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Offline5HTSynaptrip
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Mixing strains?
    #11268694 - 10/18/09 01:06 AM (8 years, 8 months ago)

So I'm at the point where I'd like to begin agar work and really narrow down a great performing strain.  Once you get that strain, in order to cross would you simply apply both mycelium to the same substrate to fruit?  Then collect spores, and take more isolates and fruit to test?


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InvisibleSlimz
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Re: Mixing strains? [Re: 5HTSynaptrip]
    #11268708 - 10/18/09 01:08 AM (8 years, 8 months ago)

start with both strains on the same agar plate


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Re: Mixing strains? [Re: Slimz]
    #11268721 - 10/18/09 01:10 AM (8 years, 8 months ago)

that is not going to work.  You would need to get a single spore to sprout before you could do anything


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Re: Mixing strains? [Re: truskool]
    #11268746 - 10/18/09 01:14 AM (8 years, 8 months ago)

not true..

you can cross strains, its been done..

PE6 is a cross


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Re: Mixing strains? [Re: Slimz]
    #11268759 - 10/18/09 01:16 AM (8 years, 8 months ago)

yes but you have to get a single spore to germ then work with that and only compatible genetics will cross


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Re: Mixing strains? [Re: Slimz]
    #11268790 - 10/18/09 01:20 AM (8 years, 8 months ago)

That's a pretty cool idea to try out, didn't know you could do this?


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Re: Mixing strains? [Re: Harri]
    #11268798 - 10/18/09 01:21 AM (8 years, 8 months ago)

there have been many ppl to try. The best way that i have heard of is getting a single monocultur and putting it in a jar then when the jar is 70% colonized innoculating with an lc of the second race you want to cross


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Re: Mixing strains? [Re: Slimz]
    #11268803 - 10/18/09 01:22 AM (8 years, 8 months ago)

search RRs and Workmans posts they have done it and posted how they did it. 
RR crossed Texas and PE to get PE6
Workman crossed PE with albino pf classic (I think) to get APE.


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OfflineThyrax
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Re: Mixing strains? [Re: truskool]
    #11268809 - 10/18/09 01:23 AM (8 years, 8 months ago)

Ive eared rr talking about snake venom in agar plate to cross pe and Tex.


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Re: Mixing strains? [Re: Thyrax]
    #11268814 - 10/18/09 01:24 AM (8 years, 8 months ago)

i know he used venom ot resurect redboy by crossing really old (20 yr) spores with PR


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Re: Mixing strains? [Re: Thyrax]
    #11268823 - 10/18/09 01:26 AM (8 years, 8 months ago)

rr also brought back 20 year old redboy spores with snake venom.thats how we have it know.


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Re: Mixing strains? [Re: whyblameus]
    #11268825 - 10/18/09 01:27 AM (8 years, 8 months ago)

damn you truskool you beet me.


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Offline5HTSynaptrip
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Re: Mixing strains? [Re: whyblameus]
    #11268832 - 10/18/09 01:29 AM (8 years, 8 months ago)

I just saw RR's post, was typing the wrong keywords.  So it looks like just straight agar it is.  Makes sense... I'm a bit sauced.


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Re: Mixing strains? [Re: whyblameus]
    #11268833 - 10/18/09 01:29 AM (8 years, 8 months ago)

lol yea snake venom i dont beleive works by itself i remeber that the way it works it breaks down the cell wall and makes the genetics easier to mix.  You still need to find compatible genes for it to work


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Re: Mixing strains? [Re: truskool]
    #11268849 - 10/18/09 01:33 AM (8 years, 8 months ago)

Quote:

You still need to find compatible genes for it to work





no matter the sub-strain name (PE, PESA, tex, etc) they are all P.Cubensis so compatible genes arent very hard to come by.

the snake venom was used to help revive OLD spores, good luck getting any if you wanted unless you milk your own rattle snakes. :eek:


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Re: Mixing strains? [Re: 13shrooms]
    #11268858 - 10/18/09 01:35 AM (8 years, 8 months ago)

Lol i know a place to purchase some


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Re: Mixing strains? [Re: truskool]
    #11268863 - 10/18/09 01:36 AM (8 years, 8 months ago)

thats right i think RR used pr to cross with the redboy. then back crossed it with the last of the redboy spores.making it 75% redboy 25%pr.


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Re: Mixing strains? [Re: whyblameus]
    #11268865 - 10/18/09 01:36 AM (8 years, 8 months ago)

Quote:

whyblameus said:
thats right i think RR used pr to cross with the redboy. then back crossed it with the last of the redboy spores.making it 75% redboy 25%pr.



:thumbup:


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Re: Mixing strains? [Re: truskool]
    #11268871 - 10/18/09 01:38 AM (8 years, 8 months ago)

Quote:

truskool said:
Lol i know a place to purchase some



ive seen a link that rr posted were he got his.


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Re: Mixing strains? [Re: whyblameus]
    #11268872 - 10/18/09 01:38 AM (8 years, 8 months ago)

yea that place you need like 5 background checks and shit one done by the police it is the perfect poison


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