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Amazon Shop: Agar, Parafilm, Petri Dish

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Offlinecyb3rtr0n
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How many sectors do you start with?
    #11393218 - 11/05/09 08:58 PM (8 years, 8 months ago)

In working to isolate strains, how many multispore transfers do you make?

This was my first time working from a print on agar.  I now realised I used tons more spores than I actually needed, so my mycelium is pretty fluffy with layers of strains(i think).

I transferred from print to agar on 4 separate dishes, because I wasnt sure if thaey would all take. 

Now, I have 4 separate dishes, and I'm wondering if I should just start with one or teo transfers, because I'm thinking that I may not even see sectoring in the next transfer. 


I'm confused of how many  transfers I should cut out of these trays.

any suggestions?


btw: print was from The Greak Oakridge Giveaway.


Another question:  To those who use Parafilm; do you have problems with it tearing where the lid and base overlap on the petri?


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InvisibleCH HELL
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Re: How many sectors do you start with? [Re: cyb3rtr0n]
    #11393310 - 11/05/09 09:12 PM (8 years, 8 months ago)

Three or four transfer are good but it all depends on how far you want to go and how good you are at transferring small sectors.
CH


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Re: How many sectors do you start with? [Re: cyb3rtr0n]
    #11393360 - 11/05/09 09:21 PM (8 years, 8 months ago)

I would take two from each tray and keep going until I saw some rhizomorphic sectors. I think that it's really up to you how many more dishes you want to make from each of those.

I have yet to use agar to isolate, but I think that you could use the water dilution method to keep from having too much material to "clean" at such an early stage. It helps to shorten the road to a pure isolation that you want to keep alive.


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Re: How many sectors do you start with? [Re: CH HELL]
    #11393373 - 11/05/09 09:24 PM (8 years, 8 months ago)

I guess I'll find out how good I am at transferring small sectors when I do these..:lmafo:

3 or 4 sounds like a good starting point for transfers.  I think I like the bottom right one best.  maybe take two transfers from the 9-10 o'clock, and another from the 2to3 o'clock areas.  I think the top left plate is kinda neat, it resembles a mandelbrot; i definitely should transfer for that reason alone.



I want to isolate a few strains from this oakridge print, then grow them out on cakes or mini bread pans.  keep the best performers, then store it to a slant.  :shrug:


I'm ordering a case of 500 petris, but I'd like them to last a while.. right now I have about 40 petris on hand.


Edited by cyb3rtr0n (11/05/09 09:30 PM)


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Amazon Shop: Agar, Parafilm, Petri Dish

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