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OfflineTHE CULTIVATOR
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mass contam contamintation cunts.
    #11326058 - 10/26/09 07:24 PM (8 years, 30 days ago)

hi had 50 pf jars they some after bout 4 days of innoc they all started smellin bit wierd i knew this ment contam but to my surprise they colonized good but after bout 50% colonization they had to be abandoned for 1 week on return they stunk terribly and all growth had stopped completely. when opened they felt ok not too dry, i put this down to fact it was a filthy flat(shroom room ok tho)

a week or so later another 50 cakes, new flat(clean), tyvekd over the lids and made incubator with large tyvek filter. SAME PROBLEM!!!
where the fuck did i go wrong?

all jars steamed 90min
have done the same thing more carelessly before with sucess,i dont understand.


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OfflineBrennus
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Re: mass contam contamintation cunts. [Re: THE CULTIVATOR]
    #11328336 - 10/27/09 12:30 AM (8 years, 30 days ago)

Bacterial contamination

Re-check sterilization of jars and also your sterile tek. Make sure you use latex gloves, tyvek wrist sleeves, and a glove box. Flame sterilize your syringe.


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Offlinecne9999
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Re: mass contam contamintation cunts. [Re: Brennus]
    #11329099 - 10/27/09 02:43 AM (8 years, 30 days ago)

Can you provide a few more details:

1)  How are you knocking them up?  Glovebox?  Open air?  Flow hood?  Etc...

2)  Spore syringes?  LC? Your spore or LC syringes? 

3) Are you wearing gloves? 

4) I assume we are talking about BRF, right? 

Any other details might help us help you better.


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OfflineTHE CULTIVATOR
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Re: mass contam contamintation cunts. [Re: cne9999]
    #11329983 - 10/27/09 09:21 AM (8 years, 30 days ago)

brf, no gloves, open air. MS.

and also growth just stops there is no mould but subtrate darkens.

the 2nd time we put tyvek under the lids so that no bad air could be drawn in frm the sides and also very quickly after inoculation another layer was taped over the holes.

thanx, any help much areciated 100 out of 100 caontams is ridiculous.


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Offlinecne9999
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Re: mass contam contamintation cunts. [Re: THE CULTIVATOR]
    #11330035 - 10/27/09 09:46 AM (8 years, 29 days ago)

Start with gloves, I usually clean my gloves with alcohol prior to knocking.

Don't do the free air.  Make a cheap glove box.  Search the forums, there are several good cheap glove box ideas.

If the growth stops, maybe the BRF is too packed.  I've seen that.

Any pics you could post?

Are you using a verm layer on top of the BRF?


--------------------
  Say hello to my little friend.    and...      From Sonny
     
AFOAF's Coffee Experiment  //  Rye Berry Cakes  //  My FOAF  //  I killed my baby Cubes!
Get a freaking camera!  //  Roscoe 'Fatty' Arbuckle  //  I love P.c.Burma!


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OfflineTHE CULTIVATOR
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Re: mass contam contamintation cunts. [Re: cne9999]
    #11330149 - 10/27/09 10:23 AM (8 years, 29 days ago)

Quote:

cne9999 said:
Start with gloves, I usually clean my gloves with alcohol prior to knocking.

Don't do the free air.  Make a cheap glove box.  Search the forums, there are several good cheap glove box ideas.

If the growth stops, maybe the BRF is too packed.  I've seen that.

Any pics you could post?

Are you using a verm layer on top of the BRF?



jars now emptied n cleaned but pics wouldnt be much good no water in bottom of jar, sub looks only slightly darker. its the smell man they stink.

the thing i dont understand is how many times i have suceeded being more creless than this. 100% contam rate is terrible.


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Offlinecne9999
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Re: mass contam contamintation cunts. [Re: THE CULTIVATOR]
    #11330190 - 10/27/09 10:37 AM (8 years, 29 days ago)

I know what you mean when you're riding the 100% contam train life sucks.

Are you following a specific TEK?  Maybe from the RR videos?  I suggest Roger Rabbit's videos on BRF and I sugeest following them to the letter.  It will work.

My only oher word of advice is to make a glove box and get some laytex or nitrile gloves and practice being CLEAN and sterile as possible during all phases.

And of course, waith for someone more experienced to chime in here too.  That always helps.  :smile:


--------------------
  Say hello to my little friend.    and...      From Sonny
     
AFOAF's Coffee Experiment  //  Rye Berry Cakes  //  My FOAF  //  I killed my baby Cubes!
Get a freaking camera!  //  Roscoe 'Fatty' Arbuckle  //  I love P.c.Burma!


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OfflineTHE CULTIVATOR
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Re: mass contam contamintation cunts. [Re: cne9999]
    #11330255 - 10/27/09 10:55 AM (8 years, 29 days ago)

thanx, wil defo try with a glovebox next time maybe i will only get 90% then lol.


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Offlinecne9999
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Re: mass contam contamintation cunts. [Re: THE CULTIVATOR]
    #11330296 - 10/27/09 11:03 AM (8 years, 29 days ago)

Good start.  Do you PC or steam?  That's another place the contams sneak in.  PC is better.


--------------------
  Say hello to my little friend.    and...      From Sonny
     
AFOAF's Coffee Experiment  //  Rye Berry Cakes  //  My FOAF  //  I killed my baby Cubes!
Get a freaking camera!  //  Roscoe 'Fatty' Arbuckle  //  I love P.c.Burma!


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OfflineTHE CULTIVATOR
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Re: mass contam contamintation cunts. [Re: cne9999]
    #11332608 - 10/27/09 06:11 PM (8 years, 29 days ago)

in the uk its hard to get a good pc for a reasonsable price.
but i did have a crappy pc(fitted 5 jars and no pressure guage) this was used for bout 20% of the jars, the rest steamed.


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Offlinecne9999
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Re: mass contam contamintation cunts. [Re: THE CULTIVATOR]
    #11332671 - 10/27/09 06:21 PM (8 years, 29 days ago)

OK.  That's a good start.


--------------------
  Say hello to my little friend.    and...      From Sonny
     
AFOAF's Coffee Experiment  //  Rye Berry Cakes  //  My FOAF  //  I killed my baby Cubes!
Get a freaking camera!  //  Roscoe 'Fatty' Arbuckle  //  I love P.c.Burma!


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Offlinecne9999
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Re: mass contam contamintation cunts. [Re: cne9999]
    #11332702 - 10/27/09 06:26 PM (8 years, 29 days ago)

Ok, now lets get you started with a cheap glove box.  Do a search of the forums for 'still air box' or 'glove box'.  You will see some really cheap one but they almost all do the trick.  I bet this is ht e#1 thing you can work on.

Hell, I've seen them made of cardboard (corrugated)!  Just get a box with a cover, cut two holes large enough to stick your arms in up to midway between wrist and elbow.  And get a cheap pair of nitrile or latex gloves.  That’s it!  Make sure the box is ‘see through’ on the top so you can see what you are doing inside it.

If I was able to give you any advice at all that I thought might help this one is it.

Seriously.

:biker:


--------------------
  Say hello to my little friend.    and...      From Sonny
     
AFOAF's Coffee Experiment  //  Rye Berry Cakes  //  My FOAF  //  I killed my baby Cubes!
Get a freaking camera!  //  Roscoe 'Fatty' Arbuckle  //  I love P.c.Burma!


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OfflineTHE CULTIVATOR
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Re: mass contam contamintation cunts. [Re: cne9999]
    #11333081 - 10/27/09 07:21 PM (8 years, 29 days ago)

i know how to make 1 just never needed to before now.
thanx.


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Offlinecne9999
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Re: mass contam contamintation cunts. [Re: THE CULTIVATOR]
    #11333091 - 10/27/09 07:22 PM (8 years, 29 days ago)

Cool.


--------------------
  Say hello to my little friend.    and...      From Sonny
     
AFOAF's Coffee Experiment  //  Rye Berry Cakes  //  My FOAF  //  I killed my baby Cubes!
Get a freaking camera!  //  Roscoe 'Fatty' Arbuckle  //  I love P.c.Burma!


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OfflineShroomFrog
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Re: mass contam contamintation cunts. [Re: THE CULTIVATOR]
    #11335629 - 10/28/09 01:14 AM (8 years, 29 days ago)

Quote:

THE CULTIVATOR said:
in the uk its hard to get a good pc for a reasonsable price.
but i did have a crappy pc(fitted 5 jars and no pressure guage) this was used for bout 20% of the jars, the rest steamed.





Does it use a weight to pressurize?  Most pc's or weights say somewhere on them what they pressurize to if they don't have a gauge.  If it's lower than 15psi then you can adjust time.  If it's under 10psi it's worth giving it a shot, but might take a long cook time to be effective.


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Yes, that string of words I arranged together does make sense!

Some mushrooms you only eat once.

Either you have morels or you don't  -Mycological humor


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