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Offlinedeathblade
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Isolating P.Cube pics????
    #11271102 - 10/18/09 02:32 PM (8 years, 1 month ago)

Does anybody have any pics of agar dishes with P.Cube growth? I am trying this for the first time and the videos that I saw were using a different kind of mushroom.


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Offlinecharvo
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Re: Isolating P.Cube pics???? [Re: deathblade]
    #11271949 - 10/18/09 05:24 PM (8 years, 1 month ago)

the technique for isolating is the same rly no matter what species your working with. Here is photo of some sectors. You can plainly see the different types of growth.

your looking for the more aggressive growth to isolate. then you should fruit the isolate out. then observe how it performed on the substrate.



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Edited by charvo (10/18/09 05:28 PM)


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Offlinedeathblade
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Re: Isolating P.Cube pics???? [Re: charvo]
    #11273878 - 10/18/09 11:14 PM (8 years, 1 month ago)

Quote:

charvo said:
the technique for isolating is the same rly no matter what species your working with. Here is photo of some sectors. You can plainly see the different types of growth.

your looking for the more aggressive growth to isolate. then you should fruit the isolate out. then observe how it performed on the substrate.






In the case of the pic. You would take a sample from in between 630 and 1000 o clock correct?


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Offlinelibertaire
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Re: Isolating P.Cube pics???? [Re: deathblade]
    #11278410 - 10/19/09 06:19 PM (8 years, 1 month ago)

I think it depends.  I've heard that aggressive rhizomorphic growth does not mean big/dense fruits.  Does anyone know if this is true?  I forget where I've read this, but if I remember correctly, slower growth mean bigger and/or denser fruits, since the mycelium is taking it's sweet time to consolidate.  Which would explain why PE takes so long to fruit, but is considered the most potent.  Any thoughts?


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Invisibleanonjon
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Re: Isolating P.Cube pics???? [Re: libertaire]
    #11279853 - 10/19/09 09:52 PM (8 years, 1 month ago)

well, pe myc is very rhizo and spreads fast, it just takes a little longer to then fill out and get dense.

I think aggressive rhizo growth is just a sign of a vigorous strain. (with cubes that is)


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The above post is fictional, hypothetical, or downright nonsensical.


:moon:  :moon:  :moon:  :moon:    :moon:


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Offlinecharvo
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Re: Isolating P.Cube pics???? [Re: anonjon]
    #11286520 - 10/20/09 08:51 PM (8 years, 1 month ago)

Quote:

anonjon said:
well, pe myc is very rhizo and spreads fast, it just takes a little longer to then fill out and get dense.

I think aggressive rhizo growth is just a sign of a vigorous strain. (with cubes that is)





i agree with you.

Also i dont think just cause you have rhizomorphic growth means you'll have large/dense fruits. The whole reason for conducting mini trails with isolates is to evaluate the growth characteristics. you should isolate the vigorous growth. It can be valuable if the isolate works out for you. I would try and work many different sectors of growth,and grow them all on the same substrate. see which one you think does better. look up biological efficiency To.


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Offlinedeathblade
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Re: Isolating P.Cube pics???? [Re: charvo]
    #11289046 - 10/21/09 01:25 AM (8 years, 1 month ago)

I need more petri dishes. There are a few things that I am gonna need before I succeed. I am gonna keep the culture alive in a LC and will try to isolate it when I have enough supplies to do it properly.


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