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BDiddy
Stranger
Registered: 09/04/09
Posts: 66
Loc: Southeast
Last seen: 10 years, 22 days
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Just tried making a Penis Envy syringe.
#11301717 - 10/22/09 06:42 PM (14 years, 5 months ago) |
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I received a PE print/stamp from a fellow member and just tried making a syringe from it.
The problem with it is that it was a stamp on a piece of printing paper and of course being a PE stamp there wasn't many spores. I scraped and scraped and it didn't look to be any visible spores in the shot glass I was scrapping into. I went ahead and finished the syringe and squirted the paper clip I used to scrape with cause it looked like there were some spores on it.
I got the syringe out and looked. All I can really see are bits of paper fibers in it.
I was gonna use the syringe with agar to get a clean sample but I'm afraid that if I just used one drop on the agar that I may not get any spores on there!
What should I do?
-------------------- Oak Ridge and Cambodian Prints for trade! PM me!
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noobieshroomie
Back again
Registered: 07/18/08
Posts: 12,769
Loc: Not Too Sure
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Re: Just tried making a Penis Envy syringe. [Re: BDiddy]
#11301850 - 10/22/09 06:58 PM (14 years, 5 months ago) |
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leave the syringe standing up right(needle pointing down) for a day or two, this will let alot of the spores settle near the needle then just give the plunger just enough of a push to get the extra water out of the needle its self
then put a drop on agar
i would also do a few plates say 3-5 for good measure
-noobie-
-------------------- AMU Best Thread Ever CapZilla said: not sure what GE and FAE are but i should probably get some. Citric said: Your signature is wrong on colonization temps! GOOD JUDGMENT COMES FROM EXPERIENCE EXPERIENCE COMES FROM BAD JUDGMENT ROOM TEMP 70-75 IS BEST FOR COLONIZATION Thank you mycochef
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BDiddy
Stranger
Registered: 09/04/09
Posts: 66
Loc: Southeast
Last seen: 10 years, 22 days
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Re: Just tried making a Penis Envy syringe. [Re: noobieshroomie]
#11301890 - 10/22/09 07:02 PM (14 years, 5 months ago) |
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Quote:
noobieshroomie said: leave the syringe standing up right(needle pointing down) for a day or two, this will let alot of the spores settle near the needle then just give the plunger just enough of a push to get the extra water out of the needle its self
then put a drop on agar
i would also do a few plates say 3-5 for good measure
-noobie-
Great idea! Thanks! What do you think my chances are for getting some growth?
-------------------- Oak Ridge and Cambodian Prints for trade! PM me!
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noobieshroomie
Back again
Registered: 07/18/08
Posts: 12,769
Loc: Not Too Sure
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Re: Just tried making a Penis Envy syringe. [Re: BDiddy]
#11301924 - 10/22/09 07:06 PM (14 years, 5 months ago) |
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hard to say is/was the print clean is your sterile procedure good is your agar sterile too many things to consider
-noobie-
-------------------- AMU Best Thread Ever CapZilla said: not sure what GE and FAE are but i should probably get some. Citric said: Your signature is wrong on colonization temps! GOOD JUDGMENT COMES FROM EXPERIENCE EXPERIENCE COMES FROM BAD JUDGMENT ROOM TEMP 70-75 IS BEST FOR COLONIZATION Thank you mycochef
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Damion5050
Mush Doctor
Registered: 05/01/08
Posts: 12,493
Loc: Lost In Translation !
Last seen: 3 years, 2 months
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Re: Just tried making a Penis Envy syringe. [Re: noobieshroomie]
#11302038 - 10/22/09 07:21 PM (14 years, 5 months ago) |
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Yeah I would of just scraped it into some agar then went from there.. That IMO is the best way to work with a print. Just helps get away from all the contamination's.
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BDiddy
Stranger
Registered: 09/04/09
Posts: 66
Loc: Southeast
Last seen: 10 years, 22 days
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Re: Just tried making a Penis Envy syringe. [Re: Damion5050]
#11302561 - 10/22/09 08:42 PM (14 years, 5 months ago) |
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Quote:
Damion5050 said: Yeah I would of just scraped it into some agar then went from there.. That IMO is the best way to work with a print. Just helps get away from all the contamination's.
NOW you say that!! LOL
I had thought of that but wasn't sure it would work. I may do that with the remaining stamp if this syringe doesn't work out.
-------------------- Oak Ridge and Cambodian Prints for trade! PM me!
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Damion5050
Mush Doctor
Registered: 05/01/08
Posts: 12,493
Loc: Lost In Translation !
Last seen: 3 years, 2 months
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Re: Just tried making a Penis Envy syringe. [Re: BDiddy]
#11302908 - 10/22/09 09:31 PM (14 years, 5 months ago) |
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Yeah u transfer a print to agar let it grow and see what it looks like then transfer healthy mycelium to a new dish, and u do this over and over till u have a clean sample.
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noobieshroomie
Back again
Registered: 07/18/08
Posts: 12,769
Loc: Not Too Sure
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Re: Just tried making a Penis Envy syringe. [Re: Damion5050]
#11302920 - 10/22/09 09:33 PM (14 years, 5 months ago) |
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its a long process but sometimes its needed
-noobie-
-------------------- AMU Best Thread Ever CapZilla said: not sure what GE and FAE are but i should probably get some. Citric said: Your signature is wrong on colonization temps! GOOD JUDGMENT COMES FROM EXPERIENCE EXPERIENCE COMES FROM BAD JUDGMENT ROOM TEMP 70-75 IS BEST FOR COLONIZATION Thank you mycochef
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