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OfflineTRIPPYCHIK
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Storing sterilized Agar.
    #1131847 - 12/11/02 09:14 AM (14 years, 5 months ago)

I was curous to know where, what temps and how long can you store sterilized PDA Agar? And If your able to reuse it, can you just microwave it for a couple of seconds or does it have to be PC'd again for a short period of time?
Also, can the agar be warm when transferring tissue or spores, or does it have to be really cool?

Thanks


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InvisibleJoshua
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Re: Storing sterilized Agar. [Re: TRIPPYCHIK]
    #1132005 - 12/11/02 10:39 AM (14 years, 5 months ago)

I store my agar in the fridge. Make sure to seal it well. I microwave the agar when I am ready to use it. I think PCing would be best though. I have had good results with this method.

Joshua


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OfflineTRIPPYCHIK
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Re: Storing sterilized Agar. [Re: Joshua]
    #1132063 - 12/11/02 10:55 AM (14 years, 5 months ago)

Cool I'll put it in the fridge. I have a Erlernmeyer screw closure flask closed tight. Should I PC for an hour at 15 psi again? Also, how long do you microwave Joshua?

Thanks a lot!


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InvisibleRoadkill
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Re: Storing sterilized Agar. [Re: TRIPPYCHIK]
    #1132097 - 12/11/02 11:08 AM (14 years, 5 months ago)

I just re-warm mine on the stove in a pan of boiling water.

I always wait til my agar is completely cool and solidified before using.




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OfflineTRIPPYCHIK
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Re: Storing sterilized Agar. [Re: Roadkill]
    #1132108 - 12/11/02 11:19 AM (14 years, 5 months ago)

Thanks Roadkill!


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InvisibleJoshua
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Re: Storing sterilized Agar. [Re: TRIPPYCHIK]
    #1132185 - 12/11/02 11:43 AM (14 years, 5 months ago)

I believe 15 min @ 15 psi should be fine in the PC. I usually microwave untill I see that the agar has completely liquified, I would guess 10-15 minutes.

Joshua


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Amazon Shop: Agar, Potato Dextrose

Mushrooms, Mycology and Psychedelics >> Advanced Mycology

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