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Amazon Shop: ½ Pint Jars, Laminar Flow Hood

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OfflineTaterCaboose
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Registered: 11/01/09
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Oven instead of glove box
    #11363727 - 11/01/09 06:23 PM (7 years, 11 months ago)

How would you prepare the oven for sterility? Oven cleaner or just heating it up for a little while and letting it cool?
It seems like using an oven would would be slightly difficult (especially since mine doesn't have a light.


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OfflineThyrax
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Re: Oven instead of glove box [Re: TaterCaboose]
    #11363737 - 11/01/09 06:24 PM (7 years, 11 months ago)

yo oven tek is bullshit, get a glove box dude.


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OfflineTaterCaboose
Somethings Fucky...


Registered: 11/01/09
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Re: Oven instead of glove box [Re: Thyrax]
    #11363750 - 11/01/09 06:26 PM (7 years, 11 months ago)

I was thinking the exact same thing. It almost seems like you would be more prone to risk contam in the oven than in open room...


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Offlinesecondhand
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Re: Oven instead of glove box [Re: TaterCaboose]
    #11363766 - 11/01/09 06:29 PM (7 years, 11 months ago)

The second you open the oven doors, you can almost guarantee that possible contamination will enter your oven.


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OfflineThyrax
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Re: Oven instead of glove box [Re: secondhand]
    #11363786 - 11/01/09 06:33 PM (7 years, 11 months ago)

Glove box is so cheap and easy to do than there is no reason to not have one.


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OfflineTaterCaboose
Somethings Fucky...


Registered: 11/01/09
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Re: Oven instead of glove box [Re: Thyrax]
    #11363827 - 11/01/09 06:39 PM (7 years, 11 months ago)

I just read an older post about a guy using an upsidedown table with a sheet over the legs (like a little fort kids play in) and spraying it down with lysol on the inside, sprayed his clothes, gloves, breathing mask, hair, everything, and just works inside that. Much easier than an oven, and maybe not as technical as a glovebox but pretty interesting nonetheless.


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OfflineThyrax
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Re: Oven instead of glove box [Re: TaterCaboose]
    #11363847 - 11/01/09 06:42 PM (7 years, 11 months ago)

glove box = 10$ and 10 minute to do. Why wouldn't you use it ?


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OfflineTaterCaboose
Somethings Fucky...


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Re: Oven instead of glove box [Re: Thyrax]
    #11363883 - 11/01/09 06:46 PM (7 years, 11 months ago)

I will indeed.


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Invisibleambargh
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Re: Oven instead of glove box [Re: TaterCaboose]
    #11363918 - 11/01/09 06:51 PM (7 years, 11 months ago)

Think about all that black shit on the bottom of your oven.


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"The fact that we live at the bottom of a deep gravity well, on the surface of a gas covered planet going around a nuclear fireball 90 million miles away and think this to be normal is obviously some indication of how skewed our perspective tends to be.." - Douglas Adams

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OfflineThyrax
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Re: Oven instead of glove box [Re: TaterCaboose]
    #11363919 - 11/01/09 06:52 PM (7 years, 11 months ago)

:thumbup:


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Invisibleambargh
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Re: Oven instead of glove box [Re: Thyrax]
    #11363924 - 11/01/09 06:53 PM (7 years, 11 months ago)

lol - that would be the last place I would try and set up a sterile field.


--------------------
"The fact that we live at the bottom of a deep gravity well, on the surface of a gas covered planet going around a nuclear fireball 90 million miles away and think this to be normal is obviously some indication of how skewed our perspective tends to be.." - Douglas Adams

ambargh's easy agar


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OfflineTaterCaboose
Somethings Fucky...


Registered: 11/01/09
Posts: 272
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Re: Oven instead of glove box [Re: ambargh]
    #11363944 - 11/01/09 06:56 PM (7 years, 11 months ago)

Definantly. Isnt it odd how we eat out of it though? :crazy2:


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Invisibleambargh
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Re: Oven instead of glove box [Re: TaterCaboose]
    #11363950 - 11/01/09 06:58 PM (7 years, 11 months ago)

I'll eat out of anything.


--------------------
"The fact that we live at the bottom of a deep gravity well, on the surface of a gas covered planet going around a nuclear fireball 90 million miles away and think this to be normal is obviously some indication of how skewed our perspective tends to be.." - Douglas Adams

ambargh's easy agar


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OfflineBDiddy
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Registered: 09/04/09
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Re: Oven instead of glove box [Re: ambargh]
    #11364129 - 11/01/09 07:28 PM (7 years, 11 months ago)

I just made a still air box out of a cardboard box with all the seams taped up holes cut for my arms and plastic wrap taped over the top. Has worked perfectly so far!


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Offlinefig
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Re: Oven instead of glove box [Re: BDiddy]
    #11515168 - 11/24/09 12:05 AM (7 years, 10 months ago)

i have used the oven tek with success before
but it could have been dumb luck.

the reason it works is the heat pushes the mold, dust,ect up and away from the inside cause heat rises, but u have to be careful not to burn the fuck out of your self.

I cant seem to find the tek so here is the short version.

you heat up the oven after cleaning it
and let it cure for 30 min or so to kill anything inside
then open the door and and leave the heat on medium or low
now u want to work on a rack so pull one  abt mid way up in the oven out half way so it can cool a little but u have to work quick cause your jars and everything  will heat up do your g2g or what ever your doing.
but it duz work just outdated (not recommended) now i think. i used it years ago.

i recommend using a shmuv box, flow hood ,or glove box all pretty easy to make with little money.


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OfflineBreakfast Crew
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Registered: 01/20/09
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Re: Oven instead of glove box [Re: fig]
    #11515201 - 11/24/09 12:10 AM (7 years, 10 months ago)

oh so it pushes dirty air up into your work space, awesome.


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OfflineBrennus
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Re: Oven instead of glove box [Re: fig]
    #11515206 - 11/24/09 12:11 AM (7 years, 10 months ago)

Quote:

fig said:
i have used the oven tek with success before
but it could have been dumb luck.

the reason it works is the heat pushes the mold, dust,ect up and away from the inside cause heat rises, but u have to be careful not to burn the fuck out of your self.

I cant seem to find the tek so here is the short version.

you heat up the oven after cleaning it
and let it cure for 30 min or so to kill anything inside
then open the door and and leave the heat on medium or low
now u want to work on a rack so pull one  abt mid way up in the oven out half way so it can cool a little but u have to work quick cause your jars and everything  will heat up do your g2g or what ever your doing.
but it duz work just outdated (not recommended) now i think. i used it years ago.

i recommend using a shmuv box, flow hood ,or glove box all pretty easy to make with little money.




Moving air, unless in the form of laminar flow, while doing sterile work will pretty much doom you to failure if you have any kind of spore load in your house.

Some idiot posted the oven tek years back because he got lucky and it 'worked'. It doesn't.


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Amazon Shop: ½ Pint Jars, Laminar Flow Hood

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