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Wimy
weiliiinmyyard
Registered: 08/25/09
Posts: 5,659
Loc: SE USA
Last seen: 3 years, 10 months
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Lids.
#11351050 - 10/30/09 10:41 AM (14 years, 4 months ago) |
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Band-aids are pretty sterile I think so wouldnt it be alright to just put a small square of band-aid over the inoc hole after pulling the cooled down jar out of the pc, and then replacing it with another one in the glovebox after inoculation? The polyfil filters seem to work great if you don't stick a huge horse needle through them. lol.
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myco_mikey_mo
Mycologist For Hire
Registered: 09/11/09
Posts: 1,460
Loc: The PNW
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Re: Lids. [Re: Wimy]
#11351059 - 10/30/09 10:43 AM (14 years, 4 months ago) |
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yeah that works well. i use a small piece of tyvek to cover the hole and then cover that with the sticky part of a band aid
-------------------- -Give a man some mushies, He'll trip for a day...Teach a man to cultivate, He'll trip for a lifetime- Self healing Tyvek and Silcone lids for Trade... PM me Looking for anybody with extra spawn bags for trade... PM me
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fig
spore collector
Registered: 10/01/01
Posts: 724
Loc: usa east TN blue ridge mt...
Last seen: 2 years, 5 months
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band aids also absorb moisture dont know if this is a good thing or not. i used them in my goggles when snowboarding to keep them from fogging up lol
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Wimy
weiliiinmyyard
Registered: 08/25/09
Posts: 5,659
Loc: SE USA
Last seen: 3 years, 10 months
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Re: Lids. [Re: fig]
#11351177 - 10/30/09 11:18 AM (14 years, 4 months ago) |
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Quote:
myco_mikey_mo said: yeah that works well. i use a small piece of tyvek to cover the hole and then cover that with the sticky part of a band aid
Quote:
fig said: band aids also absorb moisture dont know if this is a good thing or not. i used them in my goggles when snowboarding to keep them from fogging up lol
thats a good point fig thank you for adding that. I assumed you used the whole band-aid in your goggles and mycomikey is saying use only the sticky part. should eliminate that problem. thanks guys.
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Doc_T
Random Dude
Registered: 03/06/09
Posts: 42,395
Loc: Colorado
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Re: Lids. [Re: Wimy]
#11351901 - 10/30/09 01:15 PM (14 years, 4 months ago) |
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Clear 'scotch' tape can be used to cover the innoc hole. Pull a little bit to get rid of what's been out in the air, and the rest is pretty sterile.
-------------------- You make it all possible. Doesn't it feel good?
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Lucienis
Learning Permit
Registered: 08/16/09
Posts: 98
Last seen: 11 years, 9 months
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Re: Lids. [Re: Doc_T]
#11351932 - 10/30/09 01:20 PM (14 years, 4 months ago) |
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If you are going to buy bandaids, look a few feet over and just buy micropore tape?
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Wimy
weiliiinmyyard
Registered: 08/25/09
Posts: 5,659
Loc: SE USA
Last seen: 3 years, 10 months
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Re: Lids. [Re: Doc_T]
#11352041 - 10/30/09 01:37 PM (14 years, 4 months ago) |
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thanks doc t you answered my next question.. because im realizing most bandaids have holes.
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Doc_T
Random Dude
Registered: 03/06/09
Posts: 42,395
Loc: Colorado
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Re: Lids. [Re: Wimy]
#11352232 - 10/30/09 02:12 PM (14 years, 4 months ago) |
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I know it sounds sketchy at first, but it's true. Tape, plastic wrap, all that kind of stuff is basically sterile. The top doesn't matter, just the sticky side. You get pretty good at lifting the foil and sticking the tape in one motion.
But, RTV injection ports are so much nicer and easier. And a polyfil filter.
-------------------- You make it all possible. Doesn't it feel good?
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13shrooms
Lightning Shaman
Registered: 01/01/09
Posts: 26,719
Loc: IN ETHERS TORSION FIELD
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Re: Lids. [Re: Doc_T]
#11352287 - 10/30/09 02:20 PM (14 years, 4 months ago) |
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I have a 1/4" silicone injection port with breathable bandaids over 1/8" hole for gas exchange.
and polyfill with 1/4" center hole.
but my fav is synthetic filter discs with 3/16" holes.
-------------------- A M U Click here ^ for the AMU forum VVV AMUs Free Active/Edible/Exotic Spore Print or Syringe or Edible Culture Trade Thread VVV "Man is the sex organ of the machine world" ~ Marshall McLuhan
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myceleus_rex
seeker
Registered: 07/01/09
Posts: 1,581
Loc: alized
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Check out the lid thread in my sig. Silicone ports work great, are easy and cheap. Contrary to common opinion you don't even need RTV silicone, standard will handle PC temps just fine.
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Wimy
weiliiinmyyard
Registered: 08/25/09
Posts: 5,659
Loc: SE USA
Last seen: 3 years, 10 months
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seems like the whole silicone injection point is unnecessary.. see why cant you just use a polyfil filter for gas exchange and a bandaid when it seems to work fine.
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myceleus_rex
seeker
Registered: 07/01/09
Posts: 1,581
Loc: alized
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Re: Lids. [Re: Wimy]
#11352715 - 10/30/09 03:38 PM (14 years, 4 months ago) |
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Whatever works. More than one way to skin a cat. I just use it for the reasons I stated. It's reliable, easy and reusable.
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Wimy
weiliiinmyyard
Registered: 08/25/09
Posts: 5,659
Loc: SE USA
Last seen: 3 years, 10 months
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I didn't know they were reusable that's kind of cool. seems difficult to make them though. in comparison.
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Wimy
weiliiinmyyard
Registered: 08/25/09
Posts: 5,659
Loc: SE USA
Last seen: 3 years, 10 months
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Re: Lids. [Re: Wimy]
#11352802 - 10/30/09 03:53 PM (14 years, 4 months ago) |
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Heres another thing I don't want to start a whole new thread for- what are the benefits of using cakes, IF you are gonna crumble them? I realize alot of people have preferred methods but would it be just as effective to use RGS or birdseed for b+ cubensis?
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myceleus_rex
seeker
Registered: 07/01/09
Posts: 1,581
Loc: alized
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Re: Lids. [Re: Wimy]
#11352867 - 10/30/09 04:01 PM (14 years, 4 months ago) |
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Quote:
weiliiinmyyard said: I didn't know they were reusable that's kind of cool. seems difficult to make them though. in comparison.
I find them easy, and yes they handle multiple PC cycles. Just swab well with alcohol and flame the needle -good for many jabs.
Quote:
weiliiinmyyard said: Heres another thing I don't want to start a whole new thread for- what are the benefits of using cakes, IF you are gonna crumble them? I realize alot of people have preferred methods but would it be just as effective to use RGS or birdseed for b+ cubensis?
You're on the right track. I see no advantage to cakes unless you have no PC, which you do.
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13shrooms
Lightning Shaman
Registered: 01/01/09
Posts: 26,719
Loc: IN ETHERS TORSION FIELD
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I would stick to rgs or a blend of rgs and pine shavings for spawn jars, then as close as you can to natural habitat for substrates.
Im going through the same thing with liberty caps. link in my sig.
-------------------- A M U Click here ^ for the AMU forum VVV AMUs Free Active/Edible/Exotic Spore Print or Syringe or Edible Culture Trade Thread VVV "Man is the sex organ of the machine world" ~ Marshall McLuhan
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13shrooms
Lightning Shaman
Registered: 01/01/09
Posts: 26,719
Loc: IN ETHERS TORSION FIELD
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cakes are for doing many strains at once and getting prints efficiently. small projects.
but I guess a cake of rgs/verm/soil/grassroots could pin/fruit in a shotgun fc.?.
-------------------- A M U Click here ^ for the AMU forum VVV AMUs Free Active/Edible/Exotic Spore Print or Syringe or Edible Culture Trade Thread VVV "Man is the sex organ of the machine world" ~ Marshall McLuhan
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noobieshroomie
Back again
Registered: 07/18/08
Posts: 12,769
Loc: Not Too Sure
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i agree with the regular silicone it can with stand up to 400 degrees thats what i use on my lids
1/8th hole filled with silicone and 1/4 inch hole with tyvek held on by micropore works like a charm
-noobie-
-------------------- AMU Best Thread Ever CapZilla said: not sure what GE and FAE are but i should probably get some. Citric said: Your signature is wrong on colonization temps! GOOD JUDGMENT COMES FROM EXPERIENCE EXPERIENCE COMES FROM BAD JUDGMENT ROOM TEMP 70-75 IS BEST FOR COLONIZATION Thank you mycochef
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Wimy
weiliiinmyyard
Registered: 08/25/09
Posts: 5,659
Loc: SE USA
Last seen: 3 years, 10 months
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Quote:
13shrooms said: I would stick to rgs or a blend of rgs and pine shavings for spawn jars, then as close as you can to natural habitat for substrates.
Im going through the same thing with liberty caps. link in my sig.
funny that you say that. I couldnt think of a good first substrate to try for my other project so I went on a hike to the patch where I picked the mushroom, and dug up the soil exactly where i picked it from, grass roots, the dead wood in the ground, everything. boiled it in a pillowcase for an hour , cooled it in the freezer, and cased the (p weilii) colonized RGS/WBS in it in a tupperware container.
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Doc_T
Random Dude
Registered: 03/06/09
Posts: 42,395
Loc: Colorado
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Re: Lids. [Re: Wimy]
#11353303 - 10/30/09 05:05 PM (14 years, 4 months ago) |
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Quote:
weiliiinmyyard said: Heres another thing I don't want to start a whole new thread for- what are the benefits of using cakes, IF you are gonna crumble them?
They are easy, they are fast, they are efficient. If you are doing a lot of bulk, you'll need quarts of grain, but for a smaller project cakes are cool. And if that works out then- no PC.
Edit- also, cakes are versatile. Many species will grow on cakes, and often fruit, even if they won't grow on grains or coir.
-------------------- You make it all possible. Doesn't it feel good?
Edited by Doc_T (10/30/09 05:25 PM)
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