids I made

These also with a dry verm barrier your basically 100% not going to get any contams from the outside at least
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Shea25
Just some guy



Registered: 01/27/09
Posts: 7,772
Loc: Westcoast Canada
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Looking sweet . A little advice thoe i would not be putting my jars on the ground especially carpet. Most mold spores and contams are only 1 foot from the ground. Im sure you fine but its good to know
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Shea25
Just some guy



Registered: 01/27/09
Posts: 7,772
Loc: Westcoast Canada
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Gas Exchange is most certainly needed for colonization. FAE is not
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Shea25
Just some guy



Registered: 01/27/09
Posts: 7,772
Loc: Westcoast Canada
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I personally would not have it on the ground that just me thoe. im not saying it will contam if you do, but might as well lessen the chances. I like to keep all my stuff elevated off the ground.
Edited by Shea25 (12/04/09 08:14 PM)
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Shea25
Just some guy



Registered: 01/27/09
Posts: 7,772
Loc: Westcoast Canada
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Re: Pf tek done right [Re: Goat Boy]
#11586414 - 12/04/09 08:21 PM (14 years, 1 month ago) |
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7-10 days, thats from right after the dry roll to first harvest. 3-5 days to pin 4-5 days to mature
Edited by Shea25 (02/05/10 12:26 PM)
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Shea25
Just some guy



Registered: 01/27/09
Posts: 7,772
Loc: Westcoast Canada
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Re: Pf tek done right [Re: Goat Boy]
#11586456 - 12/04/09 08:27 PM (14 years, 1 month ago) |
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I use a modified PMP for a fruiting chamber. Does cubes and cyans great. Keeps constant FAE and high RH. When I have time I still manually fan and mist. I also use a 4 foot double bulb florescent fixture with 2 6500K bulbs in it, using a 12/12 lighting schedule.
Example


The chamber does not look like that now I changed it a round a bit no more rock in the bottom just more perlite and water
Edited by Shea25 (12/04/09 08:32 PM)
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Shea25
Just some guy



Registered: 01/27/09
Posts: 7,772
Loc: Westcoast Canada
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Re: Pf tek done right [Re: cyantific]
#11586736 - 12/04/09 09:03 PM (14 years, 1 month ago) |
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You could do that or put the mono in a bag and close it up while its colonizing
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Shea25
Just some guy



Registered: 01/27/09
Posts: 7,772
Loc: Westcoast Canada
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Personally I like PMP's better when there built right. They can handle many different types of mushrooms giving them lots of Fresh air. Species like cyans and oyster need lots of fresh air. I have also used a Shotgun but I must say my PMP beats it hands down. Economically speaking Shotguns are better but if you have the money might as well build a PMP that if you want to get into other species. The main reason I built it was for cyans. But now since I am not doing any active grows for awhile it will suit my needs for oysters and other edibles
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Shea25
Just some guy



Registered: 01/27/09
Posts: 7,772
Loc: Westcoast Canada
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Yea it is its High gloss HI temp paint
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Shea25
Just some guy



Registered: 01/27/09
Posts: 7,772
Loc: Westcoast Canada
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Add the gypsum to the dry vermiculite and mix well then add the water and mix then add the BRF and mix well.
Gypsum is great stuff
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Shea25
Just some guy



Registered: 01/27/09
Posts: 7,772
Loc: Westcoast Canada
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Re: Pf tek done right [Re: nastos]
#11592012 - 12/05/09 06:40 PM (14 years, 1 month ago) |
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Yea in cases like that where you know it can be a problem use the micropore tape. But for the masses that does not happen.
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Shea25
Just some guy



Registered: 01/27/09
Posts: 7,772
Loc: Westcoast Canada
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No Calcium carbonate(CaCO3) Is used to buffer casing to a base/alkaline to prevent molding. That would be a bad thing to add since mushrooms prefer a acidic sub. Calcium carbonate would slow things down
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Shea25
Just some guy



Registered: 01/27/09
Posts: 7,772
Loc: Westcoast Canada
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Nope not really just go buy a cheap plain piece of dry wall and smash it up then powder it and there ya go.
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Shea25
Just some guy



Registered: 01/27/09
Posts: 7,772
Loc: Westcoast Canada
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Re: Pf tek done right [Re: komodo]
#11604630 - 12/07/09 04:13 PM (14 years, 1 month ago) |
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because they have a big hole(bigger 1/4 inch) that has a breathable filter for GE
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Shea25
Just some guy



Registered: 01/27/09
Posts: 7,772
Loc: Westcoast Canada
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1 tablespoon for 5 jars is perfect
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