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InvisibleOverPopulation
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Registered: 09/26/09
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Still No Myc. Man *DELETED*
    #11325430 - 10/26/09 05:55 PM (8 years, 25 days ago)

Post deleted by sinsinati

Reason for deletion: old



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OfflineMcDude
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Re: Still No Myc. Man [Re: OverPopulation]
    #11325490 - 10/26/09 06:05 PM (8 years, 25 days ago)

What did you inoculate with? spore syringe from a vendor?


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InvisibleOverPopulation
Mycavelli


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Re: Still No Myc. Man [Re: McDude]
    #11325499 - 10/26/09 06:06 PM (8 years, 25 days ago)

Yeah spore syringe from sporeworks.


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OfflinePfffffff
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Re: Still No Myc. Man [Re: OverPopulation]
    #11325554 - 10/26/09 06:19 PM (8 years, 25 days ago)

What was your process?  How did u sterilize, how long did you let jars cool, etc?


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Re: Still No Myc. Man [Re: OverPopulation]
    #11325555 - 10/26/09 06:19 PM (8 years, 25 days ago)

I only really know what I've read. Didn't go deep enough(not past the dry verm) when innoculating? or the syringe got crazy hot somewhere along the way. I had 3 jars that sorta burnt leaving them sit on the bottom of the pc,and I had the substrate packed way to tight. They really couldn't grow but they did atleast colonize in the space the needle left in the substrate.


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OfflineCamKron
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Re: Still No Myc. Man [Re: McDude]
    #11325574 - 10/26/09 06:22 PM (8 years, 25 days ago)

what type of tape do you have over the holes on the jars? (gotta make sure its the proper MP tape)
some poeple dont even use tape, so whatever it is, you can take it off and wait another week or 2...
also, make sure they arnt in any sort of sealed container/incubator...fresh air is needed for colonization


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OfflineZed
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Re: Still No Myc. Man [Re: McDude]
    #11325580 - 10/26/09 06:23 PM (8 years, 25 days ago)

a nice closeup pic of the jars may help


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InvisibleOverPopulation
Mycavelli


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Re: Still No Myc. Man [Re: McDude]
    #11325588 - 10/26/09 06:24 PM (8 years, 25 days ago)

I boiled the jars in a pot for 1 hr. I elevated them off of the bottom about an inch. I know the needle went deep enough and I watched the spore solution run down the side of the jar. No tape on the holes. I was advised to take the tape off about a week ago


Edited by OverPopulation (10/26/09 06:27 PM)


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Invisiblesimbad42
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Re: Still No Myc. Man [Re: OverPopulation]
    #11325649 - 10/26/09 06:32 PM (8 years, 25 days ago)

could you see any spore's in the syringe?


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InvisibleOverPopulation
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Re: Still No Myc. Man [Re: OverPopulation]
    #11325653 - 10/26/09 06:34 PM (8 years, 25 days ago)



Yeah I saw the spores. This pic is before I took the tape off.


Edited by OverPopulation (10/26/09 06:36 PM)


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Re: Still No Myc. Man [Re: OverPopulation]
    #11325701 - 10/26/09 06:41 PM (8 years, 25 days ago)

I know that any shit covering the holes will slow it way the fuck down. A friend had the same issue with a foil lid.  You might give it just a bit longer IMO but I'll defer to some more experienced folks...


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Invisiblesimbad42
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Re: Still No Myc. Man [Re: OverPopulation]
    #11325733 - 10/26/09 06:44 PM (8 years, 25 days ago)

was that masking tape?:lmafo:how long did you leave it on for?
maybe that had some thing to do with it?
could just be bunk spores:confused:
but after 3 weeks. i would start over:2cents:


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InvisibleOverPopulation
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Re: Still No Myc. Man [Re: simbad42]
    #11325846 - 10/26/09 06:59 PM (8 years, 25 days ago)

Yeah it was masking tape. lol. I watched the RR video that said using masking tape was ok. This really sucks man because I've become obsessed with trying this new challenge(hobby) and it's not going so well.


Edited by OverPopulation (10/26/09 07:10 PM)


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OfflineZed
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Re: Still No Myc. Man [Re: OverPopulation]
    #11326050 - 10/26/09 07:23 PM (8 years, 25 days ago)

I dont think its  the tape, you should see myc growing well before the lack of gas exchange affects anything.

The colour in the pic looks ok too. When i get  bacterial contamination the substrate usually starts looking darker/slimier, but from the blurry pic it looks ok.

Im thinking its either too dry or too wet.

either way, 3 weeks is fair while to wait. i would knock more jars up if you have more and leave those be. If you dont have more jars then ditch those and try again.


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Re: Still No Myc. Man [Re: Zed]
    #11326108 - 10/26/09 07:28 PM (8 years, 25 days ago)

I am in a similar boat (kinda) at the moment. I used RR's videos as you did nocced em up 10/14. Thing is I did 8 with Brazilian and 2 with Cambodian. The Cambodian is colonizing normally both jars looking good with good myc growth. But the Brazilians have yet to show any gowth whatsoever. So I'm thinking bunk syringe on those Brazilians you may have a similar issue. Let them sit and hopefully something will pop up, I sure hope mine do.


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Re: Still No Myc. Man [Re: demonkinsin]
    #11326132 - 10/26/09 07:31 PM (8 years, 25 days ago)

you probably cooked your jars dude. i did that with 40 out of 72 jars...


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Re: Still No Myc. Man [Re: OverPopulation]
    #11326166 - 10/26/09 07:36 PM (8 years, 25 days ago)

Quote:

sinsinati said:
I noc'ed 10 pf jars over 3 weeks ago and used the pf tek. I been keeping them at room temp between 72 and 76 degrees in the dark and still no signs of myc. It's driving me crazy trying to figure out what went wrong. All advice or help is welcome.





MS can be a bitch. these jars were knocked up over a month ago... they still look the same. Used PE MS Syringe


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InvisibleOverPopulation
Mycavelli


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Re: Still No Myc. Man [Re: demonkinsin]
    #11326178 - 10/26/09 07:37 PM (8 years, 25 days ago)

Yeah I'm bout' ready to give up on those jars. Anybody have a print to donate to a good cause? I would pass on the deed once I have success. If so, PM me.


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Amazon Shop: ½ Pint Jars, Vermiculite

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