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Invisibleartizen
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agar isolation help
    #11285172 - 10/20/09 03:38 PM (14 years, 5 months ago)

i'm going to do some transfers tonight from these.


can someone point out where the actual sectoring is, or point out areas you would take from? i don't really know what i'm looking for. btw the unlabeled dish is PB like the second.


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Invisibletruskool Happy Birthday!
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Re: agar isolation help [Re: artizen]
    #11285194 - 10/20/09 03:40 PM (14 years, 5 months ago)

they look like monocultures to me


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Invisibleartizen
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Re: agar isolation help [Re: truskool]
    #11285302 - 10/20/09 03:54 PM (14 years, 5 months ago)

ya think? it was the first transfer from some real ratty looking shit.  look at the first pic. you see the lines coming straight out from the center at like 12 and 2 o'clock. i thought that was sectoring.:shrug:


--------------------
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Re: agar isolation help [Re: artizen]
    #11285316 - 10/20/09 03:56 PM (14 years, 5 months ago)

maybe but it looks pretty steady:shrug: the rest look like mono's too tho


--------------------
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Re: agar isolation help [Re: truskool]
    #11285361 - 10/20/09 04:01 PM (14 years, 5 months ago)

so if it is a monoculture does it make a difference where you take it from? closer to the middle or edge?


--------------------
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HCA               AMU
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Re: agar isolation help [Re: artizen]
    #11285375 - 10/20/09 04:02 PM (14 years, 5 months ago)

i take sectors from wherever with a mono.  but dont know if there is a difference i would think it wouldnt matter but dont quote me


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Ask  AMU    for    the  best    mycology    advice  out  there

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OfflineThyrax
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Re: agar isolation help [Re: truskool]
    #11285619 - 10/20/09 04:41 PM (14 years, 5 months ago)

anywhere should work the same. And they all look iso to me except maybe the second one that may need one more transfer.


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Re: agar isolation help [Re: Thyrax]
    #11285639 - 10/20/09 04:43 PM (14 years, 5 months ago)

yea the second looks like two trnasfers onto the same dish.  Am i right OP thats why i didnt say anything


--------------------
Ask  AMU    for    the  best    mycology    advice  out  there

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Invisibleartizen
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Re: agar isolation help [Re: truskool]
    #11285912 - 10/20/09 05:24 PM (14 years, 5 months ago)

yeah, it fell off to one side and i accidentally cut it in half when i went to move it to the middle, so i just rolled with it.


--------------------
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