Home | Community | Message Board


Out-Grow.com - Mushroom Growing Kits & Supplies
Please support our sponsors.

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Amazon Shop: ½ Pint Jars

Jump to first unread post. Pages: 1
Offlinedankster
Stranger
Registered: 07/04/09
Posts: 31
Last seen: 2 years, 7 months
some questions on LC and syringe sterilization
    #11347793 - 10/29/09 09:47 PM (8 years, 9 months ago)

I have just PC'd my jar for LC.  I have two syringes.  One is empty, one has two mL South American spores in it.  I have I am about to knock up the jar with 1 mL , and I am saving the 2nd mL in case something goes wrong with my LC.  I have 10 PF cakes to knock up once the LC is ready.  I would really like to squirt 2 mL LC into each jar to get a good start.  Would it be OK to suck up a full 10 mL syringe, inoculate 5 jars with 2 mL each, flame sterilize the needle, and then suck up another 10 mL for the last 5?  Do you think I should PC the syringe after I stick use up the first batch of LC?

Also, I sterilized the empty syringe wrapped in foil at the same time I PC's the LC solution.  If I leave it in a box wrapped in the same foil, do you think it should stay sterile during the time it takes (hopefully 5 - 7 days)for my LC to develop?


Post Extras: Print Post  Remind Me! Notify Moderator
InvisibleDoc_T
Random Dude
Male User Gallery


Folding@home Statistics
Registered: 03/06/09
Posts: 42,395
Loc: Colorado Flag
Re: some questions on LC and syringe sterilization [Re: dankster]
    #11347813 - 10/29/09 09:50 PM (8 years, 9 months ago)

I just don't understand what you are getting at.
If you are asking whether you can use 2mL of LC on a cake instead of 1 mL of spore solution, then yes is the answer.


--------------------
You make it all possible. Doesn't it feel good?


Post Extras: Print Post  Remind Me! Notify Moderator
Offlinefig
spore collector
Male User Gallery


Folding@home Statistics
Registered: 10/01/01
Posts: 723
Loc: usa east TN blue ridge mt...
Last seen: 1 month, 21 days
Re: some questions on LC and syringe sterilization [Re: Doc_T]
    #11347925 - 10/29/09 10:08 PM (8 years, 9 months ago)

man i have used one drop per jar before using lc and multi spore. you should be fine.
if u can see spores in the syringe theres thousands in there maybe more.
sounds like u are doing good though. keep us updated.


Post Extras: Print Post  Remind Me! Notify Moderator
Offlinedankster
Stranger
Registered: 07/04/09
Posts: 31
Last seen: 2 years, 7 months
Re: some questions on LC and syringe sterilization [Re: Doc_T]
    #11349130 - 10/30/09 12:38 AM (8 years, 9 months ago)

Quote:

Doc_T said:
I just don't understand what you are getting at.




Sorry, I guess what I wrote was a little confusing.  my questions are:

1.)would I risk contamination by drawing LC from the jar two separate times without PCing my syringe between them. 

2.)Will my sterilized syringe that is wrapped in tin foil and placed in a cardboard box remain sterile for up to a week while I wait for the LC to colonize.  Should I PC it again before filling it with LC?


Post Extras: Print Post  Remind Me! Notify Moderator
InvisibleDoc_T
Random Dude
Male User Gallery


Folding@home Statistics
Registered: 03/06/09
Posts: 42,395
Loc: Colorado Flag
Re: some questions on LC and syringe sterilization [Re: dankster]
    #11350278 - 10/30/09 08:31 AM (8 years, 9 months ago)

1) Yes. Some risk, it's not ideal.
2) No, should be ok.


--------------------
You make it all possible. Doesn't it feel good?


Post Extras: Print Post  Remind Me! Notify Moderator
Jump to top. Pages: 1

Amazon Shop: ½ Pint Jars

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

Similar ThreadsPosterViewsRepliesLast post
* Wheat Straw Juice Questions? strain 1,146 6 02/27/02 10:07 PM
by strain
* Fruiting chamber sterility question. OLD_NEWBIE 1,744 4 03/24/02 04:45 PM
by DrAbeLincoln
* Re: Shroom Wiz Tek & Sterility Anonymous 1,575 9 12/02/99 11:26 PM
by Anonymous
* Bulk substrates sterilized in oven? rockclimber 2,057 4 01/23/03 03:56 AM
by rockclimber
* Sterile Syringes YellowSubmarine 584 3 11/26/02 06:05 AM
by pleezr
* Newbies Dumb Questions. OLD_NEWBIE 775 3 01/19/02 03:12 AM
by Mklangelo
* A couple questions MrPappagiorgio 534 2 05/09/01 12:34 AM
by sylo
* Quick Question cottlestonpie 291 2 01/28/03 08:42 AM
by cottlestonpie

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Roadkill, Magash, Shroomism, george castanza, RogerRabbit, Citric, total, FooMan, 13shrooms, stonesun, cronicr, PussyFart, Tmethyl, Pastywhyte, bodhisatta
488 topic views. 30 members, 133 guests and 24 web crawlers are browsing this forum.
[ Toggle Favorite | Print Topic | Stats ]
Search this thread:
Out-Grow.com - Mushroom Growing Kits & Supplies
Please support our sponsors.

Copyright 1997-2018 Mind Media. Some rights reserved.

Generated in 0.03 seconds spending 0.007 seconds on 19 queries.