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Offlineerq
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Registered: 06/28/09
Posts: 31
Loc: 1200Block
Last seen: 1 year, 8 months
LC question
    #11327381 - 10/26/09 10:21 PM (7 years, 11 months ago)

I posted this question in my log but nobody answered.  For the LC do I just pick a fruit and get a clean (sterilized) slice from the stem and throw that into the liquid that you've made? What is the best part of the mushroom to use for LC? Also, how soon do you have to do that once you pick the fruit (shelf life)?

Thanks guys and ladies


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InvisibleSlimz
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Re: LC question [Re: erq]
    #11327407 - 10/26/09 10:24 PM (7 years, 11 months ago)

best is to do a biopsy og the stem..

clean the stem with alcohol and shove a syringe full of sterile water thru the stem.. this will cause a small biopsy (core sample) to get stuck in the needle and then you force that into the LC..

DO NOT flame the needle with the biopsy in it


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Lazy Drywall Tek (no powdery mess)

This series will blow your mind and confirm what you already know to be true.
The Pharmacratic Inquisition
Best Thread Ever ! ! !

:pm: me if you have questions about lasers

Although i may advise others in a general way regarding all types of mushroom grows, and may even post question from other forums about growing "active" mushrooms, i only grow non-"active" mushrooms and edibles.

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Invisiblenoobieshroomie
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Re: LC question [Re: erq]
    #11327417 - 10/26/09 10:25 PM (7 years, 11 months ago)

thats how you do it
i would use a piece of tissue about 1 inch
up from the bottom of the stem

also you want to do it as soon as you pick it

  -noobie-


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AMU

Best Thread Ever
CapZilla said:
not sure what GE and FAE are but i should probably get some.

Citric said:
Your signature is wrong on colonization temps!

GOOD JUDGMENT COMES FROM EXPERIENCE
EXPERIENCE COMES FROM BAD JUDGMENT

ROOM TEMP 70-75 IS BEST FOR COLONIZATION
Thank you mycochef


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OfflineThyrax
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Re: LC question [Re: noobieshroomie]
    #11327427 - 10/26/09 10:26 PM (7 years, 11 months ago)

best part is inside the mushroom, that way you are sure its sterile.


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InvisibleSlimz
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Re: LC question [Re: noobieshroomie]
    #11327428 - 10/26/09 10:26 PM (7 years, 11 months ago)

and BEFORE anything sporilates


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Lazy Drywall Tek (no powdery mess)

This series will blow your mind and confirm what you already know to be true.
The Pharmacratic Inquisition
Best Thread Ever ! ! !

:pm: me if you have questions about lasers

Although i may advise others in a general way regarding all types of mushroom grows, and may even post question from other forums about growing "active" mushrooms, i only grow non-"active" mushrooms and edibles.

FeelFamily resident tech guru


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InvisibleEpilson Lyrae
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Re: LC question [Re: erq]
    #11327430 - 10/26/09 10:26 PM (7 years, 11 months ago)

There are a lot of ways to do it. The best thing to do would be to look at the place at the top of the forum that says 'subforums' and look at the stuff under agar/liquid cultures. then come an ask what you need to know from there. It really will help you out.


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"Freedom is something that dies unless it's used." H.T.
I've come to believe that the heart is the filter of the enlightened mind. Epilson Lyrae


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InvisibleEpilson Lyrae
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Re: LC question [Re: Epilson Lyrae]
    #11327440 - 10/26/09 10:27 PM (7 years, 11 months ago)



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"Freedom is something that dies unless it's used." H.T.
I've come to believe that the heart is the filter of the enlightened mind. Epilson Lyrae


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Offlinecyb3rtr0n
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Re: LC question [Re: noobieshroomie]
    #11327465 - 10/26/09 10:30 PM (7 years, 11 months ago)

Any reason you two like using a tissue sample from the stem rather than the cap?


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InvisibleSlimz
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Re: LC question [Re: cyb3rtr0n]
    #11327478 - 10/26/09 10:31 PM (7 years, 11 months ago)

yep.. no spores in the stem..


--------------------
Lazy Drywall Tek (no powdery mess)

This series will blow your mind and confirm what you already know to be true.
The Pharmacratic Inquisition
Best Thread Ever ! ! !

:pm: me if you have questions about lasers

Although i may advise others in a general way regarding all types of mushroom grows, and may even post question from other forums about growing "active" mushrooms, i only grow non-"active" mushrooms and edibles.

FeelFamily resident tech guru


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Offlinecyb3rtr0n
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Re: LC question [Re: Slimz]
    #11327490 - 10/26/09 10:33 PM (7 years, 11 months ago)

:strokebeard:


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Offlineerq
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Registered: 06/28/09
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Re: LC question [Re: cyb3rtr0n]
    #11328653 - 10/27/09 01:19 AM (7 years, 11 months ago)

woah, thanks for the super quick replies =]
I guess I need to do more research than just using the search function.

I just picked some fresh fruits, but I dont have access to a pc until tomorrow morning, that should be fine right? (if I refrigerate)


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InvisibleSlimz
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Re: LC question [Re: erq]
    #11328658 - 10/27/09 01:20 AM (7 years, 11 months ago)

DO NOT PC THE FRUIT BODY ! ! ! ! ! ! !

thats WRONG WRONG WRONG ! ! !

take the biopsy from the fresh fruit


--------------------
Lazy Drywall Tek (no powdery mess)

This series will blow your mind and confirm what you already know to be true.
The Pharmacratic Inquisition
Best Thread Ever ! ! !

:pm: me if you have questions about lasers

Although i may advise others in a general way regarding all types of mushroom grows, and may even post question from other forums about growing "active" mushrooms, i only grow non-"active" mushrooms and edibles.

FeelFamily resident tech guru


Post Extras: Print Post  Remind Me! Notify Moderator
Offlinecyb3rtr0n
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Re: LC question [Re: Slimz]
    #11329044 - 10/27/09 02:33 AM (7 years, 11 months ago)

i think he ment to make the LC... 

:highfive:


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Offlineerq
Strangey pants


Registered: 06/28/09
Posts: 31
Loc: 1200Block
Last seen: 1 year, 8 months
Re: LC question [Re: cyb3rtr0n]
    #11330337 - 10/27/09 11:12 AM (7 years, 11 months ago)

haha, yeah slimz, that would kill the fruit. But thanks for the warning =]


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