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Shea25
Just some guy



Registered: 01/27/09
Posts: 7,772
Loc: Westcoast Canada
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Pf tek done right TEK 71
#11585613 - 12/04/09 06:28 PM (14 years, 1 month ago) |
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PF tek Things needed
1/2 pint wide mouth canning jars Pot/Pressure cooker Vermiculite Perlite Brown rice/Brow Rice Flour Tin foil 70% Iso alcohol Plastic Tote 6500K florescent bulb 1/4 inch drill bit/Drill Hammer/Nail Mixing bowl and spoon Gypsum Spore syringe Latex gloves Dust mask Lighter
Preparing the jar
Take the lids and using a nail and hammer or a 1/8 drill bit, drill 4 holes near the rim of the lids nicely spaced out. Depending what way you use you lids i use mine rubber side down just punch the holes so they don’t leave burrs pointing towards you.
The basic recipe for 5 PF jars is this 2 parts vermiculite 1 part water and 1 part BRF (additionally you can add 1 tablespoon of gypsum for 5 jars)
In a bowl add the 2 cups of vermiculite (and gypsum if you use it) now add the 1 cup of water, mix really well. Now add the 1 cup of Brown rice flour (you can make your own by grinding brow rice in a coffee grinder) Mix this really really well. Now load this into the 5 canning jar equally, making sure not to compress the mixture in the jars you want it nice and airy. You will be leaving a 1/2 inch space at the tops of the jars for the dry vermiculite layer.
Take a paper towel and wipe the 1/2inch space you left at the tops of the jars making sure to wipe away any BRF mixture or moisture away. Now top off the jars with dry vermiculite, screw on the lids and cover the tops the lids with tin foil. The tin foil is there to keep any moisture from entering the jars while there sterilizing.









Sterilizing
Now you want to take you PC or (pot with a tight fitting lid) and fill the bottom of it with jar lid rings and tin foil this is to keep the bottom of your jars off the bottom of the PC/Pot so they don’t burn Some PC’s come with trays to keep the jar already off the bottom use that if you can. Once that is done put the jars into the PC/pot and fill it with cold water just almost to the bottom of the jars. Now put on the lid of the PC/Pot and put the heat on High. Once the rocker on top of the PC starts to rock turn the heat right down to just keep it to a slight rock and start your 60 minute count down, If you’re using a pot once it come to a boil turn it down to a slight simmer and start your 90 minute count down. After the 60(PC) or 90(Pot) minutes are up turn off the heat and walk away let them cool down over night or for 8 hours.



Inoculation
Once the jars are cooled down put them in a draft free room along with the 70% Iso,Lighter,spore syringe . Make sure the rooms windows are shut and AC or heater turned off. Wash your hands and arms really good. Now put of the gloves and mask Take the foil lids off the jars you won’t need them anymore. Now rub your hand down with the 70% ISO wait a few seconds for it to evaporate. Now shake the syringe really well, using the lighter heat the needle of the syringe till its red hot now proceed to inoculate the jars using a 1/4 cc of solution per hole inoculating towards the glass. That’s 1 cc per jar. After every jar heat the needle back till its red hot and repeat.



Incubation
Now put the jars away in a closet of shelf at room temp they will start to colonize in a few days. Keep an eye on them every 4 days or so. Once they appear to be 100% colonized let them sit for another week to consolidate.

Birthing and dunk
Once the jars are finished their 1 week consolidation it’s ready to get them out of the jars. Open the jars get rid of the dry vermiculite layer now slap the jars (upside down) onto a wood cutting board or counter top, trying to coax the cakes out of the jars. Once out rinse them off and submerge in cool water for 24 hours making sure they stay submerged. After 24 hours rinse them again and now roll the cake in dry vermiculite making sure to cover the cake well tops and bottoms. I find busting up some of the verm for the dry roll up alittle finer helps.







Fruiting
Build one of these http://www.mushroomvideos.com/Terrarium-Tek
I personally use a PMP
Mist your cakes 3-4 times a day and fan right after, fan 5-6 times a day. Water evaporating off your cakes is a major pinning trigger. Give them a 12/12 light schedule using a 6500K Florescent bulb (daylight or cool blue). Once they have pinned and grown and all picked, dunk them for 24 hours again this time no dry roll in vermiculite and put back in the Shotgun Fruiting Chamber.
Good Luck
Tips
Gypsum really helps the development of the fruit bodies I recommend using it Room temp 70-75 is perfect for incubation and fruiting. There’s no need to keep the dunking cakes in the fridge Mist and Fan Mist and Fan Use short wide mouth 1/2 pint jars Dont cover the lids after inoculation with anything example, Foil/tape Give them love
Results in this Golden Teacher

This was 2 cakes first flush, came just under 19 grams dry. Oak Ridge

Heres another set of cakes


Results may vary
Here is a How to on sterilizing a syringe to make up a spore syringe
How to sterilize a syringe with just a pot water and syringe
Thing needed.
A pot Empty Syringe Water(tap can work)
Fill the pot with cold water. turn the burner on High and wait for the water to come to a full boil. now start a 10 minute countdown and keep the water at a full boil.
After 10 minutes take the empty syringe and suck back the boiling water and let it sit in the syringe for 1 minute then expel the water in a sink(not back in the pot)
Repeat 4 more times on the last time keep the water in the syringe cap it and let it cool down.
Hope this helps.





 x4

Notes: Use a Q-tip and 70% alcohol to clean the inside of the cap I have found even after months and months they will stay sterile No need to store in the fridge Never try to cool them down fast in the fridge
Edited by Shea25 (06/16/10 10:52 PM)
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Shea25
Just some guy



Registered: 01/27/09
Posts: 7,772
Loc: Westcoast Canada
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Re: Pf tek done right TEK [Re: Shea25] 2
#11586249 - 12/04/09 07:57 PM (14 years, 1 month ago) |
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Here are some Self healing Filtered GE port PF tek lids I made

These also with a dry verm barrier your basically 100% not going to get any contams from the outside at least
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Cyanesense
AMU's Fish Killa



Registered: 10/13/09
Posts: 1,363
Loc: PNW
Last seen: 11 years, 7 months
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 These are jars I made up 2 weeks ago, I took this picture early yesterday morning. The Taz (on the left) showed and started growth before the Cambos(on the right.) but the cambos have EXPLODED past the taz. a very fast, agressive colonizer once established. cant wait to get some LC started!!!c'mon payday
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Everything I say is totally false - and all my pictures were stolen from SWIM.
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Shea25
Just some guy



Registered: 01/27/09
Posts: 7,772
Loc: Westcoast Canada
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Re: Pf tek done right [Re: Goat Boy]
#11586456 - 12/04/09 08:27 PM (14 years, 1 month ago) |
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I use a modified PMP for a fruiting chamber. Does cubes and cyans great. Keeps constant FAE and high RH. When I have time I still manually fan and mist. I also use a 4 foot double bulb florescent fixture with 2 6500K bulbs in it, using a 12/12 lighting schedule.
Example


The chamber does not look like that now I changed it a round a bit no more rock in the bottom just more perlite and water
Edited by Shea25 (12/04/09 08:32 PM)
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Shea25
Just some guy



Registered: 01/27/09
Posts: 7,772
Loc: Westcoast Canada
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If you measure the surface area of a single cake you will see what I mean by it has the greatest fruiting surface area. Also you dont have to baby cakes heck I got this from, just fruiting it in a pop bottle maybe misting it twice a day

Edited by Shea25 (01/25/10 01:19 PM)
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Dionili
Second Rate Mycologist



Registered: 08/18/09
Posts: 2,194
Loc: Between a Rock and a Hard...
Last seen: 4 months, 20 days
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Quote:
libertaire said: Indeed, unless you do it really perfectly, pint jars have a high chance of drying out before full colonization. Other than that though, I would think that a pint cake would do better overall than a half pint cake, just because of it's doubled mass. If you're new though, I wouldn't risk it.
I've only had one pint ever fruit (the rest got some sort of contam)
Though, it did give me the biggest shrooms i've ever flushed lol.
one massive pin 
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Shea25
Just some guy



Registered: 01/27/09
Posts: 7,772
Loc: Westcoast Canada
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Quote:
santaclausmush said:
Quote:
libertaire said: Indeed, unless you do it really perfectly, pint jars have a high chance of drying out before full colonization. Other than that though, I would think that a pint cake would do better overall than a half pint cake, just because of it's doubled mass. If you're new though, I wouldn't risk it.
thats what im saying i do pint jars they take longer they dont dry out or nothing if u keep em in the closet. and when there done i never measured but the yeild had to be more than halfs.
I guess you have never had half pints like this then

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Cyanesense
AMU's Fish Killa



Registered: 10/13/09
Posts: 1,363
Loc: PNW
Last seen: 11 years, 7 months
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PoorMansPod is the way to go 
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Everything I say is totally false - and all my pictures were stolen from SWIM.
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santaclausmush
Stranger


Registered: 02/25/08
Posts: 263
Last seen: 10 years, 11 months
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Re: Pf tek done right TEK [Re: Shea25]
#12217259 - 03/17/10 07:16 AM (13 years, 10 months ago) |
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hey shea i had a hpoo verm gypsum and substrate lasagna tray in for three weeks now and its still not 100% colonized what am i doing wrong why is it taking so long.
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Shea25
Just some guy



Registered: 01/27/09
Posts: 7,772
Loc: Westcoast Canada
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Quote:
santaclausmush said: hey shea i had a hpoo verm gypsum and substrate lasagna tray in for three weeks now and its still not 100% colonized what am i doing wrong why is it taking so long.

I have no clue with out knowing more about it. My friend recently had a stalled bulk tray. So he just went ahead and cased it and it seems to be working out alright
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santaclausmush
Stranger


Registered: 02/25/08
Posts: 263
Last seen: 10 years, 11 months
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Re: Pf tek done right TEK [Re: Shea25]
#12219883 - 03/17/10 04:54 PM (13 years, 10 months ago) |
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Quote:
Shea25 said: Here are some Self healing Filtered GE port PF tek lids I made

These also with a dry verm barrier your basically 100% not going to get any contams from the outside at least
what is that stuff u put on the lid.
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santaclausmush
Stranger


Registered: 02/25/08
Posts: 263
Last seen: 10 years, 11 months
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Re: Pf tek done right TEK [Re: Shea25]
#12229330 - 03/19/10 06:04 AM (13 years, 10 months ago) |
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Quote:
Shea25 said: Here are some Self healing Filtered GE port PF tek lids I made

These also with a dry verm barrier your basically 100% not going to get any contams from the outside at least
what do u do for air exchange or gas . with a lid that seals like that theres no air exchange
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santaclausmush
Stranger


Registered: 02/25/08
Posts: 263
Last seen: 10 years, 11 months
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Re: Pf tek done right TEK [Re: Shea25]
#12229829 - 03/19/10 09:15 AM (13 years, 10 months ago) |
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my bad i couldnt see the filter. shea did u get my messages i sent to u? i had a few good questions. did u get em.. also. i have organic rye berries in a jar totally colonized. i want to take the liquid in the jar and use it to make LC. is that possible? someone had a teck adding sterilized water to rye and sucking theliquid out was used to inoculate a brf cake. so why cant it be used to make LC. ILL GET A PC RIGHT NOW. so heres the pic. u got the white and the darker liquid layer. i guess these were not at field compacity.. ne ways the liquid i put in my chamber and inoculated one jar of organice rye berries and one jar of sterilized water and honey ... what do u think.
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santaclausmush
Stranger


Registered: 02/25/08
Posts: 263
Last seen: 10 years, 11 months
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Re: Pf tek done right [Re: Shea25]
#12422273 - 04/19/10 06:33 PM (13 years, 9 months ago) |
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these 2 jars look good but they were inoculated with LC that i used on 5 jars of wbs. the five jars of wbs formed something called cobweb mold? so alot of people are saying.
shea do u think if that the LC was bad the it would affect these two brf jars even tho the jars are turning white?
cause the wbs and five organic rye berries are turning to cobweb mold and two of the had mold ontop of the jars.   this is the organic rye berries.
these are the wbs.
 so tell me what u thing doc t. had me throw away my hpoo trays i made he said those were crap. and ur the pf tek guy so thanks for ur opinion and help. the ROOKIE.
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prismism



Registered: 05/11/09
Posts: 5,570
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those jars are very strange looking. and your rye jars didn't have cobweb.
-------------------- ephemeral anomalous
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santaclausmush
Stranger


Registered: 02/25/08
Posts: 263
Last seen: 10 years, 11 months
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ok shea i got a question?
 the yellow cap contain is burma spores in somethings i bought from spore works with the spores.
the yellow capped one i spray sterilized water in and took out the spores and made the lc and the two outside inoculated jars.
so the yellow cap is colonized. the LC is colonized. and the two outside jars im questioning.
do they look normal growth of myc? does it look ok and normal to u shea?
reason why im asking is this all my other projects have all come out this way and all have been contamed but they smell minty.. i know i said that before. so i made test brf jars and sure enough i can smell the minty smell coming out of the top of the jars threw the layer of verm.
now i have one more of the same type of spores from the same place and im gonna do it again and just want to make sure it looks ok before i go and do the same thing again. thanks for ur help shea.
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Shea25
Just some guy



Registered: 01/27/09
Posts: 7,772
Loc: Westcoast Canada
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Quote:
santaclausmush said: ok shea i got a question?
 the yellow cap contain is burma spores in somethings i bought from spore works with the spores.
the yellow capped one i spray sterilized water in and took out the spores and made the lc and the two outside inoculated jars.
so the yellow cap is colonized. the LC is colonized. and the two outside jars im questioning.
do they look normal growth of myc? does it look ok and normal to u shea?
reason why im asking is this all my other projects have all come out this way and all have been contamed but they smell minty.. i know i said that before. so i made test brf jars and sure enough i can smell the minty smell coming out of the top of the jars threw the layer of verm.
now i have one more of the same type of spores from the same place and im gonna do it again and just want to make sure it looks ok before i go and do the same thing again. thanks for ur help shea.
So what your asking me is. The 2 jars colonizing, which were inoculated with an LC made from the jar with the yellow lid are they good??.
They look alright. Are they 1/2 pint jars tho they look like pint jars
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ThirtyCigarettes
This you should vote me.


Registered: 04/20/07
Posts: 3,584
Loc: Oregon
Last seen: 8 months, 13 days
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Re: Pf tek done right [Re: Shea25]
#12575621 - 05/16/10 09:02 PM (13 years, 8 months ago) |
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Quote:
Shea25 said:

The chamber does not look like that now I changed it a round a bit no more rock in the bottom just more perlite and water
I was hoping to ask, do you have the perlite in five separate bins there? I can't quite tell. How much water is in there. I trust what you do with PF Tek, and want to give my cakes as close to the same conditions as possible.
Edited by ThirtyCigarettes (05/16/10 09:28 PM)
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spyrelx
Stranger
Registered: 05/09/10
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Re: Pf tek done right TEK [Re: Shea25]
#12707667 - 06/08/10 07:57 AM (13 years, 7 months ago) |
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So, I'm weeks into this and have got 12 jars that look like this (thanks all). In a few days I'm going to birth, dunk and roll, and place them all in a shotgun terrarium with perlite at the bottom.
I have, of course, another dumb question (not the first and I'm sure won't be the last). Its about misting and fanning. I found this quote in somebody's post on this site:
"For misting, use a clean spray bottle with a spray nozzle that produces a really fin mist. Spray above your terrarium allow mist to fall inside then fan for 10-20 seconds with the lid of your FC. Fanning out your terrarium introduces fresh air which is a key pinning trigger, removes stale air which contaminants thrive in and reduces c02. Misting helps keep your humidity up. You dont have to worry about your cakes getting a bit wet. they like to be moist. Fanning after misting will dry any large droplets of water that may have formed which is a bad thing on pins and fruitbody's."
Does that quote seem correct to you all? My real question here involves the last part, about water on the actual pins. I assume that when I get pins and I mist that they will get wet. I would also imagine that them getting wet would pretty much mimic a state of nature, so I assume I shouldn't be concerned about ANY wetness of the pins. The quote seems to say just worry about LARGE DROPS of water on the pins but not about anything else. Is that correct? Another way to ask this is how worried do I need to be about getting pins (or mushrooms themselves) wet by misting?
Regarding fanning, I assume I'm not doing it to "dry off and evaporate" the water I just misted but really just to circulate air -- is that correct?
By the way, I recognize how compulsive this all sounds ("dude, just mist and fan, OK?") but the cakes are going good, I've never done this before and I don't want to blow it at the fruiting stage.
Thanks
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spyrelx
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Re: Pf tek done right [Re: aamon]
#12719839 - 06/10/10 10:16 AM (13 years, 7 months ago) |
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Hello again,
I've birthed my cakes and they're sitting in water in the fridge (it's pretty hot here).
They all look good and I don't think this what I'm about to describe is a problem but I wanted to double check. Three of my cakes (the ones with PE spores) have one or two slight patches of yellow, like this (on the right):

And one of them has a patch of very light blue, like this (on the right):

and up top:

I'm thinking that this is fine but just wanted to double check. All three are soaking in water separated from the other cakes.
Your prognosis welcome. Thanks.
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