Home | Community | Message Board


Avalon Magic Plants
Please support our sponsors.

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Amazon Shop: ½ Pint Jars, 10cc Syringe

Jump to first unread post. Pages: 1
OfflineMycoRunner
Stranger


Registered: 09/23/09
Posts: 135
Last seen: 1 month, 26 days
Teks on Sterilizing Syringes
    #11363227 - 11/01/09 04:53 PM (8 years, 20 days ago)

I've heard of so many ways to sterilize syringes, but no one seems to agree on a method. I don't really want to throw them in the PC because I don't really know what the best way is. Do I take apart the syringe? Do I fill it with water?

What is your method? Is there a way of simply sucking up boiling water? I tried sucking up iso alcohol...

Thanks for any input


--------------------
:nyan:


Post Extras: Print Post  Remind Me! Notify Moderator
InvisiblePsYLoW
Hyperspace

Registered: 10/08/09
Posts: 132
Loc: Middle of Nowhere
Re: Teks on Sterilizing Syringes [Re: MycoRunner]
    #11363266 - 11/01/09 04:59 PM (8 years, 20 days ago)



--------------------
:jah::leaf::leaf::leaf: I see marijuana trees growing wild and free! :leaf::leaf::leaf::jah:


Post Extras: Print Post  Remind Me! Notify Moderator
InvisibleShea25
Just some guy
Male


Registered: 01/27/09
Posts: 7,772
Loc: Westcoast Canada Flag
Trusted Cultivator
Re: Teks on Sterilizing Syringes [Re: MycoRunner]
    #11363269 - 11/01/09 04:59 PM (8 years, 20 days ago)

Boil water for a while. then suck it back in the syringe wait 1 min and expel do that 4-5 times. then on the last one keep the water in it cap the syringe and let cool down at room temp


Post Extras: Print Post  Remind Me! Notify Moderator
InvisibleDoc_T
Random Dude
Male User Gallery


Folding@home Statistics
Registered: 03/06/09
Posts: 42,395
Loc: Colorado Flag
Re: Teks on Sterilizing Syringes [Re: Shea25]
    #11363300 - 11/01/09 05:05 PM (8 years, 20 days ago)

I PC them in foil. Put a cap on, and it stays sterile inside until you use it.


--------------------
You make it all possible. Doesn't it feel good?


Post Extras: Print Post  Remind Me! Notify Moderator
Invisiblemyceleus_rex
seeker
Male User Gallery


Registered: 07/01/09
Posts: 1,581
Loc: alized
Re: Teks on Sterilizing Syringes [Re: Doc_T]
    #11363327 - 11/01/09 05:09 PM (8 years, 20 days ago)

Quote:

Doc_T said:
I PC them in foil. Put a cap on, and it stays sterile inside until you use it.



half hour at 15 psi? I've been doing the boiling water routine, but the PC sounds better, especially for several at a time.


--------------------
Cervantes' sclerotia thread    Citric's self-healing lid tek  Agar's Grain LC  Breaking Up Spawn Jars

World's Ugliest Cat?

If ignorance is bliss, why aren't there more happy people?


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineMycoRunner
Stranger


Registered: 09/23/09
Posts: 135
Last seen: 1 month, 26 days
Re: Teks on Sterilizing Syringes [Re: myceleus_rex]
    #11363427 - 11/01/09 05:27 PM (8 years, 20 days ago)

HEy thanks for the replies. I think next time I'm going to try the boiling water tek.


--------------------
:nyan:


Post Extras: Print Post  Remind Me! Notify Moderator
InvisibleDoc_T
Random Dude
Male User Gallery


Folding@home Statistics
Registered: 03/06/09
Posts: 42,395
Loc: Colorado Flag
Re: Teks on Sterilizing Syringes [Re: myceleus_rex]
    #11363446 - 11/01/09 05:31 PM (8 years, 20 days ago)

Quote:

myceleus_rex said:
Quote:

Doc_T said:
I PC them in foil. Put a cap on, and it stays sterile inside until you use it.



half hour at 15 psi? I've been doing the boiling water routine, but the PC sounds better, especially for several at a time.




At least 30, I just put syringes or needles or caps or whatever in with whatever batch of jars I'm cooking.
If it's quarts of grain, they'll get 120@15 psi. Never had any of these things melt, but it's all brand name medical-grade stuff.
YMMV, user assumes all risks, void where prohibited, etc.


--------------------
You make it all possible. Doesn't it feel good?


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineSpongiform
Some Cow
Male User Gallery Arcade Champion: Cat Bowling

Registered: 08/22/07
Posts: 3,994
Loc: Greener Pastures
Last seen: 2 years, 2 months
Trusted Cultivator
Re: Teks on Sterilizing Syringes [Re: Doc_T]
    #11363479 - 11/01/09 05:36 PM (8 years, 20 days ago)

I rinse with hot water to remove any sediment/gunk.  Wrap in tinfoil and PC along with grains.


--------------------
Spongiform's Plastic Tek - An Alternative to Glass
Spongi's PF Block Tek & Automated Shotgun Project.


Post Extras: Print Post  Remind Me! Notify Moderator
Offlinescifipirate
Jug Fuckler
Male User Gallery


Registered: 01/17/09
Posts: 759
Loc: FL Flag
Last seen: 1 year, 6 months
Re: Teks on Sterilizing Syringes [Re: Spongiform]
    #11363497 - 11/01/09 05:39 PM (8 years, 20 days ago)

I put 10cc of water and some air in the syringe, wrap it in foil and PC it with my jars


Post Extras: Print Post  Remind Me! Notify Moderator
Invisiblemyceleus_rex
seeker
Male User Gallery


Registered: 07/01/09
Posts: 1,581
Loc: alized
Re: Teks on Sterilizing Syringes [Re: Spongiform]
    #11363514 - 11/01/09 05:41 PM (8 years, 20 days ago)

Hmmm. So in that case they get a full 90 minutes. I would have been concerned about them  warping, if not melting. I'd think 20-30 min would work fine otherwise.


--------------------
Cervantes' sclerotia thread    Citric's self-healing lid tek  Agar's Grain LC  Breaking Up Spawn Jars

World's Ugliest Cat?

If ignorance is bliss, why aren't there more happy people?


Post Extras: Print Post  Remind Me! Notify Moderator
InvisibleDoc_T
Random Dude
Male User Gallery


Folding@home Statistics
Registered: 03/06/09
Posts: 42,395
Loc: Colorado Flag
Re: Teks on Sterilizing Syringes [Re: myceleus_rex]
    #11363543 - 11/01/09 05:46 PM (8 years, 20 days ago)

Quote:

Spongiform said:
I rinse with hot water to remove any sediment/gunk.




Good advice.

Quote:

myceleus_rex said:
I'd think 20-30 min would work fine otherwise.



Sure, if it's empty and the plunger is all the way in, it's all surface. You could sterilize it in like 15 minutes, probably.
But I just keep a few extras on hand, and then whatever is dirty goes into that day's PC run.


--------------------
You make it all possible. Doesn't it feel good?


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineSpongiform
Some Cow
Male User Gallery Arcade Champion: Cat Bowling

Registered: 08/22/07
Posts: 3,994
Loc: Greener Pastures
Last seen: 2 years, 2 months
Trusted Cultivator
Re: Teks on Sterilizing Syringes [Re: Doc_T]
    #11363576 - 11/01/09 05:50 PM (8 years, 20 days ago)

I've been re-using the same syringes for about a year now, PC'd 5 or 6 times each at least and no visible/apparent damage yet.


--------------------
Spongiform's Plastic Tek - An Alternative to Glass
Spongi's PF Block Tek & Automated Shotgun Project.


Post Extras: Print Post  Remind Me! Notify Moderator
Offlinescifipirate
Jug Fuckler
Male User Gallery


Registered: 01/17/09
Posts: 759
Loc: FL Flag
Last seen: 1 year, 6 months
Re: Teks on Sterilizing Syringes [Re: Spongiform]
    #11364505 - 11/01/09 08:37 PM (8 years, 20 days ago)

yeah, syringes ain't scared of some PCing


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineMnkyLuvr
da mghty bsh
Male


Registered: 08/05/09
Posts: 44
Last seen: 2 years, 5 months
Re: Teks on Sterilizing Syringes [Re: scifipirate]
    #11378420 - 11/03/09 06:54 PM (8 years, 18 days ago)

hmmm.  I've been PC'ing my big ones (60ML) wrapped in foil and I get slight warping.  The plungers are a little leaky and the needles don't go in totally straight. 

I've had better luck with boiling water...haven't had a contam yet...but never say never off course.


--------------------
______________________________________________________
dem stones, dem stones, dem happy stones
Psilocybe mexicana & P. tampanensis (sclerotia) FAQ

Mexicana A La Quixote ||  Easier Than Cubes!!!


Post Extras: Print Post  Remind Me! Notify Moderator
Invisibledaytripper05
Psychonaut
Male User Gallery


Folding@home Statistics
Registered: 10/31/06
Posts: 6,962
Loc: In my garden
Re: Teks on Sterilizing Syringes [Re: MycoRunner]
    #11378533 - 11/03/09 07:09 PM (8 years, 18 days ago)

If you want to have sterile water inside the syringe, I would go with the boiling water method. But for empty sterile syringes I use my PC.


Post Extras: Print Post  Remind Me! Notify Moderator
Offlinelosfreddy
Composter
Male User Gallery


Registered: 02/16/04
Posts: 8,639
Last seen: 1 hour, 58 minutes
Re: Teks on Sterilizing Syringes [Re: MnkyLuvr]
    #11378610 - 11/03/09 07:18 PM (8 years, 18 days ago)

i wouldnt worry too much about having air in it when sterilizing. U usually cant suck up all the spore solution from shot glass anyways.
I boil in a little camping pan thing.  I dont like to use big pots..  I take the needle and caps off and boil it in drinking water at high temp.  Expell and suck in the water in a few times; switch off between two syringes. Dont leave them sitting for too long on a bare metal spot cause they will melt. Use a tweezers or needle nose pliers to grab the needles from water and put back on syringe.  Shake the cap to get access water out or u may get ur spores wet when u go to scrap the print. u can hold it a few cms above water when doing this.  Work in the steam if u have to



Put needles on a papertowel, wrap it and put it in a bag to cool..

Then i think u got it from here. Once cool wipe shot glass, let dry and do your thing.



Heres a picture of my dog!


--------------------
Quit reading my mind!


Edited by losfreddy (11/03/09 09:23 PM)


Post Extras: Print Post  Remind Me! Notify Moderator
Invisiblemyceleus_rex
seeker
Male User Gallery


Registered: 07/01/09
Posts: 1,581
Loc: alized
Re: Teks on Sterilizing Syringes [Re: losfreddy]
    #11378869 - 11/03/09 07:59 PM (8 years, 18 days ago)

Any difference observed between brands of syringes and how they hold up to repeated sterilization, esp. PC?


--------------------
Cervantes' sclerotia thread    Citric's self-healing lid tek  Agar's Grain LC  Breaking Up Spawn Jars

World's Ugliest Cat?

If ignorance is bliss, why aren't there more happy people?


Post Extras: Print Post  Remind Me! Notify Moderator
Offlinelosfreddy
Composter
Male User Gallery


Registered: 02/16/04
Posts: 8,639
Last seen: 1 hour, 58 minutes
Re: Teks on Sterilizing Syringes [Re: myceleus_rex]
    #11379334 - 11/03/09 09:13 PM (8 years, 18 days ago)

Ive used the ones in the pics for a couple years at least, but all of my syringes have been used several times.. Ones in pics i got at feed store. They all have a burnt/ melted spot from stove and one is a little warped; but are all still in use. The ones from sporeworks have big gauge needle and are awesome to use.  Spores get stuck in the nose of after several uses, but i really doesnt make a difference cause it all gets sterilized anyways.


--------------------
Quit reading my mind!


Post Extras: Print Post  Remind Me! Notify Moderator
Offlinelosfreddy
Composter
Male User Gallery


Registered: 02/16/04
Posts: 8,639
Last seen: 1 hour, 58 minutes
Re: Teks on Sterilizing Syringes [Re: losfreddy]
    #11379350 - 11/03/09 09:16 PM (8 years, 18 days ago)

ive never used a PC for syringes, so IDK about that


--------------------
Quit reading my mind!


Post Extras: Print Post  Remind Me! Notify Moderator
Jump to top. Pages: 1

Amazon Shop: ½ Pint Jars, 10cc Syringe

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

Similar ThreadsPosterViewsRepliesLast post
* Success rate at first time making a sterile syring tekramrepus 2,741 9 09/18/02 10:23 PM
by PSILLYSIMON
* pf tek sterilization question theseeeper1 2,203 16 06/04/09 09:39 PM
by RogerRabbit
* Grain Tek Sterilization Zwieback0 1,067 7 10/17/02 06:48 AM
by Snobrdr311
* Re: Shroom Wiz Tek & Sterility Anonymous 1,447 9 12/02/99 11:26 PM
by Anonymous
* Oven Tek or Plastic Bag Tek sterile enough? chrisdab 814 2 05/20/04 09:57 AM
by YesItsMe
* PF-Tek Sterilization ShroomTube 2,156 12 12/19/08 01:44 AM
by J3illy
* casing tek sterility grandesign 269 0 08/19/07 02:54 PM
by grandesign
* PF-Tek Sterilizing Jars ... MustardMan 892 1 05/22/06 07:07 PM
by Atheist

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Roadkill, Magash, Shroomism, george castanza, RogerRabbit, Citric, total, FooMan, 13shrooms, stonesun, cronicr, PussyFart, Tmethyl, Pastywhyte, bodhisatta
719 topic views. 14 members, 60 guests and 18 web crawlers are browsing this forum.
[ Toggle Favorite | Print Topic | Stats ]
Search this thread:
Out-Grow.com - Mushroom Growing Kits & Supplies
Please support our sponsors.

Copyright 1997-2017 Mind Media. Some rights reserved.

Generated in 0.039 seconds spending 0.006 seconds on 19 queries.