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Amazon Shop: Microscope

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Offlineasanaman
MYXOMOP

Registered: 08/11/05
Posts: 48
Loc: Europe
Last seen: 6 years, 11 months
Spores under a microscope
    #11260819 - 10/16/09 05:49 PM (8 years, 8 days ago)

Got a microscope today. And my spore prints are ready too. So, logically, did the thing they were bought for (the place I got them sells them explicitly as spores for microscopy :wink:)

P. cubensis "Cambodian" at x1000 magnification. Enjoy. And post yours! Is there a thread or database of spore images?


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InvisibleinskiM
Cortinariologist


Registered: 03/01/06
Posts: 4,760
Re: Spores under a microscope [Re: asanaman]
    #11261408 - 10/16/09 07:26 PM (8 years, 8 days ago)

Nice, you can see that the spores are collapsed, to view them better you should let them hydrate in a drop of water on the slide before placing the cover slip over them, I find leaving them for half an hour or so in water sufficiently hydrates them!
inski..


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OfflineRogerRabbitM
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Registered: 03/26/03
Posts: 42,018
Loc: USA Mountain Northwest
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Re: Spores under a microscope [Re: inski]
    #11261526 - 10/16/09 07:41 PM (8 years, 8 days ago)

You can germinate them on the slide too.  This is 400X.
RR


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InvisibleinskiM
Cortinariologist


Registered: 03/01/06
Posts: 4,760
Re: Spores under a microscope [Re: RogerRabbit]
    #11261703 - 10/16/09 08:05 PM (8 years, 8 days ago)

Nice shot Roger, do you add some form of sugar to the water for germination, I have seen it happen with plain water but maybe honey water would work better!
inski.


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Offlineasanaman
MYXOMOP

Registered: 08/11/05
Posts: 48
Loc: Europe
Last seen: 6 years, 11 months
Re: Spores under a microscope [Re: inski]
    #11263594 - 10/17/09 03:23 AM (8 years, 8 days ago)

Thanks for the hydration tip, I assumed them being collapsed was a feature of the spores.

I will definitely try to soak them in honeywater to see if that promotes germination.

Do you use a thin top cover glass above the specimen, or can that hinder possible mycelium growth?


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OfflineRogerRabbitM
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Re: Spores under a microscope [Re: asanaman]
    #11267170 - 10/17/09 08:13 PM (8 years, 7 days ago)

Karo is better because it's clear.  It doesn't cloud the slide the way honey does.  With very fresh spores, you'll start to see germination within twenty minutes to an hour.

You have to use a cover slip.  Otherwise, your spores would not all be in the same plane of focus.
RR


--------------------
Download Let's Grow Mushrooms



semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
Thomas Edison


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InvisibleMr. Mushrooms
Spore Print Collector
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Registered: 05/25/08
Posts: 13,018
Loc: Registered: 6/04/02
Re: Spores under a microscope [Re: asanaman]
    #11298834 - 10/22/09 01:03 PM (8 years, 2 days ago)

Quote:

asanaman said:
Is there a thread or database of spore images?





There is this: Spore microscopy photos

Enjoy.


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Amazon Shop: Microscope

Mushrooms, Mycology and Psychedelics >> Advanced Mycology

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