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 Damion5050
Mush Doctor
 
Registered: 05/01/08
Posts: 12,493
Loc: Lost In Translation ! 
Last seen: 3 years, 2 months
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 Spore Print To Agar. 
#11338548 - 10/28/09 01:28 PM (14 years, 5 months ago) |
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Well this is my first time doing print to agar.. I usually just due live mycelium to agar, but I got a print I want to transfer to agar.. Well I did this, my question is how long should it take to see some growth in the petri dishes using prints ?
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beto
Psiconauta
Registered: 09/04/09
Posts: 469
Loc: Paranoid City
Last seen: 5 months, 2 days
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Re: Spore Print To Agar. [Re: Damion5050]
#11338567 - 10/28/09 01:30 PM (14 years, 5 months ago) |
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from my experience from 2 to 15 days.
i have a dish with p. colombian spores that germinated in 2 days, the a+ albino germinated in 3 days.
the others took between 1 - 2 weeks tops.
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beto
Psiconauta
Registered: 09/04/09
Posts: 469
Loc: Paranoid City
Last seen: 5 months, 2 days
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Re: Spore Print To Agar. [Re: beto]
#11338584 - 10/28/09 01:33 PM (14 years, 5 months ago) |
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Ah, forgot to say how i do:
first i flame sterelize inoculation loop, cool it down in agar then softly touch the spore print, then touch agar. If u can see the spores u already have too much. Then i drop a drop of sterile water on the inoc point.
PS: i'm still in love with the japanese girl
Edited by beto (10/28/09 01:34 PM)
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Damion5050
Mush Doctor
Registered: 05/01/08
Posts: 12,493
Loc: Lost In Translation !
Last seen: 3 years, 2 months
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Re: Spore Print To Agar. [Re: beto]
#11338587 - 10/28/09 01:33 PM (14 years, 5 months ago) |
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Quote:
beto said:
from my experience from 2 to 15 days.
i have a dish with p. colombian spores that germinated in 2 days, the a+ albino germinated in 3 days.
the others took between 1 - 2 weeks tops.
Thanks.. I received a very nice print in the mail with a strain I have never worked with so I wanted to start work on it asap, but transferred it to agar instead of syringe, just to make sure it was clean.
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nexus1946
Rebel
Registered: 06/25/09
Posts: 2,315
Loc: In the moment
Last seen: 7 years, 9 months
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Re: Spore Print To Agar. [Re: Damion5050]
#11338649 - 10/28/09 01:41 PM (14 years, 5 months ago) |
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I usually scrape a little off with a scalpel directly to the agar. Then I cut the agar slightly, where the spores lay. I don't add water. IMO theres enough moisture in the agar. Should see growth within three to six days.
Good luck with it! 
--------------------
Gypsum/Drywall Tek
The Story Of Russell The Texas (Cube) Bear
 
The human race's prospects of survival were considerably better when we were defenceless against tigers than they are today when we have become defenceless against ourselves.
-Arnold J. Toynbee
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Damion5050
Mush Doctor
Registered: 05/01/08
Posts: 12,493
Loc: Lost In Translation !
Last seen: 3 years, 2 months
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Re: Spore Print To Agar. [Re: nexus1946]
#11338668 - 10/28/09 01:44 PM (14 years, 5 months ago) |
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Quote:
nexus1946 said:
I usually scrape a little off with a scalpel directly to the agar. Then I cut the agar slightly, where the spores lay. I don't add water. IMO theres enough moisture in the agar. Should see growth within three to six days.
Good luck with it!
All I did was scrap a little bit of the spores into the center of the dish and thats it cause there was extra moisture in the dish. I think it's been 3 days now.
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nexus1946
Rebel
Registered: 06/25/09
Posts: 2,315
Loc: In the moment
Last seen: 7 years, 9 months
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Re: Spore Print To Agar. [Re: Damion5050]
#11338722 - 10/28/09 01:51 PM (14 years, 5 months ago) |
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Quote:
Damion5050 said:
All I did was scrap a little bit of the spores into the center of the dish and thats it cause there was extra moisture in the dish. I think it's been 3 days now.
You should see growth soon.
I should have mentioned that I keep mine in the incubator at 27C/80F.
--------------------
Gypsum/Drywall Tek
The Story Of Russell The Texas (Cube) Bear
The human race's prospects of survival were considerably better when we were defenceless against tigers than they are today when we have become defenceless against ourselves.
-Arnold J. Toynbee
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Damion5050
Mush Doctor
Registered: 05/01/08
Posts: 12,493
Loc: Lost In Translation !
Last seen: 3 years, 2 months
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Re: Spore Print To Agar. [Re: nexus1946]
#11338735 - 10/28/09 01:53 PM (14 years, 5 months ago) |
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I keep mine at room temp 66-68 at night and 73-74 during the day..
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kechlesurf
Stranger
Registered: 11/30/07
Posts: 1,103
Last seen: 3 months, 4 days
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Re: Spore Print To Agar. [Re: beto]
#11338738 - 10/28/09 01:53 PM (14 years, 5 months ago) |
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Quote:
beto said:
PS: i'm still in love with the japanese girl
another reason why his posts are so damn nice
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nexus1946
Rebel
Registered: 06/25/09
Posts: 2,315
Loc: In the moment
Last seen: 7 years, 9 months
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Re: Spore Print To Agar. [Re: kechlesurf]
#11338756 - 10/28/09 01:56 PM (14 years, 5 months ago) |
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Quote:
kechlesurf said:
Quote:
beto said:
PS: i'm still in love with the japanese girl
another reason why his posts are so damn nice
Yeah she it hot!
--------------------
Gypsum/Drywall Tek
The Story Of Russell The Texas (Cube) Bear
The human race's prospects of survival were considerably better when we were defenceless against tigers than they are today when we have become defenceless against ourselves.
-Arnold J. Toynbee
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Damion5050
Mush Doctor
Registered: 05/01/08
Posts: 12,493
Loc: Lost In Translation !
Last seen: 3 years, 2 months
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Re: Spore Print To Agar. [Re: kechlesurf]
#11338759 - 10/28/09 01:56 PM (14 years, 5 months ago) |
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Quote:
kechlesurf said:
Quote:
beto said:
PS: i'm still in love with the japanese girl
another reason why his posts are so damn nice
Yeah she is pretty nice.. I got some naked girls in my photo's if you feel like browsing through it.
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nexus1946
Rebel
Registered: 06/25/09
Posts: 2,315
Loc: In the moment
Last seen: 7 years, 9 months
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Re: Spore Print To Agar. [Re: Damion5050]
#11338779 - 10/28/09 01:58 PM (14 years, 5 months ago) |
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Quote:
Damion5050 said:
I keep mine at room temp 66-68 at night and 73-74 during the day..
It may take longer at those temps but I'm not sure. 
--------------------
Gypsum/Drywall Tek
The Story Of Russell The Texas (Cube) Bear
The human race's prospects of survival were considerably better when we were defenceless against tigers than they are today when we have become defenceless against ourselves.
-Arnold J. Toynbee
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nexus1946
Rebel
Registered: 06/25/09
Posts: 2,315
Loc: In the moment
Last seen: 7 years, 9 months
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Re: Spore Print To Agar. [Re: Damion5050]
#11338791 - 10/28/09 01:59 PM (14 years, 5 months ago) |
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Quote:
Damion5050 said:
Quote:
kechlesurf said:
Quote:
beto said:
PS: i'm still in love with the japanese girl
another reason why his posts are so damn nice
Yeah she is pretty nice.. I got some naked girls in my photo's if you feel like browsing through it.
Didn't you post a link to her video a while back?
--------------------
Gypsum/Drywall Tek
The Story Of Russell The Texas (Cube) Bear
The human race's prospects of survival were considerably better when we were defenceless against tigers than they are today when we have become defenceless against ourselves.
-Arnold J. Toynbee
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Damion5050
Mush Doctor
Registered: 05/01/08
Posts: 12,493
Loc: Lost In Translation !
Last seen: 3 years, 2 months
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Re: Spore Print To Agar. [Re: nexus1946]
#11340425 - 10/28/09 05:28 PM (14 years, 5 months ago) |
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Quote:
nexus1946 said:
Quote:
Damion5050 said:
Quote:
kechlesurf said:
Quote:
beto said:
PS: i'm still in love with the japanese girl
another reason why his posts are so damn nice
Yeah she is pretty nice.. I got some naked girls in my photo's if you feel like browsing through it.
Didn't you post a link to her video a while back?
Yeah
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