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Imorph
journeyman
Registered: 11/10/01
Posts: 146
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Storing extra MEA media?
#11260657 - 10/16/09 03:26 PM (14 years, 5 months ago) |
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hello everyone, i have a extra half liter of MEA liquid unsterilized. i would like to keep it until i need it. Can i store it in the freezer and unthaw it when i want to pc it without problems? anyone tips welcomed, thx
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PitcherCrab
Crescent Fresh
Registered: 12/02/06
Posts: 2,446
Loc: The bottom of the sea.
Last seen: 1 year, 3 months
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Re: Storing extra MEA media? [Re: Imorph]
#11261599 - 10/16/09 05:53 PM (14 years, 5 months ago) |
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I'd say PC it for 15 @ 45psi, then just store in a closet or something not in the fridge though, they are super dirty. You can PC with the lid nice and tight, just make sure that you let it cool ALL THE WAY before you open, or else it will pop. I've kept unused slants for 6 months plus without any problems.
-------------------- PC's LAGM 2021 (TOC & TWC)P. natalensis Growlog 2021Pans for PC Fall 2021 Growlog “I must not fear. Fear is the mind-killer. Fear is the little death that brings total obliteration. I will face my fear. I will permit it to pass over me and through me. And when it has gone past I will turn the inner eye to see its path. Where the fear has gone there will be nothing. Only I will remain.” - Bene Gesserit Litany Against Fear
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Imorph
journeyman
Registered: 11/10/01
Posts: 146
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Re: Storing extra MEA media? [Re: PitcherCrab]
#11262808 - 10/16/09 09:45 PM (14 years, 5 months ago) |
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thank you for responding Pitcher, i will use your logical suggestion if cant find another one.
i made a small boo boo. i mixed up a half liter of MEA when i really planned to use a half liter of Karo liquid....so now i have a half liter of unsterilized MEA.
Maybe i should have asked. ...is it possible to "freeze" Malt,Potatoes or Oatmeal agar's and pc them after unthawing ? all replys welcomed
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ratdog
wild capture trader
Registered: 08/16/09
Posts: 959
Loc: Colorado mount. top
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Re: Storing extra MEA media? [Re: Imorph]
#11262899 - 10/16/09 10:04 PM (14 years, 5 months ago) |
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i would cap it good and keep in the fridge
i mean your gunna have to warm it and pc it again right?
-------------------- some people just don't get it http://www.shroomery.org/forums/showflat.php/Number/11241796 so here is a video or two or three for you guys
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Imorph
journeyman
Registered: 11/10/01
Posts: 146
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Re: Storing extra MEA media? [Re: ratdog]
#11264079 - 10/17/09 05:22 AM (14 years, 5 months ago) |
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yeah,uh huh...but you're saying Fridge and i am saying Freezer Freezer....as in frozen stiff. i wonder if the nutriment agars can even survive a FREEZING then reconstitute back to proper semi solid gel form ?
maybe i ll commit this half liter to the freezer compartment then report the results of thawing to the forum ! take it ezy everybody and out all contams
Edited by Imorph (10/17/09 05:31 AM)
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Mycelio
Stranger
Registered: 06/24/08
Posts: 1,636
Loc: Berlin
Last seen: 5 months, 19 days
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Re: Storing extra MEA media? [Re: Imorph]
#11264259 - 10/17/09 06:58 AM (14 years, 5 months ago) |
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I once read a thread about creating ice cubes from the agar mixture and later throwing single cubes into agar dishes for PCing. Might be an idea for you too.
Carsten
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Imorph
journeyman
Registered: 11/10/01
Posts: 146
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Re: Storing extra MEA media? [Re: Mycelio]
#11264392 - 10/17/09 08:20 AM (14 years, 5 months ago) |
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Dunkah for the reply Mycelio... ok, apparently from your post then agar media can survive below 32 degrees F and still be useful .... i have 1 quest ...why did they make the agar into ice cubes first before PCing ??? out all contams! Imorph
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Mycelio
Stranger
Registered: 06/24/08
Posts: 1,636
Loc: Berlin
Last seen: 5 months, 19 days
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Re: Storing extra MEA media? [Re: Imorph]
#11264467 - 10/17/09 08:50 AM (14 years, 5 months ago) |
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Unfortunately I can't find this old thread. Perhaps somebody else remembers and can help?
Anyway, the cool thing was that one ice cube just had the proper volume for one petri dish, so a large amount of agar cubes could be prepared once and later taken out one by one, while the rest would stay frozen.
Carsten
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Imorph
journeyman
Registered: 11/10/01
Posts: 146
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Re: Storing extra MEA media? [Re: Mycelio]
#11264699 - 10/17/09 09:59 AM (14 years, 5 months ago) |
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oh ok!...thx again i just happen to have a cube tray in the frig...that's a good idea!...so when you decide to PC plates you already have the measured amount for each plate ...i have over poured plates on
a couple of occasions...this makes agar work more convenience... ah sweet serendipity!
i ll maybe be posting my small salvaged elm oyster grow in days or a week. thx and aveedazane, out all contams! Imorph
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