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OfflineBreakfast Crew
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How important is packing your substrate?
    #11348404 - 10/29/09 09:08 PM (14 years, 4 months ago)

This is one of my test jars using semi-fine brown rice.

The front.


The back.


I am no expert in identifying contamination but clearly something is wrong.  The yellowing seems to be rot (Rice turns yellow when it gets old, go figure) and tends happens where the substrate is more "fluffy". I have made another jar with whole grain packed fairly tight with NO yellowing. Another jar with whole rice packed as loose as stripper that's doing poorly.

Edited by Breakfast Crew (10/29/09 09:13 PM)

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OfflineDamion5050
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Re: How important is packing your substrate? [Re: Breakfast Crew]
    #11348410 - 10/29/09 09:09 PM (14 years, 4 months ago)

Where is your vermiculite ?

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OfflineBreakfast Crew
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Re: How important is packing your substrate? [Re: Damion5050]
    #11348428 - 10/29/09 09:11 PM (14 years, 4 months ago)

Ha now that is a long story to explain.  These are just experiments because I don't trust all the myths on what you need/don't need.

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OfflineGastronomicus
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Re: How important is packing your substrate? [Re: Damion5050]
    #11348429 - 10/29/09 09:11 PM (14 years, 4 months ago)

Quote:

Damion5050 said:
Where is your vermiculite ?




This, also why are your jars half empty?


--------------------
Make my Funk the P Funk, I wants to get Funked up

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InvisibleCH HELL
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Re: How important is packing your substrate? [Re: Damion5050]
    #11348435 - 10/29/09 09:12 PM (14 years, 4 months ago)

Quote:

Breakfast Crew said:
clearly something is wrong..



Quote:

Damion5050 said:
Where is your vermiculite ?


:wink:

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InvisiblePolk_Audio3
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Re: How important is packing your substrate? [Re: Gastronomicus]
    #11348462 - 10/29/09 09:13 PM (14 years, 4 months ago)

You should have just went with the regular tek but, I guess experimenting is awesome.


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OfflineBreakfast Crew
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Re: How important is packing your substrate? [Re: CH HELL]
    #11348472 - 10/29/09 09:14 PM (14 years, 4 months ago)

You say half empty, I say half full. It sure is hard to stay on topic isn't it?

*First post edited, can't believe I had to bold TEST.

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OfflineGastronomicus
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Re: How important is packing your substrate? [Re: Breakfast Crew]
    #11348540 - 10/29/09 09:22 PM (14 years, 4 months ago)

Quote:

Breakfast Crew said:
You say half empty, I say half full.




Half full of contams.


Anyway, this is on topic, you asked "why is my experimental jar not doing well." Comments were made that basically said "why are you not using time tested ingredients?"


--------------------
Make my Funk the P Funk, I wants to get Funked up

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InvisibleDoc_T
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Re: How important is packing your substrate? [Re: Breakfast Crew]
    #11348590 - 10/29/09 09:27 PM (14 years, 4 months ago)

Quote:

Breakfast Crew said:
Ha now that is a long story to explain.  These are just experiments because I don't trust all the myths on what you need/don't need.




Verm isn't a myth.
Another non-myth: rice can be used, but it's sticky. Use rye or wbs instead.

So, do I want to know what kind of lids you are using?


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You make it all possible. Doesn't it feel good?

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OfflineBreakfast Crew
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Re: How important is packing your substrate? [Re: Doc_T]
    #11348721 - 10/29/09 09:41 PM (14 years, 4 months ago)

Experimentation are fun. Here is me being silly trying to get an isolate from these cute mushrooms.


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OfflineBreakfast Crew
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Re: How important is packing your substrate? [Re: Breakfast Crew]
    #11363048 - 11/01/09 02:27 PM (14 years, 4 months ago)

Same jar 3 days later.



The mycelium is eating that yellow stuff, maybe I was wrong?

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OfflineLucienis
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Re: How important is packing your substrate? [Re: Breakfast Crew]
    #11363218 - 11/01/09 02:52 PM (14 years, 4 months ago)

It will probably eat it, but it might stall or take absolutely forever to colonize...

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Invisibleambargh
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Re: How important is packing your substrate? [Re: Lucienis]
    #11364007 - 11/01/09 05:06 PM (14 years, 4 months ago)

you should take the foil off so it can breathe...


--------------------
"The fact that we live at the bottom of a deep gravity well, on the surface of a gas covered planet going around a nuclear fireball 90 million miles away and think this to be normal is obviously some indication of how skewed our perspective tends to be.." - Douglas Adams

ambargh's easy agar

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InvisibleDoc_T
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Re: How important is packing your substrate? [Re: ambargh]
    #11364028 - 11/01/09 05:09 PM (14 years, 4 months ago)

If there is a filter in the lid.


--------------------
You make it all possible. Doesn't it feel good?

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Offlinerottypup
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Re: How important is packing your substrate? [Re: Lucienis]
    #11364528 - 11/01/09 06:40 PM (14 years, 4 months ago)

Quote:

Lucienis said:
It will probably eat it, but it might stall or take absolutely forever to colonize...




what a jerk

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Invisibleprismism
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Re: How important is packing your substrate? [Re: rottypup]
    #11364723 - 11/01/09 07:13 PM (14 years, 4 months ago)

Quote:

rottypup said:
Quote:

Lucienis said:
It will probably eat it, but it might stall or take absolutely forever to colonize...




what a jerk




lol. what?


--------------------
ephemeral anomalous

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OfflineBreakfast Crew
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Re: How important is packing your substrate? [Re: prismism]
    #11366163 - 11/01/09 10:44 PM (14 years, 4 months ago)

Two holes, one caulked and one with poly. The foil isn't on tight and is actually very loose. I'm going to leave it there because it doesn't seem to be doing any harm.

On a side note I've just started doing some more agar work and this damn condensation is ticking me off. Any tips?

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OfflineLancaster
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Re: How important is packing your substrate? [Re: prismism]
    #11366233 - 11/01/09 10:59 PM (14 years, 4 months ago)

Quote:

prismism said:
Quote:

rottypup said:
Quote:

Lucienis said:
It will probably eat it, but it might stall or take absolutely forever to colonize...




what a jerk




lol. what?




--------------------

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Invisible13shroomsM
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Re: How important is packing your substrate? [Re: Lancaster]
    #11366328 - 11/01/09 11:19 PM (14 years, 4 months ago)

Quote:

this damn condensation is ticking me off. Any tips?




dont pour so hot. :sun: 
stack your dishes on top of one another with a empty on top so the rising heat and condensation collects in the top empty dish instead of each individual one. :super:


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OfflineMontock
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Re: How important is packing your substrate? [Re: 13shrooms]
    #11366361 - 11/01/09 11:28 PM (14 years, 4 months ago)

i looks like you didnt use any vermiculite, in no way shape or form am i an expert with the pf tek, i went basicly straight to grains, but i belive the idea is to properly wet the vermicutelite and than have what ever your substrate is coat the wet particls of verm as completely as possible, its possible your moisture content is fucked up, and what little experince i do have with the pf tek, having a tightly packed jar takes forever, but if you have it to loose, the myc obviously cant make the jump., with my massive failures in the past with the pf tek the main culprets were 1. incubator frying the jars, 2. in proper sterilization, 3 fucked up water content


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