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OfflineFreedom
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Best way to transfer spores from faint print to agar?
    #11320118 - 10/25/09 08:07 PM (8 years, 1 month ago)

Hi, I have a faint lib print on printer paper.

i had three ideas:

1. just scrape the print and hope (assume?) many spores will land on the agar even though I can't see anything

2. cut a small piece of the paper and put that on the agar

3. wash a drop of water onto and off the paper, then put it on the agar

what should I do?


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Invisiblebadman
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Re: Best way to transfer spores from faint print to agar? [Re: Freedom]
    #11320127 - 10/25/09 08:10 PM (8 years, 1 month ago)

Can you get a swab or inculation loop?


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OfflineFreedom
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Re: Best way to transfer spores from faint print to agar? [Re: badman]
    #11320137 - 10/25/09 08:12 PM (8 years, 1 month ago)

damn that was a fast reply

maybe i've mistaken something for a long time. I thought you scraped the spores so they would fall on the agar, but using something to scrape the spores and then touch the agar - that must be the way to do it.

thanks


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OfflineDamion5050
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Re: Best way to transfer spores from faint print to agar? [Re: Freedom]
    #11320165 - 10/25/09 08:18 PM (8 years, 1 month ago)

What I do is use an exacto knife and flame sterilize it, then once I think it has cooled off enough, I just scape a little and I mean tiny bit of the print into an agar petri dish.  It really doesn't take alot. 

Also if you get contamination's, you just transfer healthy mycelium to a clean petri dish.


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Invisiblebadman
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Re: Best way to transfer spores from faint print to agar? [Re: Freedom]
    #11320166 - 10/25/09 08:18 PM (8 years, 1 month ago)

Yeh loops or swabs are the standard way bruvvaaa, trust.


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InvisibleSlimz
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Re: Best way to transfer spores from faint print to agar? [Re: badman]
    #11320170 - 10/25/09 08:19 PM (8 years, 1 month ago)

flick it..


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OfflineDamion5050
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Re: Best way to transfer spores from faint print to agar? [Re: Slimz]
    #11320197 - 10/25/09 08:23 PM (8 years, 1 month ago)

You can make a home made loop out of a pen and a piece of wire..  Just cut the wire about 2 inches long then stick both ends in the eraser end of the pencil, and BOOM a quick and cheap loop.


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OfflineBrennus
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Re: Best way to transfer spores from faint print to agar? [Re: Damion5050]
    #11320219 - 10/25/09 08:26 PM (8 years, 1 month ago)

Quote:

Damion5050 said:
What I do is use an exacto knife and flame sterilize it, then once I think it has cooled off enough, I just scape a little and I mean tiny bit of the print into an agar petri dish.  It really doesn't take alot. 

Also if you get contamination's, you just transfer healthy mycelium to a clean petri dish.




You can also cool your flame-sterilized tools in the receiving agar dish itself before making transfers or scraping spores.


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OfflineRogerRabbitM
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Re: Best way to transfer spores from faint print to agar? [Re: Brennus]
    #11320558 - 10/25/09 09:25 PM (8 years, 1 month ago)

Dip your inoculating loop into sterile water.  Place one drop of sterile water on the print, rub it around a very small area, and then transfer to agar.
RR


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OfflineBreakfast Crew
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Re: Best way to transfer spores from faint print to agar? [Re: RogerRabbit]
    #11320579 - 10/25/09 09:29 PM (8 years, 1 month ago)

I don't have a lot of experience with agar yet but definitely can just scrape your print then slice the center of the dish. I avoid water after many failed dishes.  I just can't afford a HEPA filter haha :shrug:


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