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Invisibletruskool
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Transfer away from the contaims
    #11323301 - 10/26/09 12:00 PM (7 years, 11 months ago)

K so I got three plates that I would like you to look at and see if what I see is what you see.  Sorry the pics are crap.  They are two section plates a will be the top b will b the bottom then an hourly reference to see the sectors. for example a 1-2 would mean the top section sector is from one oclock(by my clock) to 2 oclock

APE

a1-3 a3-5 a5-8 a8-10 a10-1
b1130-230 b230-5 b5-8 b8-1130
Now for this plate I would think that a1-3 and b1130-230 are the most promising sectors

Next up an EQ plate

a1230-130 a130-3 a3-4 a4-530 a530-630NOTE a630-10 a10-1230
b12-3 b3-530 b530-630 b630-8 b8-9 b9-1030 b1030-12
I would think a3-4 is most promising and b8-9 b9-1030 b630-8 all look good to me with no real difference that I can see
NOTE I cut a transfer and moved to this dish

EQ second transfer

a :shrug: kinda looking like a monoculture
b1030-2 b2-5 b5-730 b730-1030

So what do you think are the best sectors and am I wrong in labeling them?


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InvisibleSlimz
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Re: Transfer away from the contaims [Re: truskool]
    #11323323 - 10/26/09 12:05 PM (7 years, 11 months ago)

sorry dude, the pics suk..

but on plate 1,looks like 3 oclock is best for section 1 and 2

on plate 2 looks like around 11 oclock in section 1 and no clue about section 2


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Edited by Slimz (10/26/09 12:06 PM)


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Invisibletruskool
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Re: Transfer away from the contaims [Re: Slimz]
    #11323339 - 10/26/09 12:07 PM (7 years, 11 months ago)

Yea i know the pics sux fucking cell fone posting these embarassing pics is making me want to go out and buy a half decent camera soon my friend soon


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Re: Transfer away from the contaims [Re: truskool]
    #11323458 - 10/26/09 12:33 PM (7 years, 11 months ago)

I can't make out anything from those pictures.  I'd suggest taking every growth from those dishes and transferring to new dishes.  You should be able to inoculate two or three sleeves of dishes.  Then, repeat the process in a few days.  Fruit every isolate to determine the best performer(s) and then go back to your well-marked dishes in the refrigerator to grab younger versions of the cultures that were best.  You can't eyeball the best cultures to transfer, especially on the first few transfers from spores. That's why I grab them all.
RR


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Invisibletruskool
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Re: Transfer away from the contaims [Re: RogerRabbit]
    #11323475 - 10/26/09 12:37 PM (7 years, 11 months ago)

How the hell do you "well mark a plate" ive been thinking of this but dont have any solid ideas about doing this.  Thanks for taking a look rr yea i need a camera.  I knew that it is best to transfer all sectors and then do as you suggest but dam I dont have that many dishes and not too much agar atm


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Invisibletruskool
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Re: Transfer away from the contaims [Re: truskool]
    #11323483 - 10/26/09 12:39 PM (7 years, 11 months ago)

Another plate with very promising growth!!!!!

:lmafo:


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Re: Transfer away from the contaims [Re: truskool]
    #11323488 - 10/26/09 12:40 PM (7 years, 11 months ago)

:lmafo::rofl2:

  -noobie-


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CapZilla said:
not sure what GE and FAE are but i should probably get some.

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Your signature is wrong on colonization temps!

GOOD JUDGMENT COMES FROM EXPERIENCE
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Offlinemilkman59
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Re: Transfer away from the contaims [Re: noobieshroomie]
    #11323727 - 10/26/09 01:27 PM (7 years, 11 months ago)

start with each transferable piece. label 1st one a1, second one b1, 3rd one c1, and 4th one d1

then when you transfer  from a1, label it  a1-2
so you'd know it was from a1-transfer2


--------------------
milkman does wikidzons... fresh start 10/26/09
prints sent out today sorry for delay.....10/27/09


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Invisibletruskool
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Re: Transfer away from the contaims [Re: milkman59]
    #11323750 - 10/26/09 01:31 PM (7 years, 11 months ago)

im just wondering what i can use for a marker to know where i started from


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Offlinemilkman59
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Re: Transfer away from the contaims [Re: milkman59]
    #11323770 - 10/26/09 01:37 PM (7 years, 11 months ago)

good question?


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prints sent out today sorry for delay.....10/27/09


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Offlinenastos
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Re: Transfer away from the contaims [Re: truskool]
    #11323840 - 10/26/09 01:50 PM (7 years, 11 months ago)

Quote:

truskool said:
Another plate with very promising growth!!!!!

:lmafo:



:puke:


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InvisibleHerbal_Elixer
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Re: Transfer away from the contaims [Re: truskool]
    #11323960 - 10/26/09 02:10 PM (7 years, 11 months ago)

Quote:

truskool said:
im just wondering what i can use for a marker to know where i started from





put a slight scratch (if the petri is plastic) on the top and bottom to line the lid and dish back up.. circle the areas with a marker on the lid, and number.. or mark 12 o'clock with a marker on the dish, and use your 1,2,3,4,.. o'clock method.. or mind meld with the rhizo.. or become one with the dish.. or ask it


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Invisibletruskool
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Re: Transfer away from the contaims [Re: Herbal_Elixer]
    #11323974 - 10/26/09 02:13 PM (7 years, 11 months ago)

Quote:

Herbal_Elixer said:
Quote:

truskool said:
im just wondering what i can use for a marker to know where i started from





put a slight scratch (if the petri is plastic) on the top and bottom to line the lid and dish back up.. circle the areas with a marker on the lid, and number.. or mark 12 o'clock with a marker on the dish, and use your 1,2,3,4,.. o'clock method.. or mind meld with the rhizo.. or become one with the dish.. or ask it



:lmafo:
yea the mind meld why didnt i just think of that.  uhm my problem with marking the top is that when i move the plate to do transfers im going to fuck up and not match it back up again and i dont tape my plates closed i could but i dont.  The scratch is a good idea maybe on the side of the plate


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Invisiblenoobieshroomie
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Re: Transfer away from the contaims [Re: truskool]
    #11323986 - 10/26/09 02:15 PM (7 years, 11 months ago)

put a mark on the side with a permanent marker
or nail polish

  -noobie-


--------------------
AMU

Best Thread Ever
CapZilla said:
not sure what GE and FAE are but i should probably get some.

Citric said:
Your signature is wrong on colonization temps!

GOOD JUDGMENT COMES FROM EXPERIENCE
EXPERIENCE COMES FROM BAD JUDGMENT

ROOM TEMP 70-75 IS BEST FOR COLONIZATION
Thank you mycochef


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InvisibleHerbal_Elixer
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Re: Transfer away from the contaims [Re: truskool]
    #11323993 - 10/26/09 02:15 PM (7 years, 11 months ago)

yeah.. scratch the lid and plate..  would be easy to line that back up.. it would only become hard to mark the lid if you did a bunch of transfers without catching up first.. but I don't know, I haven't got that technical yet.. right now, if I see no contams.. then..

:highfive:

we growing


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Re: Transfer away from the contaims [Re: Herbal_Elixer]
    #11324003 - 10/26/09 02:17 PM (7 years, 11 months ago)

truskool,should i start the thread now?

  -noobie-


--------------------
AMU

Best Thread Ever
CapZilla said:
not sure what GE and FAE are but i should probably get some.

Citric said:
Your signature is wrong on colonization temps!

GOOD JUDGMENT COMES FROM EXPERIENCE
EXPERIENCE COMES FROM BAD JUDGMENT

ROOM TEMP 70-75 IS BEST FOR COLONIZATION
Thank you mycochef


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Invisibletruskool
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Re: Transfer away from the contaims [Re: Herbal_Elixer]
    #11324025 - 10/26/09 02:19 PM (7 years, 11 months ago)

well those plates have only been opened in my gb and ive opened it a few times to check the progress. You see how nasty they are my house is fucking nasty i really hope this hepa filter helps

Noobie sure i can take some pics for it too that one and ill take another one rigth nw its sectored some more since the last pic :lmafo:


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Re: Transfer away from the contaims [Re: truskool]
    #11324042 - 10/26/09 02:22 PM (7 years, 11 months ago)

:pm: me

  -noobie-


--------------------
AMU

Best Thread Ever
CapZilla said:
not sure what GE and FAE are but i should probably get some.

Citric said:
Your signature is wrong on colonization temps!

GOOD JUDGMENT COMES FROM EXPERIENCE
EXPERIENCE COMES FROM BAD JUDGMENT

ROOM TEMP 70-75 IS BEST FOR COLONIZATION
Thank you mycochef


Post Extras: Print Post  Remind Me! Notify Moderator
Invisibletruskool
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Re: Transfer away from the contaims [Re: noobieshroomie]
    #11324087 - 10/26/09 02:31 PM (7 years, 11 months ago)

Whats a :pm:


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Ask  AMU    for    the  best    mycology    advice  out  there

  Roll it :burnone:While I troll it.  :maximumtrolling:

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Invisiblenoobieshroomie
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Re: Transfer away from the contaims [Re: truskool]
    #11324116 - 10/26/09 02:34 PM (7 years, 11 months ago)

:facepalm::lol:

  -noobie-


--------------------
AMU

Best Thread Ever
CapZilla said:
not sure what GE and FAE are but i should probably get some.

Citric said:
Your signature is wrong on colonization temps!

GOOD JUDGMENT COMES FROM EXPERIENCE
EXPERIENCE COMES FROM BAD JUDGMENT

ROOM TEMP 70-75 IS BEST FOR COLONIZATION
Thank you mycochef


Post Extras: Print Post  Remind Me! Notify Moderator
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Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

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