glove box procedure - Mushroom Cultivation

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wykydtron
Stranger

Registered: 11/01/09
Posts: 9
Last seen: 2 years, 8 months

glove box procedure

#11372925 - 11/02/09 09:28 PM (14 years, 5 months ago)

I was looking at a glove box tek on this site, and the instructions said to cut a square in the box and cover it with tyvek. What's the point of this square? The only thing I can think of is that it's for sterilizing the needle so you don't have to keep taking it out of the box. If this is the case do you keep the needle inside the box, poke it through, and heat from the outside? Or, do you keep the needle outside and poke through to the inside to inoculate?

On a related note, when you open the lid to the glove box to put the jars in, after closing the lid, do you spray oust with the jars inside?

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Slimz
.-~*´`*·~-experience-~*´`*·~-.

Registered: 10/03/07
Posts: 3,588
Loc: Maryland

Re: glove box procedure

[Re: wykydtron]
#11372939 - 11/02/09 09:30 PM (14 years, 5 months ago)

circles are fine..

sterilize needle and place in the glovebox as it cools..

after that there is no need to sterilize between jars, just wipe with alcohol swab

--------------------
Lazy Drywall Tek (no powdery mess)

This series will blow your mind and confirm what you already know to be true.
The Pharmacratic Inquisition
Best Thread Ever ! ! !

me if you have questions about lasers

Although i may advise others in a general way regarding all types of mushroom grows, and may even post question from other forums about growing "active" mushrooms, i only grow non-"active" mushrooms and edibles.

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Othyem

Registered: 04/17/09
Posts: 1,935
Last seen: 3 years, 6 months

Re: glove box procedure

[Re: Slimz]
#11372957 - 11/02/09 09:32 PM (14 years, 5 months ago)

Always flame between jars.

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Slimz
.-~*´`*·~-experience-~*´`*·~-.

Registered: 10/03/07
Posts: 3,588
Loc: Maryland

Re: glove box procedure

[Re: Othyem]
#11372973 - 11/02/09 09:34 PM (14 years, 5 months ago)

Quote:

Othyem said:
Always flame between jars.

no need in a GB..

--------------------
Lazy Drywall Tek (no powdery mess)

This series will blow your mind and confirm what you already know to be true.
The Pharmacratic Inquisition
Best Thread Ever ! ! !

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wykydtron
Stranger

Registered: 11/01/09
Posts: 9
Last seen: 2 years, 8 months

Re: glove box procedure

[Re: Slimz]
#11373269 - 11/02/09 10:11 PM (14 years, 5 months ago)

Well, regardless oh whether or not to flame between jars, is that the point of the tyvek square?

It's this one:

http://www.shroomery.org/10524/Glovebox-Construction-and-Design

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Slimz
.-~*´`*·~-experience-~*´`*·~-.

Registered: 10/03/07
Posts: 3,588
Loc: Maryland

Re: glove box procedure

[Re: wykydtron]
#11373302 - 11/02/09 10:14 PM (14 years, 5 months ago)

no clue what that square is for..

--------------------
Lazy Drywall Tek (no powdery mess)

This series will blow your mind and confirm what you already know to be true.
The Pharmacratic Inquisition
Best Thread Ever ! ! !

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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation

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