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InvisibleSlimz
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G2G2G2G question
    #11264801 - 10/17/09 12:27 PM (8 years, 5 days ago)

Ok so i just did my first G2G (wbs) in my glovebox the other day and im hooked..

question is this..

once the target jars colonize, i can use them for more G2G right..

how many times can i do this?


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Re: G2G2G2G question [Re: Slimz]
    #11264807 - 10/17/09 12:29 PM (8 years, 5 days ago)

three


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Re: G2G2G2G question [Re: Slimz]
    #11264812 - 10/17/09 12:29 PM (8 years, 5 days ago)

You can do it as many times as you want, though it's not always a good idea to. Eventually, after a number of g2g's from the same genetics, your shrooms might not grow as much and they'll lose potency. It's always good to have new, fresh genetics every now and then.


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Re: G2G2G2G question [Re: Doc_T]
    #11264817 - 10/17/09 12:30 PM (8 years, 5 days ago)

really.. just 3...
what happens after that?


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Although i may advise others in a general way regarding all types of mushroom grows, and may even post question from other forums about growing "active" mushrooms, i only grow non-"active" mushrooms and edibles.

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Re: G2G2G2G question [Re: Slimz]
    #11264827 - 10/17/09 12:32 PM (8 years, 5 days ago)

"just" 3? That's 1,000 jars of spawn.


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Re: G2G2G2G question [Re: Doc_T]
    #11264848 - 10/17/09 12:36 PM (8 years, 5 days ago)

ok i think we are not thinking about the same thing..


if i have 1 colinized jar and i do a g2g to colonize 6 more jars then i take 1 of them and colonize 6 more jars, now im in my 3rd generation right?


--------------------
Lazy Drywall Tek (no powdery mess)

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Although i may advise others in a general way regarding all types of mushroom grows, and may even post question from other forums about growing "active" mushrooms, i only grow non-"active" mushrooms and edibles.

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Re: G2G2G2G question [Re: Slimz]
    #11264858 - 10/17/09 12:39 PM (8 years, 5 days ago)

Code:

master
|
/ /\ \
1 2 3 4
/ /\ \
1 2 3 4
/ /\ \
1 2 3 4

like that


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Lazy Drywall Tek (no powdery mess)

This series will blow your mind and confirm what you already know to be true.
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Best Thread Ever ! ! !

:pm: me if you have questions about lasers

Although i may advise others in a general way regarding all types of mushroom grows, and may even post question from other forums about growing "active" mushrooms, i only grow non-"active" mushrooms and edibles.

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Re: G2G2G2G question [Re: Slimz]
    #11264894 - 10/17/09 12:47 PM (8 years, 5 days ago)

Quote:

Slimz said:
ok i think we are not thinking about the same thing..


if i have 1 colinized jar and i do a g2g to colonize 6 more jars then i take 1 of them and colonize 6 more jars, now im in my 3rd generation right?




Second. Original jar = 'Generation' zero.
x6 = gen 1
x36 at gen 2
x 216 at gen 3


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Re: G2G2G2G question [Re: Doc_T]
    #11264907 - 10/17/09 12:51 PM (8 years, 5 days ago)

yeah but only if im using all my jars to colonize other jars..

i am small time over here..

i only have like 6-8 jars going at a time..


--------------------
Lazy Drywall Tek (no powdery mess)

This series will blow your mind and confirm what you already know to be true.
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Best Thread Ever ! ! !

:pm: me if you have questions about lasers

Although i may advise others in a general way regarding all types of mushroom grows, and may even post question from other forums about growing "active" mushrooms, i only grow non-"active" mushrooms and edibles.

FeelFamily resident tech guru


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Re: G2G2G2G question [Re: Doc_T]
    #11264921 - 10/17/09 12:53 PM (8 years, 5 days ago)

If one were to start with an LC, would that count as Generation Zero?


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Re: G2G2G2G question [Re: myceleus_rex]
    #11264933 - 10/17/09 12:56 PM (8 years, 5 days ago)

Quote:

myceleus_rex said:
If one were to start with an LC, would that count as Generation Zero?




Generally 'zero generation' is your spore generation. If you started the LC from spores, then yes. If it was started from a Grain LC or something, then no.

Grain LC is a convenient way to manage the expansion we are talking about. Make master syringes, generation zero from the original jars. Then let those jars colonize and spawn them. Then make a new batch of jars every 7 days or whatever works out for your schedule.

Edit- just to clarify, it's a nonstandard use of the word 'generation', which would more properly be used to describe the life cycle from spore print to spore print.
But we are talking about culture expansion and the potential for senescence.


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Re: G2G2G2G question [Re: Doc_T]
    #11264948 - 10/17/09 12:59 PM (8 years, 5 days ago)

ok thanks..

im new to the LC/G2G/Agar thing so im just getting the hang of it..


--------------------
Lazy Drywall Tek (no powdery mess)

This series will blow your mind and confirm what you already know to be true.
The Pharmacratic Inquisition
Best Thread Ever ! ! !

:pm: me if you have questions about lasers

Although i may advise others in a general way regarding all types of mushroom grows, and may even post question from other forums about growing "active" mushrooms, i only grow non-"active" mushrooms and edibles.

FeelFamily resident tech guru


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Re: G2G2G2G question [Re: Slimz]
    #11264960 - 10/17/09 01:01 PM (8 years, 5 days ago)

You also have to consider the number of transfers you have done on agar before you get to the jars, if you are doing it like that.
Each expansion counts toward your total lifespan of the culture. Try to get an early master culture.


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Re: G2G2G2G question [Re: Doc_T]
    #11264979 - 10/17/09 01:07 PM (8 years, 5 days ago)

im using a G started from spore at the moment..

made 3 G2G jars from it and dumped the rest in some coir to fruit.

i have another generation on the way tho and i think i will LC that one..


--------------------
Lazy Drywall Tek (no powdery mess)

This series will blow your mind and confirm what you already know to be true.
The Pharmacratic Inquisition
Best Thread Ever ! ! !

:pm: me if you have questions about lasers

Although i may advise others in a general way regarding all types of mushroom grows, and may even post question from other forums about growing "active" mushrooms, i only grow non-"active" mushrooms and edibles.

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Re: G2G2G2G question [Re: Slimz]
    #11265002 - 10/17/09 01:12 PM (8 years, 5 days ago)

What you need to do is figure a target colonization schedule. Whatever amount of jars you get from a single batch, that's your base amount. So if you do 10 quart jars, that's five shoebox tubs or two monotubs or whatever.
Then if you do one batch every ten days, that's 6 monotubs per month. Or 12 mini monos. Or however you tray it up.


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Re: G2G2G2G question [Re: Doc_T]
    #11265008 - 10/17/09 01:12 PM (8 years, 5 days ago)

Quote:

Doc_T said:
You also have to consider the number of transfers you have done on agar before you get to the jars, if you are doing it like that.
Each expansion counts toward your total lifespan of the culture. Try to get an early master culture.



:thumbup:

Though I can't agree with your definition of generations, but that's just a semantics argument.

Quote:

RogerRabbit said:
I would consider a 'generation' to begin with a successful spore mating. In my opinion, you could expand it until the cows come home, and it's still first generation, until you fruit it to obtain spores, which could begin the second generation.

Senescence is what you're thinking of when you expand the mycelium too far.
RR




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Re: G2G2G2G question [Re: wygram]
    #11265022 - 10/17/09 01:14 PM (8 years, 5 days ago)

Yeah, I don't like the term myself. But it's what we seem to be stuck with.
As long as we're clear about the topic- how far can you expand mycelium- then it doesn't really matter. The real answer is "as long as it keeps working".


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Re: G2G2G2G question [Re: Doc_T]
    #11265197 - 10/17/09 01:46 PM (8 years, 5 days ago)

yea i wonder about senseance all the time.  It is not a defenitive thing.  After so many cell divisions is when you reach it.  RR has said that he has done 4-5 "generations" without reaching it.  But it seems the only way that you can tell is by sampling the fruits


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Re: G2G2G2G question [Re: truskool]
    #11265220 - 10/17/09 01:51 PM (8 years, 5 days ago)

Right. And just as different people will live to different ages, so will different cultures. Senescence is just one thing to be aware of, that's all.


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