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OfflineN0rthernLights
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Registered: 10/14/09
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another needle sterlization question
    #11414381 - 11/08/09 11:19 PM (7 years, 11 months ago)

Hello all. Let me first start by saying this site is awesome. I am thoroughly impressed by the wealth of knowledge within the community here. I have spent the last few months lurking around trying to soak up as much knowledge as possible. Now I am actually gathering my supplies and starting to get ready for my first grow. Pretty exciting huh?

Well on to my question, and yes I did search, but would appreciate some clarification.

Obviously the idea of a glove box is to eliminate the chance for contamination. Same with self sealing lids. This has prompted me to start building both. My glove box is simple; plastic container with 4 inch holes for arms, 4 inch plastic caps for when not in use, plexi glass lid, and disposable sleeves/gloves. My concern however is sterilizing the needle between injections.

It seems if I were to apply a generous amount of alcohol in sterilizing the glove box, using a torch lighter inside to sterilize my needle could be at the least slightly dangerous.

So my alternative is then to do this part outside the glove box. However, does this not defeat the whole purpose of the self sealing lids and the glove box? It seems pulling my hands in and out of the glove box between each flaming of the needle would be synonymous with introducing contaminants to my work space?

I am very interested in hearing how you guys combat this issue.

Thanks in advance.


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InvisibleDoc_T
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Re: another needle sterlization question [Re: N0rthernLights]
    #11414415 - 11/08/09 11:22 PM (7 years, 11 months ago)

Don't use alcohol to clean the box, there's no need. Plain soapy water will do it.
Then you can flame sterilize all you want inside the box.  Use an alc lamp or a 'crack lighter'.


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OfflineRazzleCap
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Re: another needle sterlization question [Re: Doc_T]
    #11414455 - 11/08/09 11:26 PM (7 years, 11 months ago)

Honestly, you don't even need a torch lighter. A Bic will work just fine. As for the glovebox, you can simply turn your bathroom into a sterile workspace buy wiping it down and disinfecting with lysol or something. Make sure to keep the room free of ventilation though.


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"If the words 'life, liberty, and the pursuit of happiness' don't include the right to experiment with your own consciousness, then the Declaration of Independence isn't worth the hemp it was written on."

"Let us declare nature to be legitimate. All plants should be declared legal, and all animals for that matter. The notion of illegal plants and animals is obnoxious and ridiculous."


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Re: another needle sterlization question [Re: RazzleCap]
    #11414515 - 11/08/09 11:36 PM (7 years, 11 months ago)

Quote:

RazzleCap said:
you can simply turn your bathroom into a sterile workspace




False.
Agreed about the lighter, a Bic will work ok.


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OfflineDamion5050
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Re: another needle sterlization question [Re: Doc_T]
    #11414535 - 11/08/09 11:37 PM (7 years, 11 months ago)

I also disagree the bathroom has the most bacteria per square inch than anywhere else in a house.

Especially the toilet and bathroom sink.


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Offlinexbrutalx233
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Re: another needle sterlization question [Re: Doc_T]
    #11414543 - 11/08/09 11:39 PM (7 years, 11 months ago)

Well, what you should do, is use the sponge trick, just soak a sponge in alcohol, flame the needle, quickly slide the sponge over needle and inoculate without ever letting the needle be exposed to open air, I've had about 8 out of 9 jar success rate thus far, the one that failed I think was the first one I did before I got the whole thing down...


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Re: another needle sterlization question [Re: Damion5050]
    #11414553 - 11/08/09 11:40 PM (7 years, 11 months ago)

Kitchen vs bathroom, dunno.
Either vs glovebox, no comparison.

I work in open air on my stove. I don't use a glovebox to shoot up jars.
If it's your first time, or if you have high contam rates, you probably should use a glovebox. But you don't have to.


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Offlineskullhuman
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Re: another needle sterlization question [Re: Doc_T]
    #11414570 - 11/08/09 11:42 PM (7 years, 11 months ago)

I never use a glove box for shooting jars either.  Don't think there's any need to use alcohol if you've already flame sterilized either.


Edited by skullhuman (11/08/09 11:43 PM)


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Re: another needle sterlization question [Re: skullhuman]
    #11414582 - 11/08/09 11:44 PM (7 years, 11 months ago)

Agreed also. Flame or alc on the needle, no need for both. And I prefer flame.


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OfflineDamion5050
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Re: another needle sterlization question [Re: skullhuman]
    #11414585 - 11/08/09 11:44 PM (7 years, 11 months ago)

Quote:

skullhuman said:
I never use a glove box for shooting jars either.  Don't think there's any need to use alcohol if you've already flame sterilized either.  If anything I suspect it might be counter-productive.





Agree if you are using a flame it should kill everything on and in the needle..  But I always do all my work in front of my flowhood.  Just my personal preference..


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Invisibledancefloordale
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Re: another needle sterlization question [Re: Damion5050]
    #11414654 - 11/08/09 11:52 PM (7 years, 11 months ago)

Flame it and stick it straight through your injection port that you have wiped with alcohol.

No need to cool the needle, just jab it through. If you heated it up red hot all contams are killed and anything landing on it from the time it is flamed to the time it goes into the port will be killed as well. Should take no more than a second.

Be careful with having an alcohol lamp in the GB. It may melt a hole in the top. Secondly, the flame creates air currents in a GB, and we want still air.


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Everything posted by the user dancefloordale, aside from what is written in this here clause is completely and utterly fictitious, despite any information read (or seen), above (or below) that might lead you to believe otherwise.

Hydra Tek - A detailed guide, for newest to the most skilled cultivators.

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Invisibledaytripper05
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Re: another needle sterlization question [Re: dancefloordale]
    #11415046 - 11/09/09 12:38 AM (7 years, 11 months ago)

Yeah my needle is still red hot after I get in the GB. No need to sterilize inside the glovebox which can be dangerous.


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OfflineN0rthernLights
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Re: another needle sterlization question [Re: daytripper05]
    #11416053 - 11/09/09 03:16 AM (7 years, 11 months ago)

Cool, I appreciate everyone's input. Sounds like I was being a little over cautious.

So do you think if I put my sterilized jars and flamed needle strait into a clean glove box, I could then inject multiple jars safely by  simply wiping the needle and injection ports with alcohol between each jar?


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Invisibledancefloordale
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Re: another needle sterlization question [Re: N0rthernLights]
    #11416200 - 11/09/09 03:41 AM (7 years, 11 months ago)

Probably, but I would do it between each jar, just in case.


--------------------
Everything posted by the user dancefloordale, aside from what is written in this here clause is completely and utterly fictitious, despite any information read (or seen), above (or below) that might lead you to believe otherwise.

Hydra Tek - A detailed guide, for newest to the most skilled cultivators.

HCA

Bulk growing made easy-discussion
Bulk Growing Made Easy


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OfflineMissShroom
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Re: another needle sterlization question [Re: N0rthernLights]
    #11416497 - 11/09/09 05:04 AM (7 years, 11 months ago)

I would advice you to flame the needle between each jar. Otherwise if one of them somehow got contaminated then all of them will get. That's a small thing to do it can save you from big troubles.


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OfflineThyrax
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Re: another needle sterlization question [Re: MissShroom]
    #11416910 - 11/09/09 07:40 AM (7 years, 11 months ago)

dude remove those glove from your glove box, no need for them. And then you will have no prob sterilizing your needle.


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OfflineN0rthernLights
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Re: another needle sterlization question [Re: Thyrax]
    #11417765 - 11/09/09 12:20 PM (7 years, 11 months ago)

Haha damn.. Now I am confused again.

method 1)
I PC my jars and syringe, flame needle, then load all into GB(along with some alcohol). Then wipe needle/inject-port with alcohol and inject. Then wipe down needle and next jar and inject that one.... etc

method 2)
I PC my jars and syringe, flame needle, then load all into GB (along with some alcohol). Then wipe needle/inject-port with alcohol and inject. Then pull arms out of GB, re flame needle, and then put arms back in GB where I again wipe needle/inject-port with alcohol and inject...etc

Is the general consensus that with method 2, even though I am allowing more contaminants the chance to enter my GB I am still safer due to the fact that I flamed my needle between each injection?


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Invisiblemyceleus_rex
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Re: another needle sterlization question [Re: N0rthernLights]
    #11417811 - 11/09/09 12:30 PM (7 years, 11 months ago)

Quote:

N0rthernLights said:
I PC my jars and syringe,




Your not thinking that you should PC a spore syringe... are you?


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Cervantes' sclerotia thread    Citric's self-healing lid tek  Agar's Grain LC  Breaking Up Spawn Jars

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OfflineN0rthernLights
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Re: another needle sterlization question [Re: myceleus_rex]
    #11419074 - 11/09/09 03:41 PM (7 years, 11 months ago)

myceleus_rex

Actually I was..
Yes that does seem like it would kill the spores but, I could have sworn I saw a tek showing the spore syringe wrapped in tinfoil and then PCed along with jars? If I am completely wrong in this thanks for catching my error before I ruined any syringes.

Could you please answer the question regarding the 2 methods assuming I were to not PC the syringe and only flame the needle before the initial entering into the GB?


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Invisiblemyceleus_rex
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Re: another needle sterlization question [Re: N0rthernLights]
    #11419252 - 11/09/09 04:07 PM (7 years, 11 months ago)

Quote:

N0rthernLights said:
myceleus_rex

Actually I was..
Yes that does seem like it would kill the spores but, I could have sworn I saw a tek showing the spore syringe wrapped in tinfoil and then PCed along with jars? If I am completely wrong in this thanks for catching my error before I ruined any syringes.

Could you please answer the question regarding the 2 methods assuming I were to not PC the syringe and only flame the needle before the initial entering into the GB?



Glad I caught it to :grin:
DO NOT PC the spore syringe...
NO NO NO  :nono:
Go with method 2. Flame each time and wipe the port. No need to wipe needle after flaming for each jar.

PCing syringes is for re-using them, like prior to making LC or making spore syinges from a print.


--------------------
Cervantes' sclerotia thread    Citric's self-healing lid tek  Agar's Grain LC  Breaking Up Spawn Jars

World's Ugliest Cat?

If ignorance is bliss, why aren't there more happy people?


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