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Offlineanjay
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Pics! Myc or contam? If myc, then shake?
    #11290597 - 10/21/09 09:57 AM (8 years, 1 month ago)

Hey there everyone. I was determined to do my 1st grow without posting anything annoying, just gleaning the wealth of information here. But... I have noob-grower-contam-paranoia! Sorry. :blush:

I'm doing Mazatapecs (multispore syringe from a shroomery sponsor) on WBS in wide mouth pint jars. (I have a small pressure cooker) Used FooMan's WBS tek, tyvek filters. I was extremely careful with the whole process, loads of oust, bleach, rubbing alcohol, gloves, mask, 1.5 hr pressure cook, still air box, flaming hot needle cooled with alcohol, band-aids over innoc holes, blah blah blah. Still, I do not rule out the possibility that I am a fuck-up! Sooo.....

1. Is this contam? If so, what kind?

2. If it's myc, then should I shake the jars at this point? Some of them look around 30%, but they seem kind of thin.


Let me know what you think. :grin:





Additional info:

  • For some idiotic reason I shook the jars a bit after I knocked them up with about 1.75 ccs each.

  • This is the 6th day of incubation. First sign of "mycelium" was on the 4th day.

  • Since my house is frigid (about 58), I have them incubating at an average of 78-80 degrees.

  • All of the jars have similar growth, but this one is the most colonized. These two pics are of the same jar. The color of the stuff is pretty pure white, I'm just concerned with the transparency of it.

  • They don't smell bad. When I lift the foil off (which is VERY loose, btw) it just has a faint smell of WBS.



I appreciate any input. Thanks!


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Re: Pics! Myc or contam? If myc, then shake? [Re: anjay]
    #11291016 - 10/21/09 12:13 PM (8 years, 1 month ago)

Only 6 days from MS is incredibly fast could be a contam


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Offlineanjay
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Re: Pics! Myc or contam? If myc, then shake? [Re: Shea25]
    #11291157 - 10/21/09 12:47 PM (8 years, 1 month ago)

Yeah, I thought so as well.

However, that jar is in the warmest area of the incubator (hard to explain), and the other jars are only exhibiting about 1/3 of that amount of growth.

Still, I'm very suspicious. 

I'll throw up a couple of pictures of the other jars in a few.


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Re: Pics! Myc or contam? If myc, then shake? [Re: anjay]
    #11291249 - 10/21/09 01:05 PM (8 years, 1 month ago)

Here we go...

More pictures of my poor little pints.







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Re: Pics! Myc or contam? If myc, then shake? [Re: anjay]
    #11291273 - 10/21/09 01:10 PM (8 years, 1 month ago)

Shake it. Looks ok to me, but if it's a contam, it may declare itself if you shake.


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Re: Pics! Myc or contam? If myc, then shake? [Re: Doc_T]
    #11291290 - 10/21/09 01:13 PM (8 years, 1 month ago)

Your contamination is called " myc." :tongue:
Well its not smart shaking the jar after inoculation because the spores have to pair(rhyzo growth), but you are OK.


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Re: Pics! Myc or contam? If myc, then shake? [Re: Doc_T]
    #11291394 - 10/21/09 01:35 PM (8 years, 1 month ago)

First off I really appreciate you folks offering advice. =)

Quote:

Doc_T said:
Shake it. Looks ok to me, but if it's a contam, it may declare itself if you shake.




Ok.. Shook the really colonized jar. Saw some really bright white chunks. I'm assuming it will declare itself in the re-colonization rather than immediately..


Quote:

dstark said:
Your contamination is called " myc." :tongue:




YAY! :heart:  I hope you're right. It would be a major bummer for 7 jars to fail.

I was just so happy when I saw mycelium in one jar the other day that I was jumping around my apt squealing. My bf and cat think I'm nuts now.


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Re: Pics! Myc or contam? If myc, then shake? [Re: anjay]
    #11291450 - 10/21/09 01:46 PM (8 years, 1 month ago)

Quote:

anjay said:
First off I really appreciate you folks offering advice. =)

Quote:

Doc_T said:
Shake it. Looks ok to me, but if it's a contam, it may declare itself if you shake.




Ok.. Shook the really colonized jar. Saw some really bright white chunks. I'm assuming it will declare itself in the re-colonization rather than immediately..


Quote:

dstark said:
Your contamination is called " myc." :tongue:




YAY! :heart:  I hope you're right. It would be a major bummer for 7 jars to fail.

I was just so happy when I saw mycelium in one jar the other day that I was jumping around my apt squealing. My bf and cat think I'm nuts now.




Huh, this hobby is so much fun, right? :P

Between, what you plan to do after the jars fully colonize? bulk grow?


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Re: Pics! Myc or contam? If myc, then shake? [Re: dstark]
    #11291510 - 10/21/09 01:54 PM (8 years, 1 month ago)

Quote:

dstark said:
Huh, this hobby is so much fun, right? :P

Between, what you plan to do after the jars fully colonize? bulk grow?




Yeaaah! I thought making yogurt and beer and sourdough bread was fun, but this sure beats em all so far.

I plan on basically following Otto's bucket tek, only with slightly larger 5-gal buckets (I have tons of em). If all goes well I should have enough grain spawn for 2 buckets. Will be spawning to coir/coffee as in the tek. =)


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Re: Pics! Myc or contam? If myc, then shake? [Re: anjay]
    #11291547 - 10/21/09 02:00 PM (8 years, 1 month ago)

Sounds good! best of luck, dont forget posting a grow log with pics. :wink:


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Re: Pics! Myc or contam? If myc, then shake? [Re: dstark]
    #11291618 - 10/21/09 02:10 PM (8 years, 1 month ago)

Thanks! =)

I'll definitely throw together a log. I'm just afraid to get made fun of for my incubator.  :biggrin:

You'll see what I mean. :tongue:


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Re: Pics! Myc or contam? If myc, then shake? [Re: anjay]
    #11291705 - 10/21/09 02:23 PM (8 years, 1 month ago)

Huh you will be amazed of what people have made incubators from.
BTW 5 shrooms for you :smile:


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Re: Pics! Myc or contam? If myc, then shake? [Re: dstark]
    #11291902 - 10/21/09 02:53 PM (8 years, 1 month ago)

Whee! Thanks. I actually don't have enough posts to make a grow log, LOL.

I'd rate you right back but I can't do that yet either.  :facepalm:

But here's the incubator anyhow, haha...



The oven rack and takeout spacers are so that the jars don't rest directly on the hot surface of the yogurt maker thing. The bucket holds the heat in and keeps the jars inside in the dark.

I guess it works. :wink:


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Re: Pics! Myc or contam? If myc, then shake? [Re: anjay]
    #11296431 - 10/22/09 01:00 AM (8 years, 1 month ago)

Wow very nice i would say :smile: its very cool using whatever you have :thumbup:
You sure its not to hot? you have a thermometer? if not you better buy 1, it would help alot :yesnod: there are cheap once for only few bucks.


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~I Feel
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at Home~


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Re: Pics! Myc or contam? If myc, then shake? [Re: dstark]
    #11296602 - 10/22/09 01:21 AM (8 years, 1 month ago)

Ahhhh. Thats the good stuff you want there my freind...


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Re: Pics! Myc or contam? If myc, then shake? [Re: dstark]
    #11297885 - 10/22/09 08:50 AM (8 years, 1 month ago)

Quote:

dstark said:
Wow very nice i would say :smile: its very cool using whatever you have :thumbup:
You sure its not to hot? you have a thermometer? if not you better buy 1, it would help alot :yesnod: there are cheap once for only few bucks.




O thanks. :lol: I actually do have a thermometer -- it's for reptile terrariums. In my lovely diagram there you can see the wire running out from under the bucket which goes to the readout. Temp ranges between 76 and 80. I can control it with the foil, haha.



Thanks desertfungi! Good to hear. =)

Well since it's mycelium, I'm happy that it's going so fast. wheee


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Re: Pics! Myc or contam? If myc, then shake? [Re: anjay]
    #11301320 - 10/22/09 07:40 PM (8 years, 1 month ago)

Looking good. :thumbup:

You said you cool down your needle with alcohol after flaming when inoculating. Just a little bit of advice - this is bad sterile tek. Once a needle is red hot, there's no way to make it any more sterile. It's completely sterile - wiping that needle down with a dirty rag or paper towel soaked in isopropyl only adds another possible vector of contamination. The first few drops of solution will cool the needle.

Also, although I almost always advise against using incubators, they can be useful in an extremely cold house. Tone the temperature down a notch - you want room temperature, around 70-74 degrees. Remember, mycelium colonizing grains and bulk substrates generate their own heat. Those jars are a few degrees warmer on the inside than ambient, and temperatures above 80F are only beneficial to bacteria (particularly any latent endospores that survive 90 minutes @ 15 PSI) and competitor molds.

Good luck. :mushroom2:


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Re: Pics! Myc or contam? If myc, then shake? [Re: Brennus]
    #11304928 - 10/23/09 10:35 AM (8 years, 1 month ago)

Thanks!

You know, I thought the alcohol wiping thing was a little redundant. Thanks for the info. Will skip this step in the future.


I would love to be able to skip the incubator, but it's just not possible. It's basically the same temperature inside as it is outside here. I generally do not turn on my heat. Plus it's only October.  :blush: 

I've also noticed that as the mycelium colonizes the jars more aggressively, the temp in the incubator has spiked by about 4 degrees. I think this is because the jars take up almost the whole space in the incubator. I see what you mean about the heat generation. Crazy. Because of this I modified my incubator to drop the temp. I'll drop it a little more as per your advice. :grin:


Soooo all is going well. My so-called contamination has turned into thicker, whiter mycelium and I've already had to shake 2 of the 7 jars. Woohoo!

I can't believe it's working.  :rofl:


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Re: Pics! Myc or contam? If myc, then shake? [Re: anjay]
    #11304959 - 10/23/09 10:43 AM (8 years, 1 month ago)

Oh, and the jar that I shook two days ago is already well on its way to full colonization, so thanks Doc_T!


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Re: Pics! Myc or contam? If myc, then shake? [Re: anjay]
    #11304969 - 10/23/09 10:44 AM (8 years, 1 month ago)

:thumbup: Good luck, keep us posted!


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