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Amazon Shop: Agar, Aquarium Heater, Petri Dish

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OfflineFoundas
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Registered: 09/02/09
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Petri dish condensation in incubator
    #11351496 - 10/30/09 02:18 PM (7 years, 11 months ago)

Hi,

i placed the inoculated petri dishes in the incubator using 2 containers and an aquarium heater. Temperature is at 26oC

I noticed though that all dishes created condensation on the lids where before, many of them were clear. Any advice or tricks on how to avoid this in the future and for the current petri dishes?

Thanks in advance


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Offlinecharvo
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Re: Petri dish condensation in incubator [Re: Foundas]
    #11354459 - 10/30/09 10:34 PM (7 years, 11 months ago)

Ya i dont use my incubator for plates and slants. I use the cupboards above my frig. because the temp is more stable. I have never had slow colonization. the temp is about 72F and very steady.


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OfflineGeorge Washington
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Re: Petri dish condensation in incubator [Re: Foundas]
    #11354576 - 10/30/09 10:58 PM (7 years, 11 months ago)

Store dishes upside down.


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Offlinecharvo
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Re: Petri dish condensation in incubator [Re: George Washington]
    #11354728 - 10/30/09 11:33 PM (7 years, 11 months ago)

i've heard about storing plates upside down. i personally have not tried it. I don't think that's a real good fix to the problem. fluctuating temps cause the condensation right. the only real way to fix the problem is to control the incubation temps. This can be very hard without expensive equipment. I have alittle condensation on my plates, but not enough to be a problem. My incubator temp fluctuates way to much for petri dishes.


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OfflineFoundas
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Re: Petri dish condensation in incubator [Re: charvo]
    #11355690 - 10/31/09 04:06 AM (7 years, 11 months ago)

Thanks for the replies guys.


Im watching the temp very closely in the incubator and it is fairly constant. 25-26oC

Would this condensation be a problem for the mycelium ? My bedroom closet is at 20oC. Is this temperature good enough for mycelium to grow?

Cheers


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Offlinesolumvita
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Re: Petri dish condensation in incubator [Re: Foundas]
    #11361697 - 11/01/09 11:46 AM (7 years, 11 months ago)

No problem with mycelium and condensation and 20 deg C is a good temp for growth.


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Offlinedkamp18
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Re: Petri dish condensation in incubator [Re: George Washington]
    #11364115 - 11/01/09 07:26 PM (7 years, 11 months ago)

Quote:

George Washington said:
Store dishes upside down.




that is the best way to avoid condensation, which can drip onto the mycelium and cause slower colonization.

just give the dishes a few days for the myc to get a hold of the agar, and turn them over, then even in the case of a little condensation it won't drip right back down onto the agar/mycelium


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OfflineFoundas
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Re: Petri dish condensation in incubator [Re: dkamp18]
    #11367527 - 11/02/09 09:23 AM (7 years, 11 months ago)

Thanks for all your answers guys
Last time i place the petri's in the incubator


Cheers


Edited by Foundas (11/02/09 09:24 AM)


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OfflineRogerRabbitM
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Re: Petri dish condensation in incubator [Re: George Washington]
    #11367541 - 11/02/09 09:35 AM (7 years, 11 months ago)

Quote:

George Washington said:
Store dishes upside down.




That is very bad advice and not based on science or mycology.

Condensation is caused by a temperature differential, so the cure is to stack the dishes in a vertical stack to eliminate the temp differential between the lid and outside air.

If you place them upside down, the water will seep out due to gravity, causing the agar to dry and the mycelium to suffer.  In addition, when you turn the dish right side up to open it, you flood the mycelium. 

Always store petri dishes right side up, and at normal room temperature.  Don't use an incubator.  If your room has large temperature variation between day and night, you can keep the dishes in the sleeve they were originally packed in and/or a shoe box to mitigate the temp swings.
RR


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Amazon Shop: Agar, Aquarium Heater, Petri Dish

Mushrooms, Mycology and Psychedelics >> Gourmet and Medicinal Mushrooms

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