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Amazon Shop: ½ Pint Jars, Agar, Wild Bird Seed

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OfflineHarlz
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just knocked up an LC
    #11418865 - 11/09/09 03:07 PM (8 years, 1 month ago)

Mixture

1 packet, about 3.4 grams of turbinado raw sugar
about 230 ml of filtered brita, tap water

anyone tried this LC?  Agars tek shows some nice clouds, but hes a pro.
By the way the syringe I used was homemade so it was thick with spores, and I can see a bunch floating in the solution with only about 2 or 3 cc used :thumbup::vaped:


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OfflineBrandNewbie
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Re: just knocked up an LC [Re: Harlz]
    #11418892 - 11/09/09 03:11 PM (8 years, 1 month ago)

Have not tried it.  I disagree with LC's.  I'd rather use myceliated water. :2cents:

Good luck to you though! :cheers:


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Re: just knocked up an LC [Re: Harlz]
    #11418896 - 11/09/09 03:11 PM (8 years, 1 month ago)

Wow- that's about 10x the spores you need. Just so you know for next time. :smile:


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Re: just knocked up an LC [Re: Harlz]
    #11418897 - 11/09/09 03:11 PM (8 years, 1 month ago)

Should use 4 grams to 100mls of water also dark syringes are not necessarily good only 1 cc should have been used


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Re: just knocked up an LC [Re: Shea25]
    #11418900 - 11/09/09 03:12 PM (8 years, 1 month ago)

4:100 max. Less is ok.


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OfflineHarlz
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Re: just knocked up an LC [Re: Doc_T]
    #11419751 - 11/09/09 05:22 PM (8 years, 1 month ago)

I know the print i used was ridiculously thick and i took too much , but oh well :shrug:

I have a jar of myc water in the fridge I just wanted to use my new glovebox and I was bored:thumbup:


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Invisibledaytripper05
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Re: just knocked up an LC [Re: BrandNewbie]
    #11419788 - 11/09/09 05:28 PM (8 years, 1 month ago)

Quote:

BrandNewbie said:
Have not tried it.  I disagree with LC's.  I'd rather use myceliated water. :2cents:




:thumbup:

I have tried it, and I prefer Grain LC's to sugar water LC by far.


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Offlinesmurpht
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Re: just knocked up an LC [Re: BrandNewbie]
    #11419794 - 11/09/09 05:28 PM (8 years, 1 month ago)

Quote:

BrandNewbie said:
Have not tried it.  I disagree with LC's.  I'd rather use myceliated water. :2cents:

Good luck to you though! :cheers:




sorry but what's myc water? is that grain lc? or something else?

i'm confused, what's the difference between myc water and a LC?


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Re: just knocked up an LC [Re: smurpht]
    #11419803 - 11/09/09 05:30 PM (8 years, 1 month ago)

grain LC or i put water in my agar jars and shake for myc water that way cuz i can do it over and over


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Re: just knocked up an LC [Re: smurpht]
    #11419829 - 11/09/09 05:33 PM (8 years, 1 month ago)

A sugar water LC is a medium to grow mycelium. It's almost impossible to tell if it's clean without a test jar. The mycelium grows slows in water than on grains.

So if you get something that is 100% colonized, take some of the tissue and suspend it in water, you have an instant mycelium syringe that you know to be sterile (with good procedure of course). You don't need a test jar so you really save a lot time. Sugar water LC's aren't efficient in any way.

They work fine, but there are better ways to do the same task, and that is to make lots of mycelium suspended in water.


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Re: just knocked up an LC [Re: feelfunny]
    #11419836 - 11/09/09 05:34 PM (8 years, 1 month ago)

Quote:

feelfunny said:
grain LC or i put water in my agar jars and shake for myc water that way cuz i can do it over and over




Nice, I am going to do this. I have been thinking about doing it but didn't know how it would turn out.


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Offlinesmurpht
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Re: just knocked up an LC [Re: feelfunny]
    #11419849 - 11/09/09 05:35 PM (8 years, 1 month ago)

Quote:

feelfunny said:
grain LC or i put water in my agar jars and shake for myc water that way cuz i can do it over and over




that's awsome, i'm so using that agar trick. cuz agar is easy i find with the grocery store tek and some self healing lids, easier than preparing grains. u guys rock!


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Re: just knocked up an LC [Re: daytripper05]
    #11419851 - 11/09/09 05:35 PM (8 years, 1 month ago)

yea i went back to useing jars for agar since you can PC them and i use the RTV lids so i dont have to open them


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Re: just knocked up an LC [Re: feelfunny]
    #11419901 - 11/09/09 05:43 PM (8 years, 1 month ago)

Yeah, I like the idea of that. The tall pint jars, the smallest ones I have, kinda suck. I want the 1/2's or the jelly jars.

Not to get off topic of the thread, to the OP, I would go to the health food store and get some agar agar (vegetable gelatin) and some ordinary potato flakes. Making up agar dishes (with jars) is just as easy as making LC's and the results are way better. I don't see why sugar water LC's are so popular above agar. Agar seems to have a stigma that's it's more advanced or something which is totally not true IMO. It CAN lead to more advanced things, but the preparation and using it to germinate spores dominates all and is as easy as anything else.


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Re: just knocked up an LC [Re: daytripper05]
    #11419930 - 11/09/09 05:47 PM (8 years, 1 month ago)

Never really thought about it like that- agar in a jar to make myc watter. Hm... :strokebeard:


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Offlinegornyhuy
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Re: just knocked up an LC [Re: daytripper05]
    #11419967 - 11/09/09 05:52 PM (8 years, 1 month ago)

I've only done sugar LCs so far, but they have worked so well its ridiculous.  I know myc water is supposed to be better, but I didn't have any grains knocked up in the first place and I wanted to do them from myc, not from spores, just because I hadn't tried it before.

My half assed recipe:
-pint jar
-about 1/4 pint water
-about 1/4 pint 2nd brewed coffee
-about tablespoon (less is probably better) honey

PC 20 minutes, inject 1 to 2 CCs spore solution (less is probably plenty)
incubate at 70-75 F

Clouds of myc show within a couple days, really big thick clouds within a week, color from coffee begins to fade as myc consumes it after week 1.

Personally, I use a home made magnetic stir and it makes a big difference I think.

Injecting this into a grain jar (even a crappy over cooked over soaked grain jar) gets huge myc grow in a day or two.


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Re: just knocked up an LC [Re: gornyhuy]
    #11420026 - 11/09/09 06:04 PM (8 years, 1 month ago)

I guess you won't know till you try them both. Grain LC's or any method of making mycelium water is easier and takes grossly less time in the long run. When you make sugar water LC's the mycelium has to adjust to growing on a solid medium rather than liquid. Mycelium grown on grains suspended in water don't need to adjust. IME the grain LC's were 2-3 days faster to colonize quarts of WBS.


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Offlinegornyhuy
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Re: just knocked up an LC [Re: daytripper05]
    #11433425 - 11/11/09 03:50 PM (8 years, 1 month ago)

Quote:

daytripper05 said:
I guess you won't know till you try them both. Grain LC's or any method of making mycelium water is easier and takes grossly less time in the long run. When you make sugar water LC's the mycelium has to adjust to growing on a solid medium rather than liquid. Mycelium grown on grains suspended in water don't need to adjust. IME the grain LC's were 2-3 days faster to colonize quarts of WBS.





Grain LC is my next project!  Can't wait.
Unfortunately I just NOW learned the hard way that PEROXIDE WILL KILL YOUR LC MYCELIUM!!

I was worried about my sugar LC getting contam'd and I had a little floater growing something. (Probably it was just a little grain of something with myc growing on it) So I got all sterile and injected about 5 CCs of peroxide on the floater and washed down the sides of the LC jar with it.  BAM killed the whole thing. 

Before, the mycelium would kind of settle into a thick cloud on the bottom until I ran the magnetic stir.  But now, less than an hour after I injected that shit, it is ALL floating on the top.  THEN I read RR say that peroxide is toxic to mycelium, particularly in an LC.  D'OH!!!!

Luckily I'd already gotten quite a bit of successful use out of it.


So - don't put peroxide in your LC.


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Re: just knocked up an LC [Re: gornyhuy]
    #11436360 - 11/11/09 09:54 PM (8 years, 1 month ago)

Quote:

gornyhuy said:
I had a little floater growing something.




Generally, floating myc in LC is bad news.


Quote:

gornyhuy said:
Luckily I'd already gotten quite a bit of successful use out of it.




Every time you tap that jar, you are risking the rest of it. Best practice is to draw it all up at once in separate syringes and discard the rest.
If you keep going back to it, you'll get a contam sooner or later, and it will hit by surprise.


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Offlinecyb3rtr0n
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Re: just knocked up an LC [Re: Harlz]
    #11438157 - 11/12/09 01:58 AM (8 years, 1 month ago)

1.4% nutrient in a LC seems kinda low. :shrug:


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Amazon Shop: ½ Pint Jars, Agar, Wild Bird Seed

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