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Amazon Shop: ½ Pint Jars, Pressure Cooker, Wild Bird Seed

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Invisibleother one
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Registered: 11/06/09
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problems with grain to grain transfers
    #11400895 - 11/06/09 10:50 PM (7 years, 11 months ago)

ive been learning about cultivation on shroomery for about
a year or more now, it is today i decided to create an account.
i have been experiencing problems with doing grain to grain transfers with wild bird seed. i used two cubensis strains, nepals, and ecuadors.
the nepals are doing fine. the ecuadors however seem to stop colonizing after i do my first transfer from a jar that was inoculated with a spore syringe. i have heard of something called senescence which i think has something to do with the breaking down of mycelium after a few tranfers but my research hasnt turned up a full explanation. my question is should i just stop doing transfers as they are useless with the ecuadors and instead use spores or is there something else im missing?


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Invisibleavorg
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Re: problems with grain to grain transfers [Re: other one]
    #11400903 - 11/06/09 10:52 PM (7 years, 11 months ago)

it is highly unlikely that you will experience senescence with only one transfer. it may be moisture content problems, one variety may like your conditions, and the other may not.


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Invisibleother one
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Re: problems with grain to grain transfers [Re: other one]
    #11400938 - 11/06/09 10:59 PM (7 years, 11 months ago)

well i have a 10 p.s.i. pressure cooker and havent experienced problems with contamination however the birdseeds seem to have a burnt appearenced after pressure cooking. i have considered injecting water into the jars but havent had a chance to try it yet. but incorrect moisture does sound like the problem considerinh their burnt appearence


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Re: problems with grain to grain transfers [Re: other one]
    #11400959 - 11/06/09 11:04 PM (7 years, 11 months ago)

how long are you cooking?

does the water ever run out in your PC?

and contams favor higher water content IME, so this might make sense.

also you might be okay, but as far as I know all Teks are designed for 15psi.


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InvisibleShea25
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Re: problems with grain to grain transfers [Re: other one]
    #11400969 - 11/06/09 11:07 PM (7 years, 11 months ago)

10 PSI is fine just PC them for 2 hours


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Re: problems with grain to grain transfers [Re: avorg]
    #11400975 - 11/06/09 11:08 PM (7 years, 11 months ago)

i have cooked them for one hour and they looked burnt.
i decreased sterilization time to thirty min. and even 25 min.
but still burnt. now that you mention it, yes water does run out of the pc. it like drips out of the handle and other parts at random times


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InvisibleShea25
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Re: problems with grain to grain transfers [Re: other one]
    #11400990 - 11/06/09 11:11 PM (7 years, 11 months ago)

keep the jars propped up off the bottom of the PC. Add a few inches of water(you dont want the jars to be sitting in water). Turn the stove on max wait for the rocker on top of the Pc to rock start your timer for 2 hours now(thats if your only at 10 PSI) now turn the stove down to low just keeping the rocker rocking.

At 15 PSI always PC for 90 minutes
At 10 PSI always PC for 120 minutes


Edited by Shea25 (11/06/09 11:11 PM)


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Re: problems with grain to grain transfers [Re: Shea25]
    #11401007 - 11/06/09 11:15 PM (7 years, 11 months ago)

ok i will fold the cloth to make it more thick.
but another thing is when i turn it to max and wait for the
rocker to rock, once it does and i turn it to low the rock eventually stops rocking and steam slowly vents out of it. perhaps you have a gas stove where as mines electric but i just figured one the pressures in there i shrould be alright.


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Re: problems with grain to grain transfers [Re: other one]
    #11401020 - 11/06/09 11:18 PM (7 years, 11 months ago)

Quote:

other one said:
i have cooked them for one hour and they looked burnt.
i decreased sterilization time to thirty min. and even 25 min.
but still burnt. now that you mention it, yes water does run out of the pc. it like drips out of the handle and other parts at random times




sounds like you have a sub-par PC, is it Mirro brand by any chance?

water leaking is not good, but what i was really referring to was if there was any water left in the PC after your cooking was done, when you take the jars out.


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Re: problems with grain to grain transfers [Re: other one]
    #11401025 - 11/06/09 11:21 PM (7 years, 11 months ago)

Quote:

other one said:
ok i will fold the cloth to make it more thick.
but another thing is when i turn it to max and wait for the
rocker to rock, once it does and i turn it to low the rock eventually stops rocking and steam slowly vents out of it. perhaps you have a gas stove where as mines electric but i just figured one the pressures in there i shrould be alright.




not true you need the water to keep boiling to keep the pressure at that level. I too have an electric stove, and I keep it on high then lower to a notch below medium-slightly above medium.


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Re: problems with grain to grain transfers [Re: avorg]
    #11401029 - 11/06/09 11:21 PM (7 years, 11 months ago)

yes it is a mirro brand pc. i would like to upgrade to a 15 p.s.i
but the only reoson i have this one was because i met a generous cultivator one time. yes there is water left in the pc when its done
as i read that if it goes dry thats a bad thing


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Re: problems with grain to grain transfers [Re: other one]
    #11401040 - 11/06/09 11:24 PM (7 years, 11 months ago)

i thought that it was supposed to keep rocking. i will be sure to correct these problems and keep in on medium. but it really sounds like i need a new pressure cooker. but i dont know where im goint to get seventy bucks these days


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Re: problems with grain to grain transfers [Re: other one]
    #11401049 - 11/06/09 11:27 PM (7 years, 11 months ago)

go to your local thrift shops around town and pick one up for 5 bucks. Just make sure to give it a good look over to make sure its in good shape


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Re: problems with grain to grain transfers [Re: Shea25]
    #11401060 - 11/06/09 11:29 PM (7 years, 11 months ago)

sounds like a plan. never thought of that but im going to get right on it. thanks for the tip:mushroom2:


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Re: problems with grain to grain transfers [Re: other one]
    #11401077 - 11/06/09 11:32 PM (7 years, 11 months ago)

so, grain to grains do work like i thought and i could take one quart jar and make twenty. cause the idea of taking a load of spore prints and making syringes is way to hard when i could just do it once in a while and just make a glovebox and do transfers.


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Re: problems with grain to grain transfers [Re: other one]
    #11401084 - 11/06/09 11:33 PM (7 years, 11 months ago)

or well not twenty but like ten


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Re: problems with grain to grain transfers [Re: other one]
    #11401089 - 11/06/09 11:35 PM (7 years, 11 months ago)

now that all that is out of the way, I don't think this is the root of your problem.

how are you preparing your WBS?


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Re: problems with grain to grain transfers [Re: other one]
    #11401093 - 11/06/09 11:36 PM (7 years, 11 months ago)

It's possible to colonize a gallon jar, that should be able to knock up 40 quart jars.

:awesome:


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Re: problems with grain to grain transfers [Re: avorg]
    #11401109 - 11/06/09 11:39 PM (7 years, 11 months ago)

i follow docs wild birdseed step by step. strain, rinse, soak over night till it has the fermentation smell coming from it, rinse again, heat them up till steam is rising for twenty five minutes without too many exploding kernals, rinse, and i threw out the idea of letting them dry in the strainer cause of the whole burnt thing, and then i load into jar, apply tyvek, if im using a syringe i use the poly fill
and then pc.


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Re: problems with grain to grain transfers [Re: other one]
    #11401142 - 11/06/09 11:46 PM (7 years, 11 months ago)

Quote:

other one said:
heat them up till steam is rising for twenty five minutes without too many exploding kernals




this is where I think you are going wrong. you are supposed to simmer them in water to achieve correct moisture content. sounds like you are just cooking them.

is this right?


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Amazon Shop: ½ Pint Jars, Pressure Cooker, Wild Bird Seed

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

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