Home | Community | Message Board


Everything Mushrooms
Please support our sponsors.

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Amazon Shop: ½ Pint Jars

Jump to first unread post. Pages: 1
Offlinedankster
Stranger
Registered: 07/04/09
Posts: 31
Last seen: 1 year, 10 months
some questions on LC and syringe sterilization
    #11347793 - 10/29/09 09:47 PM (8 years, 23 days ago)

I have just PC'd my jar for LC.  I have two syringes.  One is empty, one has two mL South American spores in it.  I have I am about to knock up the jar with 1 mL , and I am saving the 2nd mL in case something goes wrong with my LC.  I have 10 PF cakes to knock up once the LC is ready.  I would really like to squirt 2 mL LC into each jar to get a good start.  Would it be OK to suck up a full 10 mL syringe, inoculate 5 jars with 2 mL each, flame sterilize the needle, and then suck up another 10 mL for the last 5?  Do you think I should PC the syringe after I stick use up the first batch of LC?

Also, I sterilized the empty syringe wrapped in foil at the same time I PC's the LC solution.  If I leave it in a box wrapped in the same foil, do you think it should stay sterile during the time it takes (hopefully 5 - 7 days)for my LC to develop?


Post Extras: Print Post  Remind Me! Notify Moderator
InvisibleDoc_T
Random Dude
Male User Gallery


Folding@home Statistics
Registered: 03/06/09
Posts: 42,395
Loc: Colorado Flag
Re: some questions on LC and syringe sterilization [Re: dankster]
    #11347813 - 10/29/09 09:50 PM (8 years, 23 days ago)

I just don't understand what you are getting at.
If you are asking whether you can use 2mL of LC on a cake instead of 1 mL of spore solution, then yes is the answer.


--------------------
You make it all possible. Doesn't it feel good?


Post Extras: Print Post  Remind Me! Notify Moderator
Offlinefig
spore collector
Male User Gallery


Folding@home Statistics
Registered: 10/01/01
Posts: 723
Loc: usa east TN blue ridge mt...
Last seen: 1 year, 4 months
Re: some questions on LC and syringe sterilization [Re: Doc_T]
    #11347925 - 10/29/09 10:08 PM (8 years, 23 days ago)

man i have used one drop per jar before using lc and multi spore. you should be fine.
if u can see spores in the syringe theres thousands in there maybe more.
sounds like u are doing good though. keep us updated.


Post Extras: Print Post  Remind Me! Notify Moderator
Offlinedankster
Stranger
Registered: 07/04/09
Posts: 31
Last seen: 1 year, 10 months
Re: some questions on LC and syringe sterilization [Re: Doc_T]
    #11349130 - 10/30/09 12:38 AM (8 years, 23 days ago)

Quote:

Doc_T said:
I just don't understand what you are getting at.




Sorry, I guess what I wrote was a little confusing.  my questions are:

1.)would I risk contamination by drawing LC from the jar two separate times without PCing my syringe between them. 

2.)Will my sterilized syringe that is wrapped in tin foil and placed in a cardboard box remain sterile for up to a week while I wait for the LC to colonize.  Should I PC it again before filling it with LC?


Post Extras: Print Post  Remind Me! Notify Moderator
InvisibleDoc_T
Random Dude
Male User Gallery


Folding@home Statistics
Registered: 03/06/09
Posts: 42,395
Loc: Colorado Flag
Re: some questions on LC and syringe sterilization [Re: dankster]
    #11350278 - 10/30/09 08:31 AM (8 years, 23 days ago)

1) Yes. Some risk, it's not ideal.
2) No, should be ok.


--------------------
You make it all possible. Doesn't it feel good?


Post Extras: Print Post  Remind Me! Notify Moderator
Jump to top. Pages: 1

Amazon Shop: ½ Pint Jars

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

Similar ThreadsPosterViewsRepliesLast post
* Questionable LC Jar gema 1,471 5 12/03/09 09:19 AM
by stonesun
* a few questions, LC and Print Making Vs Potency. Phreakr 1,296 7 11/02/15 02:47 PM
by enemjay
* drying question, LC question doulovebeef 203 1 07/22/09 12:30 PM
by thatswhatshesaid
* A question about Microwave Sterilization. MegaShadow 853 5 12/15/03 06:11 PM
by MegaShadow
* Agar Work & Sterile Tek Questions - PC & Flame Sterilize + Antibotic Agar eltrkbrd 966 0 03/20/07 10:49 AM
by eltrkbrd
* LONG TERM STORAGE: LC->Vacutainer + sterile water Omnicracker 1,574 7 12/11/08 11:39 AM
by solumvita
* Question LC filters.
( 1 2 all )
Vyrk 3,539 24 12/19/07 11:21 AM
by Green420Thang
* My first attempts
( 1 2 3 4 5 6 all )
veda_sticks 12,581 116 10/07/07 06:31 AM
by veda_sticks

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Roadkill, Magash, Shroomism, george castanza, RogerRabbit, Citric, total, FooMan, 13shrooms, stonesun, cronicr, PussyFart, Tmethyl, Pastywhyte, bodhisatta
456 topic views. 28 members, 115 guests and 28 web crawlers are browsing this forum.
[ Toggle Favorite | Print Topic | Stats ]
Search this thread:
Shroom Supply
Please support our sponsors.

Copyright 1997-2017 Mind Media. Some rights reserved.

Generated in 0.028 seconds spending 0.007 seconds on 19 queries.