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OfflineBDiddy
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Just tried making a Penis Envy syringe.
    #11301717 - 10/22/09 08:42 PM (7 years, 11 months ago)

I received a PE print/stamp from a fellow member and just tried making a syringe from it.

The problem with it is that it was a stamp on a piece of printing paper and of course being a PE stamp there wasn't many spores. I scraped and scraped and it didn't look to be any visible spores in the shot glass I was scrapping into. I went ahead and finished the syringe and squirted the paper clip I used to scrape with cause it looked like there were some spores on it.

I got the syringe out and looked. All I can really see are bits of paper fibers in it. :frown:

I was gonna use the syringe with agar to get a clean sample but I'm afraid that if I just used one drop on the agar that I may not get any spores on there!

What should I do?


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Invisiblenoobieshroomie
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Re: Just tried making a Penis Envy syringe. [Re: BDiddy]
    #11301850 - 10/22/09 08:58 PM (7 years, 11 months ago)

leave the syringe standing up right(needle pointing down)
for a day or two, this will let alot of the spores settle near the needle
then just give the plunger just enough of a push to get the extra water out of the needle its self

then put a drop on agar

i would also do a few plates say 3-5 for good measure

    -noobie-


--------------------
AMU

Best Thread Ever
CapZilla said:
not sure what GE and FAE are but i should probably get some.

Citric said:
Your signature is wrong on colonization temps!

GOOD JUDGMENT COMES FROM EXPERIENCE
EXPERIENCE COMES FROM BAD JUDGMENT

ROOM TEMP 70-75 IS BEST FOR COLONIZATION
Thank you mycochef


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OfflineBDiddy
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Registered: 09/04/09
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Re: Just tried making a Penis Envy syringe. [Re: noobieshroomie]
    #11301890 - 10/22/09 09:02 PM (7 years, 11 months ago)

Quote:

noobieshroomie said:
leave the syringe standing up right(needle pointing down)
for a day or two, this will let alot of the spores settle near the needle
then just give the plunger just enough of a push to get the extra water out of the needle its self

then put a drop on agar

i would also do a few plates say 3-5 for good measure

    -noobie-




Great idea! Thanks! What do you think my chances are for getting some growth?


--------------------
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Invisiblenoobieshroomie
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Re: Just tried making a Penis Envy syringe. [Re: BDiddy]
    #11301924 - 10/22/09 09:06 PM (7 years, 11 months ago)

hard to say
is/was the print clean
is your sterile procedure good
is your agar sterile
too many things to consider

  -noobie-


--------------------
AMU

Best Thread Ever
CapZilla said:
not sure what GE and FAE are but i should probably get some.

Citric said:
Your signature is wrong on colonization temps!

GOOD JUDGMENT COMES FROM EXPERIENCE
EXPERIENCE COMES FROM BAD JUDGMENT

ROOM TEMP 70-75 IS BEST FOR COLONIZATION
Thank you mycochef


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineDamion5050
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Re: Just tried making a Penis Envy syringe. [Re: noobieshroomie]
    #11302038 - 10/22/09 09:21 PM (7 years, 11 months ago)

Yeah I would of just scraped it into some agar then went from there.. That IMO is the best way to work with a print.  Just helps get away from all the contamination's.


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OfflineBDiddy
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Registered: 09/04/09
Posts: 66
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Last seen: 3 years, 7 months
Re: Just tried making a Penis Envy syringe. [Re: Damion5050]
    #11302561 - 10/22/09 10:42 PM (7 years, 11 months ago)

Quote:

Damion5050 said:
Yeah I would of just scraped it into some agar then went from there.. That IMO is the best way to work with a print.  Just helps get away from all the contamination's.




NOW you say that!! LOL

I had thought of that but wasn't sure it would work. I may do that with the remaining stamp if this syringe doesn't work out.


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OfflineDamion5050
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Re: Just tried making a Penis Envy syringe. [Re: BDiddy]
    #11302908 - 10/22/09 11:31 PM (7 years, 11 months ago)

Yeah u transfer a print to agar let it grow and see what it looks like then transfer healthy mycelium to a new dish, and u do this over and over till u have a clean sample.


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Invisiblenoobieshroomie
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Re: Just tried making a Penis Envy syringe. [Re: Damion5050]
    #11302920 - 10/22/09 11:33 PM (7 years, 11 months ago)

its a long process but sometimes its needed

  -noobie-


--------------------
AMU

Best Thread Ever
CapZilla said:
not sure what GE and FAE are but i should probably get some.

Citric said:
Your signature is wrong on colonization temps!

GOOD JUDGMENT COMES FROM EXPERIENCE
EXPERIENCE COMES FROM BAD JUDGMENT

ROOM TEMP 70-75 IS BEST FOR COLONIZATION
Thank you mycochef


Post Extras: Print Post  Remind Me! Notify Moderator
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Amazon Shop: Agar

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