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Amazon Shop: ½ Pint Jars, Agar

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InvisibleEpilson Lyrae
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ds
    #11345126 - 10/29/09 02:56 PM (8 years, 2 months ago)

Iint?


Edited by Epilson Lyrae (12/09/10 09:13 PM)


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Offlinebeto
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Re: calling all agar gods [Re: Epilson Lyrae]
    #11345182 - 10/29/09 03:04 PM (8 years, 2 months ago)

You are back at square one. If you are satisfied with the results, keep the isolate in a test tube inside your fridge. Otherwise u can repeat the process of isolating sectors using the new print or using the old print, that's up to you. You have millions of different types of dna on them to isolate.


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Offlinebw86
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Re: calling all agar gods [Re: beto]
    #11345249 - 10/29/09 03:14 PM (8 years, 2 months ago)

yep back to square one
if you want to save the sector
then make g2gs with your master jar.


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Convert to Grains Pf tek killed Jesus
...................................................AMU feel free to :pm:Agar to grain/ g2g transfer=unlimited mushrooms:hypnotoad:


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InvisibleDoc_T
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Re: calling all agar gods [Re: Epilson Lyrae]
    #11345334 - 10/29/09 03:28 PM (8 years, 2 months ago)

Quote:

gngsteroflove said:
Or are you back at square 1 with just another print?




At that point you are at Square Two, with a somewhat refined print. Lather, rinse, repeat for seven generations or more to stabilize a substrain to breed true.
Or so I've read.


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You make it all possible. Doesn't it feel good?


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OfflineLucienis
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Re: calling all agar gods [Re: Doc_T]
    #11345343 - 10/29/09 03:30 PM (8 years, 2 months ago)

Quote:

Doc_T said:
Quote:

gngsteroflove said:
Or are you back at square 1 with just another print?




At that point you are at Square Two, with a somewhat refined print. Lather, rinse, repeat for seven generations or more to stabilize a substrain to breed true.
Or so I've read.





Or make a master culture slant with the strain before you knock up the grain jar with it, and use the master slant to make LCs and keep using the same genetics if you like the consistency you're getting.
Agar lends it self to many options as I understand it all.


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InvisibleDoc_T
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Re: calling all agar gods [Re: Doc_T]
    #11345365 - 10/29/09 03:33 PM (8 years, 2 months ago)



--------------------
You make it all possible. Doesn't it feel good?


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OfflineLucienis
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Re: calling all agar gods [Re: Doc_T]
    #11345401 - 10/29/09 03:39 PM (8 years, 2 months ago)

Quote:

Doc_T said:
Found the link!
http://www.shroomery.org/forums/showflat.php/Number/10242476#10242476




God I get all giddy when Workman and RR talk science :wink:
Awesome find, thanks Doc!


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Amazon Shop: ½ Pint Jars, Agar

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