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InvisibleMrcanada
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Registered: 10/14/02
Posts: 63
Loc: uhmmm
Inocculating middle of jars?
    #1155781 - 12/19/02 04:23 PM (21 years, 2 months ago)

I was wondering if it would have any effect on growth of mycelium if u made an injection of spores in the middle of the jar, as well as around the sides. Would this have a considerable effect on growth?


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OfflineSkikid16
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Registered: 06/27/02
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Re: Inocculating middle of jars? [Re: Mrcanada]
    #1155787 - 12/19/02 04:25 PM (21 years, 2 months ago)

Of couse it would, more inoculation points=more spores(if you used the same per inoculation point)=faster colonization


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InvisibleSixCee
keep rolling
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Registered: 06/12/02
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Re: Inocculating middle of jars? [Re: Skikid16]
    #1155794 - 12/19/02 04:27 PM (21 years, 2 months ago)

Full colonization time is a lot less than using the usual four innoc. points.


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InvisibleMrcanada
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Re: Inocculating middle of jars? [Re: Skikid16]
    #1155798 - 12/19/02 04:28 PM (21 years, 2 months ago)

so it is true, but how much does it cut off the total amount of colonizing time? i have 8 innoculation points in my jars and i make super concentrated spore syringes and use about 1 syringe for 3 jars. these colonized the outside of the cake in about 7 days. the only part that i have to wait for it the colonization of the middle. i have lots of syringes so it doesnt matter to me how many inoculation sites it has. if it makes them grow considerably faster then everyone should do it :smile:


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InvisiblePenroc3
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Registered: 11/03/02
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Re: Inocculating middle of jars? [Re: SixCee]
    #1155804 - 12/19/02 04:29 PM (21 years, 2 months ago)

so are you saying that if you use the same amount of spores but put them on more spots on your cake it would go faster??? thats madness PURE madness(jk) i have no idea what im talking about


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InvisibleSixCee
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Re: Inocculating middle of jars? [Re: Penroc3]
    #1155809 - 12/19/02 04:31 PM (21 years, 2 months ago)

That is exactly true but there are exceptions. There are always exceptions.


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->Quote of the Moment :
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InvisibleMrcanada
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Registered: 10/14/02
Posts: 63
Loc: uhmmm
Re: Inocculating middle of jars? [Re: Penroc3]
    #1155810 - 12/19/02 04:31 PM (21 years, 2 months ago)

i read that it only takes 2 spores to start mycelium growth. if u have millions of spores, then it would be theoretically possible to make millions (let alone 10 or so) innoculation points. hmmmz


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InvisibleSixCee
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Re: Inocculating middle of jars? [Re: Mrcanada]
    #1155820 - 12/19/02 04:34 PM (21 years, 2 months ago)

Hehehe that is like saying you only need one sperm to fertilize a female egg...


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-->The above statements may or not be true.
->Quote of the Moment :
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----> PMs checked daily.


Edited by SixCee (12/19/02 04:34 PM)


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InvisibleMrcanada
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Registered: 10/14/02
Posts: 63
Loc: uhmmm
Re: Inocculating middle of jars? [Re: SixCee]
    #1155827 - 12/19/02 04:35 PM (21 years, 2 months ago)

yea, ur right, many prolly die along the way, but im sure a couple hundred thousand in 10 or so innoculation points would make a diff ?


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InvisibleSixCee
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Re: Inocculating middle of jars? [Re: Mrcanada]
    #1155836 - 12/19/02 04:37 PM (21 years, 2 months ago)

If you manage to make hundreds of innoculation points in substrate without exposing it to airborne contams you could have full colonization in less than a week! But I dunno how this coulb be done successfully.


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-->The above statements may or not be true.
->Quote of the Moment :
"Yea. All bitches are whores who love sex." -Cubie
----> PMs checked daily.


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OfflineSlighter
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Registered: 10/24/02
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Last seen: 20 years, 8 months
Post deleted by Administrator [Re: Mrcanada]
    #1155878 - 12/19/02 04:50 PM (21 years, 2 months ago)



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InvisibleMrcanada
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Registered: 10/14/02
Posts: 63
Loc: uhmmm
Re: Inocculating middle of jars? [Re: Slighter]
    #1155884 - 12/19/02 04:51 PM (21 years, 2 months ago)

makes sense  :grin: but if u would making casings out of them, u would be able to see the contams


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Offlinelivingsublime
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Registered: 10/26/02
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Re: Inocculating middle of jars? [Re: SixCee]
    #1155893 - 12/19/02 04:55 PM (21 years, 2 months ago)

How would innoculating in 10-20 different spot be any quicker then just innoculating in one spot and mixing the substrate up realy good to divide all the spores around in the substrate?


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InvisibleMrcanada
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Registered: 10/14/02
Posts: 63
Loc: uhmmm
Re: Inocculating middle of jars? [Re: livingsublime]
    #1155896 - 12/19/02 04:56 PM (21 years, 2 months ago)

that works too, most people use rye and shit, shake it up to mix it around, but my jars are packed and i cant shake them well


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