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OfflineMagicMaker
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Re: Early enzyme development in LC [Re: Estario] * 2
    #10988080 - 09/02/09 05:27 PM (14 years, 4 months ago)

good point about the tubing that would seem to protect the filter from getting wet due to swirling, and def easier to replace, more air flow too but i bet just pulling through a hole packs it a lot tighter which would be better to keep out contam, but whatever floats ur boat i can def see wetting the polyfil being an issue


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OfflineEstario
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Gentamicin Sulfate Antibiotic LC [Re: MagicMaker] * 1
    #11143166 - 09/28/09 12:00 PM (14 years, 4 months ago)

I have been adding Gentamicin sulfate to my LC lately. I ad 80 mg of gentamicin to 1250 ml of LC. The mycelium grows as usual and I think those new jars are much more contamination resistant than my older ones. I will try inoculating one of those jars with contaminated LC to see if the bacterial growth is inhibited in those antibiotic LC's.


--------------------
All Of RR's Notes On Mushroom Cultivation Forum - a must read
:alert: Everything I post is completely fictitious, and for your amusement only. All the pictures I post are either photoshopped or ripped from the internet. Whenever i trade for spores it is for examining the spores under microscopes to see their characteristics. There is no reason why I would ever want to nor will I grow mushrooms containing psilocybin. :alert:


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Offlinevishal779
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Re: Gentamicin Sulfate Antibiotic LC [Re: Estario] * 1
    #11509248 - 11/23/09 07:17 AM (14 years, 2 months ago)

hi..just wanted to know if mineral water is the best for lc or distilled water and tap water are equally nice as they are finally going to be sterilised anyway..


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Offlinedman080
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Re: Gentamicin Sulfate Antibiotic LC [Re: vishal779] * 1
    #11509265 - 11/23/09 07:26 AM (14 years, 2 months ago)

SOrry Visual dont know exactly about the mineral water. I did a Karo tek about 4 days ago. And I used Distilled water from a bottle. What I was going to say is mine does not look like that after 4 days. Its my second attempt with LC . No signs of anything but clear right now...


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InvisibleDoc_T
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Re: Gentamicin Sulfate Antibiotic LC [Re: dman080] * 1
    #11509273 - 11/23/09 07:35 AM (14 years, 2 months ago)

Wait 7-14 days to see visible growth from spores.


--------------------
You make it all possible. Doesn't it feel good?


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Invisiblemyceleus_rex
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Re: Early enzyme development in LC [Re: Estario] * 1
    #11509278 - 11/23/09 07:39 AM (14 years, 2 months ago)

Quote:

Estario said:
Here is a little trick I was told by a more experienced grower. The mycelium has many enzymes coded in its DNA and only the ones necessary for the current type of nutrition are being decoded and used. The DNA sequence of the other enzymes is blocked. This means that when you inoculate a rye jar with a honey LC it takes some time for the mycelium to unblock those parts of the DNA and start synthesizing the necessary enzymes. The trick is to add a bit of the substrate you are going to inoculate to the LC so that the mycelium can unblock the DNA sequences and produce the enzymes, which degrade the substrate in adventive.
Take some of the grain you are using and grind it to a fine powder in a coffee grinder. Than you can add just a pinch of this powder to your LC projects. Grindings ensures small size of the grain which is advisable due to the shorter sterilization times associated with LCs. I will test this new tek soon and will post results for sure.




As long as this thread is bumped:

This idea sounds intriguing. The only thing is, I'd think that the little bit of ground-up grain would be inadequately sterilized and could introduce contamination.


--------------------
Cervantes' sclerotia thread    Citric's self-healing lid tek  Agar's Grain LC  Breaking Up Spawn Jars

World's Ugliest Cat?

If ignorance is bliss, why aren't there more happy people?


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Offlinedman080
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Re: Gentamicin Sulfate Antibiotic LC [Re: Doc_T] * 1
    #11509280 - 11/23/09 07:41 AM (14 years, 2 months ago)

Quote:

Doc_T said:
Wait 7-14 days to see visible growth from spores.



Oh ok cause this guy is like 4 days. But he prob use another LC . Thats what makes me love this hobby. Cause you can keep regenerating your stock. . So fucking cool...


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InvisibleDoc_T
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Re: Gentamicin Sulfate Antibiotic LC [Re: dman080] * 1
    #11509291 - 11/23/09 07:49 AM (14 years, 2 months ago)

Quote:

dman080 said:
Oh ok cause this guy is like 4 days. But he prob use another LC .




No. It doesn't matter what you are growing the spores on. They germinate when they are ready. Fresh spores may take off right away.
But wait 7-14 days to see growth from random spores.

And stop staring at your LC. Every time you touch it or breathe on it, you are risking contamination.


--------------------
You make it all possible. Doesn't it feel good?


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Offlinedman080
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Re: Gentamicin Sulfate Antibiotic LC [Re: Doc_T] * 1
    #11509296 - 11/23/09 07:52 AM (14 years, 2 months ago)

Quote:

Doc_T said:
Quote:

dman080 said:
Oh ok cause this guy is like 4 days. But he prob use another LC .




No. It doesn't matter what you are growing the spores on. They germinate when they are ready. Fresh spores may take off right away.
But wait 7-14 days to see growth from random spores.

And stop staring at your LC. Every time you touch it or breathe on it, you are risking contamination.



Nah i dont fuck with it .. i check it evry 2nd day to see if it started yet. Im pretty chill when it comes to waiting for these things. But when he said four days that rAIsed a Red flag. Thanks BTW..


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Invisiblecacharstar
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Re: Early enzyme development in LC [Re: Estario] * 1
    #11603541 - 12/07/09 01:46 PM (14 years, 1 month ago)

Quote:

Estario said:
Here is a little trick I was told by a more experienced grower. The mycelium has many enzymes coded in its DNA and only the ones necessary for the current type of nutrition are being decoded and used. The DNA sequence of the other enzymes is blocked. This means that when you inoculate a rye jar with a honey LC it takes some time for the mycelium to unblock those parts of the DNA and start synthesizing the necessary enzymes. The trick is to add a bit of the substrate you are going to inoculate to the LC so that the mycelium can unblock the DNA sequences and produce the enzymes, which degrade the substrate in adventive.
Take some of the grain you are using and grind it to a fine powder in a coffee grinder. Than you can add just a pinch of this powder to your LC projects. Grindings ensures small size of the grain which is advisable due to the shorter sterilization times associated with LCs. I will test this new tek soon and will post results for sure.




Would this improve the acceptance time of lc to wood lovers substrate as well? 
Also if a culture is taken from fruiting bodies or transfer from agar to agar from the wild, then the DNA should be unlocked right? This applies to germinated spores I assume?:shrug:


--------------------
:irishtoast:


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OfflineEstario
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Re: Early enzyme development in LC [Re: cacharstar] * 1
    #11729409 - 12/28/09 12:37 AM (14 years, 1 month ago)

Quote:

cacharstar said:
Quote:

Estario said:
Here is a little trick I was told by a more experienced grower. The mycelium has many enzymes coded in its DNA and only the ones necessary for the current type of nutrition are being decoded and used. The DNA sequence of the other enzymes is blocked. This means that when you inoculate a rye jar with a honey LC it takes some time for the mycelium to unblock those parts of the DNA and start synthesizing the necessary enzymes. The trick is to add a bit of the substrate you are going to inoculate to the LC so that the mycelium can unblock the DNA sequences and produce the enzymes, which degrade the substrate in adventive.
Take some of the grain you are using and grind it to a fine powder in a coffee grinder. Than you can add just a pinch of this powder to your LC projects. Grindings ensures small size of the grain which is advisable due to the shorter sterilization times associated with LCs. I will test this new tek soon and will post results for sure.




Would this improve the acceptance time of lc to wood lovers substrate as well? 
Also if a culture is taken from fruiting bodies or transfer from agar to agar from the wild, then the DNA should be unlocked right? This applies to germinated spores I assume?:shrug:



Paul Stamets explains this topic pretty extensively in his book Growing Gourmet and Medicinal Mushrooms. He recommends using the soak water from grains to make LC. I have stored some but right now I don't have the need for any more LC so I will use it later. If any one is interested in this topic read page 107.
Quote:

To ameliorate degenerative effects, the
addition of extracted end-substrates (sawdust,
straw, etc.) favors the normal development of
mycehium. The introduction of the end-sub-
strate acquaints the mushroom mycelium with
its destined fruiting habitat, challenging the
mycelium and selectively activating its enzy-
matic systems. This familiarity with the
end-substrate greatly improves performance
later on. Parent cells retain a"genetic memory"
passed downstream through the mycelial net-
works.
Paul Stamets - Growing Gourmet and Medicinal Mushrooms




--------------------
All Of RR's Notes On Mushroom Cultivation Forum - a must read
:alert: Everything I post is completely fictitious, and for your amusement only. All the pictures I post are either photoshopped or ripped from the internet. Whenever i trade for spores it is for examining the spores under microscopes to see their characteristics. There is no reason why I would ever want to nor will I grow mushrooms containing psilocybin. :alert:


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Offlinesantaclausmush
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Registered: 02/25/08
Posts: 263
Last seen: 10 years, 11 months
Re: Honey LC Tek - pictorial [Re: Estario] * 1
    #12217723 - 03/17/10 10:01 AM (13 years, 10 months ago)

Quote:

Estario said:
And here are the results 4 days later.



in this tek did ur mycelium stay and grow at the bottom of the jar? i have six bottles already growing myc. in all of em. but they are all contammed. but they still grow bigger clouds everyday.


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InvisibleRazzl3Frazzl3
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Registered: 07/31/09
Posts: 4,630
Re: Honey LC Tek - pictorial [Re: santaclausmush] * 1
    #12217739 - 03/17/10 10:06 AM (13 years, 10 months ago)

Thanks, how do i sticky this???


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Offlinekurshiukas
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Registered: 02/02/10
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Re: Honey LC Tek - pictorial [Re: santaclausmush] * 1
    #12217963 - 03/17/10 11:01 AM (13 years, 10 months ago)

Quote:

santaclausmush said:
Quote:

Estario said:
And here are the results 4 days later.



in this tek did ur mycelium stay and grow at the bottom of the jar? i have six bottles already growing myc. in all of em. but they are all contammed. but they still grow bigger clouds everyday.




if by contam you mean mold, then the clouds you see are probably mold clouds. Mold myc is the white as shroom myc as long as it does not sporrate.


--------------------
AMU


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Offlinesantaclausmush
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Re: Honey LC Tek - pictorial [Re: kurshiukas] * 1
    #12218053 - 03/17/10 11:14 AM (13 years, 10 months ago)

this is my clouds. there white but im colorblind so i put em in front of my high pressure sodium grow light to see and there white. but i got contam on all of it. this is all good. some i have black dots in and they all are contamed but huge myc clouds it loooks like.


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OfflineMountrakker
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Re: Honey LC Tek - pictorial [Re: Estario] * 1
    #12230860 - 03/19/10 12:35 PM (13 years, 10 months ago)

hey thanks for this great tek, the lids look really prof now

i have done 2 Honey Lc jars by the book/tek but there was too much sediment according to my judgement, is there any chance the flowing around sediment would use as an anchorage for the mycelium? they haven't formed a dense blob as of yet, and they've been in there for more than a week


i like the right jar more, it looks better, and it looks as if it has too types of sediment, the brown (caramelized) and tiny bits and clumps that have formed white hairs on them.
The left one (short one) had too much sediment to begin with (more bio-honey) and i guess it will be discarded

what do you guys think?

now i've got bird seed and popcorn waiting to be pressure cooked and sterilized, as to test the formations in the honey Lc, do you guys think ill have any chance of success? im really new to Lc and in my country(europe) we haven't got Karo products although today at the supermarket i saw some artificial sweetener sugar with a malt-dextrose mix..frustrating..:confused:

peace out


--------------------
Talent hits a target no one else can hit; Genius hits a target no one else can see. Arthur Schopenhauer


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OfflineMountrakker
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Re: Early enzyme development in LC [Re: Estario] * 1
    #12235563 - 03/20/10 06:03 AM (13 years, 10 months ago)

Okay, so the Honey Lc has too much sediment and i'm overconfident with what i do, i guess those two jars in my previous thread have nothing but sediment, and the spores can't get a grip

question : Sedimentation retarded mycelium from forming. The suspended spores should be still vital right?

in this pic i want to make Lc to Lc tranfer, havent done that before


the left one is the one with sediment + spores
the right one is 4% honey properly done

has anybody had success with that? remember i want to do Lc to Lc but with no clear mycleium formation from the first, just the suspended spores (or clumps)

thanks


--------------------
Talent hits a target no one else can hit; Genius hits a target no one else can see. Arthur Schopenhauer


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Offlinesantaclausmush
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Re: Early enzyme development in LC [Re: Mountrakker] * 1
    #12235584 - 03/20/10 06:16 AM (13 years, 10 months ago)

Quote:

Mountrakker said:
Okay, so the Honey Lc has too much sediment and i'm overconfident with what i do, i guess those two jars in my previous thread have nothing but sediment, and the spores can't get a grip

question : Sedimentation retarded mycelium from forming. The suspended spores should be still vital right?

in this pic i want to make Lc to Lc tranfer, havent done that before


the left one is the one with sediment + spores
the right one is 4% honey properly done

has anybody had success with that? remember i want to do Lc to Lc but with no clear mycleium formation from the first, just the suspended spores (or clumps)

thanks


  both jars if u inoculate them will grow a fluffy sediment in it... did u use distilled water... i like using distilled water when i make LC cause theres nothing in the water and it always looks clearer. so u can see the myc better.. but test the LC on one jar first before u go making all these jars then inoculate them and they all grew contaminated.this LC JAR is contaminated...  those other pics are the white fluffy stuff in em but when i inoculated my stuff it was all contam...you get fluffy white stuff if its contammed or not... so b carefull so u dont waste all that time..
THE CLEARER HONEY U CAN FIND THE BETTER.USING DISTILLED WATER IS BETTER.
ive tried 3 kinds of honey and all worked...
just use a test jar to make sure its good...

ne thing elsee i can help u with..he liquid in this jar i used to make LC with cause the liquid and the myc are 100% colonized so i sucked some liquid out of the jar and used it to make LC.  then i took the LC and inoculated one jar with brf and it turned white white so no contam in that LC... make sure u dont contam ur LC JAR before, after or during inoculation.. then u wont know its contamed and all ur jars will be contamed.


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OfflineMountrakker
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Re: Early enzyme development in LC [Re: santaclausmush] * 1
    #12235761 - 03/20/10 08:09 AM (13 years, 10 months ago)

hey thanks for the help, the last 2 hours i've been experimenting like crazy,

a) Lc to Lc

b) Lc to Wild Bird Seed, and Popcorn ! (properly done with polyfill fae, and silicone inoculation points)

c) Alive Pf-tek cake mycelium (3cm x 3cm)(that has produced 3 flushes already) into honey Lc! (glovebox- inner part of cake with sharp knife)

d) Re-Inoculated 10 pf-tek jars after 20 days that show no activity,
with honey lc in question (strange thing is that in the syringe that sucked up the honey lc although didnt contain any myc blobs, i couldn't see any typical black spores floating around, though i shot more that 5-6 cc of dense spore medium 10 days ago)


yes this time i used distilled water, you are right, there is tremendous change in my lc appearance and i hope this time it'll work

thanks for the help and guidance


--------------------
Talent hits a target no one else can hit; Genius hits a target no one else can see. Arthur Schopenhauer


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Offlinesantaclausmush
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Re: Early enzyme development in LC [Re: Mountrakker] * 1
    #12236297 - 03/20/10 10:48 AM (13 years, 10 months ago)

ur LC IS CONTAMINATED.  20days no show nothing CONTAMINATED.  LC THE QUESTION U SAY NO.... contaminated.... the lc if contamed will still show blob of fluffy myc.... so u have to test on one or two jars to make sure. LC is a joke u cant tell if its good or bad. have to test i had eight LC jars. all of em were contaminated.    best wishes and good luck..


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