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InvisibleSlimz
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OK to melt the lid on an agar plate?
    #11331855 - 10/27/09 03:49 PM (8 years, 1 month ago)

ok so if i have an LC syringe and an agar plate i want to start with it

cant i just heat the needle and melt thru the lid

squirt my drop of LC out and then cover the hole with tape?


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Lazy Drywall Tek (no powdery mess)

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Invisiblenoobieshroomie
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Re: OK to melt the lid on an agar plate? [Re: Slimz]
    #11331895 - 10/27/09 03:54 PM (8 years, 1 month ago)

i dont see why not

  -noobie-


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CapZilla said:
not sure what GE and FAE are but i should probably get some.

Citric said:
Your signature is wrong on colonization temps!

GOOD JUDGMENT COMES FROM EXPERIENCE
EXPERIENCE COMES FROM BAD JUDGMENT

ROOM TEMP 70-75 IS BEST FOR COLONIZATION
Thank you mycochef


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InvisibleHerbal_Elixer
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Re: OK to melt the lid on an agar plate? [Re: noobieshroomie]
    #11331934 - 10/27/09 04:01 PM (8 years, 1 month ago)

I have thought about pre drilling the lids with a hole, and using a dab of silicon.. then I also thought about after it grew out, injecting sterile water in there.. shake it all about, and draw it back out.. ?

GL


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InvisibleSlimz
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Re: OK to melt the lid on an agar plate? [Re: Herbal_Elixer]
    #11331977 - 10/27/09 04:06 PM (8 years, 1 month ago)

Quote:

Herbal_Elixer said:
I also thought about after it grew out, injecting sterile water in there.. shake it all about, and draw it back out.. ?

GL



like a grain LC only with a dish.. good idea


--------------------
Lazy Drywall Tek (no powdery mess)

This series will blow your mind and confirm what you already know to be true.
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Best Thread Ever ! ! !

:pm: me if you have questions about lasers

Although i may advise others in a general way regarding all types of mushroom grows, and may even post question from other forums about growing "active" mushrooms, i only grow non-"active" mushrooms and edibles.

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InvisibleHerbal_Elixer
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Re: OK to melt the lid on an agar plate? [Re: Slimz]
    #11331980 - 10/27/09 04:07 PM (8 years, 1 month ago)

Yeah.. like that


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Edited by Herbal_Elixer (10/27/09 04:07 PM)


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Invisiblenoobieshroomie
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Re: OK to melt the lid on an agar plate? [Re: Herbal_Elixer]
    #11331987 - 10/27/09 04:08 PM (8 years, 1 month ago)

how would you keep the dish sterile
while drilling the hole

  -noobie-


--------------------
AMU

Best Thread Ever
CapZilla said:
not sure what GE and FAE are but i should probably get some.

Citric said:
Your signature is wrong on colonization temps!

GOOD JUDGMENT COMES FROM EXPERIENCE
EXPERIENCE COMES FROM BAD JUDGMENT

ROOM TEMP 70-75 IS BEST FOR COLONIZATION
Thank you mycochef


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InvisibleHerbal_Elixer
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Re: OK to melt the lid on an agar plate? [Re: noobieshroomie]
    #11332008 - 10/27/09 04:11 PM (8 years, 1 month ago)

in a g box.. sterile little bit, in new vise handle (also sterilized).. My idea any way..



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OfflineThyrax
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Re: OK to melt the lid on an agar plate? [Re: noobieshroomie]
    #11332026 - 10/27/09 04:13 PM (8 years, 1 month ago)

im using 125ml jar for my agar atm. I could work easy for what you wanna do.


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InvisibleSlimz
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Re: OK to melt the lid on an agar plate? [Re: Thyrax]
    #11332040 - 10/27/09 04:14 PM (8 years, 1 month ago)

yes but i like clear lids.


--------------------
Lazy Drywall Tek (no powdery mess)

This series will blow your mind and confirm what you already know to be true.
The Pharmacratic Inquisition
Best Thread Ever ! ! !

:pm: me if you have questions about lasers

Although i may advise others in a general way regarding all types of mushroom grows, and may even post question from other forums about growing "active" mushrooms, i only grow non-"active" mushrooms and edibles.

FeelFamily resident tech guru


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InvisibleHerbal_Elixer
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Re: OK to melt the lid on an agar plate? [Re: Thyrax]
    #11332043 - 10/27/09 04:15 PM (8 years, 1 month ago)

Quote:

Thyrax said:
im using 125ml jar for my agar atm. I could work easy for what you wanna do.





I just poured agar, 2 days ago, in a 1/2 pint with a drilled lid... hasnt contamed yet

:wink:


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Re: OK to melt the lid on an agar plate? [Re: Herbal_Elixer]
    #11332062 - 10/27/09 04:17 PM (8 years, 1 month ago)

Why go out of your way to mix and pour agar plates then? Just make a grain LC if you're not trying to isolate specific growth, which I think would be pretty hard done through the lid.

You could inject through the lid and try and suck up a plug of agar with the growth you're looking for, and transfer like that but I don't imagine that working all that well.

You can do agar transfers in a glovebox, as long as you seal them up with parafilm/press-n'-seal you wont get too many if any contams.


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OfflineThyrax
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Re: OK to melt the lid on an agar plate? [Re: Slimz]
    #11332083 - 10/27/09 04:20 PM (8 years, 1 month ago)

Quote:

Slimz said:
yes but i like clear lids.



yeah but you can use them only for the first them. When spore spore germinate you will need to transfer normaly anyway so normal petri dishes will be ok.


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Invisiblenoobieshroomie
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Re: OK to melt the lid on an agar plate? [Re: Thyrax]
    #11332103 - 10/27/09 04:22 PM (8 years, 1 month ago)

good idea Herbal

  -noobie-


--------------------
AMU

Best Thread Ever
CapZilla said:
not sure what GE and FAE are but i should probably get some.

Citric said:
Your signature is wrong on colonization temps!

GOOD JUDGMENT COMES FROM EXPERIENCE
EXPERIENCE COMES FROM BAD JUDGMENT

ROOM TEMP 70-75 IS BEST FOR COLONIZATION
Thank you mycochef


Post Extras: Print Post  Remind Me! Notify Moderator
InvisibleHerbal_Elixer
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Re: OK to melt the lid on an agar plate? [Re: Randomstickynote]
    #11332252 - 10/27/09 05:17 PM (8 years, 1 month ago)

Quote:

Randomstickynote said:
Why go out of your way to mix and pour agar plates then? Just make a grain LC if you're not trying to isolate specific growth, which I think would be pretty hard done through the lid.

You could inject through the lid and try and suck up a plug of agar with the growth you're looking for, and transfer like that but I don't imagine that working all that well.

You can do agar transfers in a glovebox, as long as you seal them up with parafilm/press-n'-seal you wont get too many if any contams.





say the growth is isolated.. do a final transfer of the isolate into the rigged dish.. Dish LC of the best growth..? I don't see why not.. if it doesn't contam in the process. Just like noccing up a grain jar with a wedge to make grain LC.. except that step skipped, not as exact.. but depending on how well you transfer what you want.. it could be pretty darn close. I have a PE I am working on.. all the newest transfers are 360 degrees of the same strong growth.. I would feel confident in any area of the growth.. or all of it (perfect guinea pig).


I am not saying.. that this is true or will work.. but I have thought about it. These are my thoughts.. and I will do it one day.  :hehehe:


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Invisiblewygram
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Re: OK to melt the lid on an agar plate? [Re: noobieshroomie]
    #11332279 - 10/27/09 05:24 PM (8 years, 1 month ago)

I'm not trying to be snide, but I have to ask what is the point of this? Why not just lift the lid and place a few drops quickly. It seems to me that this would be much faster and controlled than what you suggest.

Everything I can think of that you would do with the agar involves transferring it to an exposed petri dish or grain jar. If you don't want to open it now, what about later when you transfer away from it? Just a thought...


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InvisibleSlimz
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Re: OK to melt the lid on an agar plate? [Re: wygram]
    #11332284 - 10/27/09 05:25 PM (8 years, 1 month ago)

if i open it when its fully colonized.. im less worried about the agar getting contam


--------------------
Lazy Drywall Tek (no powdery mess)

This series will blow your mind and confirm what you already know to be true.
The Pharmacratic Inquisition
Best Thread Ever ! ! !

:pm: me if you have questions about lasers

Although i may advise others in a general way regarding all types of mushroom grows, and may even post question from other forums about growing "active" mushrooms, i only grow non-"active" mushrooms and edibles.

FeelFamily resident tech guru


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InvisibleHerbal_Elixer
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Re: OK to melt the lid on an agar plate? [Re: wygram]
    #11332308 - 10/27/09 05:31 PM (8 years, 1 month ago)

I don't know.. it sounds cool??

.. or maybe what you say is the point...

"Why not just lift the lid and place a few drops quickly"

"Everything I can think of that you would do with the agar involves transferring it to an exposed petri dish or grain jar"

.. so lets change that norm.. and not open it.. so its not exposed.. ever. All transfers (at this point) take place in sealed vessels, and not exposed..? Sound good..? Sound gay..?  "You never know if your LC is contamed until you use it." correct? You don't feel odds are better if the original noc came from a clean petri, and never got exposed?
I will find out, one day.


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Amazon Shop: ½ Pint Jars, Agar, Parafilm, Petri Dish

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