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InvisibleRonPaulVerm
Piss Christ
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Registered: 07/21/08
Posts: 1,072
Polypropylene Petri's-post contam, pre PC
    #11493293 - 11/20/09 02:32 PM (14 years, 2 months ago)

I have polypropylene petris..so they can be boiled and Pressure cooked.
I poured a bunch of plates and some were done sloppy and as expected some in the btch got contam without even tranferring shit onto them.
I just scraped out the agar and put the petri's into boiling water. They all had tiny contam/agar residue on them.
Now, I want to PC them to re-use...however, should I make sure all residue is off each plate before PCing?
Since i boiled them for 5 -10 minutes will it be alright for me to handle the plates with residue?
ANy thoughts?

p.s. PLEASE do NOT tell me to "just use disposable ones." That's a politicians answer


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Invisiblecyb3rtr0n
searching for truth
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Folding@home Statistics
Registered: 05/06/09
Posts: 1,821
Re: Polypropylene Petri's-post contam, pre PC [Re: RonPaulVerm]
    #11493351 - 11/20/09 02:37 PM (14 years, 2 months ago)

I would think its best to remove all the "residue".

Yes, PCing should kill any contaminates, but if you still have agar on the plate, what is preventing another contaminate spore from reaching the nutritional medium after you PC it?


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OfflineSinoc
Jugglin' fool


Registered: 10/28/09
Posts: 651
Last seen: 3 years, 7 months
Re: Polypropylene Petri's-post contam, pre PC [Re: cyb3rtr0n]
    #11493356 - 11/20/09 02:38 PM (14 years, 2 months ago)

Boy, the thread title sure is a mouthfull lol.


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Invisiblenoobieshroomie
Back again
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Registered: 07/18/08
Posts: 12,769
Loc: Not Too Sure Flag
Re: Polypropylene Petri's-post contam, pre PC [Re: RonPaulVerm]
    #11493359 - 11/20/09 02:39 PM (14 years, 2 months ago)

you want to clean your plates off well
you could wash them with warm soapy water like
you do pots,pans,cups ect...
then dry them off wrap in foil and PC

      -noobie-


--------------------
AMU

Best Thread Ever
CapZilla said:
not sure what GE and FAE are but i should probably get some.

Citric said:
Your signature is wrong on colonization temps!

GOOD JUDGMENT COMES FROM EXPERIENCE
EXPERIENCE COMES FROM BAD JUDGMENT

ROOM TEMP 70-75 IS BEST FOR COLONIZATION
Thank you mycochef


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