Home | Community | Message Board


Mycohaus
Please support our sponsors.

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Amazon Shop: Agar

Jump to first unread post. Pages: 1
OfflineBDiddy
Stranger
Male


Registered: 09/04/09
Posts: 66
Loc: Southeast
Last seen: 4 years, 4 months
Just tried making a Penis Envy syringe.
    #11301717 - 10/22/09 08:42 PM (8 years, 8 months ago)

I received a PE print/stamp from a fellow member and just tried making a syringe from it.

The problem with it is that it was a stamp on a piece of printing paper and of course being a PE stamp there wasn't many spores. I scraped and scraped and it didn't look to be any visible spores in the shot glass I was scrapping into. I went ahead and finished the syringe and squirted the paper clip I used to scrape with cause it looked like there were some spores on it.

I got the syringe out and looked. All I can really see are bits of paper fibers in it. :frown:

I was gonna use the syringe with agar to get a clean sample but I'm afraid that if I just used one drop on the agar that I may not get any spores on there!

What should I do?


--------------------
Oak Ridge and Cambodian Prints for trade! PM me!


Post Extras: Print Post  Remind Me! Notify Moderator
Invisiblenoobieshroomie
Back again
Male User Gallery


Folding@home Statistics
Registered: 07/19/08
Posts: 12,769
Loc: Not Too Sure Flag
Re: Just tried making a Penis Envy syringe. [Re: BDiddy]
    #11301850 - 10/22/09 08:58 PM (8 years, 8 months ago)

leave the syringe standing up right(needle pointing down)
for a day or two, this will let alot of the spores settle near the needle
then just give the plunger just enough of a push to get the extra water out of the needle its self

then put a drop on agar

i would also do a few plates say 3-5 for good measure

    -noobie-


--------------------
AMU

Best Thread Ever
CapZilla said:
not sure what GE and FAE are but i should probably get some.

Citric said:
Your signature is wrong on colonization temps!

GOOD JUDGMENT COMES FROM EXPERIENCE
EXPERIENCE COMES FROM BAD JUDGMENT

ROOM TEMP 70-75 IS BEST FOR COLONIZATION
Thank you mycochef


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineBDiddy
Stranger
Male


Registered: 09/04/09
Posts: 66
Loc: Southeast
Last seen: 4 years, 4 months
Re: Just tried making a Penis Envy syringe. [Re: noobieshroomie]
    #11301890 - 10/22/09 09:02 PM (8 years, 8 months ago)

Quote:

noobieshroomie said:
leave the syringe standing up right(needle pointing down)
for a day or two, this will let alot of the spores settle near the needle
then just give the plunger just enough of a push to get the extra water out of the needle its self

then put a drop on agar

i would also do a few plates say 3-5 for good measure

    -noobie-




Great idea! Thanks! What do you think my chances are for getting some growth?


--------------------
Oak Ridge and Cambodian Prints for trade! PM me!


Post Extras: Print Post  Remind Me! Notify Moderator
Invisiblenoobieshroomie
Back again
Male User Gallery


Folding@home Statistics
Registered: 07/19/08
Posts: 12,769
Loc: Not Too Sure Flag
Re: Just tried making a Penis Envy syringe. [Re: BDiddy]
    #11301924 - 10/22/09 09:06 PM (8 years, 8 months ago)

hard to say
is/was the print clean
is your sterile procedure good
is your agar sterile
too many things to consider

  -noobie-


--------------------
AMU

Best Thread Ever
CapZilla said:
not sure what GE and FAE are but i should probably get some.

Citric said:
Your signature is wrong on colonization temps!

GOOD JUDGMENT COMES FROM EXPERIENCE
EXPERIENCE COMES FROM BAD JUDGMENT

ROOM TEMP 70-75 IS BEST FOR COLONIZATION
Thank you mycochef


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineDamion5050
Mush Doctor
Male User Gallery

Registered: 05/01/08
Posts: 12,487
Loc: Lost In Translation ! Flag
Last seen: 4 months, 23 days
Trusted Cultivator
Re: Just tried making a Penis Envy syringe. [Re: noobieshroomie]
    #11302038 - 10/22/09 09:21 PM (8 years, 8 months ago)

Yeah I would of just scraped it into some agar then went from there.. That IMO is the best way to work with a print.  Just helps get away from all the contamination's.


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineBDiddy
Stranger
Male


Registered: 09/04/09
Posts: 66
Loc: Southeast
Last seen: 4 years, 4 months
Re: Just tried making a Penis Envy syringe. [Re: Damion5050]
    #11302561 - 10/22/09 10:42 PM (8 years, 8 months ago)

Quote:

Damion5050 said:
Yeah I would of just scraped it into some agar then went from there.. That IMO is the best way to work with a print.  Just helps get away from all the contamination's.




NOW you say that!! LOL

I had thought of that but wasn't sure it would work. I may do that with the remaining stamp if this syringe doesn't work out.


--------------------
Oak Ridge and Cambodian Prints for trade! PM me!


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineDamion5050
Mush Doctor
Male User Gallery

Registered: 05/01/08
Posts: 12,487
Loc: Lost In Translation ! Flag
Last seen: 4 months, 23 days
Trusted Cultivator
Re: Just tried making a Penis Envy syringe. [Re: BDiddy]
    #11302908 - 10/22/09 11:31 PM (8 years, 8 months ago)

Yeah u transfer a print to agar let it grow and see what it looks like then transfer healthy mycelium to a new dish, and u do this over and over till u have a clean sample.


Post Extras: Print Post  Remind Me! Notify Moderator
Invisiblenoobieshroomie
Back again
Male User Gallery


Folding@home Statistics
Registered: 07/19/08
Posts: 12,769
Loc: Not Too Sure Flag
Re: Just tried making a Penis Envy syringe. [Re: Damion5050]
    #11302920 - 10/22/09 11:33 PM (8 years, 8 months ago)

its a long process but sometimes its needed

  -noobie-


--------------------
AMU

Best Thread Ever
CapZilla said:
not sure what GE and FAE are but i should probably get some.

Citric said:
Your signature is wrong on colonization temps!

GOOD JUDGMENT COMES FROM EXPERIENCE
EXPERIENCE COMES FROM BAD JUDGMENT

ROOM TEMP 70-75 IS BEST FOR COLONIZATION
Thank you mycochef


Post Extras: Print Post  Remind Me! Notify Moderator
Jump to top. Pages: 1

Amazon Shop: Agar

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

Similar ThreadsPosterViewsRepliesLast post
* Penis envy isolation part 2
( 1 2 3 4 5 6 7 all )
verum subsequentis 2,581 121 05/09/18 05:59 PM
by plurfekt
* In search of Penis envy info. Sillycybe 2,644 6 12/19/02 07:44 AM
by Sillycybe
* Success rate at first time making a sterile syring tekramrepus 3,071 9 09/18/02 10:23 PM
by PSILLYSIMON
* penis envy DinnerAndAMovie 640 3 12/12/02 10:47 AM
by Skikid16
* making own spore syringes AzulAgave 1,295 11 12/26/01 03:24 PM
by _JJ_
* Tips on making Syringes 00Nick00 3,279 19 08/29/02 03:58 AM
by goldtop
* how to make a sporesyringe (pictorial) Una 8,574 15 12/13/03 09:26 PM
by Psilocybeingzz
* syringe making questions.. StainItBlue 652 9 02/07/03 07:33 AM
by Bi0TeK

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Roadkill, Magash, Shroomism, george castanza, RogerRabbit, Citric, total, FooMan, 13shrooms, stonesun, cronicr, PussyFart, Tmethyl, Pastywhyte, bodhisatta
424 topic views. 10 members, 90 guests and 25 web crawlers are browsing this forum.
[ Toggle Favorite | Print Topic | Stats ]
Search this thread:
Vesp.co, Inc.
Please support our sponsors.

Copyright 1997-2018 Mind Media. Some rights reserved.

Generated in 0.032 seconds spending 0.007 seconds on 19 queries.