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Invisibledrainhaven
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Pretty Basic LC Questions
    #11412287 - 11/08/09 06:24 PM (7 years, 11 months ago)

Three Part Question:

1)I want to make an easy LC.  Any tips/tricks on taking a colonized WBS grain and dropping it in sterilized water? I tried it once but it took a long time to grow out. I sterilized some eyebrow tweezers and grabbed one and dropped it in. I havent had great luck dropping agar into it.

2)Also, can I drop a few cc's of MS into sterilzed water for an LC? I guess what Im asking is will myc grow or is it so diluted its a waste. 

3)LC's need a hole for gas exchange?
I appreciate it...


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OfflineDamion5050
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Re: Pretty Basic LC Questions [Re: drainhaven]
    #11412307 - 11/08/09 06:27 PM (7 years, 11 months ago)



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Invisiblebotanisthype
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Re: Pretty Basic LC Questions [Re: drainhaven]
    #11412318 - 11/08/09 06:29 PM (7 years, 11 months ago)

1-just take the myceliel tissue and drop that in

2-no it will not germinate becuz it has no nutrients to thrive upon

3-no...but it helps ...the vacuum suck..if you have no extra hole ..the vacuum suck will suck all your MS out in about 1-second


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Invisibledrainhaven
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Re: Pretty Basic LC Questions [Re: botanisthype]
    #11412337 - 11/08/09 06:32 PM (7 years, 11 months ago)

Damion, thanks for the links. I have those links, Grain LC water especially, but I am hoping this way is the easiest/safest bet.

1)So the grain is a waste, maybe just scrape some off somewhere?

2)So that is why honey/karo is added? I guess once it is myc it isn't necessary?

3)Vacuum suck?


Edited by drainhaven (11/08/09 06:34 PM)


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OfflineKanker
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Re: Pretty Basic LC Questions [Re: drainhaven]
    #11412443 - 11/08/09 06:49 PM (7 years, 11 months ago)

2) the additive other than water is the nutrient base for it to grow upon, once this is fully consumed by the myc (1-2 weeks @ 4%/100mL?) you'll want to refrigerate it, which can be kept for months.

3) by creating a seal (no air holes) you've essentially got a vaccum, (assuming you PC it, not sure if steam sterilization does the same thing) and if you were to inject your MS / mycelium via injection, the vaccum will suck the syringe out whether you want to or not.


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Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

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