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Florida
Soul Rebel
Registered: 04/30/09
Posts: 149
Loc: Florida
Last seen: 11 years, 9 months
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Inoculating a Jar with Several Different Sub-strains
#11418585 - 11/09/09 12:33 PM (14 years, 4 months ago) |
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What would happen if you inoculated a jar with 10 different sub-strains with .5 CC of spore solution each? Like B+, Burma, Ecuador, Golden Teacher, Mazatapec, PES Amazonian, Penis Envy, Treasure Coast, Menace, Colombian. I guess one would have to trump all the rest.
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Herbal_Elixer
Strangerest
Registered: 05/05/09
Posts: 1,841
Loc: Reykjavik
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Re: Inoculating a Jar with Several Different Sub-strains [Re: Florida]
#11418605 - 11/09/09 12:35 PM (14 years, 4 months ago) |
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I have no idea.
Quote:
RogerRabbit said: First things first. Mushrooms strains don't 'compete' with each other.
Second, as said above, an isoate is an isolate. It will be one organism.
If you have several strains active in a jar of grains, most will combine into a single organism via the process of anastomosis.(Google it) The others will fruit as separate strains, but won't be 'competing for nutes' as we hear the noobs constantly saying. In fact, I've shot several strains deliberately into a single jar to watch what happens. Most combine, but now and then you get a substrate with obviously different genetics in the fruiting bodies. For example, you might have several large solo mushrooms, while the rest of the substrate is covered with smaller fruits coming from clusters. Usually however, the difference between strains is so minimal that you'll never notice.
Shaking the jar won't cause the non-compatible strains to join forces. The similar mycelium will re-connect with like mycelium. The entire concept of 'strain' in the OMC is vastly overrated. It's nothing like in mj, where the strain determines everything. Remember, fungi are not plants. Perhaps an analogy, since most 'strains' seem to be named for where they were collected is to consider a man from Chicago (Chicago strain), getting married to a woman from New York (New York strain). The genes from the two strains combine, rather than fight. RR
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feelfunny
I am you
Registered: 03/11/09
Posts: 8,747
Loc: South
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Re: Inoculating a Jar with Several Different Sub-strains [Re: Herbal_Elixer]
#11418615 - 11/09/09 12:37 PM (14 years, 4 months ago) |
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the one that will win will just be the strongest of the samples and will probly be different every time.
-------------------- IF A CAT AND DOG CAN GET ALONG WHY CANT EVERYONE ELSE? If the sky is falling, don't look up! Feel Family Founder. me if you are tired of hearing, "Use the search function".
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Shea25
Just some guy
Registered: 01/27/09
Posts: 7,772
Loc: Westcoast Canada
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Re: Inoculating a Jar with Several Different Sub-strains [Re: Florida]
#11418616 - 11/09/09 12:37 PM (14 years, 4 months ago) |
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There would be too much moisture and a better chance of it contaming
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Doc_T
Random Dude
Registered: 03/06/09
Posts: 42,395
Loc: Colorado
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Re: Inoculating a Jar with Several Different Sub-strains [Re: Shea25]
#11418623 - 11/09/09 12:38 PM (14 years, 4 months ago) |
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Way too many spores, you'd get a clusterfuck of a grow out of that.
Less is more. Use the fewest spores possible. Two, ideally.
-------------------- You make it all possible. Doesn't it feel good?
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Florida
Soul Rebel
Registered: 04/30/09
Posts: 149
Loc: Florida
Last seen: 11 years, 9 months
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Re: Inoculating a Jar with Several Different Sub-strains [Re: Doc_T]
#11418664 - 11/09/09 12:43 PM (14 years, 4 months ago) |
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someone with a healthy spore inventory should do this experiment.
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Doc_T
Random Dude
Registered: 03/06/09
Posts: 42,395
Loc: Colorado
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Re: Inoculating a Jar with Several Different Sub-strains [Re: Florida]
#11418679 - 11/09/09 12:45 PM (14 years, 4 months ago) |
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There's no need. It's not an experiment, it's just goofing around with spores. You've got spores, give it a try.
-------------------- You make it all possible. Doesn't it feel good?
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truskool
WTF?? FTW!!
Registered: 06/28/09
Posts: 11,194
Loc: Over the rainbow
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Re: Inoculating a Jar with Several Different Sub-strains [Re: Florida]
#11418693 - 11/09/09 12:46 PM (14 years, 4 months ago) |
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I am willing to do it right now. I got some jars of rye ready and have been deciding what to inoculate with
-------------------- Ask AMU for the best mycology advice out there Roll it While I troll it. I don't mean to boast, but dam if I don't brag.
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Florida
Soul Rebel
Registered: 04/30/09
Posts: 149
Loc: Florida
Last seen: 11 years, 9 months
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Re: Inoculating a Jar with Several Different Sub-strains [Re: Shea25]
#11418745 - 11/09/09 12:53 PM (14 years, 4 months ago) |
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Quote:
Shea25 said: There would be too much moisture and a better chance of it contaming
True, but your missing the point here. What if the spore to substrate ratios were proportional to a single strain inoculation?
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truskool
WTF?? FTW!!
Registered: 06/28/09
Posts: 11,194
Loc: Over the rainbow
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Re: Inoculating a Jar with Several Different Sub-strains [Re: Florida]
#11418762 - 11/09/09 12:54 PM (14 years, 4 months ago) |
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ima put like 2-3 drops of each into a jar
-------------------- Ask AMU for the best mycology advice out there Roll it While I troll it. I don't mean to boast, but dam if I don't brag.
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Florida
Soul Rebel
Registered: 04/30/09
Posts: 149
Loc: Florida
Last seen: 11 years, 9 months
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Re: Inoculating a Jar with Several Different Sub-strains [Re: truskool]
#11418765 - 11/09/09 12:55 PM (14 years, 4 months ago) |
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Awesome. If you are serious! Make a thread I'm sure a lot of people would be interested.
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truskool
WTF?? FTW!!
Registered: 06/28/09
Posts: 11,194
Loc: Over the rainbow
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Re: Inoculating a Jar with Several Different Sub-strains [Re: Florida]
#11418772 - 11/09/09 12:55 PM (14 years, 4 months ago) |
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Yea ill throw it up in a couple hours
-------------------- Ask AMU for the best mycology advice out there Roll it While I troll it. I don't mean to boast, but dam if I don't brag.
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nachohippie
asshole
Registered: 09/19/09
Posts: 2,623
Loc: right around the corner
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Re: Inoculating a Jar with Several Different Sub-strains [Re: feelfunny]
#11418778 - 11/09/09 12:56 PM (14 years, 4 months ago) |
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Quote:
feelfunny said: the one that will win will just be the strongest of the samples and will probly be different every time.
sounds about right to me
-------------------- send guns, money ,lawyers, and drugs its been a long night everything i post is a lie im a pathological liar
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Florida
Soul Rebel
Registered: 04/30/09
Posts: 149
Loc: Florida
Last seen: 11 years, 9 months
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Re: Inoculating a Jar with Several Different Sub-strains [Re: truskool]
#11418786 - 11/09/09 12:57 PM (14 years, 4 months ago) |
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Quote:
truskool said: Yea ill throw it up in a couple hours
I changed my mood.
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truskool
WTF?? FTW!!
Registered: 06/28/09
Posts: 11,194
Loc: Over the rainbow
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Re: Inoculating a Jar with Several Different Sub-strains [Re: Florida]
#11418805 - 11/09/09 12:59 PM (14 years, 4 months ago) |
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Yea i doubt anyone will be able to tell the difference of any of the fruits I am cooking up a bunch of jars and thought that it would be fun to try one jar like this
-------------------- Ask AMU for the best mycology advice out there Roll it While I troll it. I don't mean to boast, but dam if I don't brag.
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apoonanor
Time & Space Traveler
Registered: 04/20/09
Posts: 380
Last seen: 9 years, 10 months
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Re: Inoculating a Jar with Several Different Sub-strains [Re: truskool]
#11420666 - 11/09/09 05:43 PM (14 years, 4 months ago) |
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inoculating jars with multiple syringes will most likely produce generic looking cubes that will most likely act like generic looking cubes. given that they are all multispore inoculations, your results will look like cubes, act like cubes, and behave like...well, cubes.
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truskool
WTF?? FTW!!
Registered: 06/28/09
Posts: 11,194
Loc: Over the rainbow
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Re: Inoculating a Jar with Several Different Sub-strains [Re: apoonanor]
#11420690 - 11/09/09 05:46 PM (14 years, 4 months ago) |
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-------------------- Ask AMU for the best mycology advice out there Roll it While I troll it. I don't mean to boast, but dam if I don't brag.
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