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Offlinedankster
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question on Lid for LC
    #11408804 - 11/08/09 02:16 AM (7 years, 11 months ago)

I just made a simple self-healing lid for a LC.  It is a normal metal caning lid with a 1/4" hole drilled in it and a big glob of silicone covering the whole thing. 

Should I put the lid on rubber side up or down when I PC it?  I was thinking if I put it down, the rubber could seal and then when the jar cools and pressure drops, the silicone could get sucked through the hole.  If I put it up, I wasnt sure if there would be a good enough seal to keep bacteria out. 

Also, when I suck the LC up into the syringe, , I was planning on making up for the pressure difference caused by its removal by pumping steril air in with a syringe first.  I would get the sterile air by sticking the needle in a flame and pulling the plunger out.  Is this a reliable way to get sterile air? 

What do you think about this system?


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Offlinecyb3rtr0n
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Re: question on Lid for LC [Re: dankster]
    #11408857 - 11/08/09 02:33 AM (7 years, 11 months ago)

Another option would be to make another injection port. Use the second RTV port as a air-port.  A syringe stuffed with poly-fil, and the plunger removed (flame sterilize the needle).  you could insert that into the jar, wait a couple seconds; then put your spore syringe in.


I use rubber side down, like normal.. the lids are designed to allow air exchange while being sterilized.

The only problem you may run into is boilover. let the pc cool all the way down to 0 or 1 psi, before releasing the valve.

here:

Quote:


http://www.shroomery.org/forums/showflat.php/Number/6065011#6065011
Correct. That's what causes agar to boil over as well. I use extra water in the PC when doing agar or LC so the pressure will drop more slowly due to the thermal mass of the extra water. Sometimes it also helps to reduce the stove burner slowly over 20 minutes rather than simply shutting it off after time expires. As said above never try to speed up the process by lifting the weight or doing anything else to cool off the PC.

The other thing that causes boil over is too high a stove setting during the PC cycle. If the weight is rattling, LC is boiling over. You need to reduce the heat to the minimum setting that will maintain the 15 psi as soon as pressure is reached. Don't allow the weight to rattle.
RR




Edited by cyb3rtr0n (11/08/09 02:40 AM)


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OfflineSpongiform
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Re: question on Lid for LC [Re: cyb3rtr0n]
    #11408874 - 11/08/09 02:37 AM (7 years, 11 months ago)

When I did that, my LC would grow a small amount then stall out due to lack of gas exchange.  I 'fixed' it by injecting sterilized air.  After that I started using tyvek filter patches for gas exchange.

I make all my jars (for grains & lc like this).



Works fine.  Have to replace the tyvek after a couple uses though.


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OfflineMikeallojee
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Re: question on Lid for LC [Re: Spongiform]
    #11607257 - 12/08/09 12:12 AM (7 years, 10 months ago)

Where do you get your plungers?


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Invisiblenoobieshroomie
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Re: question on Lid for LC [Re: Mikeallojee]
    #11607281 - 12/08/09 12:17 AM (7 years, 10 months ago)

all the info you need is here
http://www.shroomery.org/forums/showflat.php/Number/11487687

  -noobie-


--------------------
AMU

Best Thread Ever
CapZilla said:
not sure what GE and FAE are but i should probably get some.

Citric said:
Your signature is wrong on colonization temps!

GOOD JUDGMENT COMES FROM EXPERIENCE
EXPERIENCE COMES FROM BAD JUDGMENT

ROOM TEMP 70-75 IS BEST FOR COLONIZATION
Thank you mycochef


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Offlinefig
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Re: question on Lid for LC [Re: noobieshroomie]
    #11607306 - 12/08/09 12:20 AM (7 years, 10 months ago)

gettem noobieshroomie lol


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Amazon Shop: ½ Pint Jars, Agar

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