Home | Community | Message Board


Vaposhop
Please support our sponsors.

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Original Seeds Store Buy Cannabis Seeds, Buy CBD   Amazon ½ Pint Jars, Agar

Jump to first unread post. Pages: 1
Invisibletruskool
WTF?? FTW!!
Male User Gallery


Registered: 06/28/09
Posts: 11,194
Loc: Over the rainbow
Saving Lizard King
    #11418412 - 11/09/09 02:11 PM (8 years, 10 months ago)



So I thought these jars were contaminated and I place them on my veranda until I cleaned them out.  So today I decided to stop being lazy and started cleaning jars out and to my surprise found this jar invitro pinning.  It is Lizard King and I lost the original print.  I would really like to save this race for my collection.  I was thinking that maybe I could dissect one of the shrooms soak it in peroxide and place it onto agar to get an isolate.  I am correct in this?  How long should I soak it in H2O2?  Where is the best place to dissect?  Inside the stem?  The cap?  For the proper instructions I am willing to give a Lizard King print out to those that help me once I complete a successful grow.


--------------------
Ask  AMU    for    the  best    mycology    advice  out  there

  Roll it :burnone:While I troll it.  :maximumtrolling:

I don't mean to boast, but dam if I don't brag.


Post Extras: Print Post  Remind Me! Notify Moderator
Invisiblefeelfunny
I am you
Male User Gallery


Folding@home Statistics
Registered: 03/11/09
Posts: 8,747
Loc: South
Re: Saving Lizard King [Re: truskool]
    #11418540 - 11/09/09 02:27 PM (8 years, 10 months ago)

just put that on agar. no need to put in H2O2 just cut from the base or from right under the cap on the stem.


--------------------
IF A CAT AND DOG CAN GET ALONG WHY CANT EVERYONE ELSE?
If the sky is falling, don't look up!  :abduction:

Feel Family Founder. :pm: me if you are tired of hearing, "Use the search function".


Post Extras: Print Post  Remind Me! Notify Moderator
Invisiblebadman
Male User Gallery

Registered: 06/14/06
Posts: 4,039
Re: Saving Lizard King [Re: truskool]
    #11418593 - 11/09/09 02:34 PM (8 years, 10 months ago)

Inside the stem is perfect, near the base is where i take my samples form. Peroxide should be fine also you can do two washes in H2O2, wash and then in some fresh peroxide soak for a while. I soak for less than 5 mins but im impatient, a lil longer is ok i wouldnt do it for 30 mins or something. Id use the H2O2 cos you know the jar is contamed otherwise no need.

The clone tissue may sector and further work may be needed to isolate the various sectors which could appear. Each sector should then be tested to see its productivity or what ever factor you like, size, potency....


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineBrandNewbie
Captain
Male


Registered: 05/21/08
Posts: 2,920
Loc: U.S.A.
Last seen: 3 years, 11 months
Re: Saving Lizard King [Re: truskool]
    #11418625 - 11/09/09 02:38 PM (8 years, 10 months ago)

At the top of the stipe right below the cap is the recommended place to harvest a tissue sample.

No need to use H2O2. Just slice off the outer skin and use a syringe to collect the sample from the inside of the stipe.

I hope you can save it! :goodluck:


--------------------
Question: Why do women wear make-up and perfume? 
Answer: Because they're ugly and they stink.


Post Extras: Print Post  Remind Me! Notify Moderator
Invisibletruskool
WTF?? FTW!!
Male User Gallery


Registered: 06/28/09
Posts: 11,194
Loc: Over the rainbow
Re: Saving Lizard King [Re: BrandNewbie]
    #11418671 - 11/09/09 02:44 PM (8 years, 10 months ago)

very cool.  The jar is from september I forgot to mention is that a problem?  FF, badman, and newbie once I get some fruits ill be sure to send you a print each


--------------------
Ask  AMU    for    the  best    mycology    advice  out  there

  Roll it :burnone:While I troll it.  :maximumtrolling:

I don't mean to boast, but dam if I don't brag.


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineBrandNewbie
Captain
Male


Registered: 05/21/08
Posts: 2,920
Loc: U.S.A.
Last seen: 3 years, 11 months
Re: Saving Lizard King [Re: truskool]
    #11418697 - 11/09/09 02:47 PM (8 years, 10 months ago)

It doesn't matter how long it's been out there.  As long as the shrooms are still alive, your golden.

Remember, it's just as easy to do four dishes as it is to do one... :wink:


--------------------
Question: Why do women wear make-up and perfume? 
Answer: Because they're ugly and they stink.


Post Extras: Print Post  Remind Me! Notify Moderator
Invisibletruskool
WTF?? FTW!!
Male User Gallery


Registered: 06/28/09
Posts: 11,194
Loc: Over the rainbow
Re: Saving Lizard King [Re: BrandNewbie]
    #11418730 - 11/09/09 02:51 PM (8 years, 10 months ago)

Quote:

BrandNewbie said:
It doesn't matter how long it's been out there.  As long as the shrooms are still alive, your golden.

Remember, it's just as easy to do four dishes as it is to do one... :wink:



Are they still alive?  Four dishes instead of one?  I take it you mean to use four samples and do four dishes of agar?


--------------------
Ask  AMU    for    the  best    mycology    advice  out  there

  Roll it :burnone:While I troll it.  :maximumtrolling:

I don't mean to boast, but dam if I don't brag.


Post Extras: Print Post  Remind Me! Notify Moderator
Invisiblefeelfunny
I am you
Male User Gallery


Folding@home Statistics
Registered: 03/11/09
Posts: 8,747
Loc: South
Re: Saving Lizard King [Re: truskool]
    #11418758 - 11/09/09 02:54 PM (8 years, 10 months ago)

yes and i have a print i just put on agare sunday but thankk you


--------------------
IF A CAT AND DOG CAN GET ALONG WHY CANT EVERYONE ELSE?
If the sky is falling, don't look up!  :abduction:

Feel Family Founder. :pm: me if you are tired of hearing, "Use the search function".


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineBrandNewbie
Captain
Male


Registered: 05/21/08
Posts: 2,920
Loc: U.S.A.
Last seen: 3 years, 11 months
Re: Saving Lizard King [Re: truskool]
    #11418790 - 11/09/09 02:58 PM (8 years, 10 months ago)

Yeah, sorry.  I meant four agar plates.  Just to be on the safe side.

That way if you grow the green, you'll still have a better chance of a clean isolate. :thumbup:


--------------------
Question: Why do women wear make-up and perfume? 
Answer: Because they're ugly and they stink.


Post Extras: Print Post  Remind Me! Notify Moderator
Invisibletruskool
WTF?? FTW!!
Male User Gallery


Registered: 06/28/09
Posts: 11,194
Loc: Over the rainbow
Re: Saving Lizard King [Re: BrandNewbie]
    #11418859 - 11/09/09 03:06 PM (8 years, 10 months ago)

Any chance of casing this jar and fruiting it?


--------------------
Ask  AMU    for    the  best    mycology    advice  out  there

  Roll it :burnone:While I troll it.  :maximumtrolling:

I don't mean to boast, but dam if I don't brag.


Post Extras: Print Post  Remind Me! Notify Moderator
Invisibleprismism
 User Gallery


Registered: 05/11/09
Posts: 5,566
Re: Saving Lizard King [Re: truskool]
    #11418874 - 11/09/09 03:08 PM (8 years, 10 months ago)

whats the story behind this 'lizard king' variety?


--------------------
ephemeral anomalous


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineBrandNewbie
Captain
Male


Registered: 05/21/08
Posts: 2,920
Loc: U.S.A.
Last seen: 3 years, 11 months
Re: Saving Lizard King [Re: truskool]
    #11418881 - 11/09/09 03:09 PM (8 years, 10 months ago)

Well you were going to toss it, yeah?  I say, if you don't mind the work, what are you out?  Some horse turds?GO FOR IT BRUDDA! :rockon:


--------------------
Question: Why do women wear make-up and perfume? 
Answer: Because they're ugly and they stink.


Post Extras: Print Post  Remind Me! Notify Moderator
Invisiblebadman
Male User Gallery

Registered: 06/14/06
Posts: 4,039
Re: Saving Lizard King [Re: BrandNewbie]
    #11419013 - 11/09/09 03:31 PM (8 years, 10 months ago)

Geeza, cheers for the print offer but you might as well give it to someone who will use it.


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineKanker
Hides in tall grass
Male


Registered: 07/16/07
Posts: 738
Loc: On a Long dead-heart.
Last seen: 7 years, 4 months
Re: Saving Lizard King [Re: prismism]
    #11423131 - 11/10/09 12:53 AM (8 years, 10 months ago)

believe it was a texas cube, which carries the name of the 'founder' (a member on the forums) was found to have partially colonized wood chips as well.  it's in the strain guide though.
Godwilling, there should be some prints up for grabs in the next 2 weeks  :bow2:


--------------------
I'm ahead, I'm advanced
I am the first mammal to make plans, yeah
I crawled the earth, but now I'm higher
2010, watch it go to fire.
It's evolution baby.
-Pearl Jam


Post Extras: Print Post  Remind Me! Notify Moderator
Invisible13shroomsM
Lightning Shaman
I'm a teapot User Gallery


Registered: 01/01/09
Posts: 26,154
Loc: IN ETHERS TORSION FIELD
Trusted Cultivator
Re: Saving Lizard King [Re: Kanker]
    #11423336 - 11/10/09 01:33 AM (8 years, 10 months ago)

if you have 2qts of LKs spawn get your tissue samples that you want then spawn them to 4qts of CVC+g (mix is up 2 U) in a tray/minimono. 
Id fruit it away from all your other projects incase the contams come back. :stoned:


--------------------
:takingnotes:A M U:helpdesk:
Click here ^ for the AMU forum
VVV AMUs Free Active/Edible/Exotic Spore Print or Syringe or Edible Culture Trade Thread VVV
"Man is the sex organ of the machine world" ~  Marshall McLuhan 


Post Extras: Print Post  Remind Me! Notify Moderator
Jump to top. Pages: 1

Shop: Original Seeds Store Buy Cannabis Seeds, Buy CBD   Amazon ½ Pint Jars, Agar

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

Similar ThreadsPosterViewsRepliesLast post
* Re: Lizard King --Need you opinion on casing please Lizard King 1,205 4 07/11/00 01:43 PM
by Anonymous
* Re: Thanks to Lizard King..... Lizard King 1,249 2 08/16/00 07:27 AM
by CLuB99
* Re: Lizard King... a fluke? camel 867 4 04/27/00 10:54 AM
by phrozendata
* Lizard King Russdogg 1,420 18 04/04/01 04:46 PM
by Thor
* Re: Alien substrate help..Lizard king or some one(Finch Seed?) Lizard King 1,021 1 06/28/00 12:51 AM
by Ru
* Mutations due to H2O2 (peroxide?) KlingonFromUranus 1,749 8 11/13/03 05:01 AM
by plura16
* hydrogen peroxide? Yaniphc 1,169 6 11/09/01 07:38 PM
by Yaniphc
* h2o2? criminulmindid 531 1 11/25/01 04:47 PM
by Shdwstr

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Roadkill, Magash, Shroomism, george castanza, RogerRabbit, Citric, total, FooMan, 13shrooms, stonesun, cronicr, PussyFart, Tmethyl, Pastywhyte, bodhisatta
1,670 topic views. 28 members, 114 guests and 15 web crawlers are browsing this forum.
[ Toggle Favorite | Print Topic | Stats ]
Search this thread:
Shroom Supply
Please support our sponsors.

Copyright 1997-2018 Mind Media. Some rights reserved.

Generated in 0.039 seconds spending 0.009 seconds on 19 queries.