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Amazon Shop: ½ Pint Jars, Agar, Brown Rice Flour, Microscope

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Offlineanonymoose
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Saving contaminated spore syringe?
    #11316206 - 10/25/09 03:43 AM (7 years, 11 months ago)

A friend recently purchased 4 spore syringes from a reputable shroomery sponsor(I won't mention the name, because they seem like a very reputable company).  She inoculated 12 BRF tek style jars using proper sterile procedures, and followed the mushroomvideos tek to a tee.  3 jars of each strain were inoculated.  After 3 weeks 9 are almost completely colonized, but the three treasure coasts show no signs of mycelium (except 1, but it was very small, and they stink of sour milk(clearly a bacteria infection)  Because the bacteria infected jars all came from 1 syringe (properly flamed between innocs) I have reason to believe the syringe itself was contaminated.

My friend was pretty excited about these treasure coasts, but she doesn't think she will have much luck with a contaminated syringe.

Is it possible to make a corn syrup LC to germinate the spores along with the bacteria, and then introduce hydrogen peroxide after one see some signs of mycelium strands to kill the bacteria?  If so, how much would one inject, and how long should they let the mycelium try to colonize before trying?  It would certainly be nice to save this strain.


Edited by anonymoose (10/25/09 04:07 AM)


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Re: Saving contaminated spore syringe? [Re: anonymoose]
    #11316254 - 10/25/09 03:52 AM (7 years, 11 months ago)

only way to save it would be agar if the syringe is indeed contam'd... id contact the site u got it from and tell em u see bacteria spores when viewing under a microscope.

if it is contam'd and it is from a reputable source I would say it was something u did and not the vendor. unless its some crappy site


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OfflineGuffeyJon
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Re: Saving contaminated spore syringe? [Re: anonymoose]
    #11316263 - 10/25/09 03:53 AM (7 years, 11 months ago)

try cakes they are more forgiving then grains, but honestly i'd re check your sterile procedures.


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OfflineGuffeyJon
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Re: Saving contaminated spore syringe? [Re: GuffeyJon]
    #11316272 - 10/25/09 03:55 AM (7 years, 11 months ago)

agar is used more from spore print then syringe, you might just end up throwing the thing away


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Re: Saving contaminated spore syringe? [Re: GuffeyJon]
    #11316274 - 10/25/09 03:55 AM (7 years, 11 months ago)

Quote:

GuffeyJon said:
try cakes they are more forgiving then grains, but honestly i'd re check your sterile procedures.





read more

Quote:

She inoculated 12 BRF tek style jars




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Re: Saving contaminated spore syringe? [Re: GuffeyJon]
    #11316280 - 10/25/09 03:56 AM (7 years, 11 months ago)

Quote:

GuffeyJon said:
agar is used more from spore print then syringe, you might just end up throwing the thing away





what are u talking about man... agar can be used with spore syringes prints lc whatever tissue anything... plz do not give advice if you dont know what u are talking about


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Invisibleavorg
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Re: Saving contaminated spore syringe? [Re: GuffeyJon]
    #11316283 - 10/25/09 03:57 AM (7 years, 11 months ago)

you could try agar. highly doubt the LC idea would work as most contams outpace mycelium in growth.

have gotten bad syringes myself before and couldn't save them even with agar, now I prefer prints.


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Amazon Shop: ½ Pint Jars, Agar, Brown Rice Flour, Microscope

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