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CDS
Registered: 09/05/08
Posts: 301
Loc: Grey Area
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Corelation between agar mycelial growth and robust fruiting...
#11322651 - 10/26/09 04:01 AM (14 years, 5 months ago) |
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So is there any way to determine the fruiting characteristics of a strain started on agar from ms based upon visual indicators? Or is the only way to fruit it and see how it does...
I would assume that a perfectly circular, thick and bright isolated colony should fruit well- and that it's speed of colonization in relation to other similar samples should be a good indicator to its fruiting capabilities when compared to said other samples...
But I think I have read conflicting opinions on here over the years, and a recent search failed to locate anything relevant...
So whats the deal with that anyway?
-Thanks
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truskool
WTF?? FTW!!
Registered: 06/28/09
Posts: 11,194
Loc: Over the rainbow
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Re: Corelation between agar mycelial growth and robust fruiting... [Re: CDS]
#11322660 - 10/26/09 04:21 AM (14 years, 5 months ago) |
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try searching stamets super strain
-------------------- Ask AMU for the best mycology advice out there Roll it While I troll it. I don't mean to boast, but dam if I don't brag.
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Doc_T
Random Dude
Registered: 03/06/09
Posts: 42,395
Loc: Colorado
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Re: Corelation between agar mycelial growth and robust fruiting... [Re: truskool]
#11322712 - 10/26/09 05:26 AM (14 years, 5 months ago) |
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Colonization speed is just one of many factors. Your winning culture will colonize quickly, but that's just the qualifier- it then needs to fruit well, etc.
-------------------- You make it all possible. Doesn't it feel good?
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CDS
Registered: 09/05/08
Posts: 301
Loc: Grey Area
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Re: Corelation between agar mycelial growth and robust fruiting... [Re: Doc_T]
#11325215 - 10/26/09 03:18 PM (14 years, 5 months ago) |
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Yes but what do you look for to determine which ones will fruit best? If you had 10 petris and you could only fruit one, what would your reason be for choosing the one you did? (Thickness of the mycelial strands, vertical growth, shape, etc...)
-Thanks
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Doc_T
Random Dude
Registered: 03/06/09
Posts: 42,395
Loc: Colorado
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Re: Corelation between agar mycelial growth and robust fruiting... [Re: CDS]
#11325248 - 10/26/09 03:25 PM (14 years, 5 months ago) |
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Oh, no. If you have ten monocultures in petris, grow out ten tubs and check the results of each.
One way to get a head start is to use a tissue clone instead of spores to start your agar plate. That will reduce the amount of sectoring you have to deal with in order to get the isolated culture you want.
-------------------- You make it all possible. Doesn't it feel good?
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feelfunny
I am you
Registered: 03/11/09
Posts: 8,747
Loc: South
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Re: Corelation between agar mycelial growth and robust fruiting... [Re: CDS]
#11325255 - 10/26/09 03:26 PM (14 years, 5 months ago) |
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all of the above i would pick the left side on this
-------------------- IF A CAT AND DOG CAN GET ALONG WHY CANT EVERYONE ELSE? If the sky is falling, don't look up! Feel Family Founder. me if you are tired of hearing, "Use the search function".
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CDS
Registered: 09/05/08
Posts: 301
Loc: Grey Area
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Re: Corelation between agar mycelial growth and robust fruiting... [Re: Doc_T]
#11325334 - 10/26/09 03:37 PM (14 years, 5 months ago) |
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Quote:
Doc_T said: ...grow out ten tubs and check the results of each...
I would prefer to be able to pick the best one and grow all ten tubs from it, lol!
But you're saying that's not possible. That's what I was afraid of...
It's just so frustrating to have to trash a whole tub because it decided to take a shit rather than fruit...
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Doc_T
Random Dude
Registered: 03/06/09
Posts: 42,395
Loc: Colorado
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Re: Corelation between agar mycelial growth and robust fruiting... [Re: CDS]
#11325340 - 10/26/09 03:39 PM (14 years, 5 months ago) |
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That's why it's hard. If it was easy, everybody would do it. But if you start with a tissue clone, you'll likely get results, and then it's just a matter of picking the best from among the great tubs you have, and continuing with that one line of genetic info.
-------------------- You make it all possible. Doesn't it feel good?
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