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OfflineFlorida
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Registered: 04/30/09
Posts: 149
Loc: Florida
Last seen: 11 years, 9 months
Inoculating a Jar with Several Different Sub-strains
    #11418585 - 11/09/09 12:33 PM (14 years, 4 months ago)

What would happen if you inoculated a jar with 10 different sub-strains with .5 CC of spore solution each? Like B+, Burma, Ecuador, Golden Teacher, Mazatapec, PES Amazonian, Penis Envy, Treasure Coast, Menace, Colombian. I guess one would have to trump all the rest.
Which One Would Win?
You may choose only one


Votes accepted from (11/09/09 12:33 PM) to (No end specified)
View the results of this poll



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InvisibleHerbal_Elixer
Strangerest
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Registered: 05/05/09
Posts: 1,841
Loc: Reykjavik
Re: Inoculating a Jar with Several Different Sub-strains [Re: Florida]
    #11418605 - 11/09/09 12:35 PM (14 years, 4 months ago)

I have no idea.

Quote:

RogerRabbit said:
First things first.  Mushrooms strains don't 'compete' with each other.

Second, as said above, an isoate is an isolate.  It will be one organism.

If you have several strains active in a jar of grains, most will combine into a single organism via the process of anastomosis.(Google it)  The others will fruit as separate strains, but won't be 'competing for nutes' as we hear the noobs constantly saying.  In fact, I've shot several strains deliberately into a single jar to watch what happens.  Most combine, but now and then you get a substrate with obviously different genetics in the fruiting bodies.  For example, you might have several large solo mushrooms, while the rest of the substrate is covered with smaller fruits coming from clusters.  Usually however, the difference between strains is so minimal that you'll never notice.

Shaking the jar won't cause the non-compatible strains to join forces.  The similar mycelium will re-connect with like mycelium.  The entire concept of 'strain' in the OMC is vastly overrated.  It's nothing like in mj, where the strain determines everything.  Remember, fungi are not plants.  Perhaps an analogy, since most 'strains' seem to be named for where they were collected is to consider a man from Chicago (Chicago strain), getting married to a woman from New York (New York strain).  The genes from the two strains combine, rather than fight.
RR




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Invisiblefeelfunny
I am you
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Registered: 03/11/09
Posts: 8,747
Loc: South
Re: Inoculating a Jar with Several Different Sub-strains [Re: Herbal_Elixer]
    #11418615 - 11/09/09 12:37 PM (14 years, 4 months ago)

the one that will win will just be the strongest of the samples and will probly  be different every time.


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InvisibleShea25
Just some guy
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Registered: 01/27/09
Posts: 7,772
Loc: Westcoast Canada Flag
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Re: Inoculating a Jar with Several Different Sub-strains [Re: Florida]
    #11418616 - 11/09/09 12:37 PM (14 years, 4 months ago)

There would be too much moisture and a better chance of it contaming

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InvisibleDoc_T
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Registered: 03/06/09
Posts: 42,395
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Re: Inoculating a Jar with Several Different Sub-strains [Re: Shea25]
    #11418623 - 11/09/09 12:38 PM (14 years, 4 months ago)

Way too many spores, you'd get a clusterfuck of a grow out of that.

Less is more. Use the fewest spores possible. Two, ideally.


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OfflineFlorida
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Registered: 04/30/09
Posts: 149
Loc: Florida
Last seen: 11 years, 9 months
Re: Inoculating a Jar with Several Different Sub-strains [Re: Doc_T]
    #11418664 - 11/09/09 12:43 PM (14 years, 4 months ago)

someone with a healthy spore inventory should do this experiment.


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InvisibleDoc_T
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Re: Inoculating a Jar with Several Different Sub-strains [Re: Florida]
    #11418679 - 11/09/09 12:45 PM (14 years, 4 months ago)

There's no need. It's not an experiment, it's just goofing around with spores. You've got spores, give it a try. :shrug:


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Invisibletruskool
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Registered: 06/28/09
Posts: 11,194
Loc: Over the rainbow
Re: Inoculating a Jar with Several Different Sub-strains [Re: Florida]
    #11418693 - 11/09/09 12:46 PM (14 years, 4 months ago)

I am willing to do it right now.  I got some jars of rye ready and have been deciding what to inoculate with


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OfflineFlorida
Soul Rebel


Registered: 04/30/09
Posts: 149
Loc: Florida
Last seen: 11 years, 9 months
Re: Inoculating a Jar with Several Different Sub-strains [Re: Shea25]
    #11418745 - 11/09/09 12:53 PM (14 years, 4 months ago)

Quote:

Shea25 said:
There would be too much moisture and a better chance of it contaming



True, but your missing the point here. What if the spore to substrate ratios were proportional to a single strain inoculation?


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Invisibletruskool
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Re: Inoculating a Jar with Several Different Sub-strains [Re: Florida]
    #11418762 - 11/09/09 12:54 PM (14 years, 4 months ago)

ima put like 2-3 drops of each into a jar


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Ask  AMU    for    the  best    mycology    advice  out  there

  Roll it :burnone:While I troll it.  :maximumtrolling:

I don't mean to boast, but dam if I don't brag.

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OfflineFlorida
Soul Rebel


Registered: 04/30/09
Posts: 149
Loc: Florida
Last seen: 11 years, 9 months
Re: Inoculating a Jar with Several Different Sub-strains [Re: truskool]
    #11418765 - 11/09/09 12:55 PM (14 years, 4 months ago)

Awesome. If you are serious! Make a thread I'm sure a lot of people would be interested.:smile::thumbup::mushroom2:


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Invisibletruskool
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Registered: 06/28/09
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Re: Inoculating a Jar with Several Different Sub-strains [Re: Florida]
    #11418772 - 11/09/09 12:55 PM (14 years, 4 months ago)

Yea ill throw it up in a couple hours


--------------------
Ask  AMU    for    the  best    mycology    advice  out  there

  Roll it :burnone:While I troll it.  :maximumtrolling:

I don't mean to boast, but dam if I don't brag.

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Invisiblenachohippie
asshole
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Registered: 09/19/09
Posts: 2,623
Loc: right around the corner Flag
Re: Inoculating a Jar with Several Different Sub-strains [Re: feelfunny]
    #11418778 - 11/09/09 12:56 PM (14 years, 4 months ago)

Quote:

feelfunny said:
the one that will win will just be the strongest of the samples and will probly  be different every time.



sounds about right to me


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OfflineFlorida
Soul Rebel


Registered: 04/30/09
Posts: 149
Loc: Florida
Last seen: 11 years, 9 months
Re: Inoculating a Jar with Several Different Sub-strains [Re: truskool]
    #11418786 - 11/09/09 12:57 PM (14 years, 4 months ago)

Quote:

truskool said:
Yea ill throw it up in a couple hours



I changed my mood.  :grin:


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Invisibletruskool
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Registered: 06/28/09
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Re: Inoculating a Jar with Several Different Sub-strains [Re: Florida]
    #11418805 - 11/09/09 12:59 PM (14 years, 4 months ago)

Yea i doubt anyone will be able to tell the difference of any of the fruits I am cooking up a bunch of jars and thought that it would be fun to try one jar like this


--------------------
Ask  AMU    for    the  best    mycology    advice  out  there

  Roll it :burnone:While I troll it.  :maximumtrolling:

I don't mean to boast, but dam if I don't brag.

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Offlineapoonanor
Time & Space Traveler


Registered: 04/20/09
Posts: 380
Last seen: 9 years, 10 months
Re: Inoculating a Jar with Several Different Sub-strains [Re: truskool]
    #11420666 - 11/09/09 05:43 PM (14 years, 4 months ago)

inoculating jars with multiple syringes will most likely produce generic looking cubes that will most likely act like generic looking cubes. given that they are all multispore inoculations, your results will look like cubes, act like cubes, and behave like...well, cubes.

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Invisibletruskool
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Re: Inoculating a Jar with Several Different Sub-strains [Re: apoonanor]
    #11420690 - 11/09/09 05:46 PM (14 years, 4 months ago)



--------------------
Ask  AMU    for    the  best    mycology    advice  out  there

  Roll it :burnone:While I troll it.  :maximumtrolling:

I don't mean to boast, but dam if I don't brag.

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