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Amazon Shop: Agar, Petri Dish

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OfflineDamion5050
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Spore Print To Agar.
    #11338548 - 10/28/09 03:28 PM (7 years, 11 months ago)

Well this is my first time doing print to agar.. I usually just due live mycelium to agar, but I got a print I want to transfer to agar..  Well I did this, my question is how long should it take to see some growth in the petri dishes using prints ?


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Offlinebeto
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Re: Spore Print To Agar. [Re: Damion5050]
    #11338567 - 10/28/09 03:30 PM (7 years, 11 months ago)

from my experience from 2 to 15 days.

i have a dish with p. colombian spores that germinated in 2 days, the a+ albino germinated in 3 days.

the others took between 1 - 2 weeks tops.


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Offlinebeto
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Re: Spore Print To Agar. [Re: beto]
    #11338584 - 10/28/09 03:33 PM (7 years, 11 months ago)

Ah, forgot to say how i do:

first i flame sterelize inoculation loop, cool it down in agar then softly touch the spore print, then touch agar. If u can see the spores u already have too much. Then i drop a drop of sterile water on the inoc point.


PS: i'm still in love with the japanese girl


Edited by beto (10/28/09 03:34 PM)


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OfflineDamion5050
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Re: Spore Print To Agar. [Re: beto]
    #11338587 - 10/28/09 03:33 PM (7 years, 11 months ago)

Quote:

beto said:
from my experience from 2 to 15 days.

i have a dish with p. colombian spores that germinated in 2 days, the a+ albino germinated in 3 days.

the others took between 1 - 2 weeks tops.





Thanks.. I received a very nice print in the mail with a strain I have never worked with so I wanted to start work on it asap, but transferred it to agar instead of syringe, just to make sure it was clean.


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Offlinenexus1946
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Re: Spore Print To Agar. [Re: Damion5050]
    #11338649 - 10/28/09 03:41 PM (7 years, 11 months ago)

I usually scrape a little off with a scalpel directly to the agar. Then I cut the agar slightly, where the spores lay. I don't add water. IMO theres enough moisture in the agar. Should see growth within three to six days.

Good luck with it! :smile:


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The Story Of Russell The Texas (Cube) Bear



The human race's prospects of survival were considerably better when we were defenceless against tigers than they are today when we have become defenceless against ourselves.
-Arnold J. Toynbee


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OfflineDamion5050
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Re: Spore Print To Agar. [Re: nexus1946]
    #11338668 - 10/28/09 03:44 PM (7 years, 11 months ago)

Quote:

nexus1946 said:
I usually scrape a little off with a scalpel directly to the agar. Then I cut the agar slightly, where the spores lay. I don't add water. IMO theres enough moisture in the agar. Should see growth within three to six days.

Good luck with it! :smile:





All I did was scrap a little bit of the spores into the center of the dish and thats it cause there was extra moisture in the dish. I think it's been 3 days now.


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Offlinenexus1946
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Re: Spore Print To Agar. [Re: Damion5050]
    #11338722 - 10/28/09 03:51 PM (7 years, 11 months ago)

Quote:

Damion5050 said:
All I did was scrap a little bit of the spores into the center of the dish and thats it cause there was extra moisture in the dish. I think it's been 3 days now.





You should see growth soon.

I should have mentioned that I keep mine in the incubator at 27C/80F.


--------------------
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The Story Of Russell The Texas (Cube) Bear



The human race's prospects of survival were considerably better when we were defenceless against tigers than they are today when we have become defenceless against ourselves.
-Arnold J. Toynbee


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OfflineDamion5050
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Re: Spore Print To Agar. [Re: nexus1946]
    #11338735 - 10/28/09 03:53 PM (7 years, 11 months ago)

I keep mine at room temp 66-68 at night and 73-74 during the day..


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Offlinekechlesurf
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Re: Spore Print To Agar. [Re: beto]
    #11338738 - 10/28/09 03:53 PM (7 years, 11 months ago)

Quote:

beto said:

PS: i'm still in love with the japanese girl




another reason why his posts are so damn nice


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Offlinenexus1946
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Re: Spore Print To Agar. [Re: kechlesurf]
    #11338756 - 10/28/09 03:56 PM (7 years, 11 months ago)

Quote:

kechlesurf said:
Quote:

beto said:

PS: i'm still in love with the japanese girl




another reason why his posts are so damn nice




Yeah she it hot!


--------------------
Gypsum/Drywall Tek
The Story Of Russell The Texas (Cube) Bear



The human race's prospects of survival were considerably better when we were defenceless against tigers than they are today when we have become defenceless against ourselves.
-Arnold J. Toynbee


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OfflineDamion5050
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Re: Spore Print To Agar. [Re: kechlesurf]
    #11338759 - 10/28/09 03:56 PM (7 years, 11 months ago)

Quote:

kechlesurf said:
Quote:

beto said:

PS: i'm still in love with the japanese girl




another reason why his posts are so damn nice





Yeah she is pretty nice.. I got some naked girls in my photo's if you feel like browsing through it.


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Offlinenexus1946
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Re: Spore Print To Agar. [Re: Damion5050]
    #11338779 - 10/28/09 03:58 PM (7 years, 11 months ago)

Quote:

Damion5050 said:
I keep mine at room temp 66-68 at night and 73-74 during the day..




It may take longer at those temps but I'm not sure. :shrug:


--------------------
Gypsum/Drywall Tek
The Story Of Russell The Texas (Cube) Bear



The human race's prospects of survival were considerably better when we were defenceless against tigers than they are today when we have become defenceless against ourselves.
-Arnold J. Toynbee


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Offlinenexus1946
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Re: Spore Print To Agar. [Re: Damion5050]
    #11338791 - 10/28/09 03:59 PM (7 years, 11 months ago)

Quote:

Damion5050 said:
Quote:

kechlesurf said:
Quote:

beto said:

PS: i'm still in love with the japanese girl




another reason why his posts are so damn nice





Yeah she is pretty nice.. I got some naked girls in my photo's if you feel like browsing through it.




Didn't you post a link to her video a while back?


--------------------
Gypsum/Drywall Tek
The Story Of Russell The Texas (Cube) Bear



The human race's prospects of survival were considerably better when we were defenceless against tigers than they are today when we have become defenceless against ourselves.
-Arnold J. Toynbee


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OfflineDamion5050
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Re: Spore Print To Agar. [Re: nexus1946]
    #11340425 - 10/28/09 07:28 PM (7 years, 11 months ago)

Quote:

nexus1946 said:
Quote:

Damion5050 said:
Quote:

kechlesurf said:
Quote:

beto said:

PS: i'm still in love with the japanese girl




another reason why his posts are so damn nice





Yeah she is pretty nice.. I got some naked girls in my photo's if you feel like browsing through it.




Didn't you post a link to her video a while back?




Yeah


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Amazon Shop: Agar, Petri Dish

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