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InvisibleRonPaulVerm
Piss Christ
Male

Registered: 07/21/08
Posts: 1,072
Store bought agaricus button on agar. Brown mycelium
    #11431492 - 11/11/09 06:37 AM (14 years, 5 months ago)

I cloned some inner tissue of an Agaricus mushroom to agar.
The following day the tissue turned black...hmmm
Then I saw signs of growth around it soon after.
It looks textured like mycelium except it's a light brown color. Definite brown.
ANyone else experience something like this?


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Spawn I Have: Blewitt, Reishi, Maitake, Chicken of the Woods, Parasol, Shaggy Mane, King, Blue and Gold Oyster, Shiitake, King Stropharia, Lions Mane, Almond Portabello, Elm Oyster, Phoenix Oyster, Nameko, Enoki, White Beech,

Spawn I Want: Corcyceps Sp., Cauliflower Mushrooms, Agaricus species (Portobello), Pink Oyster, Piopinno, ...anything else you might have


Primitive Skills                                     

Any information I've posted is only related to fictional purposes. I do not advocate growing any illegal mushrooms 
...I DO advocate learning about
Law Enforcement Against Prohibition

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InvisibleJef
Out-of-work Sex Slave
Male User Gallery


Registered: 12/02/08
Posts: 764
Loc: near Duncan, BC
Re: Store bought agaricus button on agar. Brown mycelium [Re: RonPaulVerm]
    #11433176 - 11/11/09 01:20 PM (14 years, 5 months ago)

I think you've got a contam.


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I am my own lab rat.


Tell me and I will forget. Show me and I will remember.  Involve me and I will learn.

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Offlinegribi
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Registered: 08/20/08
Posts: 89
Last seen: 12 years, 5 months
Re: Store bought agaricus button on agar. Brown mycelium [Re: RonPaulVerm]
    #11433753 - 11/11/09 02:30 PM (14 years, 5 months ago)

Quote:

RonPaulVerm said:
I cloned some inner tissue of an Agaricus mushroom to agar.
The following day the tissue turned black...hmmm
Then I saw signs of growth around it soon after.
It looks textured like mycelium except it's a light brown color. Definite brown.
ANyone else experience something like this?




I dropped some inner tissue of a. bisporus on agar about a week ago. Both pieces turned black so I figured they died. However, a few days later, nice white mycelium started stretching out from around the dead looking pieces. It's definitely white, not brown though.

Pic of growth:


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I am looking for the following edible cultures:
  • Chicken of the woods (Laetiporus sulphureus)
  • Wood blewit (Lepista nuda)
  • Cauliflower (Sparassis crispa)

My trade list of available cultures is in my Journal.

Edited by gribi (11/12/09 11:35 AM)

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OfflineM8M
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Registered: 05/28/09
Posts: 229
Last seen: 9 days, 21 hours
Re: Store bought agaricus button on agar. Brown mycelium [Re: RonPaulVerm]
    #11442241 - 11/12/09 04:42 PM (14 years, 5 months ago)

Check out the agar culture plates on this site and you will see quite a few Agaricus plates have brown mycelium, particularly if on PDA.  You can search for the various species and see plates on various mediums.

http://kacc.rda.go.kr/eng/menu2/img_list_resultnew_result.asp?l_code=2759-10-1-4-2-3-2-2-1-5

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InvisibleJef
Out-of-work Sex Slave
Male User Gallery


Registered: 12/02/08
Posts: 764
Loc: near Duncan, BC
Re: Store bought agaricus button on agar. Brown mycelium [Re: M8M]
    #11445420 - 11/12/09 11:43 PM (14 years, 5 months ago)

It looks like Gribi's mycelium has some brown to it too.  I wonder what that is, some kind of metabolite ?

Thanks to M8M for sharing the terrific resource.  It could come in very handy in the future.

Jef


--------------------
I am my own lab rat.


Tell me and I will forget. Show me and I will remember.  Involve me and I will learn.

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InvisibleRonPaulVerm
Piss Christ
Male

Registered: 07/21/08
Posts: 1,072
Re: Store bought agaricus button on agar. Brown mycelium [Re: Jef]
    #11446302 - 11/13/09 06:42 AM (14 years, 5 months ago)

yeah, thanks for the source!
I might get some pics up.
I'm just too excited about my Laetiporus Sulphereus isolate...
I hope to trade it for some shiitake myc on agar. (or lions mane or enoki)
I could by plug sawn but it's just so cool and fun growing it out on my own


--------------------
Spawn I Have: Blewitt, Reishi, Maitake, Chicken of the Woods, Parasol, Shaggy Mane, King, Blue and Gold Oyster, Shiitake, King Stropharia, Lions Mane, Almond Portabello, Elm Oyster, Phoenix Oyster, Nameko, Enoki, White Beech,

Spawn I Want: Corcyceps Sp., Cauliflower Mushrooms, Agaricus species (Portobello), Pink Oyster, Piopinno, ...anything else you might have


Primitive Skills                                     

Any information I've posted is only related to fictional purposes. I do not advocate growing any illegal mushrooms 
...I DO advocate learning about
Law Enforcement Against Prohibition

Extras: Filter Print Post Top
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