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Amazon Shop: ½ Pint Jars, Agar

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Offlinefz6
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Storing Jars in the Fridge...how long do they stay vigorous?
    #11309884 - 10/24/09 04:27 AM (8 years, 8 months ago)

Just as the title says.  My friend noticed that after about 3-4 months the mycilium seemed a lot more sluggish, especially after 3 transfers.  Lets say my friend put his jar in the fridge after colonizing for 3 weeks to reach 100 percent colonization from spores.  If he left the jars in an air tight bag for 2 months how much of this vigorousness will be lost.  I guess my question is how long is 2 months in the refrigerator in room temp time.


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Offlinefundamentalchair
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Re: Storing Jars in the Fridge...how long do they stay vigorous? [Re: fz6]
    #11309945 - 10/24/09 04:47 AM (8 years, 8 months ago)

No GE = bad. Get a vent on those bad boys.

While I can't say for sure how they'll fare in a jar, slants (agar in a vial with mycelium) can keep upwards of 3-5 years. LC should be able to hold for at least a few months. I assume jars would be the same.

After doing g2g, mycellium becomes 'weaker'. There is a big word for it that I don't know how to spell. There is some debate on how many transfers this takes, but some claim to see negative impact after the 3rd g2g.


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Offlinefz6
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Re: Storing Jars in the Fridge...how long do they stay vigorous? [Re: fundamentalchair]
    #11311478 - 10/24/09 01:50 PM (8 years, 8 months ago)

So lets say my friend made some jars from spores.  Then he put it in the fridge as soon as it was 100%, I mean to the day and then left it there for 2 months.  If he then did g2g's with it would they still be as aggressive?  Lets assume that they were stored in black trash bags to block out the light in a fridge at 40 degrees too.


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Invisiblemister
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Re: Storing Jars in the Fridge...how long do they stay vigorous? [Re: fz6]
    #11311614 - 10/24/09 02:07 PM (8 years, 8 months ago)

I had jars in the fridge for 6 months and they came out of it like they were on fire. Colonized the new jars in a week.


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Offlinefz6
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Re: Storing Jars in the Fridge...how long do they stay vigorous? [Re: mister]
    #11311638 - 10/24/09 02:11 PM (8 years, 8 months ago)

Thats good to know.  My pal wants to knock up enough masters for 2 or 3 grows at a time, then throw them in the fridge for a later date.  Then he could just bust one out of the fridge and week later he would have his jars ready to go, and grow.

Would agar give you a longer storage time you think?


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OfflineBrennus
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Re: Storing Jars in the Fridge...how long do they stay vigorous? [Re: fundamentalchair]
    #11311729 - 10/24/09 02:25 PM (8 years, 8 months ago)

Quote:

fundamentalchair said:
After doing g2g, mycellium becomes 'weaker'. There is a big word for it that I don't know how to spell. There is some debate on how many transfers this takes, but some claim to see negative impact after the 3rd g2g.




Senescence. The cell lines start to get old and worn out. Generally, it's not recommended to do more than 2 or 3 transfers with the same cell line. This is why mycologists often start 'fresh' from master culture slants.


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Amazon Shop: ½ Pint Jars, Agar

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