Home | Community | Message Board


Myco Supply
Please support our sponsors.

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Amazon Shop: Agar, Autoclave, Pressure Cooker

Jump to first unread post. Pages: 1
OfflinePfffffff
I am sofa king we todd ed

Registered: 10/02/09
Posts: 486
Loc: Somewhere that is fiction...
Last seen: 4 years, 4 months
Oven sterilizing tools
    #11378554 - 11/03/09 07:11 PM (7 years, 11 months ago)

Does anyone have a temperature/time guide for sterilizing tools in the oven?  I know it's only metal stuff that could be sterilized (duh) but it beats the hell out of a pressure cooker if you don't have any plastic items to sterilize...


--------------------
****************************************************
I am part of this community as a role playing
character.  All information is gathered on the
internet.  In no way, shape, or form is any of what
I say truthful or real life experience.  Any advice
given or received is purely for entertainment value
and not intended for any kind of illegal activity.
****************************************************


Post Extras: Print Post  Remind Me! Notify Moderator
InvisibleEpilson Lyrae
Armed with hammers
Male User Gallery


Registered: 04/07/09
Posts: 5,558
Loc: Woody Creek
Re: Oven sterilizing tools [Re: Pfffffff]
    #11378578 - 11/03/09 07:13 PM (7 years, 11 months ago)

what are you wanting to sterilize? For most things a good washing followed with some high % isopropyl alcohol will do the trick. That and flame sterilization of needles innoc loops etc.


--------------------
"Freedom is something that dies unless it's used." H.T.
I've come to believe that the heart is the filter of the enlightened mind. Epilson Lyrae


Post Extras: Print Post  Remind Me! Notify Moderator
OfflinePfffffff
I am sofa king we todd ed

Registered: 10/02/09
Posts: 486
Loc: Somewhere that is fiction...
Last seen: 4 years, 4 months
Re: Oven sterilizing tools [Re: Epilson Lyrae]
    #11378589 - 11/03/09 07:15 PM (7 years, 11 months ago)

I have a monstrous pair of tweezers I am in love with, and things like scalpels, pointy devices (I don't know wtf they're called like a needle with a handle you would poke at agar), etc...


--------------------
****************************************************
I am part of this community as a role playing
character.  All information is gathered on the
internet.  In no way, shape, or form is any of what
I say truthful or real life experience.  Any advice
given or received is purely for entertainment value
and not intended for any kind of illegal activity.
****************************************************


Post Extras: Print Post  Remind Me! Notify Moderator
InvisibleEpilson Lyrae
Armed with hammers
Male User Gallery


Registered: 04/07/09
Posts: 5,558
Loc: Woody Creek
Re: Oven sterilizing tools [Re: Pfffffff]
    #11378622 - 11/03/09 07:19 PM (7 years, 11 months ago)

I would just boil them and bag them up and wipe them down with alcohol before flame sterilizing the pointy bits at the time of use.


--------------------
"Freedom is something that dies unless it's used." H.T.
I've come to believe that the heart is the filter of the enlightened mind. Epilson Lyrae


Post Extras: Print Post  Remind Me! Notify Moderator
InvisiblenaumM
 User Gallery


Registered: 10/10/07
Posts: 3,774
Re: Oven sterilizing tools [Re: Pfffffff]
    #11379922 - 11/03/09 10:35 PM (7 years, 11 months ago)

In theory, you can dry sterilizer metal equipment in an oven, but it takes quite a bit of time and you need an oven thermometer to make sure that you have hit the correct temperature. Steam sterilization like in an autoclave is typically preferred in practice unless the instruments would be corroded by steam.


--------------------
Let's upgrade our security practices and move toward client-side PGP for encrypted PMs.
My Public PGP Key: hxxps://wwww.shroomery.org/forums/showflat.php/Number/24002249#24002249


Post Extras: Print Post  Remind Me! Notify Moderator
InvisibleDoc_T
Random Dude
Male User Gallery


Folding@home Statistics
Registered: 03/06/09
Posts: 42,395
Loc: Colorado Flag
Re: Oven sterilizing tools [Re: naum]
    #11379950 - 11/03/09 10:40 PM (7 years, 11 months ago)

Quote:

naum said:
but it takes quite a bit of time and you need an oven thermometer to make sure that you have hit the correct temperature.




Nope. Sterilizing metal tools isn't like sterilizing bulk substrate.
Preheat oven to 325. Wrap tools in foil, Place on cookie sheet in a single layer. Bake 30 mins or until golden brown. :awesome:


--------------------
You make it all possible. Doesn't it feel good?


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineRogerRabbitM
Bans for Pleasure
Male User Gallery


Registered: 03/26/03
Posts: 42,018
Loc: USA Mountain Northwest
Last seen: 17 days, 14 hours
Trusted Cultivator
Re: Oven sterilizing tools [Re: Doc_T]
    #11380331 - 11/03/09 11:46 PM (7 years, 11 months ago)

You don't need to pre-sterilize scalpels, inoculating loops, etc.  Wipe with alcohol, and then anything that touches sterile media gets flame sterilized immediately before the transfer or other work.
RR


--------------------
Download Let's Grow Mushrooms



semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
Thomas Edison


Post Extras: Print Post  Remind Me! Notify Moderator
InvisiblenaumM
 User Gallery


Registered: 10/10/07
Posts: 3,774
Re: Oven sterilizing tools [Re: Doc_T]
    #11380347 - 11/03/09 11:48 PM (7 years, 11 months ago)

That should have read the correct oven temperature. Most if not all ovens do not heat to the dial temperature and do not heat uniformly. Dry heat sterilization isn't as simple as pre-heating to dial "325 F" and tossing in the wrapped instruments for 30 minutes.

While there are varying recommendations even within the scientific literature, the consensus seems to be that ~120 minutes at 160 C (320 F) is required for sterilization by dry heat. You can find this information on the web or should be able to find it in any thorough microbiological text.

The reason for needed for the long time is illustrated by Figures 1 and 2 in this fairly old but still relevant and widely cited article[PDF-ncbi.nlm.nih.gov] on the topic of dry heat sterilization. It takes a considerable amount of time for the instruments place in the oven to actually reach 160 C. Before anyone says that oven in the article was not pre-heated, please read the caption carefully and notice that it explicitly states that the oven was preheated.

Faster sterilization can be achieved using higher temperatures, but at the expense of instrument edges which are degraded by dry heat. You can use temperatures somewhat less than 160 C, but sterilization takes considerably more time. A oven with a fan (common on the other side of the pond) will be more effective at providing a stable temperature and thus might require less time for sterilization.

Though if 30 minutes at 325 F on your oven works for you, I won't try contest that it doesn't. However, the standard scientific procedure for dry heat sterilization is 120 minutes at 160 C. Even the most relaxed guidelines I am familiar with (the British Pharmacopeia) states no less than an hour at 160 C.

OP: I think that flame sterilization like RR suggested would be the best idea (easiest and fastest) for the sorts of small instruments described in your post. :thumbup: 


Post Extras: Print Post  Remind Me! Notify Moderator
InvisibleDoc_T
Random Dude
Male User Gallery


Folding@home Statistics
Registered: 03/06/09
Posts: 42,395
Loc: Colorado Flag
Re: Oven sterilizing tools [Re: naum]
    #11381817 - 11/04/09 07:55 AM (7 years, 11 months ago)

I didn't realize there were standards for dry heat  sterilization, I learned something new!

I have a hard time believing that 120 min @ 160C is required, that seems incredible- but I'm not doubting your source or you. Just scratching my head.


--------------------
You make it all possible. Doesn't it feel good?


Post Extras: Print Post  Remind Me! Notify Moderator
Jump to top. Pages: 1

Amazon Shop: Agar, Autoclave, Pressure Cooker

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

Similar ThreadsPosterViewsRepliesLast post
* Oven sterilization of PF jars *DELETED*
( 1 2 all )
Lakefingers 2,749 33 09/17/05 12:18 AM
by HippieChick
* Temperature & Oven Sterilization Terrarium_Guy 897 2 06/23/01 09:26 AM
by Terrarium_Guy
* Oven sterilization....any success stories?
( 1 2 all )
libertaire 2,781 27 10/27/09 10:50 AM
by Doc_T
* oven sterilization godot 932 6 03/10/04 06:16 PM
by mg420
* Oven sterilization luckyjew 1,729 7 01/13/02 01:10 PM
by fungichick
* Oven "Sterilization" Method for PF Jars Terillius 3,288 4 08/18/06 11:22 PM
by Wronguy
* Oven Sterilization Method Success
( 1 2 3 all )
Terillius 5,146 42 08/18/06 09:24 PM
by Wronguy
* oven sterilization of grains. backintheriver 1,524 12 01/19/09 04:02 PM
by German Kahuna

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Roadkill, Magash, Shroomism, george castanza, RogerRabbit, Citric, total, FooMan, 13shrooms, stonesun, cronicr, PussyFart, Tmethyl, Pastywhyte, bodhisatta
643 topic views. 32 members, 101 guests and 28 web crawlers are browsing this forum.
[ Toggle Favorite | Print Topic | Stats ]
Search this thread:

Please support our sponsors.

Copyright 1997-2017 Mind Media. Some rights reserved.

Generated in 0.03 seconds spending 0.005 seconds on 19 queries.