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Offlinekoopa_troopa
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Developing psilocybin/psilocin Negative Myc. but Grows Psilo Fruits
    #11360032 - 10/31/09 11:22 PM (7 years, 11 months ago)

I was wondering if anyone has ever attempted to cultivate a Cube strain in which the mycelium contained no actives yet still produced active fruitbodies? I usually research a topic to exhaustive detail before posting but figured this one time I would throw a question out hoping for a thorough response from a trusted pro out there.

If this would indeed be possible it would be a huge development for both spore suppliers and newbs as well as for experienced cultivators since it would allow us to legally exchange isolates and real "strains" that would actually make a difference instead of the current marketing ploy of cube strain names.

I was also wondering on the feasibility of producing mass quantities of mycelium through liquid cultures then filtering out the liquid leaving the mycelial mass which could be dehydrated into a powder. After dehydration I wondered if the actives could be extracted without significantly harming the mycelial powder which could then be sold/trasfered/traded legally and hopefully be reanimated through a simple hydration process (imagine the simplicity of adding a couple scoops of mycelium powder to a hydrated substrate and voila!)

Any input on the technical and theoretical feasibility of this would be greatly appreciated!


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InvisibleKnobby Tops
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Re: Developing psilocybin/psilocin Negative Myc. but Grows Psilo Fruits [Re: koopa_troopa]
    #11360042 - 10/31/09 11:25 PM (7 years, 11 months ago)

I think nature already solved this problem... they are called spores....

maybe slightly harder to cultivate, but not hard and they are totally psychoactive free.


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OfflineLucas89
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Re: Developing psilocybin/psilocin Negative Myc. but Grows Psilo Fruits [Re: koopa_troopa]
    #11360215 - 11/01/09 12:13 AM (7 years, 11 months ago)

hmm this is a good idea, but ignorance would jump all over this, and still ban anything to do with psilocybin mushrooms unfortunately. over here, spores which contain no psilocybin are illegal.:sad:

sucks

but i judge criminality not by the law, but by morals and humanity.


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Offlinekoopa_troopa
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Re: Developing psilocybin/psilocin Negative Myc. but Grows Psilo Fruits [Re: Knobby Tops]
    #11360216 - 11/01/09 12:13 AM (7 years, 11 months ago)

thanks for the response but Im pretty sure I already knew that.. next time try answering the question that was asked, that would actually benefit the poster


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Offlinekoopa_troopa
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Re: Developing psilocybin/psilocin Negative Myc. but Grows Psilo Fruits [Re: koopa_troopa]
    #11364401 - 11/01/09 08:16 PM (7 years, 11 months ago)

I believe you missed the point.. do you seriously think I was unaware of such rudementary knowledge?  The idea behind this is that it would allow noobs and other cultivators the ability to aquire genetically superior strains already isolated for their desirable qualities... this would in theory make it possible for some to create super strains by allowing gifted cultivators to work back and forth on the same isolates improving them in their own ways..

If the basic premise is not possible than this thread is pretty damn useless, just wondering if any of the big wigs had any thoughts upon the idea.


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OfflineBeelzebozo
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Re: Developing psilocybin/psilocin Negative Myc. but Grows Psilo Fruits [Re: Knobby Tops]
    #11367875 - 11/02/09 11:26 AM (7 years, 11 months ago)

Quote:

Knobby Tops said:
I think nature already solved this problem... they are called spores....

maybe slightly harder to cultivate, but not hard and they are totally psychoactive free.




Did you even read his post?


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Offlineblackout
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Re: Developing psilocybin/psilocin Negative Myc. but Grows Psilo Fruits [Re: Beelzebozo]
    #11377168 - 11/03/09 03:48 PM (7 years, 11 months ago)

It would depend on laws, I would guess many already cover this issue, like some laws might make growing cannabis plants illegal, even if it is a hemp strain seedling with no THC.

And remember the trading of isolates was legal in many places in europe yet was surprisingly not that popular. In holland you could get many different strains which were not available as spores from the regular vendors. I never got around to cloning a cube, I did try commercial sclerotia but had no luck. I did buy pure strains from spain many years ago, and several sites do still sell isolates still legal in places.


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Edited by blackout (11/03/09 03:57 PM)


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Offlinekoopa_troopa
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Re: Developing psilocybin/psilocin Negative Myc. but Grows Psilo Fruits [Re: blackout]
    #11386332 - 11/04/09 08:19 PM (7 years, 11 months ago)

found a post by anno in this thread http://www.shroomery.org/forums/showflat.php/Number/2111589/fpart/2/vc/1

From http://diseyes.lycaeum.org/teo/peele.txt

"Take Psilocybe Cyanescens for example. Most of you might know that this is one of the most powerful of all psychoactive mushrooms. I have talked to other people who ate this mushroom. Some, who by the way could boast about how many Psilocybe cybensis mushrooms they could eat, can't
even look at a mushroom they might see in the grocery store..........I would advise anybody to go lightly on this one. The point I want to make is that this mushroom, and Psilocybe pelliculosa, prove the somewhat theory
I have. When these mushrooms are grown under liquid media, they do not produce any psilocybin or psilocin. This was also found to be true by P.Catalfomo and V.E. Tyler, Jr.. They published the same findings about Psilocybe cyanescens and Psilocybe pelliculosa......they do not produce
psilocybing or any other analogs in liquid media
(Catalfomo, P. and V.E. Tyler, Jr. _The production of psilocybin in submerged culture of Psilocybe cubensis_ LLoydia 27:53-63, 1964). However, once the mycelium of one of these two is transferred to an agar or grain media, it does produce psilocybin and psilocin."



Pretty interesting.. so theoretically it IS legal to trade LCs of P Cyans and a few other psilocybe species :smile:


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OfflineAlan RockefellerM
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Re: Developing psilocybin/psilocin Negative Myc. but Grows Psilo Fruits [Re: koopa_troopa]
    #11390133 - 11/05/09 12:52 PM (7 years, 11 months ago)

Quote:

Pretty interesting.. so theoretically it IS legal to trade LCs of P Cyans and a few other psilocybe species :smile:





Yea.

Also remember that testing for psilocybin is an involved process, there is no easy field test they can do on a mushroom or agar dish to see if it has psilocybin.  They have to send it to the crime lab and run it through GCMS, a few months later they get their result. 

So if they find a petri dish or LC in the mail, its very unlikely that they will get it tested and then get you in trouble based on the results of the test.  Especially if they are not packaged like drugs.


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Invisiblebadman
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Re: Developing psilocybin/psilocin Negative Myc. but Grows Psilo Fruits [Re: Alan Rockefeller]
    #11392603 - 11/05/09 07:42 PM (7 years, 11 months ago)

But its still illegal to send cultures, right??


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Offlinekoopa_troopa
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Re: Developing psilocybin/psilocin Negative Myc. but Grows Psilo Fruits [Re: badman]
    #11400994 - 11/06/09 11:12 PM (7 years, 11 months ago)

well... technically its only illegal if the cultures contain psilocybes..


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Invisiblebadman
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Re: Developing psilocybin/psilocin Negative Myc. but Grows Psilo Fruits [Re: koopa_troopa]
    #11402730 - 11/07/09 07:41 AM (7 years, 11 months ago)

But us at home cant determine whether they do or dont so we assume they do contain actives and not send them. Like blaackout said it used to be legal in europe... im gonna dig out some UK laws on dugs.


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Invisiblefastfred
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Re: Developing psilocybin/psilocin Negative Myc. but Grows Psilo Fruits [Re: badman]
    #11473548 - 11/17/09 02:36 PM (7 years, 11 months ago)

I worked on an active-free cube for awhile and might pick up on it again.  It really shouldn't be too hard to do.

As far as extracting from myc...  There is little to no actives in myc depending on the stage of growth.  Basically it's not worth it.


-FF


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OfflineLennybernadino
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Re: Developing psilocybin/psilocin Negative Myc. but Grows Psilo Fruits [Re: fastfred]
    #11478795 - 11/18/09 04:03 PM (7 years, 11 months ago)

My Panaelous Cyanesens formed sclorotia wich surpriesed me I did not know Pancyans did this, and i ate one, not bitter but actrually sweet almondy flavor like the Agaricus arvensis group,i am pretty sure it did not produce psilocybin and the sceloroti does not stain blue either! Anyway here is and idea that does not really help me but why doesn´t somone out there one of the bigwigs like RR start working on the developement of cold weather strains of active dung loving mushrooms? then they can be spread all over the place naturally .


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OfflineRogerRabbitM
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Re: Developing psilocybin/psilocin Negative Myc. but Grows Psilo Fruits [Re: Lennybernadino]
    #11478861 - 11/18/09 04:15 PM (7 years, 11 months ago)

Quote:

Anyway here is and idea that does not really help me but why doesn´t somone out there one of the bigwigs like RR start working on the developement of cold weather strains of active dung loving mushrooms? then they can be spread all over the place naturally .




Because it's not generally cold weather that inhibits species like cubensis from spreading.  Seattle is milder in climate than east texas, yet cubes thrive in the latter, but not the former.  It has as much or more to do with rainfall patterns.  In the southern areas that cubes are native to, they have thunderstorms all summer, which keep the mycelium in dung piles from drying completely out.  In the Pacific Northwest, it often doesn't rain at all from late May until September/October and the ground gets tender box dry.  Mycelium on a surface cow pie would dry totally out and die.

The bottom line is different species evolved in different climates and most won't survive in the wild outside their native conditions.
RR


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OfflineLennybernadino
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Re: Developing psilocybin/psilocin Negative Myc. but Grows Psilo Fruits [Re: RogerRabbit]
    #11483801 - 11/19/09 08:10 AM (7 years, 10 months ago)

WEll that hole scelorotia forming thing seems to be a way for surface dung inhabiting mushrooms to survive dry spells, maybe one of those could be adapted to cold weather or Pan. sub. They are adapted to the North West weather patterns and they often like dung .


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Invisiblenooneman
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Re: Developing psilocybin/psilocin Negative Myc. but Grows Psilo Fruits [Re: Lennybernadino]
    #11487307 - 11/19/09 07:36 PM (7 years, 10 months ago)

Quote:

Lennybernadino said:
WEll that hole scelorotia forming thing seems to be a way for surface dung inhabiting mushrooms to survive dry spells, maybe one of those could be adapted to cold weather or Pan. sub. They are adapted to the North West weather patterns and they often like dung.



Aren't there already psychoactive species that grow in the northwest natively? Besides, what's the point of spreading cubensis more than it already is, anyway? Psychoactive mushrooms are already pretty well spread out.. :shrug: Seems pretty useless to me. Cubes are nice, but so are a lot of other mushrooms. Cubes are already so spread out that RR has even been contaminated by cubensis before.

In the summer, I bet everyone will be asking for a heat resistant strain. :tongue:


Edited by nooneman (11/19/09 07:36 PM)


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OfflineLennybernadino
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Re: Developing psilocybin/psilocin Negative Myc. but Grows Psilo Fruits [Re: nooneman]
    #11492515 - 11/20/09 02:40 PM (7 years, 10 months ago)

They are not necessarily spread out in every neighborhood, or region, I have lived in places where I mushrom hunted a lot and only found active mushroom once Ps. Cyanescens growing in somones secret outdoor patch (yes I have been occasionally known to intrude back yards to look for mushrooms in my youth) I left them because they were placesd there on purpose I do not steal . I should have contactd tehm and sked themm to share some mycelium with me but I was not into growing those days .
  jEr


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