Home | Community | Message Board


This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Original Sensible Seeds Bulk Cannabis Seeds   Myyco.com Golden Teacher Liquid Culture For Sale   PhytoExtractum Maeng Da Thai Kratom Leaf Powder   Left Coast Kratom Buy Kratom Extract   North Spore Bulk Substrate   Mushroom-Hut Liquid Cultures   Kraken Kratom Red Vein Kratom   MagicBag.co All-In-One Bags That Don't Suck

Jump to first unread post Pages: 1
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
OfflineMigraine
Expanding Life
 User Gallery

Registered: 09/21/09
Posts: 158
Loc: Alabama
Last seen: 4 months, 19 days
Spores to LC
    #11239292 - 10/13/09 12:40 PM (14 years, 5 months ago)

I received a spore print and I don't know how clean it is.

I have made 5 agar dishes from the spores with the intention for going to grain if no contams.

I have just made an LC solution which I would like to also use with the spores.

My problem is - how best to move spores into the LC without bringing any contams.

#1.  Hold print over LC jar and scrape.

#2.  Scrape spores into a small shot glass containing low percentage hydrogen peroxide - then transfer that solution into the LC.

#3.  Sterile water puddle on print and move solution to LC.

Any thoughts would be helpful - contams love LC ... I want to keep them out.

Thanks

Extras: Filter Print Post Top
Invisiblemyco_mikey_mo
Mycologist For Hire
Male User Gallery


Registered: 09/11/09
Posts: 1,460
Loc: The PNW
Re: Spores to LC [Re: Migraine]
    #11239346 - 10/13/09 12:49 PM (14 years, 5 months ago)

H2O2 kills spores.


--------------------
-Give a man some mushies, He'll trip for a day...Teach a man to cultivate, He'll trip for a lifetime-
:thumbup:Self healing Tyvek and Silcone lids for Trade... PM :pm: me:thumbup:
:syringe:Looking for anybody with extra spawn bags for trade... PM:pm: me:syringe:

Extras: Filter Print Post Top
Invisiblemyco_mikey_mo
Mycologist For Hire
Male User Gallery


Registered: 09/11/09
Posts: 1,460
Loc: The PNW
Re: Spores to LC [Re: myco_mikey_mo]
    #11239435 - 10/13/09 01:07 PM (14 years, 5 months ago)

and i would just reccomend streaking the spores with an inoculation loop or something like that. honestly though i have always just made a syringe and squirted some solution in my LC. works great for me. also a glovebox or flow hood would really help


--------------------
-Give a man some mushies, He'll trip for a day...Teach a man to cultivate, He'll trip for a lifetime-
:thumbup:Self healing Tyvek and Silcone lids for Trade... PM :pm: me:thumbup:
:syringe:Looking for anybody with extra spawn bags for trade... PM:pm: me:syringe:

Extras: Filter Print Post Top
Offlinesolumvita
Q.B.E.
Male User Gallery

Registered: 02/12/08
Posts: 2,061
Loc: South Africa
Last seen: 9 months, 16 days
Trusted Cultivator
Re: Spores to LC [Re: myco_mikey_mo]
    #11239467 - 10/13/09 01:14 PM (14 years, 5 months ago)

wait until the spores on the plates have germinated and are visibly clear from contams and then transfer a piece from a clean plate into the LC broth.


--------------------
One of these days all the answers will be revealed until then we learn from each other!

www.mushrush.co.za

Extras: Filter Print Post Top
OfflineMigraine
Expanding Life
 User Gallery

Registered: 09/21/09
Posts: 158
Loc: Alabama
Last seen: 4 months, 19 days
Re: Spores to LC [Re: solumvita]
    #11240080 - 10/13/09 02:59 PM (14 years, 5 months ago)

Thanks for the replies.

I do have and use a flow hood.

I wanted to run a fast path so I scraped and dropped spores directly into the LC.

Contam - time will tell.

I will do one more from my agar when they grow out.

Thanks all.

Extras: Filter Print Post Top
Invisible13shroomsM
Lightning Shaman
I'm a teapot User Gallery


Registered: 01/01/09
Posts: 26,719
Loc: IN ETHERS TORSION FIELD
Trusted Cultivator
Re: Spores to LC [Re: Migraine]
    #11246400 - 10/14/09 02:26 PM (14 years, 5 months ago)



--------------------
:takingnotes:A M U:helpdesk:
Click here ^ for the AMU forum
VVV AMUs Free Active/Edible/Exotic Spore Print or Syringe or Edible Culture Trade Thread VVV
"Man is the sex organ of the machine world" ~  Marshall McLuhan

Extras: Filter Print Post Top
OfflinePuma
surfing when I can
Male


Registered: 10/08/09
Posts: 319
Loc: BC
Last seen: 4 months, 3 days
Re: Spores to LC [Re: 13shrooms]
    #11444627 - 11/12/09 09:33 PM (14 years, 4 months ago)

Well, how did it turn out? You've kept us in suspense long enough...!

Out with it!

Photos? What's the status now of that grow?


--------------------

Extras: Filter Print Post Top
Invisible13shroomsM
Lightning Shaman
I'm a teapot User Gallery


Registered: 01/01/09
Posts: 26,719
Loc: IN ETHERS TORSION FIELD
Trusted Cultivator
Re: Spores to LC [Re: Puma]
    #11444686 - 11/12/09 09:40 PM (14 years, 4 months ago)

:lmafo:


--------------------
:takingnotes:A M U:helpdesk:
Click here ^ for the AMU forum
VVV AMUs Free Active/Edible/Exotic Spore Print or Syringe or Edible Culture Trade Thread VVV
"Man is the sex organ of the machine world" ~  Marshall McLuhan

Extras: Filter Print Post Top
OfflineMigraine
Expanding Life
 User Gallery

Registered: 09/21/09
Posts: 158
Loc: Alabama
Last seen: 4 months, 19 days
Re: Spores to LC [Re: 13shrooms]
    #11453473 - 11/14/09 12:13 PM (14 years, 4 months ago)

Sorry for delay - The spore print was from a Golden Teacher.

The five quart jars of rye - from agar dishes are only at 70% colonized - so I have not taken them to substrate yet.

I dropped spores into a 4% light malt LC solution.  After 20 days small islands of mycelium started forming on the surface.  I placed the LC on a magnetic stirrer for two minutes every day.  I have placed the LC in the frig - may not use it. In Aug I made a similar LC of King Oyster and Lions Mane.  I had to leave home for a month - so no stiiring.  Before I left I had the same formation taking place - I though it was contam - that is why I just left it.  To my suprise, when I returned, I found a three inch thick mycelium slab sitting on the surface of the LC.


My goal now will be to fruit from the rye jars and clone to agar from a nice looking mushroom.  Once I know I have a contam free dish - I will take it to slants (for my library) and then a few small rye jars.  Once those rye jars have colonized, I will extract mycelium from one and use IT as my LC master as Agar outlined in one of his posts.

I have read almost every one of Agar's threads - I don't know how I missed that one - Thanks 13shrooms for pointing it out.

Pics when I have something interesting.

Extras: Filter Print Post Top
InvisibleBlimeyGrimey
Collector of Spores
Male


Folding@home Statistics
Registered: 08/24/05
Posts: 3,796
Loc: Puget Sound
Re: Spores to LC [Re: 13shrooms]
    #11459904 - 11/15/09 01:13 PM (14 years, 4 months ago)



--------------------
Message me for free microscopy services on Psilocybe, Panaeolus, and Gymnopilus species.

Looking for wild Panaeolus cinctulus and Panaeolus olivaceus prints.

Extras: Filter Print Post Top
OfflinePuma
surfing when I can
Male


Registered: 10/08/09
Posts: 319
Loc: BC
Last seen: 4 months, 3 days
Re: Spores to LC [Re: BlimeyGrimey]
    #11464355 - 11/15/09 11:27 PM (14 years, 4 months ago)

Interesting update. Thanks for that I guess maybe spore-to-LC isn't really the fast path after all. I thought it would be too, but after ten days I'm seeing very little formation, other than a few small squiggles, and a sort of white film along the inside edge of the jar, at the surface of the liquid. I wonder if it's definitely growth, possibly contam, or just residue of malt extract. Hopefully it'll soon be more obvious.

That's quite something, the puck of mycelium from those jars you left!

It sounds like agar media and grain might be preferable to dropping spores in liquid solution. Although I spent all my Orissa spores on the LC attempt (so I'm hoping it pans out) I'll make LC starting with another strain, using RR's preferred technique of adding sterile water to a colonized grain jar, shaking it up, and charging syringes with the resulting myc-rich water. It seems like that's the best way to speed up the time (as grains colonize faster than water) as well as minimizing the odds of contam, as you can inspect visually... as others here have noted.

Best of luck with your cultures. I would be tempted to drop some of that LC in your fridge into some agar to see if its clean.


--------------------

Extras: Filter Print Post Top
InvisibleBlimeyGrimey
Collector of Spores
Male


Folding@home Statistics
Registered: 08/24/05
Posts: 3,796
Loc: Puget Sound
Re: Spores to LC [Re: Puma]
    #11465194 - 11/16/09 04:30 AM (14 years, 4 months ago)

Quote:

Puma said:
I thought it would be too, but after ten days I'm seeing very little formation, other than a few small squiggles, and a sort of white film along the inside edge of the jar, at the surface of the liquid.




Sounds like bacterial contamination.


--------------------
Message me for free microscopy services on Psilocybe, Panaeolus, and Gymnopilus species.

Looking for wild Panaeolus cinctulus and Panaeolus olivaceus prints.

Extras: Filter Print Post Top
OfflinePuma
surfing when I can
Male


Registered: 10/08/09
Posts: 319
Loc: BC
Last seen: 4 months, 3 days
Re: Spores to LC [Re: BlimeyGrimey]
    #11465235 - 11/16/09 04:56 AM (14 years, 4 months ago)

Uh oh.....

Here's what it looks like. I'm clinging to the hope that it might be mycelium growing up there because that's where there's access to oxygen (although I've been shaking too). The white gummy stuff flakes off sometimes when shaken.







--------------------

Extras: Filter Print Post Top
Jump to top Pages: 1

Shop: Original Sensible Seeds Bulk Cannabis Seeds   Myyco.com Golden Teacher Liquid Culture For Sale   PhytoExtractum Maeng Da Thai Kratom Leaf Powder   Left Coast Kratom Buy Kratom Extract   North Spore Bulk Substrate   Mushroom-Hut Liquid Cultures   Kraken Kratom Red Vein Kratom   MagicBag.co All-In-One Bags That Don't Suck


Similar ThreadsPosterViewsRepliesLast post
* Agar&LC
( 1 2 all )
Aiko Aiko 3,460 23 10/09/05 09:39 AM
by Blue Helix
* Constant-stir LC showing expotential growth in first 3.5 days. (pics)
( 1 2 3 4 5 all )
Blue Helix 27,902 96 07/31/05 09:07 AM
by scatmanrav
* Agar to LC xburn 1,748 6 05/25/05 01:44 AM
by Blue Helix
* Would spores carry over the isolation? HidingInPlainSight 490 1 05/11/05 11:03 PM
by RogerRabbit
* Spore mass slurry ambros 18,210 15 10/31/15 01:07 AM
by micro
* PDYA LC question Lapius 1,849 5 07/06/05 11:23 AM
by Roadkill
* Does H2O2 KILL spores or just inhibit germination?
( 1 2 all )
micro 7,077 37 11/26/03 09:24 AM
by debianlinux
* The Return of the Red Spore Cubensis
( 1 2 3 4 5 6 all )
RogerRabbitM 28,637 104 05/10/10 03:05 PM
by total

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: RogerRabbit, Pastywhyte, bodhisatta
3,446 topic views. 0 members, 6 guests and 2 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.024 seconds spending 0.006 seconds on 14 queries.