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InvisibleDoc_T
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Traveling Spore syringe - Ps. tampanensis (was Research grant syringe)
    #11534691 - 11/26/09 10:19 PM (14 years, 4 months ago)

I got a tampanensis syringe from reputable source :wink: and it's surplus for me. So I'll pass it along to somebody else.
(I'll pay postage worldwide, you have to be in a place where I can legally mail it.)

Tell us what you'll do with it. Best idea wins. Contest will run until entries stop or an obvious winner emerges or I just pick one.
Edit- contest over, the fun is just beginning.


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You make it all possible. Doesn't it feel good?

Edited by Doc_T (11/29/09 12:48 PM)

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OfflineShaggy Shaman
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Re: Research grant syringe - Ps. tampanensis [Re: Doc_T]
    #11534708 - 11/26/09 10:23 PM (14 years, 4 months ago)

Heh, I'll noc up some jars for the sclerotia, then try and fruit the substrate with a couple of sclerotia.  Then post the results here. Not fancy, but maybe the "best" thing I could do... :rockon:


--------------------
"Disbelief in magic can force a poor soul into believing in government and business."
— Tom Robbins

“The true value of a human being can be found in the degree to which he has attained liberation from the self"
-- Albert Einstein

Edited by Shaggy Shaman (11/26/09 10:25 PM)

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Offlinechad_shrooms
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Re: Research grant syringe - Ps. tampanensis [Re: Shaggy Shaman]
    #11534740 - 11/26/09 10:27 PM (14 years, 4 months ago)

Ill make up an LC, then go ahead and shoot up some jars fruit some awesome mushrooms and eat the amazing things.


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Invisiblenachohippie
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Re: Research grant syringe - Ps. tampanensis [Re: chad_shrooms]
    #11534752 - 11/26/09 10:29 PM (14 years, 4 months ago)

id make a lc and pass the luv:piss:


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send guns, money ,lawyers, and drugs its been a long night

everything i post is a lie im a pathological liar

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Invisiblenoobieshroomie
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Re: Research grant syringe - Ps. tampanensis [Re: chad_shrooms]
    #11534759 - 11/26/09 10:29 PM (14 years, 4 months ago)

since i dont grow actives ill trade it for something ill use:lol:
nice to see ya giving back to the community Doc:thumbup:

    -noobie-


--------------------
AMU

Best Thread Ever
CapZilla said:
not sure what GE and FAE are but i should probably get some.

Citric said:
Your signature is wrong on colonization temps!

GOOD JUDGMENT COMES FROM EXPERIENCE
EXPERIENCE COMES FROM BAD JUDGMENT

ROOM TEMP 70-75 IS BEST FOR COLONIZATION
Thank you mycochef

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InvisibleArtieFartie
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Re: Research grant syringe - Ps. tampanensis [Re: chad_shrooms]
    #11534765 - 11/26/09 10:31 PM (14 years, 4 months ago)

I will expand my knowledge and technique with various Psilocybin containing mushrooms, other then P. Cubensis.

Sclerotia would be nice. :laugh:

Thanks for the opportunity.


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Edited by ArtieFartie (11/26/09 10:43 PM)

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InvisibleDoc_T
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Re: Research grant syringe - Ps. tampanensis [Re: noobieshroomie]
    #11534778 - 11/26/09 10:33 PM (14 years, 4 months ago)

Yeah, print giveaways are fun but a PITA, I don't do that often. Other than that, it's pretty much about passing on knowledge more then stuff.

But I have a tampanensis already, and this one really is surplus. And I'm working with ATL 7 for a while and couldn't get to this one even if I wanted to.


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You make it all possible. Doesn't it feel good?

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InvisibleDanKnugget
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Re: Research grant syringe - Ps. tampanensis [Re: Doc_T]
    #11535023 - 11/26/09 11:11 PM (14 years, 4 months ago)

i have a deer skull that i wanna grow in, but i just don't think these would work... back to the drawing board.


--------------------
My 3 proverbs:
1. School is for people who don't know stuff.
2. Jobs are for losers.
3. Reading is for people with nothing better to do.

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InvisibleDoc_T
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Re: Research grant syringe - Ps. tampanensis [Re: DanKnugget]
    #11535955 - 11/27/09 06:16 AM (14 years, 4 months ago)

So, 'grow them' is the best answer? No experimentation? Alternate media? Fruition strategies?


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You make it all possible. Doesn't it feel good?

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OfflineWimy
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Re: Research grant syringe - Ps. tampanensis [Re: Doc_T]
    #11535990 - 11/27/09 06:37 AM (14 years, 4 months ago)

Is tampanensis a sclerotia producing species also?


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InvisibleDoc_T
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Re: Research grant syringe - Ps. tampanensis [Re: Wimy]
    #11535999 - 11/27/09 06:41 AM (14 years, 4 months ago)

Yes, one of the classics. It is mentioned in TMC.


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You make it all possible. Doesn't it feel good?

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OfflineWimy
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Re: Research grant syringe - Ps. tampanensis [Re: Wimy]
    #11536001 - 11/27/09 06:42 AM (14 years, 4 months ago)

Ahhhh im dying for sclerotia. honestly what i would do is spawn to four pint jars of millet and coffee, harvest the sclesrotia and make chocolates, and then spawn to coir/cornmeal soil and try to get some fruits! i could grow it in a cool container and take pictures lol

i dont know any advanced techniques


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Invisiblebadman
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Re: Research grant syringe - Ps. tampanensis [Re: Wimy]
    #11536031 - 11/27/09 07:00 AM (14 years, 4 months ago)

Ok here goes....

Well first off ill noc up 2 jars 400 ml of RGS and let them do their thing. When ones 100% case and fruit keep the other one stoning. Then shoot up two 20 ml MS LC and two plates. Then send the syringe to some one else when i know got clean stock.
When the time is right clone the best shroom and heaviest stone and isolate both. Test all isolates for productivity on a small scale using lil 100 ml pyrex beaker. Find the best isos store and grow stones and shrooms then dish out prints.

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Re: Research grant syringe - Ps. tampanensis [Re: badman]
    #11536286 - 11/27/09 09:01 AM (14 years, 4 months ago)

i would make a glass display case for it with all my books on how to grow mushrooms attached so i could pass it on to the next jedi. eather on here or just a random friend. who ever would use it. shit would look like the holy grail.

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Offlinefig
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Re: Research grant syringe - Ps. tampanensis [Re: fig]
    #11536298 - 11/27/09 09:04 AM (14 years, 4 months ago)

or i could let my imagination grow stones then fruit them and make syringes so my imagination could  give lots of these back to the shroomery....eather way spread the spores

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InvisibleCH HELL
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Re: Research grant syringe - Ps. tampanensis [Re: fig]
    #11536321 - 11/27/09 09:11 AM (14 years, 4 months ago)

Good to see Doc giving back.
I could go on about what I would do with it but I don't think Doc would see it.
Also if it is a true Tampanesis it is a great syringe,  Tampan is one of the originals stone producers.
CH

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Re: Research grant syringe - Ps. tampanensis [Re: CH HELL]
    #11536333 - 11/27/09 09:16 AM (14 years, 4 months ago)

tampanensis has only been found 2 times in the wild correct me if im wrong then was domesticated by growers.

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InvisibleCH HELL
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Re: Research grant syringe - Ps. tampanensis [Re: fig]
    #11536338 - 11/27/09 09:18 AM (14 years, 4 months ago)

Quote:

fig said:
tampanensis has only been found 2 times in the wild correct me if im wrong then was domesticated by growers.




I believe you are correct but it my have only been once and that was by accident.

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Offlinefig
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Re: Research grant syringe - Ps. tampanensis [Re: fig]
    #11536356 - 11/27/09 09:22 AM (14 years, 4 months ago)

i found it on wiki just now so we were both right.

Psilocybe tampanensis is a very rare psychedelic mushroom whose only known natural specimen was collected in the wild by Steven Pollock, near Tampa, Florida in 1977. All spores and cultivations of this species nowadays are originated from this only specimen. It has never again been seen in Florida but a second sighting was reported from Mississippi, however no culturable material was obtained. Psilocybe tampanensis forms psychoactive sclerotia that are known under the nickname Philosopher's stone.

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Re: Research grant syringe - Ps. tampanensis [Re: fig]
    #11536368 - 11/27/09 09:24 AM (14 years, 4 months ago)

one shroom to rule them all  dun dun duuuuu  :muahaha:

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InvisibleCH HELL
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Re: Research grant syringe - Ps. tampanensis [Re: fig]
    #11536471 - 11/27/09 09:52 AM (14 years, 4 months ago)

Quote:

fig said:
one shroom to rule them all  dun dun duuuuu  :muahaha:


:strokebeard2::evil:

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Invisiblenitelife
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Re: Research grant syringe - Ps. tampanensis [Re: CH HELL]
    #11536506 - 11/27/09 10:05 AM (14 years, 4 months ago)

First, in sterile conditions, I would seperate the contents of the syringe into 2 5cc syringes, leaving 1-2ccs in the 10cc syringe for me. I would study differences in the spores between tampanensis, other sclerotia forming psilocybes, and cubensis. After careful scientific observation, I would go give the 2 syringes to 2 of my buddies. They would be doing a scientific study. One of them would grow the tampanensis a few quart jars of wbs, rye, or grass seed (as a traditional method, or a CONTROL)- and fruit them in a realistic huge terrarium, with grass, small plants, and nutricious things for the fungi, and the other friend would try to grow the sclerotia on a method never tried before, or a failed tek.
If you guys want, I can specify some new teks that could be tried!

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Offlinedman_232
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Re: Research grant syringe - Ps. tampanensis [Re: nitelife]
    #11536532 - 11/27/09 10:16 AM (14 years, 4 months ago)

I have always wanted to try to produce a Sclerotia producer and cube hybrid... hmmm perhaps putting to agar in a dish along with something along the lines of PE isolate the combo to fresh agar repeat till stable growth and use to inoculate some rye... might have to do several 8-10 dishes to be improve odds.. could be an interesting hybrid project. has any one ever done anything like this??


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Spirals of pain pass my brain sending delicate signals of death I ignite the candle I can not handle the light within my head I grab my gun my death is done could you pull the trigger!!!

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OfflineShaggy Shaman
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Re: Research grant syringe - Ps. tampanensis [Re: Doc_T]
    #11536577 - 11/27/09 10:30 AM (14 years, 4 months ago)

Well shoot, Doc.  The sclerotia forming species are such an unknown to most of us, just trying to grow them IS an experiment for us.  I just shot up a GLC jar with some Mex-A LC to see if I can because the LC isn't developing strongly.  Trying to see if it will grow better on the grain then make a GLC to noc up 6 quarts that have been on standby for over a week. My plan is to get ATL7 as well and play with them both to study the differences they require.  Having the tampenensis as well would give me an eventual total of 3 sclerotia forming types to comparitively grow and test.  I'll be honest though and say the tamp wasn't a priority, the ATL7 is.  I already have a trade set up for a ATL7 print, IF my PE pulls through and I get some swabs.  Worst case scenario, I buy an ATL7 syringe.  This is an over-all, multi-variety study of sclerotia formers, that I plan.  The tamp would add a third variety to this.  So, nothing fancy like I said earlier but it could be a part of an experiment larger than itself.
:strokebeard:


--------------------
"Disbelief in magic can force a poor soul into believing in government and business."
— Tom Robbins

“The true value of a human being can be found in the degree to which he has attained liberation from the self"
-- Albert Einstein

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OfflineGintoniK
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Re: Research grant syringe - Ps. tampanensis [Re: Shaggy Shaman]
    #11536612 - 11/27/09 10:38 AM (14 years, 4 months ago)

i say give it to someone that you know he is going to do the what you are actually asking . Like some one with alot of ratings , journals , grow logs , proper equipment , and scientific knowledge . not a noob (like me and like others that claim pro).


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Peace and Prosperity

[

Multi-spore luck & Agar addiction

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Invisiblenitelife
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Re: Research grant syringe - Ps. tampanensis [Re: GintoniK]
    #11536639 - 11/27/09 10:47 AM (14 years, 4 months ago)

I would be a sort of Ideal person to win this..
I never won anything on here before..
Whoever you give it out to- Doc- make the right choice!

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Re: Research grant syringe - Ps. tampanensis [Re: nitelife]
    #11536659 - 11/27/09 10:56 AM (14 years, 4 months ago)

yea i do think stone producers should be spread around more it should be the first mushroom anyone trys to grow because of them not needing as much attention.

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InvisibleDanKnugget
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Re: Research grant syringe - Ps. tampanensis [Re: fig]
    #11537038 - 11/27/09 12:24 PM (14 years, 4 months ago)

I think whoever wins should try and fruit them and give away more spores... this is not me, cause i don't have any experience with sclerotia, and am busy with other projects.  Good luck to you all:thumbup:


--------------------
My 3 proverbs:
1. School is for people who don't know stuff.
2. Jobs are for losers.
3. Reading is for people with nothing better to do.

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Invisiblestonesun
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Re: Research grant syringe - Ps. tampanensis [Re: CH HELL]
    #11537193 - 11/27/09 12:57 PM (14 years, 4 months ago)

Quote:

CH HELL said:
I could go on about what I would do with it but I don't think Doc would see it.





Why wouldn't he see it?
Very nice gesture from Doc, BTW.

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InvisibleCH HELL
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Re: Research grant syringe - Ps. tampanensis [Re: stonesun]
    #11537200 - 11/27/09 12:59 PM (14 years, 4 months ago)

Quote:

stonesun said:
Quote:

CH HELL said:
I could go on about what I would do with it but I don't think Doc would see it.





Why wouldn't he see it?
Very nice gesture from Doc, BTW.





I believe he has me on ignore...LOL

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Invisiblestonesun
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Re: Research grant syringe - Ps. tampanensis [Re: CH HELL]
    #11537214 - 11/27/09 01:01 PM (14 years, 4 months ago)

As of now you're not ignored by anyone...
And you are not ignoring anyone:thumbup:

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InvisibleCH HELL
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Re: Research grant syringe - Ps. tampanensis [Re: stonesun]
    #11537266 - 11/27/09 01:10 PM (14 years, 4 months ago)

Quote:

stonesun said:
As of now you're not ignored by anyone...
And you are not ignoring anyone:thumbup:





Wow you are correct.  Doc T much have changed his mind.:cool:

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Invisiblestonesun
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Re: Research grant syringe - Ps. tampanensis [Re: CH HELL]
    #11537342 - 11/27/09 01:22 PM (14 years, 4 months ago)

Common sense won, LOL!!!
I know it's :offtopic: but
should I keep my digital hygrometer
or should I toss it?!:rofl2:


Back on topic: good luck to every candidate!
P. Tampanensis is a very interesting strain
to study.
Tough decision for Doc.

Edited by stonesun (11/27/09 01:39 PM)

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InvisibleCH HELL
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Re: Research grant syringe - Ps. tampanensis [Re: CH HELL]
    #11537352 - 11/27/09 01:24 PM (14 years, 4 months ago)

Well first thing this would do is complete my stone producers collection I have all the others.  I love to experiment so I would first take a look at the spores to see the microscopic differences between the rest of the stone producers.  Then I would have to knock up some rye jars and make a master slant to save the genetics and also some agar plates. After I have a good iso on agar I would make a large lc and experiment with pf style poo cakes to get some different effects and hopefully some mushroom body and poop stones lol,  the poop stones will be the spawn for a larger grow with coir and additives and a thin casing layer to get some mad fruits so I can spread these spores to everyone.
CH

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Offlinebw86
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Re: Research grant syringe - Ps. tampanensis [Re: CH HELL]
    #11537476 - 11/27/09 01:52 PM (14 years, 4 months ago)

Quote:

CH HELL said:
Well first thing this would do is complete my stone producers collection I have all the others.



you wish...
you mean you have all the ones that are in regular circulation. there are a shit tun of stone formers.
i wish i had the others too...
but i do have Jalsico, A and B strain,Samuiensis, Atl#7 and Tamp

Edited by bw86 (11/27/09 02:10 PM)

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OfflineWimy
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Re: Research grant syringe - Ps. tampanensis [Re: stonesun]
    #11537528 - 11/27/09 01:59 PM (14 years, 4 months ago)

how is this a tough decision im obviously the best candidate:rofl2:

Quote:

stonesun said:
Why wouldn't he see it?
Very nice gesture from Doc, BTW.




Doc IS a nice guy. And on the shroomery they say, Docs small heart grew THREE sizes that day:rofl::stoned:


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InvisibleEpilson Lyrae
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Re: Research grant syringe - Ps. tampanensis [Re: bw86]
    #11537565 - 11/27/09 02:05 PM (14 years, 4 months ago)

So far from reading the posts, I think that a clear winner has emerged.

If I won it I would put on a pretty pink skirt with a fake fur coat and hula hoop down main street on one roller skate while blasting some classic Bee Gees out of a boom box.
But I was going to do that anyway.
So.:shrug:



:shoosh:



Just kidding. Good luck to the winner though.


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"Freedom is something that dies unless it's used." H.T.
I've come to believe that the heart is the filter of the enlightened mind. Epilson Lyrae

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OfflineWimy
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Re: Research grant syringe - Ps. tampanensis [Re: Epilson Lyrae]
    #11537590 - 11/27/09 02:10 PM (14 years, 4 months ago)

i have tons of rgs but i thought millet was better for sclerotia?


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Offlinebw86
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Re: Research grant syringe - Ps. tampanensis [Re: Wimy]
    #11537611 - 11/27/09 02:13 PM (14 years, 4 months ago)

check out this post from 7 years ago (dont bump it )
http://www.shroomery.org/forums/showflat.php/Number/934289#934289

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OfflineThyrax
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Re: Research grant syringe - Ps. tampanensis [Re: bw86]
    #11537638 - 11/27/09 02:17 PM (14 years, 4 months ago)

well if you give me the syringue I would obviously try to fruit it. Never worked with sclerotia producer so I it would be a nice opportunitie to learn, can't realy experiment something since I have no knowlage anout them so I need to folow a tek to the T. And I will print as much as I can for a give away.


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InvisibleCH HELL
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Re: Research grant syringe - Ps. tampanensis [Re: bw86]
    #11537877 - 11/27/09 02:51 PM (14 years, 4 months ago)

Quote:

bw86 said:
Quote:

CH HELL said:
Well first thing this would do is complete my stone producers collection I have all the others.



you wish...
you mean you have all the ones that are in regular circulation. there are a shit tun of stone formers.
i wish i had the others too...
but i do have Jalsico, A and B strain,Samuiensis, Atl#7 and Tamp





Well yes but Tamp is the last one I am seeking...

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Offlinebw86
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Re: Research grant syringe - Ps. tampanensis [Re: CH HELL]
    #11538209 - 11/27/09 03:47 PM (14 years, 4 months ago)

why would you not want the others if you could get em?

edit- i just wanted to point out the reason i have most of them is because I SUPPORT OUR SPONSORS. its not all about trading, i bought all of the stone formers they had.

Edited by bw86 (11/27/09 03:49 PM)

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Re: Research grant syringe - Ps. tampanensis [Re: Wimy]
    #11539514 - 11/27/09 07:49 PM (14 years, 4 months ago)

Quote:

weiliiinmyyard said:
Doc IS a nice guy. And on the shroomery they say, Docs small heart grew THREE sizes that day:rofl::stoned:




Y'all should know me in real life.If you saw me at work, you'd know what a softie I am.

CH_Hell, what's your theory on poop? Are the Tampa fields poop fields? I think the original was found in the 50's, it could be city now.


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Re: Research grant syringe - Ps. tampanensis [Re: Doc_T]
    #11539648 - 11/27/09 08:27 PM (14 years, 4 months ago)

Quote:

Are the Tampa fields poop fields?




Having spent 35 years living in Florida, I can tell you this.  Magic mushrooms are directly associated with cows in the minds of the people.  Also, Florida is a top beef producing state.  I've heard that there are more cows in Florida than in Texas, but haven't verified this. The southern part of the state's agriculture is a lot of orange cultivation, while the cow country starts at the middle (just south of Orlando) of the state and goes north.  All this leads me to think that the fields were probably cow pasturage or at least old cow pasturage.  That part of the state gets a little too cold for the orange growers' comfort.  But really, only the person who found the original specimen would know.  As for those fields being swallowed by the growing city of Tampa... that too is very possible.  It is fully possible that this variety no longer survives in the wild.  Regardless of who gets this syringe, I would still like to obtain some spores of this variety just to be a part of keeping it alive.


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Re: Research grant syringe - Ps. tampanensis [Re: Doc_T]
    #11539668 - 11/27/09 08:31 PM (14 years, 4 months ago)

Quote:

Doc_T said:
Quote:

weiliiinmyyard said:
Doc IS a nice guy. And on the shroomery they say, Docs small heart grew THREE sizes that day:rofl::stoned:




Y'all should know me in real life.If you saw me at work, you'd know what a softie I am.

CH_Hell, what's your theory on poop? Are the Tampa fields poop fields? I think the original was found in the 50's, it could be city now.





No, It is just an experiment.  I believe the original tamp was found in sandy soil,  but from my experiments I have found that stone producers grow fruit bodies in close to the same conditions as pan cyans.  The poop cake would be inoculated with lc and probably produce more stones than fruit bodies,  but stones off of poo is really only good for inoculating more sub and the sclerotia fruits much easier than casing grain.
As for them being found in poo fields I doubt it,  in 1977 Pollock and his friend where at a Mycology conference and got bored and decided to take a look around and magic happen "poof" there's the Tampanensis.
CH

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Re: Research grant syringe - Ps. tampanensis [Re: Shaggy Shaman]
    #11539698 - 11/27/09 08:40 PM (14 years, 4 months ago)

Ding! We have a winner! What about a traveling syringe?

I'll attach a sterile transfer device, draw off a mil or less, then ship it to you. With the transfer device on it.
Then you draw off a bit, and send it to nitelife, who repeats and sends to weilii, etc.
Preference given to those with some cultivation experience, but no sclerotia experience. But save a bit for CH_Hell to complete his collection and compare it with the others for verification.
(You think it's some other already-known species under a different name? Or what? I don't quite get what you are saying.)

Then everybody takes their sample, does whatever,and posts it in a grow log, with links back from this thread.

We'll post here where it is and where it's going. So don't accept it if you can't go through with passing it on.

Sound good? Anybody see any obvious flaws in this plan?


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Re: Research grant syringe - Ps. tampanensis [Re: Doc_T]
    #11539900 - 11/27/09 09:30 PM (14 years, 4 months ago)

I love this idea!  It gets spread to a lot of people this way, and 1 mil is all any of us really need.  A few drops in a LC and poof plenty to play with!  Great idea Doc. I have no problem passing it on to the next person.  You need to be in charge of the order of people, though, since it is your giveaway.  Then everyone PM's their address to the person above them on the list. Excellent. I'm not experienced with a transfer device, though.  You may need to instruct us on how to get our 1 ml each :lol:


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Re: Research grant syringe - Ps. tampanensis [Re: Shaggy Shaman]
    #11539914 - 11/27/09 09:36 PM (14 years, 4 months ago)

spread the luv :piss: perfect plan


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Re: Research grant syringe - Ps. tampanensis [Re: Shaggy Shaman]
    #11539939 - 11/27/09 09:43 PM (14 years, 4 months ago)

Quote:

Shaggy Shaman said:
You need to be in charge of the order of people, though, since it is your giveaway.



I was thinking I wouldn't have to, since it's public. We'll pick each name in turn. If somebody drops out or loses interest, the chain isn't broken.
When you are ready to ship, you post to see who is ready to receive, and it goes from there. Right at first, everybody is bunched up but it'll spread out.


Quote:

Shaggy Shaman said:
I'm not experienced with a transfer device, though.  You may need to instruct us on how to get our 1 ml each :lol:




Lets you attach two Luer Lok syringes nose to nose. Then you tip it up with the full one on top and the empty on the bottom, and fill your syringe.
The portion of liquid is called an aliquot when you do it like this, BTW. Add it to the list of words you'll never use.

The thing has a cap on it, it just screws off like a syringe cap. The whole device comes off like a cap too, so CH_Hell can attach a needle to the original syringe and get every drop.
It'll make sense when you see it- but I'll post a pic here tomorrow too.


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Re: Research grant syringe - Ps. tampanensis [Re: Doc_T]
    #11539986 - 11/27/09 09:54 PM (14 years, 4 months ago)

Should we have our syringes filled with sterile water?  Is the syringe thick enough with spores that the 1 ml extracted would even out nicely in a syringe with 9 ml water in it or would that thin it out too much?  I've read that some syringes can be expanded this way.  I just ordered some more syringes from the works so I would be able to transfer and re-ship within a week or so.

Edit: Where can I get one of these transfer devices?


--------------------
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Edited by Shaggy Shaman (11/27/09 09:57 PM)

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Re: Research grant syringe - Ps. tampanensis [Re: Shaggy Shaman]
    #11539992 - 11/27/09 09:57 PM (14 years, 4 months ago)

The sterile water thing is a good idea. There's visible spores, stretching it out 1:4 or 1:9 is reasonable. Saves messing around with a drop at a time.


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Re: Research grant syringe - Ps. tampanensis [Re: Doc_T]
    #11540023 - 11/27/09 10:05 PM (14 years, 4 months ago)

Ok then.  Seems we have a plan.  By the way, where can I obtain one of these transfer devices?


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Traveling syringe - Ps. tampanensis [Re: Shaggy Shaman]
    #11540044 - 11/27/09 10:11 PM (14 years, 4 months ago)

Well, not from my source. :wink: Honestly, I'm not sure. Seems like you could buy them, but I haven't ever seen them.


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Edited by Doc_T (11/27/09 10:18 PM)

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Re: Traveling syringe - Ps. tampanensis [Re: Doc_T]
    #11540721 - 11/28/09 12:42 AM (14 years, 4 months ago)

Great idea!


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Re: Traveling syringe - Ps. tampanensis [Re: DanKnugget]
    #11540757 - 11/28/09 12:57 AM (14 years, 4 months ago)

Sounds like a plan! it would be easiest if i could just send ch_hell a 2_ml syringe and he can mail it to one of my friends that will take care of me..........................


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Re: Traveling syringe - Ps. tampanensis [Re: Wimy]
    #11541407 - 11/28/09 07:22 AM (14 years, 4 months ago)

Quote:

weiliiinmyyard said:
Sounds like a plan! it would be easiest if i could just send ch_hell a 2_ml syringe and he can mail it to one of my friends that will take care of me..........................




Details like this can be worked out among the various players.
But this does point out that a Google Maps app with the syringe route is probably not a good idea.


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Re: Research grant syringe - Ps. tampanensis [Re: bw86]
    #11541447 - 11/28/09 07:42 AM (14 years, 4 months ago)

Quote:

bw86 said:
check out this post from 7 years ago (dont bump it )
http://www.shroomery.org/forums/showflat.php/Number/934289#934289




Bumped.


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Edited by dancefloordale (11/28/09 07:49 AM)

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Re: Traveling syringe - Ps. tampanensis [Re: Doc_T]
    #11541737 - 11/28/09 09:23 AM (14 years, 4 months ago)

well hell yeah then. :handth:


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Re: Research grant syringe - Ps. tampanensis [Re: Shaggy Shaman]
    #11541739 - 11/28/09 09:24 AM (14 years, 4 months ago)

Quote:

Shaggy Shaman said:
  You may need to instruct us on how to get our 1 ml each :lol:




Done. How to use a Sterile Transfer Device.
This shows me drawing into a 1 mL, but you can use a 10, or whatever you like. If there's sterile water in your syringe, DO NOT shoot it into the master unit.


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Edited by Doc_T (11/28/09 09:33 AM)

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Re: Research grant syringe - Ps. tampanensis [Re: Doc_T]
    #11541860 - 11/28/09 09:52 AM (14 years, 4 months ago)

Excellent tutorial, Doc.  What size is the smaller syringe?  Is the amount you took what you wish us to take as well, or do you want us to take less? I'm not sure how many people this syringe is traveling to.

Edit: :facepalm: doh, just read where you said its a 1 ml... nvm


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Edited by Shaggy Shaman (11/28/09 09:53 AM)

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Re: Research grant syringe - Ps. tampanensis [Re: Shaggy Shaman]
    #11541890 - 11/28/09 09:57 AM (14 years, 4 months ago)

I used a 1 mL and took one full milliliter. Take as much as you need but as little as you can.
So, maybe use a 10 mL, draw off 0.5 to 1.0 mL, then expand your syringe with sterile water.

If somebody is going to expand the master syringe, I'd like it to be somebody more experienced (no offense).
But that could easily be done at least once, maybe twice. So this could visit anywhere from 10 to possibly 40 or 50 people.

Obviously,it's going to get contaminated somewhere along the line... treat it as a suspect source until proven otherwise.

Edit- it doesn't have to travel in a line, it can branch.
So you could send off the original, expand your syringe, and split it three ways to share. For example.


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Edited by Doc_T (11/28/09 10:01 AM)

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Re: Research grant syringe - Ps. tampanensis [Re: Doc_T]
    #11542491 - 11/28/09 11:55 AM (14 years, 4 months ago)

Quote:

Obviously,it's going to get contaminated somewhere along the line...




The master syringe shouldn't get contaminated as long as no one pushes into it, right?  The contam issue shouldn't be a worry until after someone expands the master I would think.


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Re: Research grant syringe - Ps. tampanensis [Re: Shaggy Shaman]
    #11542548 - 11/28/09 12:09 PM (14 years, 4 months ago)

if nobody gets careless we will be fine.

Im gonna expand this syringe tonight. any pointers?


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Edited by weiliiinmyyard (11/28/09 12:16 PM)

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Re: Research grant syringe - Ps. tampanensis [Re: Shaggy Shaman]
    #11542640 - 11/28/09 12:30 PM (14 years, 4 months ago)

Quote:

Shaggy Shaman said:
Quote:

Obviously,it's going to get contaminated somewhere along the line...




The master syringe shouldn't get contaminated as long as no one pushes into it, right?  The contam issue shouldn't be a worry until after someone expands the master I would think.




Exactly. But every time it's handled there's a chance. It's like rolling dice again and again- you'll get snake eyes sooner or later.

The package is on its way to you and should arrive soon. Post here when you get it, please. :smile:

Edit-
Here's a map link. Should let anybody edit it to add themselves.

Edited by Doc_T (12/01/09 09:45 AM)

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Re: Research grant syringe - Ps. tampanensis [Re: Doc_T]
    #11548598 - 11/29/09 12:35 PM (14 years, 4 months ago)

Looks like we all will win off this,  thanks Doc.
My internet was down so if I missed anything let me know.
CH

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Re: Research grant syringe - Ps. tampanensis [Re: CH HELL]
    #11548643 - 11/29/09 12:43 PM (14 years, 4 months ago)

Nothing new in the thread- spores are in the mail to Shaggy Shaman.
I made a grow log for my spores, I shot two tenths of a mil of spore water into a pint jar of Thick 'n' Chunky LC.
I hope everybody will post something about what they end up doing.


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Edited by Doc_T (11/29/09 12:50 PM)

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Re: Research grant syringe - Ps. tampanensis [Re: Doc_T]
    #11556378 - 11/30/09 04:26 PM (14 years, 4 months ago)

Ok, I have received the syringe and extracted 1 ml in a still air box.  I then put 5 drops into a 50 ml honey LC. Who is ready for it now?
:strokebeard:


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Re: Research grant syringe - Ps. tampanensis [Re: Shaggy Shaman]
    #11556459 - 11/30/09 04:37 PM (14 years, 4 months ago)

If youre offering...

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Re: Research grant syringe - Ps. tampanensis [Re: badman]
    #11556539 - 11/30/09 04:47 PM (14 years, 4 months ago)

badman would prb be a good candidate to split the syringe atleast 2 ways. whoevers gonna go next should split the syringe


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Re: Research grant syringe - Ps. tampanensis [Re: Wimy]
    #11556687 - 11/30/09 05:07 PM (14 years, 4 months ago)

I am in no way experienced enough to ask to get in the line up for this syringe. However I would just like to say that this is an awesome idea and such an awesome strain to do it with. I can't wait to see the stones you guys come up with. Best of luck to all who are lucky enough to get the syringe. :thumbup:


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Re: Research grant syringe - Ps. tampanensis [Re: Moonraker]
    #11556748 - 11/30/09 05:14 PM (14 years, 4 months ago)

Ill split it however you want just let me know if I'm privileged/worthy enough for this honor.

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Re: Research grant syringe - Ps. tampanensis [Re: badman]
    #11556816 - 11/30/09 05:22 PM (14 years, 4 months ago)

oooohhh i just saw the london part. ehh:shrug:

aint up to me


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Re: Research grant syringe - Ps. tampanensis [Re: Wimy]
    #11557124 - 11/30/09 06:05 PM (14 years, 4 months ago)

whos choice is it??

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Re: Research grant syringe - Ps. tampanensis [Re: badman]
    #11557318 - 11/30/09 06:29 PM (14 years, 4 months ago)

its great to see how this comunity is willing to spread the love im not advanced enough yet to ask to get the syringe when all of thecapable people isee inline are going todo some good but im sure glad it will be going to the knowlageble folks here good idea doc:whoo:


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Re: Research grant syringe - Ps. tampanensis [Re: nachohippie]
    #11557360 - 11/30/09 06:34 PM (14 years, 4 months ago)

If you want to send it to me I will separate it into 1ml syringes or vacutainer,  then refill the syringe so it can go a few more rounds.
CH

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Re: Research grant syringe - Ps. tampanensis [Re: CH HELL]
    #11557465 - 11/30/09 06:51 PM (14 years, 4 months ago)

Quote:

CH HELL said:
If you want to send it to me I will separate it into 1ml syringes or vacutainer,  then refill the syringe so it can go a few more rounds.
CH




Are you saying you want to split it into 1 ml syringes and then mail them each out to all the others CH?

Moonraker and Nachohippie:  Doc specified that priority was for it to actually go to those with no sclerotia experience.  Check up-thread for his intentions with starting this.

I think the idea of one experienced person splitting it then sending 1 ml syringes out is a good one.  Less chance of contams if the right person does it.  The prohibiting factor is all the postage.  In a line we all only have to pay to send to the next, whoever splits will have to mail 5-8 envelopes out from their own pocket.  If everyone likes the idea of CH doing this I'm all for it.


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Re: Research grant syringe - Ps. tampanensis [Re: Shaggy Shaman]
    #11557523 - 11/30/09 07:00 PM (14 years, 4 months ago)

Moonraker and Nachohippie:  Doc specified that priority was for it to actually go to those with no sclerotia experience.  Check up-thread for his intentions with starting this.


well if thats the priority id love to give it a try pm me for addy if there is still any left :shrug:


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Re: Research grant syringe - Ps. tampanensis [Re: nachohippie]
    #11557692 - 11/30/09 07:25 PM (14 years, 4 months ago)

i would also be more than happy to give this an attempt. i have not grown stones b4, but i would love to try something new! i will do a grow log and everything if one gets sent my way

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Re: Research grant syringe - Ps. tampanensis [Re: ShroomHunting]
    #11557744 - 11/30/09 07:34 PM (14 years, 4 months ago)

hell id even breakdown and buy a camera to do a log  :picdidnthappen:
LOL


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Re: Research grant syringe - Ps. tampanensis [Re: badman]
    #11557811 - 11/30/09 07:44 PM (14 years, 4 months ago)

Quote:

badman said:
whos choice is it??





I'd like to step in here.

I think it's best to reduce the number of border crossings, but it's fine to travel within a country.
Do you already have other contacts there?
If so, you could expand a single mil out to be a few samples.

I was thinking the same about Canada, actually, I wondered if Truskool was going to get in on it.

Quote:

Shaggy Shaman said:
  If everyone likes the idea of CH doing this I'm all for it.



I didn't what to do it that way. I don't mind a few branches, but the idea was traveling spores.

Edit- in my mind, I was seeing this working like the free spore print thread in the marketplace.
When it's ready to move, then the next person claims it and you send it on.
Like I said, I don't mind a few branches along the way, but my original idea was that the syringe would travel.

But I'm not locked into that. Send it back to me, I'll split it up.
I wish somebody had said something sooner instead of waiting until AFTER THE FACT, but I guess that how it is when you deal with stoners. :crankey:
I'll PM you my address.

Edited by Doc_T (11/30/09 08:04 PM)

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Re: Research grant syringe - Ps. tampanensis [Re: Doc_T]
    #11558121 - 11/30/09 08:31 PM (14 years, 4 months ago)

Forget what I said it doesn't matter to me, I was trying to be nice but I guess it didn't work.  I will shut my internet mouth.
CH
P.s.  I have hundreds of 1ml syringes and could separate the and then send the entire package of syringes to the next person.  This would cut down on the contam rate 10 fold.  A transfer device should only be used once and then considered contaminated unless sterilized properly.

Edited by CH HELL (11/30/09 08:36 PM)

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Re: Research grant syringe - Ps. tampanensis [Re: CH HELL]
    #11558130 - 11/30/09 08:33 PM (14 years, 4 months ago)

Wait, wait. Check PMs.


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Re: Research grant syringe - Ps. tampanensis [Re: CH HELL]
    #11558273 - 11/30/09 08:53 PM (14 years, 4 months ago)

Quote:

CH HELL said:
I have hundreds of 1ml syringes




Well, then send them around the world if you like.
People, do whatever you want. I'm done- it's out of my hands.


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Re: Research grant syringe - Ps. tampanensis [Re: Doc_T]
    #11558332 - 11/30/09 09:06 PM (14 years, 4 months ago)

Quote:

Doc_T said:
Quote:

CH HELL said:
I have hundreds of 1ml syringes




Well, then send them around the world if you like.
People, do whatever you want. I'm done- it's out of my hands.





This is your deal Doc I didn't mean to step on any toes.

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Re: Research grant syringe - Ps. tampanensis [Re: Doc_T]
    #11558367 - 11/30/09 09:12 PM (14 years, 4 months ago)

It would be a shame for this to end badly or with hard feelings. This reminded me of the traveling gnome stolen from someone's yard, I believe in CA. The "thieves" took it with them on vacation somewhere and "the gnome" sent a postcard home to the original owner. The gnome was then handed off to another traveler who also sent the obligatory postcard home. This continued for a long while as the gnome traveled the world over before finally being reunited with it's owner and was made famous.

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Re: Research grant syringe - Ps. tampanensis [Re: SporeTek]
    #11558385 - 11/30/09 09:14 PM (14 years, 4 months ago)

gnomes have all the luck


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Re: Research grant syringe - Ps. tampanensis [Re: nachohippie]
    #11558577 - 11/30/09 09:38 PM (14 years, 4 months ago)

Quote:

nachohippie said:
gnomes have all the luck





Would you steal someone with a smelly soundin' name like nachohippie and treat them to a world vacation?  :FattyNoneck:

99% of all fungal spores say no. :eek:

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Re: Research grant syringe - Ps. tampanensis [Re: SporeTek]
    #11558661 - 11/30/09 09:46 PM (14 years, 4 months ago)

i wouldnt fit in the overhead compartment


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Re: Research grant syringe - Ps. tampanensis [Re: Doc_T]
    #11559020 - 11/30/09 10:26 PM (14 years, 4 months ago)

Awww, don't be like that Doc.  We all still love you.  :sunny:
What do you expect from kids with a new toy? :lmafo:

For the rest of you guys, Doc and CH worked out a separate exchange, so we are now back on to a traveling syringe.  So, I would like to mail this to someone in the continental U.S. and to someone with at least some practical experience so there is less chance for contamination. I think after the 3rd ml is taken it may be a good time to do a little expansion of the main syringe, so it will not be too thinned down.  Lets do the nominate and second thing and talk about this.  I don't want to randomly pick the next person.


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Re: Research grant syringe - Ps. tampanensis [Re: Shaggy Shaman]
    #11560511 - 12/01/09 04:52 AM (14 years, 4 months ago)

Yeh Doc i got a few contacts in the UK but its OK. I sourced some mex B, jal, col, prints so i got stone spores to work with, i wanna see if they are genuine. Good luck with the pike syringe guys and remember to spray with IPA. In fact if you have two IPA sprayers you can use one of them to sparay the other one :thumbup:

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Re: Research grant syringe - Ps. tampanensis [Re: Shaggy Shaman]
    #11560518 - 12/01/09 04:56 AM (14 years, 4 months ago)

Yeh Doc i got a few contacts in the UK but its OK. I sourced some mex B, jal, col, prints so i got stone spores to work with, i wanna see if they are genuine. Good luck with the pike syringe guys and remember to spray with IPA. In fact if you have two IPA sprayers you can use one of them to sparay the other one :thumbup:

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Re: Research grant syringe - Ps. tampanensis [Re: Shaggy Shaman]
    #11560683 - 12/01/09 06:52 AM (14 years, 4 months ago)

Quote:

SporeTek said:
Quote:

nachohippie said:
gnomes have all the luck





Would you steal someone with a smelly soundin' name like nachohippie and treat them to a world vacation?  :FattyNoneck:

99% of all fungal spores say no. :eek:





HAHHAHAHAHAHAHAAAAAAAAAA
Quote:

Shaggy Shaman said:
Awww, don't be like that Doc.  We all still love you.  :sunny:
What do you expect from kids with a new toy? :lmafo:

For the rest of you guys, Doc and CH worked out a separate exchange, so we are now back on to a traveling syringe.  So, I would like to mail this to someone in the continental U.S. and to someone with at least some practical experience so there is less chance for contamination. I think after the 3rd ml is taken it may be a good time to do a little expansion of the main syringe, so it will not be too thinned down.  Lets do the nominate and second thing and talk about this.  I don't want to randomly pick the next person.





I'll take it shag. pm. but-

Quote:

Doc_T said:
I'll attach a sterile transfer device, draw off a mil or less, then ship it to you. With the transfer device on it.
Then you draw off a bit, and send it to nitelife, who repeats and sends to weilii, etc.
Preference given to those with some cultivation experience, but no sclerotia experience.




-nitelife would be next according.

Quote:

Doc_T said:
Send it back to me, I'll split it up.
I wish somebody had said something sooner instead of waiting until AFTER THE FACT, but I guess that how it is when you deal with stoners. :crankey:
I'll PM you my address.





my fault, bad idea. i deffinatly had my eyes on the prize and lost sight of what you are trying to accomplish here. i just want to grow some sclerotia!:tongue:


--------------------

Edited by weiliiinmyyard (12/01/09 07:16 AM)

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Re: Research grant syringe - Ps. tampanensis [Re: Wimy]
    #11560835 - 12/01/09 07:52 AM (14 years, 4 months ago)

Quote:

-nitelife would be next according.




Doc also said to post and see who was ready for it.  I think the reference to nitelife being next was an example given, not a definite order. He hasn't posted as being ready either, and counting my false start :smile: he hasn't spoken up in 2 calls for the next in line.  I'm gonna send it to Weilli.


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Re: Research grant syringe - Ps. tampanensis [Re: Shaggy Shaman]
    #11560850 - 12/01/09 07:58 AM (14 years, 4 months ago)

Quote:

Shaggy Shaman said:
he hasn't spoken up in 2 calls for the next in line.  I'm gonna send it to Weilli.




Sounds good to me.

Let me clarify my earlier posts: I don't think anybody broke this idea, I think it broke itself while Shaggy and CH_Hell happened to be there.
In other words, it's all good. :thumbup:

Shaggy Shaman, are you going to do a grow log? What do you have planned for these?


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Re: Research grant syringe - Ps. tampanensis [Re: Doc_T]
    #11560959 - 12/01/09 08:43 AM (14 years, 4 months ago)

cool.

I'm gonna knock up 1 jar and do g2g transfers. Don't know too much about live cultures but I expect I will in an hour or so. Maybe I'll do that

doc- I expanded that b+ syringe we were talking about. easy peesy. I used a ziplock baggy instead of a shotglass.

depending on who it's going to next, i could expand it.

how many cc syringe and how many cc's left, shag?


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Re: Research grant syringe - Ps. tampanensis [Re: Doc_T]
    #11560963 - 12/01/09 08:44 AM (14 years, 4 months ago)

Quote:

Shaggy Shaman, are you going to do a grow log? What do you have planned for these?




I've never done a grow log before, but sure, it could be my first!  Am I supposed to create it all at once, or post along and along as things are done?  If the latter, I can go ahead and show my baby LC!  As for my plans, I have 6 quart jars prepped already for sclerotia growing. These will mostly be used for my Mexicana A LC which is really taking off since I diluted it with some sterile water.  Apparently they don't like a 4% honey LC.  The same day I received the traveler, I had prepped 3 LC's around 3% and used one for 5 drops of the tamp and one of the others for some Oak Ridge.  I figure at least a couple of weeks before the tamp LC is ready, then I will noc up some jars.  What kinda sucks, is that I found out last night that I need to move.  I'm thinking of heading for california, but am waiting for things to become clear.  On the positive side, sclerotia jars should be very easy to move and maintain.  Also, since I am a new owner of a sterile transfer device :smile: I have contacted the fellow I was gonna trade a PE swab to for his ATL 7 print.  I am offering him a 2 ml syringe of PE which he should get more use out of than a swab.  If he likes the idea, it allows me to go ahead and do the trade now before I move.  This would give me 3 sclerotia lines to play with.  So, right now, waiting on my PE tub and waiting on LC's. :lmafo:


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Re: Research grant syringe - Ps. tampanensis [Re: Wimy]
    #11560973 - 12/01/09 08:47 AM (14 years, 4 months ago)

Quote:

how many cc syringe and how many cc's left, shag?




Its a 12 ml vendor syringe.  Doc took 1 ml i think and I took 1 ml.  It has the label on it and the plunger is still behind it, so kinda hard to tell exactly what line it sits on.  But I would say 10 ml left.


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Re: Research grant syringe - Ps. tampanensis [Re: Shaggy Shaman]
    #11561000 - 12/01/09 08:55 AM (14 years, 4 months ago)

weli im ready i have a agar petri dishw aiting if its avaliable :shrug:i have a atl#7 stone on agar would love the tamp toadd to my "stones of the world" colection pm me and we can keep this stone rollin :grin:


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Re: Research grant syringe - Ps. tampanensis [Re: nachohippie]
    #11561058 - 12/01/09 09:11 AM (14 years, 4 months ago)

hey i need a minute to think about this. you already have sclerotia


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Edited by weiliiinmyyard (12/01/09 09:12 AM)

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Re: Research grant syringe - Ps. tampanensis [Re: Wimy]
    #11561089 - 12/01/09 09:18 AM (14 years, 4 months ago)

Quote:

weiliiinmyyard said:
hey i need a minute to think about this. you already have sclerotia




I can't make rules, it's out of my hands. But I can make suggestions, and I'd suggest that you can't call it in advance.
Like the Free Print thread in the Marketplace, right? You wait until it comes up, then you post your claim.


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Re: Research grant syringe - Ps. tampanensis [Re: Doc_T]
    #11561111 - 12/01/09 09:22 AM (14 years, 4 months ago)

Its totally NOT out of your hands. i'd much rather you tell me exactly what to do lol.


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Re: Research grant syringe - Ps. tampanensis [Re: Wimy]
    #11561127 - 12/01/09 09:26 AM (14 years, 4 months ago)

if this is only to people with no scrolieria not a problem i just get excited thinking about my new hobby this shit is kinda taking over my life ... but in a good way :awedrugs:


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Re: Research grant syringe - Ps. tampanensis [Re: nachohippie]
    #11561133 - 12/01/09 09:28 AM (14 years, 4 months ago)

doc you are the guy who has given us all this oppertunity even if i dont get a chance thanx for spreading the luv ur the man!!


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Re: Research grant syringe - Ps. tampanensis [Re: nachohippie]
    #11561141 - 12/01/09 09:30 AM (14 years, 4 months ago)

No, no- open to anybody.
But I would hope those with prior sclerotia experience would try something new. Or do the same old thing in a side-by-side grow.
Something more advanced than 'grow some stones in a jar'.
So nachohipipe or anybody, if you are on when/if it comes up, claim it.
Weilii, you can't promise something you don't have- so don't post the request until you are ready to send it on.

Also, whenever it gets expanded, that's the opportunity to split it in half and send part overseas.


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Re: Research grant syringe - Ps. tampanensis [Re: Doc_T]
    #11561181 - 12/01/09 09:40 AM (14 years, 4 months ago)

thanks to bw86 i kinda have my heart set on the fruits now. anyway sclerotia form on top of the bulk sub right?

thats my plan.


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Re: Research grant syringe - Ps. tampanensis [Re: Doc_T]
    #11561184 - 12/01/09 09:41 AM (14 years, 4 months ago)

the atl#7 is my first attempt it is only a stone on agar i havent grown stones b4 so this would be a first time side by side grow i would deffinatly do a grow log im even gonna buy a camera [ wifes gona get a camera from me for a bday present ha ha ] and doing a agar dish to get experience in agar im a newbie i only so far have all thisstuff in theroy it would be my first real life experiance hopefully today iwill beknockingupmyfirst cakes :awedrugs:


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Re: Research grant syringe - Ps. tampanensis [Re: nachohippie]
    #11561225 - 12/01/09 09:48 AM (14 years, 4 months ago)

Give it to nacho!!! :w0ahhhhmahnnn:

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Re: Research grant syringe - Ps. tampanensis [Re: nachohippie]
    #11561235 - 12/01/09 09:49 AM (14 years, 4 months ago)

My ATL cakes have given three fruits so far, and they all looked printable. I didn't get visible prints though, I gotta try again.


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Re: Research grant syringe - Ps. tampanensis [Re: stonesun]
    #11561244 - 12/01/09 09:51 AM (14 years, 4 months ago)

wow sun thanx for the endorsement you are 2 good 2 me id give you  another rating if i could thanx  :toast:


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Re: Research grant syringe - Ps. tampanensis [Re: Doc_T]
    #11561248 - 12/01/09 09:52 AM (14 years, 4 months ago)

Quote:

nachohippie said:
the atl#7 is my first attempt it is only a stone on agar i havent grown stones b4 so this would be a first time side by side grow i would deffinatly do a grow log im even gonna buy a camera [ wifes gona get a camera from me for a bday present ha ha ] and doing a agar dish to get experience in agar im a newbie i only so far have all thisstuff in theroy it would be my first real life experiance hopefully today iwill beknockingupmyfirst cakes :awedrugs:




take some time, breathe, and use the space bar.:lol:

is that the guy that is inside shorty's head smoking weed in scary movie 3. haha i think it deffinatly is.

Quote:

Doc_T said:
My ATL cakes have given three fruits so far, and they all looked printable. I didn't get visible prints though, I gotta try again.




did you take pics? gimme some porn yo.


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Re: Research grant syringe - Ps. tampanensis [Re: nachohippie]
    #11561264 - 12/01/09 09:54 AM (14 years, 4 months ago)

i cant wait till i have prints to give all you guys everyone here has been soo cool helping me get ready for this new obsesion of mine im making my first still air box friday when i get paid cant wait im so glad i found this site since ihad to stop growing my medecin i really needed a new hobby cheers to you all


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Re: Research grant syringe - Ps. tampanensis [Re: Wimy]
    #11561276 - 12/01/09 09:57 AM (14 years, 4 months ago)

Quote:

weiliiinmyyard said:
Quote:

Doc_T said:
My ATL cakes have given three fruits so far, and they all looked printable. I didn't get visible prints though, I gotta try again.




did you take pics? gimme some porn yo.






All three opened up, but I guess I didn't let them open far enough.


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Re: Research grant syringe - Ps. tampanensis [Re: Doc_T]
    #11561309 - 12/01/09 10:02 AM (14 years, 4 months ago)

very cool. reminds me of zapotecorum.
http://www.shroomery.org/forums/showflat.php/Number/586123#586123
ignore the first pics of the wild specimens.. and, see the indoor ones have upturned caps when they are mature

I wonder how tall they get

did the stipes have similar texture also? I don't know the correct term

this is cool-
Psilocybe tampanensis is a very rare psychedelic mushroom whose only known natural specimen was collected in the wild by Steven Pollock, near Tampa, Florida in 1977. All spores and cultivations of this species nowadays are originated from this only specimen. It has never again been seen in Florida but a second sighting was reported from Mississippi, however no culturable material was obtained.- Wikipedia


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Edited by weiliiinmyyard (12/01/09 10:11 AM)

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Re: Research grant syringe - Ps. tampanensis [Re: Wimy]
    #11561356 - 12/01/09 10:11 AM (14 years, 4 months ago)

Let's continue this in that thread, since it's a whole different species.
http://www.shroomery.org/forums/showflat.php/Number/11492658

As for this-
Quote:

weiliiinmyyard said:
this is cool-
Psilocybe tampanensis is a very rare psychedelic mushroom whose only known natural specimen was collected in the wild by Steven Pollock, near Tampa, Florida in 1977. All spores and cultivations of this species nowadays are originated from this only specimen. It has never again been seen in Florida but a second sighting was reported from Mississippi, however no culturable material was obtained.- Wikipedia



CH_Hell was saying something upthread about this, how it sounds fishy.
Anyone have any theories?


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Edited by Doc_T (12/01/09 10:55 AM)

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Re: Research grant syringe - Ps. tampanensis [Re: Wimy]
    #11561682 - 12/01/09 11:06 AM (14 years, 4 months ago)

Quote:

anyway sclerotia form on top of the bulk sub right?




The sclerotia form in the substrate. Some report that after spawning and being cased that they form more sclerotia just under the surface.  But then again, I have been researching a lot on Mexicana A and may have my facts mixed up.  I do believe tampenensis is more known for fruiting though and not making a ton of sclerotia.  I guess we are all gonna find out! 

The syringe is officially on its way!  Just posted it. Get ready Weilli.


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Re: Research grant syringe - Ps. tampanensis [Re: Shaggy Shaman]
    #11561769 - 12/01/09 11:24 AM (14 years, 4 months ago)

let the journey begin  :jailbait:


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Re: Research grant syringe - Ps. tampanensis [Re: nachohippie]
    #11561799 - 12/01/09 11:28 AM (14 years, 4 months ago)

really quick and then back on topic Weilii In My Yard lots of people call me wimy

easier to type. Wasnt being very considerate when i chose this name. :lol:

good deal cant wait!


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Re: Research grant syringe - Ps. tampanensis [Re: Wimy]
    #11561872 - 12/01/09 11:39 AM (14 years, 4 months ago)

I'd be willing to try some things out with it. Though, I'm not gonna fight anyone for it.


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Re: Research grant syringe - Ps. tampanensis [Re: dancefloordale]
    #11561904 - 12/01/09 11:42 AM (14 years, 4 months ago)

:suicidebomber:


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Re: Research grant syringe - Ps. tampanensis [Re: nachohippie]
    #11562029 - 12/01/09 11:57 AM (14 years, 4 months ago)

No fighting! :crankey:
And no suicide bombing! :kingcrankey:


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Re: Research grant syringe - Ps. tampanensis [Re: Doc_T]
    #11562068 - 12/01/09 12:02 PM (14 years, 4 months ago)

But I'm a :arabs: Suicide bombing is what I do.


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Re: Research grant syringe - Ps. tampanensis [Re: dancefloordale]
    #11562092 - 12/01/09 12:05 PM (14 years, 4 months ago)

sorry :awedrugs:


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Re: Research grant syringe - Ps. tampanensis [Re: dancefloordale]
    #11562252 - 12/01/09 12:24 PM (14 years, 4 months ago)

:arabs:

LOL! what a good emoticon

it appears nobody wants to touch that joke with a ten foot pole! :lol:


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Re: Research grant syringe - Ps. tampanensis [Re: Wimy]
    #11562271 - 12/01/09 12:26 PM (14 years, 4 months ago)

Well, I was looking for a terrorist one, but couldn't find it, so arabs had to suffice.

I laughed, that's what matters.


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Re: Research grant syringe - Ps. tampanensis [Re: dancefloordale]
    #11562286 - 12/01/09 12:28 PM (14 years, 4 months ago)

now you are gonna have to get a package sniffer to check for letter bombs lol


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Re: Research grant syringe - Ps. tampanensis [Re: nachohippie]
    #11575550 - 12/03/09 08:52 AM (14 years, 4 months ago)

where its at?:macdre:


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Re: Research grant syringe - Ps. tampanensis [Re: nachohippie]
    #11575727 - 12/03/09 09:43 AM (14 years, 4 months ago)

It's in the mail, heading for Wimy.


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Re: Research grant syringe - Ps. tampanensis [Re: Shaggy Shaman]
    #11576456 - 12/03/09 12:21 PM (14 years, 4 months ago)

and it didn't come today


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Re: Research grant syringe - Ps. tampanensis [Re: nachohippie]
    #11578634 - 12/03/09 05:56 PM (14 years, 4 months ago)

Quote:

nachohippie said:
where its at?:macdre:




Check the map, Jack.


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Re: Research grant syringe - Ps. tampanensis [Re: Doc_T]
    #11578951 - 12/03/09 06:31 PM (14 years, 4 months ago)

cool didnt realize there was a map


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Re: Research grant syringe - Ps. tampanensis [Re: nachohippie]
    #11578976 - 12/03/09 06:34 PM (14 years, 4 months ago)

It's upthread, we'll have to repost it.
You can edit the map and zoom in and out and do other stuff, then make a link to that exact map setting.
So make it any way you like when it's your turn. :thumbup:


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Re: Research grant syringe - Ps. tampanensis [Re: Doc_T]
    #11578985 - 12/03/09 06:35 PM (14 years, 4 months ago)

cool


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Re: Research grant syringe - Ps. tampanensis [Re: nachohippie]
    #11580083 - 12/03/09 09:13 PM (14 years, 4 months ago)

I can't find the rubber ring to my pc!:mad2:

it really doesnt seem to be anywhere. it could be where i knocked up jars last i guess... maybe under the bed. but not in the kitchen

if i can't find it, i'm gonna need to figure something out to keep that syringe moving.

ideas?


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Re: Research grant syringe - Ps. tampanensis [Re: Wimy]
    #11580517 - 12/03/09 10:20 PM (14 years, 4 months ago)

$10 new set of ring seal and safety plug. :shrug:


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Re: Research grant syringe - Ps. tampanensis [Re: Epilson Lyrae]
    #11580615 - 12/03/09 10:36 PM (14 years, 4 months ago)

yeah lol. or any way i could store a couple cc's until i find the one thats made for mine.


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Re: Research grant syringe - Ps. tampanensis [Re: Wimy]
    #11580629 - 12/03/09 10:38 PM (14 years, 4 months ago)

how would you store?? do you have any agar dishs

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Re: Research grant syringe - Ps. tampanensis [Re: nachohippie]
    #11580695 - 12/03/09 10:48 PM (14 years, 4 months ago)

Pre-sterilized Glass vials.


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Re: Research grant syringe - Ps. tampanensis [Re: dancefloordale]
    #11580734 - 12/03/09 10:55 PM (14 years, 4 months ago)

Quote:

dancefloordale said:
Pre-sterilized Glass vials.




where do i buy those


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Re: Research grant syringe - Ps. tampanensis [Re: Wimy]
    #11580865 - 12/03/09 11:18 PM (14 years, 4 months ago)

Idk. I got some (from a friend), but that syringe is going to reach you way before I could send any out.



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Edited by dancefloordale (12/03/09 11:21 PM)

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Re: Research grant syringe - Ps. tampanensis [Re: dancefloordale]
    #11581020 - 12/03/09 11:46 PM (14 years, 4 months ago)

yeah. well do you wanna go next and you could send me one back..


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Re: Research grant syringe - Ps. tampanensis [Re: Wimy]
    #11581060 - 12/03/09 11:54 PM (14 years, 4 months ago)

open a new syringe?


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Re: Research grant syringe - Ps. tampanensis [Re: DanKnugget]
    #11581128 - 12/04/09 12:10 AM (14 years, 4 months ago)

Quote:

weiliiinmyyard said:
yeah. well do you wanna go next and you could send me one back..




Sure, I guess. I'd have to get prepared, otherwise i'll probably just put some in a vial too.

Wouldn't it just be easier for you to find a replacement. Try Ace hardware? Idk, I'm willing but it seems like a lot of extra work and wasted time.

Just let me know.


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Re: Research grant syringe - Ps. tampanensis [Re: dancefloordale]
    #11581144 - 12/04/09 12:14 AM (14 years, 4 months ago)

what they dont have sterile vials at ace hardware that seems like something you have to order off the internet. and then that way you get to go next. if you donty wanna go next i'll figure something out. i dont even have the thing yet


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Re: Research grant syringe - Ps. tampanensis [Re: Wimy]
    #11581189 - 12/04/09 12:20 AM (14 years, 4 months ago)

Haha, no a new ring for your PC.

I just want to do the most time efficient thing so this can keep getting passed on.

How long would it take you to get a new ring? Surely quicker than me waiting to get it from you, then sending it back. No?


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Re: Research grant syringe - Ps. tampanensis [Re: dancefloordale]
    #11581234 - 12/04/09 12:30 AM (14 years, 4 months ago)

why can't you just open a new sterile syringe? is there something i'm missing?


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Re: Research grant syringe - Ps. tampanensis [Re: DanKnugget]
    #11581250 - 12/04/09 12:33 AM (14 years, 4 months ago)

Idk whats going on anymore....


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Re: Research grant syringe - Ps. tampanensis [Re: DanKnugget]
    #11581274 - 12/04/09 12:38 AM (14 years, 4 months ago)

I dont have money or new syringes. if i dont have something figured out when it gets here im gonna pass it on

i bet i can get something from my EMT friend.

what don't you get. I was gonna knock up some grains and a lc. now i cant use my pc. theres no sterile transfer device anymore. no way to preserve ss.


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Re: Research grant syringe - Ps. tampanensis [Re: Wimy]
    #11581316 - 12/04/09 12:46 AM (14 years, 4 months ago)

I'll just send you some, wait for them, then you can send it to me/someone else.

I think that will be quickest anyway. PM me.


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Re: Research grant syringe - Ps. tampanensis [Re: Wimy]
    #11582154 - 12/04/09 05:42 AM (14 years, 4 months ago)

Quote:

weiliiinmyyard said: now i cant use my pc.




Couldn't you find the ring in the morning when you are sober and have some coffee?



Quote:

weiliiinmyyard said: theres no sterile transfer device anymore. 



Goddamn better be a sterile transfer device on that syringe when you get it! :crankey:

Quote:

weiliiinmyyard said:  no way to preserve ss.



Sterile spores?


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Re: Research grant syringe - Ps. tampanensis [Re: Doc_T]
    #11582253 - 12/04/09 06:12 AM (14 years, 4 months ago)

Quote:

Doc_T said:

Quote:

weiliiinmyyard said: theres no sterile transfer device anymore. 



Goddamn better be a sterile transfer device on that syringe when you get it! :crankey:





:curbyourenthusiasm:

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Re: Research grant syringe - Ps. tampanensis [Re: badman]
    #11582259 - 12/04/09 06:15 AM (14 years, 4 months ago)

People: Leave the device on the syringe. Uncap and recap the device for transfers.
If it's your syringe, you may remove the transfer device like a cap, and attach a needle.


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Re: Research grant syringe - Ps. tampanensis [Re: Wimy]
    #11582548 - 12/04/09 08:20 AM (14 years, 4 months ago)

Quote:

weiliiinmyyard said:
I dont have money or new syringes. if i dont have something figured out when it gets here im gonna pass it on

i bet i can get something from my EMT friend.

what don't you get. I was gonna knock up some grains and a lc. now i cant use my pc. theres no sterile transfer device anymore. no way to preserve ss.




Ummm.  There is still a sterile transfer device attached to it.  I performed my extraction in near-sterile conditions in a still air box and re-capped the STD.  It is very simple people.  If you say you are ready for the syringe, you should have an empty syringe ready.  Do your clean transfer and send it on to the next person who is ready with a syringe.


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Re: Research grant syringe - Ps. tampanensis [Re: Doc_T]
    #11582571 - 12/04/09 08:30 AM (14 years, 4 months ago)

Quote:

Goddamn better be a sterile transfer device on that syringe when you get it! :crankey:




Relax, Doc.  The syringe and transfer device are like I received them from you, less 1 ml solution.  There is no need for this kind of drama.  Anyone who steps forward for the syringe at the time of the call should be ready!  This means having a sterile syringe on hand when you say "send it to me!" No one should be getting fancy and thinking they are gonna take the transfer device off of it to do anything else but a syringe to syringe transfer.  Once you make the transfer, you can do whatever you want with YOUR syringe.


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Re: Research grant syringe - Ps. tampanensis [Re: Shaggy Shaman]
    #11582592 - 12/04/09 08:35 AM (14 years, 4 months ago)

Quote:

Shaggy Shaman said: Ummm. [...] It is very simple people.




Welcome to my world. :rolleyes:

Weilii, you can remove the transfer device and attach a needle and expel a bit, if that's how you have to do it.
Store the thing inside the alc pad packet or somewhere else sterile for a few seconds while you shoot.
What you expel it into is your problem. People talk about microwave LC, maybe this is your chance to try it. :lol:

Quote:

There is no need for this kind of drama.



Purely for effect. No real drama, I promise. :laugh:



Quote:

Anyone who steps forward for the syringe at the time of the call should be ready!  This means having a sterile syringe on hand when you say "send it to me!" No one should be getting fancy and thinking they are gonna take the transfer device off of it to do anything else but a syringe to syringe transfer.  Once you make the transfer, you can do whatever you want with YOUR syringe.



YES!


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Re: Research grant syringe - Ps. tampanensis [Re: Doc_T]
    #11582614 - 12/04/09 08:42 AM (14 years, 4 months ago)

ready willing and able:lolsy:


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Re: Research grant syringe - Ps. tampanensis [Re: nachohippie]
    #11582718 - 12/04/09 09:11 AM (14 years, 4 months ago)

Welii you can make a sterile LC without a PC or sterilize an old syringe with boiling water.
CH

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Re: Research grant syringe - Ps. tampanensis [Re: CH HELL]
    #11583554 - 12/04/09 12:06 PM (14 years, 4 months ago)

I don't know what the best thing to do to keep this syringe moving along is, but I shipped him a couple sterile vials anyway, just in case. If he does decide to use one to store his portion it shouldn't delay this thing for more than a day (assuming the syringe arrives today).


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OfflineWimy
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Re: Research grant syringe - Ps. tampanensis [Re: Doc_T]
    #11583989 - 12/04/09 01:40 PM (14 years, 4 months ago)

Quote:

Doc_T said:
Quote:

weiliiinmyyard said: now i cant use my pc.




Couldn't you find the ring in the morning when you are sober and have some coffee?



Quote:

weiliiinmyyard said: theres no sterile transfer device anymore. 



Goddamn better be a sterile transfer device on that syringe when you get it! :crankey:

Quote:

weiliiinmyyard said:  no way to preserve ss.



Sterile spores?



dude i just read this you are so funny. shortly after i woke up i made coffee and found my ring.. wow.


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Re: Research grant syringe - Ps. tampanensis [Re: Shaggy Shaman]
    #11584007 - 12/04/09 01:44 PM (14 years, 4 months ago)

Quote:

Shaggy Shaman said:
Quote:

weiliiinmyyard said:
I dont have money or new syringes. if i dont have something figured out when it gets here im gonna pass it on

i bet i can get something from my EMT friend.

what don't you get. I was gonna knock up some grains and a lc. now i cant use my pc. theres no sterile transfer device anymore. no way to preserve ss.




Ummm.  There is still a sterile transfer device attached to it.  I performed my extraction in near-sterile conditions in a still air box and re-capped the STD.  It is very simple people.  If you say you are ready for the syringe, you should have an empty syringe ready.  Do your clean transfer and send it on to the next person who is ready with a syringe.




dude shut the fuuyuccckkkkk up. im pc'ing some syringes right now.
go read the pms you were very very eager to pick me.

i got it, theres a sterile transfer device, sorry you said "now im the proud owner of my very own sterile transfer device" threw me off. theres a huge bubble in the syringe and it wasnt in a baggy, so i hope that air bubble was sucked into the syringe through a needle with a flame on the other end of it or we might have some problems. so far all I have done is get it, throw it in a ziplock, and start pcing my syringes.

dale is up next

Quote:

Doc_T said:
Purely for effect. No real drama, I promise. :laugh:





:ass:


--------------------

Edited by weiliiinmyyard (12/04/09 01:58 PM)

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Re: Research grant syringe - Ps. tampanensis [Re: Wimy]
    #11584184 - 12/04/09 02:19 PM (14 years, 4 months ago)

Quote:

weiliiinmyyard said:
shortly after i woke up i made coffee and found my ring.




Usually works like that. :laugh:


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Re: Research grant syringe - Ps. tampanensis [Re: Doc_T]
    #11584268 - 12/04/09 02:38 PM (14 years, 4 months ago)

yeah haha. i jumped the gun on that one. dale you are a good guy reguardless:thumbup:

i've got like a million pm's. lol be careful what you wish for

i'll get it out tomorrow probably. keep you guys posted

Quote:

Doc_T said:

Quote:

weiliiinmyyard said:  no way to preserve ss.



Sterile spores?




spore solution. same thing


--------------------

Edited by weiliiinmyyard (12/04/09 02:51 PM)

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Re: Research grant syringe - Ps. tampanensis [Re: Wimy]
    #11584776 - 12/04/09 04:01 PM (14 years, 4 months ago)

Quote:

weiliiinmyyard said:
dude shut the fuuyuccckkkkk up. im pc'ing some syringes right now.
go read the pms you were very very eager to pick me.





You posted that you were ready for it and you were the only one to do so that has been in the thread from the beginning.  I also thought you had your shit together from my past reading of your posts in other threads.  Please take this as a compliment.  Lighten up, I'm just trying to help Doc keep this thing going smoothly.  Also, my last post kinda defines what "ready" should mean to all the other people who may consider stepping forward for this.  Please don't see this as aimed solely at you.

Quote:

i got it, theres a sterile transfer device, sorry you said "now im the proud owner of my very own sterile transfer device" threw me off. theres a huge bubble in the syringe and it wasnt in a baggy, so i hope that air bubble was sucked into the syringe through a needle with a flame on the other end of it or we might have some problems. so far all I have done is get it, throw it in a ziplock, and start pcing my syringes.




Thank god you received it, now the pressure is off of ME.  Doc threw me a transfer device to keep, I would never take the one off the syringe, that would screw the pooch for everyone else. If you read Doc's tek on using the transfer device, you will see he explains the bubble. I would suggest doing your transfer in a glove box so that if it sucks in more air it will not contaminate, maybe.  Thats what I did.
Or try pushing the master while pulling the secondary.

No hard feelings, I hope....


--------------------
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Re: Research grant syringe - Ps. tampanensis [Re: Shaggy Shaman]
    #11584891 - 12/04/09 04:24 PM (14 years, 4 months ago)

no dont take me seriously.
wimy=not to be taken seriously

im gonna use a gallon zip lock bag after i lysol and let that settle. my glovebox is a fruiting chamber right now


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Re: Research grant syringe - Ps. tampanensis [Re: Shaggy Shaman]
    #11584988 - 12/04/09 04:44 PM (14 years, 4 months ago)

Quote:

Shaggy Shaman said:
Thank god you received it, now the pressure is off of ME.  Doc threw me a transfer device to keep, I would never take the one off the syringe, that would screw the pooch for everyone else. If you read Doc's tek on using the transfer device, you will see he explains the bubble. I would suggest doing your transfer in a glove box so that if it sucks in more air it will not contaminate, maybe.  Thats what I did.
Or try pushing the master while pulling the secondary.

No hard feelings, I hope....




i still didnt read the tek yet im pretty busy but the seal inbetween the transfer device is good so if i pull an equal amount of sterile air into the recieving syringe before the transfer would it not solve the problem


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Re: Research grant syringe - Ps. tampanensis [Re: Wimy]
    #11585003 - 12/04/09 04:48 PM (14 years, 4 months ago)

What problem?


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Re: Research grant syringe - Ps. tampanensis [Re: Doc_T]
    #11585085 - 12/04/09 05:03 PM (14 years, 4 months ago)

bad air being sucked in the syringe


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Re: Research grant syringe - Ps. tampanensis [Re: Wimy]
    #11585132 - 12/04/09 05:10 PM (14 years, 4 months ago)

If that had happened, and it didn't, then it's too late now. You can't suck the contams out.


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Re: Research grant syringe - Ps. tampanensis [Re: Doc_T]
    #11585385 - 12/04/09 05:49 PM (14 years, 4 months ago)

no no no

nevermind

where is that tek i cant find it

he was saying read the tek which explains the bubble, and use my glovebox, or try pushing the pluger and pulling the donor plunger at the same time during the exchange.

so im thinking during the transfer in gonna get air in the master syringe to replace the expelled spore solution. but if it was attatched to a doner syringe with sterile air it would solve that problem, then i could get all the air into the *EDIT*donor* syringe along with a mil or so of spore solution, put on a needled, expunge the air and wipe it down. the master syringe never has to leave the gallon ziplock bag its in now. am i not getting something


--------------------

Edited by weiliiinmyyard (12/04/09 06:33 PM)

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Re: Research grant syringe - Ps. tampanensis [Re: Wimy]
    #11585495 - 12/04/09 06:12 PM (14 years, 4 months ago)

I just got the air in my room clean, got my hands clean, pulled a cooled off syringe out of the pc and tossed it right in the bag with the master syringe. sealed the mag most of the way, expelled the bit of air that got in out and sealed it tight. I had a little trouble getting the cap off of the STD, but then attatching the donor sytinge was done easitly from the outside of the bag



now without even opening the bag im about to draw off a bit.

it was easy to do without opening the bag by pulling and pusing at the same time, but its harder to do it that way so i drew of abt 1 1/2 CC instead of the 1 i was aiming for



--------------------

Edited by weiliiinmyyard (12/04/09 06:17 PM)

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Re: Research grant syringe - Ps. tampanensis [Re: Wimy]
    #11585557 - 12/04/09 06:20 PM (14 years, 4 months ago)

Wow your transfer device is way more chunky than mine. Are they PP?

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Re: Research grant syringe - Ps. tampanensis [Re: badman]
    #11585677 - 12/04/09 06:37 PM (14 years, 4 months ago)

It's got handles on the side so you don't touch it.
PP = autoclaveable? Yes, the red part is.


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Re: Research grant syringe - Ps. tampanensis [Re: Doc_T]
    #11585755 - 12/04/09 06:48 PM (14 years, 4 months ago)

Seen seen, mine have lil wings but those have to be way more convenient to use.

PP-polyprop. autoclavable, yes boss.

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Re: Research grant syringe - Ps. tampanensis [Re: badman]
    #11589679 - 12/05/09 12:06 PM (14 years, 4 months ago)

Got mine,  Thanks Doc.

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Re: Research grant syringe - Ps. tampanensis [Re: CH HELL]
    #11591052 - 12/05/09 03:56 PM (14 years, 4 months ago)

My tamp LC has gone all cloudy.  The other LC solution I made at the same time is clear.  Has anyone else tried using the tamp solution, yet?  The master may be contaminated...

Edit: After UTSF, I'm gonna use 5 more drops of Tamp MS into another LC that I have on hand.  The contam may have been in the baby food jar, and now I know bacterial endospores can survive 15 min @ 15 psi.  I'll let you guys know what happens.


--------------------
"Disbelief in magic can force a poor soul into believing in government and business."
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Edited by Shaggy Shaman (12/05/09 04:06 PM)

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Re: Research grant syringe - Ps. tampanensis [Re: Shaggy Shaman]
    #11592331 - 12/05/09 07:26 PM (14 years, 4 months ago)

If your LC is cloudy to start with, that's fine. It the ones that start clear and then cloud up that are a problem.
Really, you can't tell much by looking. So unless it starts to fizz, let it colonize for 2-3 weeks and see what you get.


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Re: Research grant syringe - Ps. tampanensis [Re: Doc_T]
    #11593134 - 12/05/09 09:37 PM (14 years, 4 months ago)

It started out clear with a little sediment.  Yesterday it was REAL cloudy.  I had used a baby food jar obtained from my neighbors.  Its already been disposed of, and 8 drops of spore solution noc'ed into a half pint jar of pre-prepared LC that was on standby and sitting waiting.  This one has been sitting ready for a week or so and is clear so I know the LC is clean, eliminating it as a suspect.  If this one clouds, its either the solution or my inoculation technique. :strokebeard:


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Re: Research grant syringe - Ps. tampanensis [Re: Shaggy Shaman]
    #11593168 - 12/05/09 09:46 PM (14 years, 4 months ago)

it needed to be in a plastic baggy.i cleaned the exterior of the syringe but it could have been too late


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Re: Research grant syringe - Ps. tampanensis [Re: Wimy]
    #11593318 - 12/05/09 10:23 PM (14 years, 4 months ago)

I'm whipping up some agar now. If it is contaminated, we will know soon.


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Everything posted by the user dancefloordale, aside from what is written in this here clause is completely and utterly fictitious, despite any information read (or seen), above (or below) that might lead you to believe otherwise.

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Re: Research grant syringe - Ps. tampanensis [Re: dancefloordale]
    #11593359 - 12/05/09 10:33 PM (14 years, 4 months ago)

:thumbup: getting it out monday pretty sure

but its not deffinate. i have to get a ride and nobody else really cares about my hobby


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Re: Research grant syringe - Ps. tampanensis [Re: Wimy]
    #11599816 - 12/06/09 10:22 PM (14 years, 4 months ago)

Quote:

weiliiinmyyard said:
:thumbup: getting it out monday pretty sure




Hey guys, while using the search function, it seems because we are actually sending a physical object to another person, we are all due to get trade ratings.  You rate whoever mails the syringe to you.  This is kinda cool because we are doing one long chain not just a 2 person deal.  In the rating comments we should make a note of the traveling syringe somehow.  I would rate Doc but can't rate yet :frown:


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Re: Research grant syringe - Ps. tampanensis [Re: Shaggy Shaman]
    #11599924 - 12/06/09 10:40 PM (14 years, 4 months ago)

:thumbup:

im seriously gonna hit up the maillady for 5 in stamps

i wonder if she will do it

does anybody know if you can buy them anywhere else? like the grocery store?

you can do alot of things at the grocery store these days.


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Re: Research grant syringe - Ps. tampanensis [Re: Shaggy Shaman]
    #11599937 - 12/06/09 10:42 PM (14 years, 4 months ago)

Quote:

Shaggy Shaman said:
In the rating comments we should make a note of the traveling syringe somehow. 




Well, there is the map.
If you don't want your actual location on it, you could use a fictional one.
:strokebeard: Hm... Vegas, baby! Edit- or Tijuana. Or a million other places.

@weilii: 7-11 and other similar stores.


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Re: Research grant syringe - Ps. tampanensis [Re: Wimy]
    #11601913 - 12/07/09 09:25 AM (14 years, 4 months ago)

Thanks for the rating Wimy!

Doc - I'll rate you in February when I qualify, muahahahaha.


--------------------
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Re: Research grant syringe - Ps. tampanensis [Re: Doc_T]
    #11602216 - 12/07/09 10:24 AM (14 years, 4 months ago)

Quote:

Doc_T said:
@weilii: 7-11 and other similar stores.


:thumbup:

and the ATM apparently


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Re: Research grant syringe - Ps. tampanensis [Re: Wimy]
    #11603309 - 12/07/09 01:18 PM (14 years, 4 months ago)

Quote:

weiliiinmyyard said:
Quote:

Doc_T said:
@weilii: 7-11 and other similar stores.


:thumbup:

and the ATM apparently




Shit, I thought I mentioned that. :lol: I was going to but apparently I didn't. Oh well, you figured it out on your own.


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Re: Research grant syringe - Ps. tampanensis [Re: dancefloordale]
    #11603569 - 12/07/09 01:49 PM (14 years, 4 months ago)

dam this thing has not moved in days lol

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Re: Research grant syringe - Ps. tampanensis [Re: fig]
    #11603662 - 12/07/09 02:00 PM (14 years, 4 months ago)

i didnt get it outb today but my brother left the house an hour ago with 5 bucks i gave him assuring he will return with stamps. so if i dont get it out tomorrow then you can really let me have it.

oh and i got the vials dale. even if they don't heal they are sterile on the inside so i'll put em to good use! :thumbup: thanks


--------------------

Edited by weiliiinmyyard (12/07/09 02:02 PM)

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Re: Research grant syringe - Ps. tampanensis [Re: Wimy]
    #11603780 - 12/07/09 02:15 PM (14 years, 4 months ago)

really is snailmail lol:lolsy:


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Re: Research grant syringe - Ps. tampanensis [Re: nachohippie]
    #11603844 - 12/07/09 02:23 PM (14 years, 4 months ago)

when did i even get it? i tried sat. sunday no mail. couldnt work it out soon enough today

no wheels dude no wheeeels


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Re: Research grant syringe - Ps. tampanensis [Re: Wimy]
    #11604055 - 12/07/09 02:57 PM (14 years, 4 months ago)

I feel ya, I have no wheels either, it sometimes makes things a bit difficult.


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Re: Research grant syringe - Ps. tampanensis [Re: DanKnugget]
    #11604661 - 12/07/09 04:16 PM (14 years, 4 months ago)

yeah and they only sell the stamp books at a minimun of 9.50! :loogie:

i think i have a ride to the post office tomorrow. anyway he kept my money and said that he probably can so he probably will.

i'll keep you guys posted, i guess.


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Re: Research grant syringe - Ps. tampanensis [Re: Wimy]
    #11610000 - 12/08/09 02:20 PM (14 years, 4 months ago)

i just got 20 stamps. im sorry people i thought i had my shit together.

in the box


--------------------

Edited by weiliiinmyyard (12/08/09 08:03 PM)

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Re: Research grant syringe - Ps. tampanensis [Re: Wimy]
    #11615234 - 12/09/09 07:54 AM (14 years, 4 months ago)

Is anyone ready for some spores?
I received 1ml and turned it into three.  I have it in a vacutainer that I will mail with a few 1cc syringes.
CH

P.S.  There does seem to be some bacteria in the syringe.

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Re: Research grant syringe - Ps. tampanensis [Re: CH HELL]
    #11615381 - 12/09/09 08:29 AM (14 years, 4 months ago)

bacteria you say :minigun: id be willing to fight some stinkin bacteria
wouldnt peroxide agar do the trick??


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Re: Research grant syringe - Ps. tampanensis [Re: nachohippie]
    #11615450 - 12/09/09 08:47 AM (14 years, 4 months ago)

Quote:

nachohippie said:
bacteria you say :minigun: id be willing to fight some stinkin bacteria
wouldnt peroxide agar do the trick??





Any agar will work,  I have never used h2o2 agar and always manage to get it clean.
If you are ready I will send it your way with the rest of the stuff I owe you.
CH

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Re: Research grant syringe - Ps. tampanensis [Re: CH HELL]
    #11615455 - 12/09/09 08:48 AM (14 years, 4 months ago)

sure i need to get some agar work going anyway :scaryshroom:


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everything i post is a lie im a pathological liar

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Re: Research grant syringe - Ps. tampanensis [Re: CH HELL]
    #11615569 - 12/09/09 09:14 AM (14 years, 4 months ago)

do the needles screw on to the 1 mL syringes? if so is there anything you want for a blnk one or two or three


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Re: Research grant syringe - Ps. tampanensis [Re: Wimy]
    #11615573 - 12/09/09 09:16 AM (14 years, 4 months ago)

There's two kinds of 1mL syringes. Luer Lok and slip tip. I think most of us are using Luer connectors.


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Re: Research grant syringe - Ps. tampanensis [Re: Doc_T]
    #11615582 - 12/09/09 09:19 AM (14 years, 4 months ago)

I had some "slip-tip" 2 mL
i just need them for mailing wbu samples

btw your LA is on the way as of right now
same with the traveling tampenensis syringe it's on the way to dale

mail lady just took it


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Edited by weiliiinmyyard (12/09/09 09:21 AM)

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Re: Research grant syringe - Ps. tampanensis [Re: Wimy]
    #11615589 - 12/09/09 09:21 AM (14 years, 4 months ago)

And then there's 'oral syringes' which come in all sizes and colors, but don't have Luer fittings.


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Re: Research grant syringe - Ps. tampanensis [Re: Doc_T]
    #11615625 - 12/09/09 09:29 AM (14 years, 4 months ago)

those would suck to use. im stoked about you giving me these 40mm needles my old ones were pretty short


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Re: Research grant syringe - Ps. tampanensis [Re: Wimy]
    #11615794 - 12/09/09 10:04 AM (14 years, 4 months ago)

Quote:

weiliiinmyyard said:
do the needles screw on to the 1 mL syringes? if so is there anything you want for a blnk one or two or three





My 1ml syringes are fix tip insulin syringes,  so they would not work with the std.
CH

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Re: Research grant syringe - Ps. tampanensis [Re: CH HELL]
    #11615818 - 12/09/09 10:10 AM (14 years, 4 months ago)

right i got my two mL from a diabetic lady too. she had a whole box of them

nifty for discreet mailing


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Re: Research grant syringe - Ps. tampanensis [Re: Wimy]
    #11615856 - 12/09/09 10:19 AM (14 years, 4 months ago)

I think peroxide agar is for working with fresh tissue, the spores might have a hard time germinating on it.


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Re: Research grant syringe - Ps. tampanensis [Re: DanKnugget]
    #11630473 - 12/11/09 02:11 PM (14 years, 4 months ago)

Got the syringe today, will do my thing with it and get it out tomorrow. Though, it's still going to be slow arriving to the next person, with no Sunday mail and holiday shipping.

I just got back from the post office and that place was a hell hole. Picked up some petri's and an inoculation loop. :thumbup: Anyone seeing growth yet? Did this thing get contaminated?

So who's up next?


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Re: Research grant syringe - Ps. tampanensis [Re: dancefloordale]
    #11630489 - 12/11/09 02:14 PM (14 years, 4 months ago)

i still havent done anything with mine.

i cleaned the exterior and bagged it man. should be fine if it was.

you know,you could try tossing your empty syring in the ziplock with that one, sealing it back up, and doing it from outside the bag. it's pretty simple once you get the cap off. Thats what i did.


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Re: Research grant syringe - Ps. tampanensis [Re: Wimy]
    #11630627 - 12/11/09 02:34 PM (14 years, 4 months ago)

Sounds good. Has everyone been using the same envelope? I think i'll pick up a new one, this one is starting to look kinda rough :lol:

Now, gotta go take a big ass :glittershitz: I know, tmi.


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Re: Research grant syringe - Ps. tampanensis [Re: dancefloordale]
    #11630635 - 12/11/09 02:35 PM (14 years, 4 months ago)

actually that was a used envelope somebody else sent me


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Re: Research grant syringe - Ps. tampanensis [Re: Wimy]
    #11630690 - 12/11/09 02:44 PM (14 years, 4 months ago)

Quote:

weiliiinmyyard said:
actually that was a used envelope somebody else sent me



I reuse them.


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Re: Research grant syringe - Ps. tampanensis [Re: Doc_T]
    #11630818 - 12/11/09 03:06 PM (14 years, 4 months ago)

its starting to get suspicious with all the tape

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Re: Research grant syringe - Ps. tampanensis [Re: Wimy]
    #11630914 - 12/11/09 03:21 PM (14 years, 4 months ago)

Yeah, I'll just throw it in a new one, no biggie. 4 stamps, really? That's cool and all but damn.


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Re: Research grant syringe - Ps. tampanensis [Re: dancefloordale]
    #11630923 - 12/11/09 03:22 PM (14 years, 4 months ago)

yeah dude and it was like 1 g under two ounces after ripping some tape off


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Re: Research grant syringe - Ps. tampanensis [Re: Wimy]
    #11632129 - 12/11/09 07:04 PM (14 years, 4 months ago)

Who am I sending this to? It goes out tomorrow.


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Re: Research grant syringe - Ps. tampanensis [Re: dancefloordale]
    #11632141 - 12/11/09 07:05 PM (14 years, 4 months ago)

whyblameus wantys it
he's quick too

if it is non machinable and over 3/4 inches thick and not iniformly thick the postage price is

2 ounces $1.39


--------------------

Edited by weiliiinmyyard (12/11/09 07:11 PM)

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Re: Research grant syringe - Ps. tampanensis [Re: Wimy]
    #11632265 - 12/11/09 07:21 PM (14 years, 4 months ago)

i wanna see a pic of this mangled envelope:lol:

  -noobie-


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CapZilla said:
not sure what GE and FAE are but i should probably get some.

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Re: Research grant syringe - Ps. tampanensis [Re: noobieshroomie]
    #11632356 - 12/11/09 07:36 PM (14 years, 4 months ago)


Made up my syringe.

Also pictured is the inoculation loop I got today. Yay me. :thumbup:

No pic of the envelope, my batteries died. It's not that bad, but it looks a little abused. You can tell it's been around the block.


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Re: Research grant syringe - Ps. tampanensis [Re: Wimy]
    #11632363 - 12/11/09 07:37 PM (14 years, 4 months ago)

Quote:

weiliiinmyyard said:
whyblameus wantys it
he's quick too

if it is non machinable and over 3/4 inches thick and not iniformly thick the postage price is

2 ounces $1.39




If he wants he he better get me his info. Here that wbu?

Edit - :lol:, Also, I already have it. :facepalm:


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Edited by dancefloordale (12/11/09 07:38 PM)

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Re: Research grant syringe - Ps. tampanensis [Re: Wimy]
    #11632366 - 12/11/09 07:37 PM (14 years, 4 months ago)

Quote:

weiliiinmyyard said:
whyblameus wantys it
he's quick too

if it is non machinable and over 3/4 inches thick and not iniformly thick the postage price is

2 ounces $1.39



:biggrin:


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Re: Research grant syringe - Ps. tampanensis [Re: whyblameus]
    #11632372 - 12/11/09 07:38 PM (14 years, 4 months ago)

doing it now.:rockon:


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Re: Research grant syringe - Ps. tampanensis [Re: Wimy]
    #11632382 - 12/11/09 07:39 PM (14 years, 4 months ago)

Quote:

weiliiinmyyard said:
if it is non machinable and over 3/4 inches thick and not iniformly thick the postage price is

2 ounces $1.39




You sound like an expert!


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Re: Research grant syringe - Ps. tampanensis [Re: dancefloordale]
    #11632401 - 12/11/09 07:41 PM (14 years, 4 months ago)

Quote:

dancefloordale said:
Quote:

weiliiinmyyard said:
whyblameus wantys it
he's quick too

if it is non machinable and over 3/4 inches thick and not iniformly thick the postage price is

2 ounces $1.39




If he wants he he better get me his info. Here that wbu?

Edit - :lol:, Also, I already have it. :facepalm:



you already have it.
from what it might not be mine i use other peoples addresses but i want this to come to me.


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Re: Research grant syringe - Ps. tampanensis [Re: Doc_T]
    #11633356 - 12/11/09 10:30 PM (14 years, 4 months ago)

Quote:

Doc_T said:
Quote:

weiliiinmyyard said:
if it is non machinable and over 3/4 inches thick and not iniformly thick the postage price is

2 ounces $1.39




You sound like an expert!



an expert that can't type uniform. lol.


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Re: Research grant syringe - Ps. tampanensis [Re: Wimy]
    #11649530 - 12/14/09 03:27 PM (14 years, 4 months ago)

Guys, it went out today. I had something come up on Saturday and couldn't make it to the post office. Also, I think you will enjoy the upgraded packaging.


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Re: Research grant syringe - Ps. tampanensis [Re: dancefloordale]
    #11669153 - 12/17/09 03:13 PM (14 years, 3 months ago)

haha i have been checking the mail waiting.

i didnt see the last post.:facepalm:

:rofl2:


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Re: Research grant syringe - Ps. tampanensis [Re: whyblameus]
    #11669173 - 12/17/09 03:17 PM (14 years, 3 months ago)

:lolsy: i got 1 4 ya


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Re: Research grant syringe - Ps. tampanensis [Re: whyblameus]
    #11669452 - 12/17/09 03:56 PM (14 years, 3 months ago)

:biggrin:


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Re: Research grant syringe - Ps. tampanensis [Re: whyblameus]
    #11677583 - 12/18/09 08:39 PM (14 years, 3 months ago)

can there be live mycelium in a spore syringe? any tiny stand like substances?

if not then my syringe was contaminated before i knocked up an lc, because i could see it immediatly


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Re: Research grant syringe - Ps. tampanensis [Re: Wimy]
    #11677620 - 12/18/09 08:47 PM (14 years, 3 months ago)

in you lc?
it could be from the honey.
is it growing?


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Re: Research grant syringe - Ps. tampanensis [Re: Wimy]
    #11677623 - 12/18/09 08:48 PM (14 years, 3 months ago)

yea spores can germinate in the syringe

  -noobie-


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CapZilla said:
not sure what GE and FAE are but i should probably get some.

Citric said:
Your signature is wrong on colonization temps!

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ROOM TEMP 70-75 IS BEST FOR COLONIZATION
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Re: Research grant syringe - Ps. tampanensis [Re: whyblameus]
    #11677632 - 12/18/09 08:50 PM (14 years, 3 months ago)

not yet and yeah you are right it could be the honey but i didnt see it before. its like a whispy organism. i can even see it anymore

i have them on the windowsill in ziplocks, where i keep my spores and stuff. lol my room is 75 but the windowsill is like a refridgerator.

do they need to be incubated?


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Re: Research grant syringe - Ps. tampanensis [Re: Wimy]
    #11677645 - 12/18/09 08:51 PM (14 years, 3 months ago)

just keep it at room temp
75 is perfect

  -noobie-


--------------------
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Best Thread Ever
CapZilla said:
not sure what GE and FAE are but i should probably get some.

Citric said:
Your signature is wrong on colonization temps!

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ROOM TEMP 70-75 IS BEST FOR COLONIZATION
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Re: Research grant syringe - Ps. tampanensis [Re: noobieshroomie]
    #11677654 - 12/18/09 08:52 PM (14 years, 3 months ago)

k ill move it to the other side of the room with my jars:thumbup:


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Re: Research grant syringe - Ps. tampanensis [Re: Wimy]
    #11677659 - 12/18/09 08:54 PM (14 years, 3 months ago)

the LC right?

    -noobie-


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Best Thread Ever
CapZilla said:
not sure what GE and FAE are but i should probably get some.

Citric said:
Your signature is wrong on colonization temps!

GOOD JUDGMENT COMES FROM EXPERIENCE
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ROOM TEMP 70-75 IS BEST FOR COLONIZATION
Thank you mycochef

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Re: Research grant syringe - Ps. tampanensis [Re: whyblameus]
    #11677666 - 12/18/09 08:55 PM (14 years, 3 months ago)

i find it hard to tell the difference from myc and contam in lc.till you shoot up a test jar.


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Re: Research grant syringe - Ps. tampanensis [Re: noobieshroomie]
    #11677673 - 12/18/09 08:56 PM (14 years, 3 months ago)

Quote:

noobieshroomie said:
the LC right?

    -noobie-



yeah


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Re: Research grant syringe - Ps. tampanensis [Re: Wimy]
    #11677689 - 12/18/09 09:00 PM (14 years, 3 months ago)

Quote:

whyblameus said:
i find it hard to tell the difference from myc and contam in lc.till you shoot up a test jar.



Quote:

weiliiinmyyard said:
Quote:

noobieshroomie said:
the LC right?

    -noobie-



yeah



:thumbup:
one thing i have noticed is if your LC goes cloudy
usually its no good

  -noobie-


--------------------
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Best Thread Ever
CapZilla said:
not sure what GE and FAE are but i should probably get some.

Citric said:
Your signature is wrong on colonization temps!

GOOD JUDGMENT COMES FROM EXPERIENCE
EXPERIENCE COMES FROM BAD JUDGMENT

ROOM TEMP 70-75 IS BEST FOR COLONIZATION
Thank you mycochef

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Re: Research grant syringe - Ps. tampanensis [Re: noobieshroomie]
    #11679190 - 12/19/09 04:53 AM (14 years, 3 months ago)

Quote:

noobieshroomie said:
one thing i have noticed is if your LC goes cloudy
usually its no good

  -noobie-




Yep. It can start cloudy- but if it starts clear, then goes cloudy, that's bacteria.


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Re: Research grant syringe - Ps. tampanensis [Re: Doc_T]
    #11679660 - 12/19/09 09:17 AM (14 years, 3 months ago)

If your LCs start cloudy why dont you change the medium to peptone?

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Re: Research grant syringe - Ps. tampanensis [Re: badman]
    #11679666 - 12/19/09 09:18 AM (14 years, 3 months ago)

Because I like dextrose+malt and yeast. I don't really look at LC anyway, you can't tell much by looking.
But they start cloudy and end up clear as the mycelium consumes the nutrients. It's done when it's clear.


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Re: Research grant syringe - Ps. tampanensis [Re: Doc_T]
    #11726791 - 12/27/09 02:59 PM (14 years, 3 months ago)

I don't think this is what it's supposed to look like.



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OfflineShaggy Shaman
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Registered: 11/01/09
Posts: 348
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Re: Research grant syringe - Ps. tampanensis [Re: dancefloordale]
    #11726915 - 12/27/09 03:20 PM (14 years, 3 months ago)

Doesn't look like other pics I have seen....  Both my tamp LC's contamed, the first contams I've had.  My Oak Ridge LC contaminated as well, I noticed yesterday, but that was from a spore print and I did just move from Arizona to Florida as well.  Any suggestions on how I can use 2 contaminated syringes in LC's?  I'm not up to agar yet.


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"Disbelief in magic can force a poor soul into believing in government and business."
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InvisibleM11
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Registered: 06/14/09
Posts: 1,840
Re: Research grant syringe - Ps. tampanensis [Re: Shaggy Shaman]
    #11726985 - 12/27/09 03:35 PM (14 years, 3 months ago)

The myc looks a little thin and whispy.  I would give it a little time.  I don't see any green, black, brown shit growing in there.  You might be fine.  Myc, in my experience, fills in after a few days.

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Invisibledancefloordale
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Registered: 02/13/09
Posts: 2,522
Re: Research grant syringe - Ps. tampanensis [Re: M11]
    #11727064 - 12/27/09 03:50 PM (14 years, 3 months ago)

The pic makes it look more white than grey.

Growth seems to have stalled and looked pretty much the same a week ago when I went home for Christmas.

Never worked with anything besides cubes and this was shot up as a test jar. All the other jars that I shot cube myc/spores into are done without any contams. I'm spawning them tomorrow.


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Everything posted by the user dancefloordale, aside from what is written in this here clause is completely and utterly fictitious, despite any information read (or seen), above (or below) that might lead you to believe otherwise.

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InvisibleM11
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Registered: 06/14/09
Posts: 1,840
Re: Research grant syringe - Ps. tampanensis [Re: dancefloordale]
    #11727077 - 12/27/09 03:54 PM (14 years, 3 months ago)

More gray than white?  Hmmm... If it has stalled that is not a good sign.  If you want, crack open the jar and take a whiff.  It sounds like you won't be spawning it anyway.

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InvisibleCH HELL
Brain Sturgeon
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Registered: 10/02/08
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Re: Research grant syringe - Ps. tampanensis [Re: M11]
    #11728043 - 12/27/09 07:13 PM (14 years, 3 months ago)

I would say that is trich,  even though stone producers have myc similar to that there is a sandy texture in spots.  My guess is that will turn green and yellow,  hang on to it and see what happens but I have a jar identical to that.

Did this syringe just stop moving?  I know who I sent it to,  I was expecting it to branch out and keep going.
Where they at???

CH

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Invisiblewhyblameus
on a mission
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Posts: 11,440
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Re: Research grant syringe - Ps. tampanensis [Re: CH HELL]
    #11728086 - 12/27/09 07:22 PM (14 years, 3 months ago)

it was supposed to come to me but never arrived yet.:shrug:


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Invisibledancefloordale
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Registered: 02/13/09
Posts: 2,522
Re: Research grant syringe - Ps. tampanensis [Re: whyblameus]
    #11728129 - 12/27/09 07:30 PM (14 years, 3 months ago)

It's coming. I was REALLY SLOW sending it out.


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Everything posted by the user dancefloordale, aside from what is written in this here clause is completely and utterly fictitious, despite any information read (or seen), above (or below) that might lead you to believe otherwise.

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Invisibledancefloordale
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Posts: 2,522
Re: Research grant syringe - Ps. tampanensis [Re: dancefloordale]
    #11728138 - 12/27/09 07:32 PM (14 years, 3 months ago)

Hope you have some agar though.


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Everything posted by the user dancefloordale, aside from what is written in this here clause is completely and utterly fictitious, despite any information read (or seen), above (or below) that might lead you to believe otherwise.

Hydra Tek - A detailed guide, for newest to the most skilled cultivators.

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Invisiblewhyblameus
on a mission
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Re: Research grant syringe - Ps. tampanensis [Re: dancefloordale]
    #11728184 - 12/27/09 07:43 PM (14 years, 3 months ago)

Quote:

dancefloordale said:
It's coming. I was REALLY SLOW sending it out.



:facepalm::rotfl:

Quote:

dancefloordale said:
Hope you have some agar though.



i do but:rolleyes:


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Invisibledancefloordale
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Posts: 2,522
Re: Research grant syringe - Ps. tampanensis [Re: whyblameus]
    #11728208 - 12/27/09 07:48 PM (14 years, 3 months ago)

One of the reasons I'm quitting pot. I don't do anything. :facepalm:


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Everything posted by the user dancefloordale, aside from what is written in this here clause is completely and utterly fictitious, despite any information read (or seen), above (or below) that might lead you to believe otherwise.

Hydra Tek - A detailed guide, for newest to the most skilled cultivators.

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