Home | Community | Message Board


Everything Mushrooms
Please support our sponsors.

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Amazon Shop: Agar

Jump to first unread post. Pages: 1
Invisibleartizen
JEFFERSONIAN
Male


Registered: 12/16/08
Posts: 1,996
Loc: HOME SWEET HOME
agar isolation help
    #11285172 - 10/20/09 05:38 PM (8 years, 9 months ago)

i'm going to do some transfers tonight from these.


can someone point out where the actual sectoring is, or point out areas you would take from? i don't really know what i'm looking for. btw the unlabeled dish is PB like the second.


--------------------
Formula: 0:levitate: Formula: 1                                                                                                     
HCA               AMU
IN  LOVING  MEMORY

1.6.1917  -  4.3.2010


Post Extras: Print Post  Remind Me! Notify Moderator
Invisibletruskool
WTF?? FTW!!
Male User Gallery


Registered: 06/28/09
Posts: 11,194
Loc: Over the rainbow
Re: agar isolation help [Re: artizen]
    #11285194 - 10/20/09 05:40 PM (8 years, 9 months ago)

they look like monocultures to me


--------------------
Ask  AMU    for    the  best    mycology    advice  out  there

  Roll it :burnone:While I troll it.  :maximumtrolling:

I don't mean to boast, but dam if I don't brag.


Post Extras: Print Post  Remind Me! Notify Moderator
Invisibleartizen
JEFFERSONIAN
Male


Registered: 12/16/08
Posts: 1,996
Loc: HOME SWEET HOME
Re: agar isolation help [Re: truskool]
    #11285302 - 10/20/09 05:54 PM (8 years, 9 months ago)

ya think? it was the first transfer from some real ratty looking shit.  look at the first pic. you see the lines coming straight out from the center at like 12 and 2 o'clock. i thought that was sectoring.:shrug:


--------------------
Formula: 0:levitate: Formula: 1                                                                                                     
HCA               AMU
IN  LOVING  MEMORY

1.6.1917  -  4.3.2010


Post Extras: Print Post  Remind Me! Notify Moderator
Invisibletruskool
WTF?? FTW!!
Male User Gallery


Registered: 06/28/09
Posts: 11,194
Loc: Over the rainbow
Re: agar isolation help [Re: artizen]
    #11285316 - 10/20/09 05:56 PM (8 years, 9 months ago)

maybe but it looks pretty steady:shrug: the rest look like mono's too tho


--------------------
Ask  AMU    for    the  best    mycology    advice  out  there

  Roll it :burnone:While I troll it.  :maximumtrolling:

I don't mean to boast, but dam if I don't brag.


Post Extras: Print Post  Remind Me! Notify Moderator
Invisibleartizen
JEFFERSONIAN
Male


Registered: 12/16/08
Posts: 1,996
Loc: HOME SWEET HOME
Re: agar isolation help [Re: truskool]
    #11285361 - 10/20/09 06:01 PM (8 years, 9 months ago)

so if it is a monoculture does it make a difference where you take it from? closer to the middle or edge?


--------------------
Formula: 0:levitate: Formula: 1                                                                                                     
HCA               AMU
IN  LOVING  MEMORY

1.6.1917  -  4.3.2010


Post Extras: Print Post  Remind Me! Notify Moderator
Invisibletruskool
WTF?? FTW!!
Male User Gallery


Registered: 06/28/09
Posts: 11,194
Loc: Over the rainbow
Re: agar isolation help [Re: artizen]
    #11285375 - 10/20/09 06:02 PM (8 years, 9 months ago)

i take sectors from wherever with a mono.  but dont know if there is a difference i would think it wouldnt matter but dont quote me


--------------------
Ask  AMU    for    the  best    mycology    advice  out  there

  Roll it :burnone:While I troll it.  :maximumtrolling:

I don't mean to boast, but dam if I don't brag.


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineThyrax
No way
Male User Gallery


Registered: 01/03/08
Posts: 1,068
Loc: Montreal, Quebec
Last seen: 7 years, 11 months
Re: agar isolation help [Re: truskool]
    #11285619 - 10/20/09 06:41 PM (8 years, 9 months ago)

anywhere should work the same. And they all look iso to me except maybe the second one that may need one more transfer.


--------------------


Post Extras: Print Post  Remind Me! Notify Moderator
Invisibletruskool
WTF?? FTW!!
Male User Gallery


Registered: 06/28/09
Posts: 11,194
Loc: Over the rainbow
Re: agar isolation help [Re: Thyrax]
    #11285639 - 10/20/09 06:43 PM (8 years, 9 months ago)

yea the second looks like two trnasfers onto the same dish.  Am i right OP thats why i didnt say anything


--------------------
Ask  AMU    for    the  best    mycology    advice  out  there

  Roll it :burnone:While I troll it.  :maximumtrolling:

I don't mean to boast, but dam if I don't brag.


Post Extras: Print Post  Remind Me! Notify Moderator
Invisibleartizen
JEFFERSONIAN
Male


Registered: 12/16/08
Posts: 1,996
Loc: HOME SWEET HOME
Re: agar isolation help [Re: truskool]
    #11285912 - 10/20/09 07:24 PM (8 years, 9 months ago)

yeah, it fell off to one side and i accidentally cut it in half when i went to move it to the middle, so i just rolled with it.


--------------------
Formula: 0:levitate: Formula: 1                                                                                                     
HCA               AMU
IN  LOVING  MEMORY

1.6.1917  -  4.3.2010


Post Extras: Print Post  Remind Me! Notify Moderator
Jump to top. Pages: 1

Amazon Shop: Agar

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

Similar ThreadsPosterViewsRepliesLast post
* Microscope, agar isolation, "print cleaning", tampanensis, flow hoods ADoobie 1,028 5 11/29/05 06:48 PM
by bongtoke
* agar isolations vs multispore = no contest for comparison? mavix 1,185 2 02/17/04 11:10 PM
by simplemachine
* Question about agar nd595 2,508 18 03/10/04 06:35 PM
by Anonymous
* rhizomorphic isolate going back to linear / tomentose ohmatic 2,750 15 01/19/05 03:47 PM
by tripndicular
* myc isolation through LC HidingInPlainSight 440 4 05/23/05 11:41 AM
by IGnosticAbhorI
* How to make agar 2 agar transfer? esp 2,741 7 01/08/04 01:08 PM
by YidakiMan
* transferring agar meatman 1,043 3 10/02/02 03:13 PM
by tripndicular
* Effects of Multispore transfer on bulk yields Servator 1,027 4 11/14/02 01:01 AM
by DERRAYLD

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Roadkill, Magash, Shroomism, george castanza, RogerRabbit, Citric, total, FooMan, 13shrooms, stonesun, cronicr, PussyFart, Tmethyl, Pastywhyte, bodhisatta
352 topic views. 13 members, 82 guests and 14 web crawlers are browsing this forum.
[ Toggle Favorite | Print Topic | Stats ]
Search this thread:
Crestline Sales - MycoPath
Please support our sponsors.

Copyright 1997-2018 Mind Media. Some rights reserved.

Generated in 0.038 seconds spending 0.007 seconds on 19 queries.