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OfflineKamek
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Grey Oyster Spores
    #11259845 - 10/16/09 02:57 PM (8 years, 1 month ago)

Is it true that Grey Oysters don't sporulate? Or just some strains?
I got this noobie grow-set and on the leaflet that came with it it says they don't drop spores.

Can someone tell me why this is? I was hoping I could make some spore prints so I can do my own grow in the future but it looks like that won't be happening.

I also got some Shiitake so I guess I'll just have to settle for growing Shiitake.


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InvisiblePitcherCrab
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Re: Grey Oyster Spores [Re: Kamek]
    #11261390 - 10/16/09 07:23 PM (8 years, 1 month ago)

Its probably just the strain... but that doesn't mean you can't clone the fruit you produce.


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Offlineflugelizor
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Re: Grey Oyster Spores [Re: PitcherCrab]
    #11261569 - 10/16/09 07:47 PM (8 years, 1 month ago)

Yeah, clone it!
Sporeless strains are good!
One of the negative sides of oysters is the heavy spore load.
If you grow them indoors, I have read that you can have bad reactions from inhaling the spores.


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OfflinebioBot

Registered: 05/02/09
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Re: Grey Oyster Spores [Re: Kamek]
    #11262134 - 10/16/09 09:31 PM (8 years, 1 month ago)

According to reports, Shiitake - although they do
drop sporeprints - are extremely difficult to cultivate
in such a way due to their very poor ability to form
fruiting strains from multispore.

It takes ages of isolations and is quite an undertaking.

About the grey oyster, take some tissue samples as early
as you can get them, and use those as your 'mother'
cultures.  If you wait all the way until mushrooms form,
it will have scenesed(aged) much farther than need be,
and you will end up with a less vigorous master culture.

:1up:


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OfflineKamek
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Re: Grey Oyster Spores [Re: bioBot]
    #11263577 - 10/17/09 03:16 AM (8 years, 1 month ago)

So I should just pick a pin as soon as it's big enough for a tissue sample?


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OfflinebioBot

Registered: 05/02/09
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Re: Grey Oyster Spores [Re: Kamek]
    #11273609 - 10/18/09 10:40 PM (8 years, 1 month ago)

If you're going to clone, wait until you see which
ones grow the biggest, otherwise you won't know what
you're getting.

To obtain a tissue sample for backup, you won't need
a mushroom.  Get a chunk of colonized substrate* that
seems to be growing vigorously, and obtain a sample
from that.  You won't know for sure what its genetic
makeup will be, but vigorous-dense growth is usually
a good indication of a candidate.

Once a plate is colonized, tissue may be saved long-
term in distilled water.  A sterile glass dropper is
used to pipe in sterile distilled water(SDW) to a culture
plate, and then the smooth tip is gently rubbed around
the mycelial mat to dislodge cells into the water.

You want only the tissue, not the nutrient.

The mixture is then piped-dripped into test tubes,
vacutainers, etc already prepared - partially filled
with SDW. One drip of mycelial solution may contain
millions of viable cells, so its good practice to dilute
it.

The test tubes or what-have-you must also of course
be airtight to prevent eventual evaporation. When going
to use, dip an instrument into the solution - like an
inoculation loop - then streak across a plate.

Another way to go would be to use part of the chunk
of colonized substrate* or mushroom sample to grow a
standard liquid culture. Not a long-term solution, but
well worth it.

:mushroom2:


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OfflineKamek
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Re: Grey Oyster Spores [Re: bioBot]
    #11305030 - 10/23/09 10:59 AM (8 years, 28 days ago)

Quote:

bioBot said:
To obtain a tissue sample for backup, you won't need
a mushroom.  Get a chunk of colonized substrate* that
seems to be growing vigorously, and obtain a sample
from that.  You won't know for sure what its genetic
makeup will be, but vigorous-dense growth is usually
a good indication of a candidate.




What do you mean with "backup". Are you talking about transferring a piece of colonised substrate to agar?


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OfflinebioBot

Registered: 05/02/09
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Re: Grey Oyster Spores [Re: Kamek]
    #11307542 - 10/23/09 07:10 PM (8 years, 28 days ago)

Yes - that is what I meant. :chemistry:


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